FastQCFastQC Report
Fri 17 Jun 2016
SRR1527340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472557
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC76691.6228730079122728No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT46740.9890870307708912No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38340.8113306966143766No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC26980.5709364161360428No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA24260.5133772222186953No Hit
ATCAACGGGTACTTCATTATTCTCATCTAGCAAACTGGATCCTTCAAATGTA19480.4122254034962978No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT14330.30324384148367284No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC9830.20801723389982582No Hit
ATCAACGGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATAAT9690.2050546283305506No Hit
ATCAACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG9680.20484301364703095No Hit
ATCAACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG9430.19955264655903945No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC9020.19087644453473337No Hit
ATCAACGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCT8470.17923763694115208No Hit
ATCAACGGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGC8000.16929174681572803No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC7720.16336653567717757No Hit
ATCAACGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT7660.1620968475760596No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA7520.15913424200678436No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG6780.14347475542632954No Hit
ATCAACGGGCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGC6670.14114699390761326No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT6580.13924246175593633No Hit
ATCAACAGGCAGAACACTGTTGTGGGACTATCCTTGTTTGGTCTTTGATTGT6410.1356450121361021No Hit
ATCAACGGGACTGTCCCTCAGCACTGCTCCTCTCTTGCTGATCTTTTAGCTC6230.13183594783274824No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT6180.13077787441514993No Hit
ATCAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT5950.12591073669419775No Hit
ATCAACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC5910.1250642779601191No Hit
ATCAACGGGACGGTAGAACACTGCAGCTGTCGCCAGGGTTACAGTGCGCATA5870.12421781922604044No Hit
ATCAACGGGACTTGACTGCAGAGGGAGCGCTGCTTGCTGAGTCAGAGGATGA5800.12273651644140283No Hit
ATCAACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA5620.11892745213804895No Hit
ATCAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT5500.11638807593581303No Hit
ATCAACGGGGCACCTGGTCCTCCGTCTGAGGGGTGGCTATTAATTATTCGGT5470.11575323188525405No Hit
ATCAACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC5430.11490677315117541No Hit
ATCAACGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGAT5380.11384869973357713No Hit
ATCAACGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC5240.11088609416430187No Hit
ATCAACGGGCAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGGAC5160.10919317669614459No Hit
ATCAACGGGACAGGTTTCAGGCCAGCTTTGATATCAAGGTCTAAAAAATAAA5040.10665380049390868No Hit
ATCAACGGGGGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAGCCTG5040.10665380049390868No Hit
ATCAACGGGGCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACG5020.10623057112686936No Hit
ATCAACGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC5010.10601895644334969No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTC4990.10559572707631038No Hit
ATCAACGGGGGCTGCAGCCAATCAGGGAGTGACACGTCCTAGGCGAAGGAGA4940.10453765365871207No Hit
ATCAACGGGGGGGCTGAAGAGATGGCTCAGAGGTTAAGAGCAGTGGCTACTC4900.10369119492463343No Hit
ATCAACGGGGTCCTACCCTCCAGCCAAGGTAGAATCTTGCCAAGCCTAGCCT4890.10347958024111378No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC4880.10326796555759411No Hit
ATCAACGGGATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCC4880.10326796555759411No Hit
ATCAACGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA4830.1022098921399958No Hit
ATCAACGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4780.10115181872239751No Hit
ATCAACGGGAAGCTCAGATATTCACAAGAAATGAGTTAAATCAGTTGTTGGC4750.10051697467183852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT750.046.00000429
CCCTATA551.8189894E-1246.00000436
CACGACG750.046.00000427
GTTACGA551.8189894E-1246.00000417
TACGAAT551.8189894E-1246.00000419
AGTTACG551.8189894E-1246.00000416
TTACGAA551.8189894E-1246.00000418
TCGGTAA551.8189894E-1246.00000411
ACGACGC750.046.00000428
TATCATT253.41408E-546.044
ATTTACG301.8590599E-646.021
CTTAACG301.8590599E-646.040
CTGCTAT253.41408E-546.040
CGGAATC253.41408E-546.019
AGGTACT253.41408E-546.010
ACTATAA206.307664E-446.010
GATACCG501.6370905E-1146.042
TCGTTAG405.5988494E-946.014
AAATCGA206.307664E-446.033
CGAACCT206.307664E-446.031
Produced by FastQC (version 0.11.5)