##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	472557
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.25460843877035	31.0	30.0	33.0	27.0	34.0
2	31.007063698135887	31.0	31.0	33.0	28.0	34.0
3	31.714097981830765	31.0	31.0	34.0	30.0	34.0
4	35.58768148604295	37.0	35.0	37.0	33.0	37.0
5	35.98949967940376	37.0	35.0	37.0	35.0	37.0
6	36.1580169164778	37.0	35.0	37.0	35.0	37.0
7	36.32110623691957	37.0	35.0	37.0	35.0	37.0
8	36.49979790797724	37.0	37.0	37.0	35.0	37.0
9	38.525697429093206	39.0	39.0	39.0	37.0	39.0
10	36.796631940697104	39.0	37.0	39.0	32.0	39.0
11	37.00845400660661	39.0	37.0	39.0	34.0	39.0
12	36.639709072133094	38.0	35.0	39.0	33.0	39.0
13	36.540108389040896	39.0	35.0	39.0	33.0	39.0
14	37.198503037728784	39.0	36.0	40.0	33.0	40.0
15	37.295052237084626	39.0	36.0	40.0	33.0	40.0
16	37.31689933701119	39.0	36.0	40.0	33.0	40.0
17	37.2342955452993	39.0	36.0	40.0	32.0	40.0
18	37.15689959094882	39.0	35.0	40.0	32.0	40.0
19	36.92900750597283	39.0	35.0	40.0	31.0	40.0
20	36.817014667013716	39.0	35.0	40.0	31.0	40.0
21	36.841566202595665	39.0	35.0	40.0	31.0	40.0
22	36.93415609122286	39.0	35.0	40.0	32.0	40.0
23	36.891031134868385	39.0	35.0	40.0	32.0	40.0
24	36.980745179946545	39.0	35.0	40.0	32.0	40.0
25	37.00088878167078	39.0	35.0	40.0	33.0	40.0
26	36.92165389572052	38.0	35.0	40.0	32.0	40.0
27	36.861991251848984	38.0	35.0	40.0	32.0	40.0
28	36.63330137951612	38.0	35.0	40.0	31.0	40.0
29	36.60530898918014	38.0	35.0	40.0	31.0	40.0
30	36.47657531260779	38.0	35.0	40.0	31.0	40.0
31	36.46338113709034	38.0	35.0	40.0	31.0	40.0
32	36.33498392786478	38.0	35.0	40.0	31.0	40.0
33	36.11501469663977	38.0	35.0	40.0	31.0	40.0
34	35.603385411706945	38.0	34.0	40.0	29.0	40.0
35	35.556330770679516	38.0	34.0	40.0	29.0	40.0
36	35.56349181156982	38.0	34.0	40.0	29.0	40.0
37	35.68187118167756	38.0	34.0	40.0	30.0	40.0
38	35.71430959651428	38.0	34.0	40.0	30.0	40.0
39	35.607399742253314	37.0	34.0	40.0	30.0	40.0
40	35.949587033945114	38.0	35.0	40.0	31.0	40.0
41	36.11466341626513	38.0	35.0	40.0	31.0	40.0
42	36.150229496124275	38.0	35.0	40.0	31.0	40.0
43	36.099717071168136	38.0	35.0	40.0	31.0	40.0
44	35.993221981686865	38.0	35.0	40.0	31.0	40.0
45	35.86895125878994	37.0	35.0	40.0	31.0	40.0
46	35.55776975052745	37.0	34.0	40.0	30.0	40.0
47	35.464650825191455	37.0	34.0	40.0	30.0	40.0
48	35.34902879440999	37.0	34.0	40.0	30.0	40.0
49	35.26475747899195	36.0	34.0	40.0	30.0	40.0
50	35.12499232896772	36.0	34.0	40.0	29.0	40.0
51	35.0234363261998	36.0	34.0	39.0	29.0	40.0
52	34.63198725233146	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	8.0
15	7.0
16	50.0
17	61.0
18	103.0
19	169.0
20	272.0
21	471.0
22	718.0
23	982.0
24	1495.0
25	2070.0
26	2855.0
27	3795.0
28	5204.0
29	6870.0
30	8961.0
31	12445.0
32	17662.0
33	26868.0
34	48254.0
35	36729.0
36	56290.0
37	91818.0
38	136892.0
39	11505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.82913595608572	1.0138459487426914	0.08316457062322641	0.07385352454836136
8	98.65730483306776	0.2837752905998641	0.10009374530479921	0.9588261310275797
9	96.74346163531595	2.044409457483436	0.3341395852775432	0.8779893219230697
10	50.84317870648408	35.4092733786612	4.171348641539539	9.576199273315176
11	37.35549362299152	20.6059798077269	23.850456135450326	18.188070433831264
12	34.1004365610921	15.342699399225914	25.477561437032993	25.079302602648994
13	26.319787877441243	15.283658902523928	30.647942999468846	27.748610220565983
14	25.066182492270773	17.472389574167774	33.406128784464094	24.05529914909736
15	27.68999295323104	18.47628963278504	29.471788588466573	24.361928825517346
16	32.57596438101647	21.230877968160456	24.848007753562005	21.34514989726107
17	32.16056475726738	20.49826793381539	25.712242121056295	21.628925187860933
18	30.66635347693506	20.637933624938366	25.69108065270433	23.004632245422247
19	29.601508389464126	24.19221384933458	23.86802015418246	22.338257607018836
20	29.830263862348882	24.211894014901905	24.33357245792571	21.624269664823505
21	28.04973791521446	24.943022746462333	25.787365333705775	21.21987400461743
22	27.466739462117793	21.977242956934294	27.905839930421095	22.650177650526814
23	28.82043859259306	23.06282628339015	25.535332245633864	22.581402878382924
24	29.99236070992494	21.186650499304847	24.348173871088566	24.472814919681646
25	28.44122507972583	22.34100859790459	22.314556762464637	26.903209559904944
26	23.79945699672209	22.971620354793178	25.47459883146372	27.754323817021014
27	22.84443992999786	20.968264145912556	24.78685111002482	31.40044481406476
28	25.699122010678078	24.6025347206792	22.736304826719316	26.962038441923408
29	29.094479607751023	23.793743400267058	22.14209079539611	24.96968619658581
30	28.2492905617735	21.080208313494456	25.394608481093282	25.27589264363876
31	32.14173105043413	20.56048265077017	24.083020672638433	23.21476562615727
32	32.72494111821431	20.36897136218488	24.679774080163874	22.226313439436936
33	33.317673846752875	20.446633951036596	24.29336566805698	21.94232653415355
34	26.503257808052783	23.583398404848516	28.887520447268795	21.025823339829905
35	25.554800796517668	27.852724644857656	26.095264698226885	20.497209860397795
36	26.080663285064027	28.632313138944088	25.411326041091336	19.875697534900553
37	29.555376388456843	27.324534394792586	21.608610178243048	21.511479038507524
38	28.063704484326756	29.032053276112723	22.488504032317795	20.415738207242722
39	29.78603639349327	25.473329143362598	22.28704685360708	22.453587609537053
40	25.30573031401503	24.222686363761408	23.63630207572843	26.83528124649513
41	24.612057381437584	27.055783746722618	21.73198153873501	26.600177333104792
42	23.003150942637607	23.01225037402895	25.072107703409323	28.912490979924115
43	24.050432011376405	23.80474736381008	22.698214183685778	29.446606441127738
44	24.541378077142017	21.8352495042926	24.99931225227856	28.624060166286817
45	26.38200259439602	21.572635682044705	24.305427705017596	27.73993401854168
46	26.566318983741645	25.264465450728697	23.52287660536189	24.646338960167768
47	24.50815457182943	24.56402084827862	24.68210184168259	26.24572273820936
48	26.39406463135664	24.077518690866924	25.5723648152498	23.956051862526635
49	26.728204216634182	22.788150424181634	25.5486639706956	24.934981388488584
50	24.504133892842557	21.39403288915411	26.79380476852528	27.30802844947805
51	24.08513681947363	22.744769414060105	27.50271395831614	25.667379808150127
52	22.31011285411072	23.26068601248103	27.827965726885857	26.601235406522388
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	3.0
4	6.0
5	5.0
6	4.0
7	5.0
8	6.0
9	55.5
10	105.0
11	217.5
12	330.0
13	424.5
14	518.0
15	517.0
16	690.5
17	864.0
18	821.5
19	779.0
20	1028.5
21	1278.0
22	1119.5
23	961.0
24	1230.0
25	1499.0
26	1562.0
27	1625.0
28	2443.0
29	3261.0
30	4361.5
31	5462.0
32	6844.0
33	8226.0
34	8034.5
35	7843.0
36	10267.0
37	12691.0
38	12792.0
39	12683.5
40	12474.0
41	14106.5
42	15739.0
43	16696.5
44	17654.0
45	18977.5
46	20301.0
47	23317.5
48	26334.0
49	26370.0
50	26406.0
51	27163.5
52	27921.0
53	28384.5
54	28848.0
55	36439.5
56	44031.0
57	41986.5
58	39942.0
59	40337.5
60	40733.0
61	42434.0
62	44135.0
63	34045.0
64	20201.5
65	16448.0
66	12395.5
67	8343.0
68	7717.0
69	7091.0
70	6367.0
71	5643.0
72	4550.5
73	3458.0
74	2863.0
75	2268.0
76	1810.5
77	1353.0
78	894.5
79	436.0
80	280.5
81	125.0
82	75.5
83	26.0
84	19.5
85	13.0
86	9.5
87	6.0
88	5.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	472557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.197923213495939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.75972691996431	7.012064153107456
2	9.788134713950738	1.996372924324473
3	3.7289120375173788	1.1408147588544875
4	1.7409889813450645	0.7101788778919792
5	0.8445560374343757	0.4306358809625082
6	0.5561204374260754	0.3402764110996134
7	0.45651677699155446	0.32588661262027646
8	0.37558880288850616	0.3064180617364678
9	0.3278620489302982	0.3009160799649566
>10	8.723620593056795	24.13528949946779
>50	2.936232906559316	20.483031676601975
>100	1.6828868460915938	33.20805744068969
>500	0.06432736403062812	4.386984004046073
>1k	0.012450457554315123	3.6002006107199764
>5k	0.0020750762590525202	1.6228730079122728
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7669	1.6228730079122728	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4674	0.9890870307708912	No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3834	0.8113306966143766	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	2698	0.5709364161360428	No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2426	0.5133772222186953	No Hit
ATCAACGGGTACTTCATTATTCTCATCTAGCAAACTGGATCCTTCAAATGTA	1948	0.4122254034962978	No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1433	0.30324384148367284	No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	983	0.20801723389982582	No Hit
ATCAACGGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATAAT	969	0.2050546283305506	No Hit
ATCAACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	968	0.20484301364703095	No Hit
ATCAACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	943	0.19955264655903945	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	902	0.19087644453473337	No Hit
ATCAACGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCT	847	0.17923763694115208	No Hit
ATCAACGGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGC	800	0.16929174681572803	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	772	0.16336653567717757	No Hit
ATCAACGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	766	0.1620968475760596	No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	752	0.15913424200678436	No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	678	0.14347475542632954	No Hit
ATCAACGGGCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGC	667	0.14114699390761326	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	658	0.13924246175593633	No Hit
ATCAACAGGCAGAACACTGTTGTGGGACTATCCTTGTTTGGTCTTTGATTGT	641	0.1356450121361021	No Hit
ATCAACGGGACTGTCCCTCAGCACTGCTCCTCTCTTGCTGATCTTTTAGCTC	623	0.13183594783274824	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	618	0.13077787441514993	No Hit
ATCAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	595	0.12591073669419775	No Hit
ATCAACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	591	0.1250642779601191	No Hit
ATCAACGGGACGGTAGAACACTGCAGCTGTCGCCAGGGTTACAGTGCGCATA	587	0.12421781922604044	No Hit
ATCAACGGGACTTGACTGCAGAGGGAGCGCTGCTTGCTGAGTCAGAGGATGA	580	0.12273651644140283	No Hit
ATCAACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	562	0.11892745213804895	No Hit
ATCAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	550	0.11638807593581303	No Hit
ATCAACGGGGCACCTGGTCCTCCGTCTGAGGGGTGGCTATTAATTATTCGGT	547	0.11575323188525405	No Hit
ATCAACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	543	0.11490677315117541	No Hit
ATCAACGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGAT	538	0.11384869973357713	No Hit
ATCAACGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC	524	0.11088609416430187	No Hit
ATCAACGGGCAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGGAC	516	0.10919317669614459	No Hit
ATCAACGGGACAGGTTTCAGGCCAGCTTTGATATCAAGGTCTAAAAAATAAA	504	0.10665380049390868	No Hit
ATCAACGGGGGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAGCCTG	504	0.10665380049390868	No Hit
ATCAACGGGGCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACG	502	0.10623057112686936	No Hit
ATCAACGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC	501	0.10601895644334969	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTC	499	0.10559572707631038	No Hit
ATCAACGGGGGCTGCAGCCAATCAGGGAGTGACACGTCCTAGGCGAAGGAGA	494	0.10453765365871207	No Hit
ATCAACGGGGGGGCTGAAGAGATGGCTCAGAGGTTAAGAGCAGTGGCTACTC	490	0.10369119492463343	No Hit
ATCAACGGGGTCCTACCCTCCAGCCAAGGTAGAATCTTGCCAAGCCTAGCCT	489	0.10347958024111378	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	488	0.10326796555759411	No Hit
ATCAACGGGATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCC	488	0.10326796555759411	No Hit
ATCAACGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	483	0.1022098921399958	No Hit
ATCAACGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	478	0.10115181872239751	No Hit
ATCAACGGGAAGCTCAGATATTCACAAGAAATGAGTTAAATCAGTTGTTGGC	475	0.10051697467183852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0025393762022359206	0.0	0.0	0.0	0.0
8	0.003385834936314561	0.0	0.0	0.0	0.0
9	0.013966569112297563	0.0	0.0	0.0	0.0
10	0.019891780250848045	0.0	0.0	0.0	0.0
11	0.02856798227515411	0.0	0.0	0.0	0.0
12	0.03618611088186187	0.0	0.0	0.0	0.0
13	0.041899707336892696	0.0	0.0	0.0	0.0
14	0.04740168910840385	0.0	0.0	0.0	0.0
15	0.05650112049974924	0.0	0.0	0.0	0.0
16	0.13437532403498414	0.0	0.0	0.0	0.0
17	0.14157022327465257	0.0	0.0	0.0	0.0
18	0.1468605903626441	0.0	0.0	0.0	0.0
19	0.1563832511210288	0.0	0.0	0.0	0.0
20	0.1642129944112562	0.0	0.0	0.0	0.0
21	0.1716195083344443	0.0	0.0	0.0	0.0
22	0.17839117820707343	0.0	0.0	0.0	0.0
23	0.18368154529506495	0.0	0.0	0.0	0.0
24	0.19045321516769406	0.0	0.0	0.0	0.0
25	0.1965900409897642	0.0	0.0	0.0	0.0
26	0.20547785769758992	0.0	0.0	0.0	0.0
27	0.22283026174620205	0.0	0.0	0.0	0.0
28	0.23362261060570472	0.0	0.0	0.0	0.0
29	0.2410291245288928	0.0	0.0	0.0	0.0
30	0.24864725313560057	0.0	0.0	0.0	0.0
31	0.2592279873115836	0.0	0.0	0.0	0.0
32	0.2695971068040469	0.0	0.0	0.0	0.0
33	0.278273308828353	0.0	0.0	0.0	0.0
34	0.288854043004336	0.0	0.0	0.0	0.0
35	0.3004928505979173	0.0	0.0	0.0	0.0
36	0.31657556654541147	0.0	0.0	0.0	0.0
37	0.3269446860378748	0.0	0.0	0.0	0.0
38	0.33816026426441675	0.0	0.0	0.0	0.0
39	0.34895261312391945	0.0	0.0	0.0	0.0
40	0.3578404298317452	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGCT	75	0.0	46.000004	29
CCCTATA	55	1.8189894E-12	46.000004	36
CACGACG	75	0.0	46.000004	27
GTTACGA	55	1.8189894E-12	46.000004	17
TACGAAT	55	1.8189894E-12	46.000004	19
AGTTACG	55	1.8189894E-12	46.000004	16
TTACGAA	55	1.8189894E-12	46.000004	18
TCGGTAA	55	1.8189894E-12	46.000004	11
ACGACGC	75	0.0	46.000004	28
TATCATT	25	3.41408E-5	46.0	44
ATTTACG	30	1.8590599E-6	46.0	21
CTTAACG	30	1.8590599E-6	46.0	40
CTGCTAT	25	3.41408E-5	46.0	40
CGGAATC	25	3.41408E-5	46.0	19
AGGTACT	25	3.41408E-5	46.0	10
ACTATAA	20	6.307664E-4	46.0	10
GATACCG	50	1.6370905E-11	46.0	42
TCGTTAG	40	5.5988494E-9	46.0	14
AAATCGA	20	6.307664E-4	46.0	33
CGAACCT	20	6.307664E-4	46.0	31
>>END_MODULE