FastQCFastQC Report
Fri 17 Jun 2016
SRR1527339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506201
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC69701.3769233960422835No Hit
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT29770.5881063055979739No Hit
GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC28940.5717096568359209No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG27020.5337800596996055No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAA22550.4454752163666212No Hit
GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC19610.3873955207516382No Hit
GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC19530.3858151208709584No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG18730.3700111220641603No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG18370.36289932260110114No Hit
GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG17480.3453173739285383No Hit
GCCCTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA14280.2821013787013459No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA13420.26511207998403796No Hit
GCCCTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA13070.2581978305060638No Hit
GCCCTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG9910.19577203521921135No Hit
GCCCTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT9840.1943891853236165No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT9600.1896479856815771No Hit
GCCCTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG8820.17423908684494896No Hit
GCCCTCGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT8260.16317628768019027No Hit
GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC8240.16278118771002034No Hit
GCCCTCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA7990.15784243808289591No Hit
GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT7690.15191593853034663No Hit
GCCCTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA7360.1453967890225424No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC7030.13887763951473822No Hit
GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG6580.12998789018591428No Hit
GCCCTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC6490.1282099403201495No Hit
GCCCTCGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGG6410.1266295404394697No Hit
GCCCTCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG6330.12504914055878988No Hit
GCCCTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC6310.12465404058861994No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT6280.124061390633365No Hit
GCCCTCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT6260.12366629066319507No Hit
GCCCTCGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6250.12346874067811008No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT6230.12307364070794014No Hit
GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT6170.12188834079743026No Hit
GCCCTCGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC5980.11813489108081572No Hit
GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA5810.11477654133437112No Hit
GCCCTCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA5780.11418389137911619No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC5770.11398634139403122No Hit
GCCCTCGGGGGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTTG5660.1118132915580965No Hit
GCCCTCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG5580.11023289167741669No Hit
GCCCTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA5580.11023289167741669No Hit
GCCCTCGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA5480.1082573918265669No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC5450.107664741871312No Hit
GCCCTCGGGGGGGTTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG5400.1066769919458871No Hit
GCCCTCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAA5380.10628189197571716No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACT5370.10608434199063219No Hit
GCCCTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC5300.10470149209503735No Hit
GCCCTCGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA5240.1035161921845275No Hit
GCCCTCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC5140.10154069233367773No Hit
GCCCTCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG5120.10114559236350779No Hit
GCCCTCATTGCTCTTTGCCTGTCTCTGCTTTATGCCTGGAAAGCTTTCCA5080.10035539242316788No Hit
GCCCTCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC5080.10035539242316788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACA207.852301E-444.00000424
TAATACG207.852301E-444.00000430
TGATAAT408.296411E-944.00000428
ACCGGAC207.852301E-444.00000441
CTCGTTT207.852301E-444.00000439
AACCCTC207.852301E-444.00000443
CGTTGTA207.852301E-444.00000427
TCTTAAC207.852301E-444.00000423
ACCGAAC408.296411E-944.00000414
GCACGAC408.296411E-944.00000412
TACATTA207.852301E-444.00000418
TATGAGG207.852301E-444.00000427
CTAACGT207.852301E-444.00000436
TCTACTT207.852301E-444.00000412
AATCTTG207.852301E-444.00000436
GATATAG207.852301E-444.00000430
ATAAGGA207.852301E-444.00000416
ACTAAAG207.852301E-444.00000420
CATGTTA207.852301E-444.00000429
TAGATAT207.852301E-444.00000420
Produced by FastQC (version 0.11.5)