##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527339_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	506201
Sequences flagged as poor quality	0
Sequence length	50
%GC	63
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.878516636671993	31.0	31.0	34.0	28.0	34.0
2	31.20053298985976	31.0	31.0	34.0	28.0	34.0
3	32.254416723791536	34.0	31.0	34.0	30.0	34.0
4	36.20856537225331	37.0	37.0	37.0	35.0	37.0
5	35.93197366263599	37.0	35.0	37.0	35.0	37.0
6	36.242757323671825	37.0	37.0	37.0	35.0	37.0
7	36.110926292125065	37.0	35.0	37.0	35.0	37.0
8	36.33532924668264	37.0	37.0	37.0	35.0	37.0
9	38.312691993891754	39.0	39.0	39.0	37.0	39.0
10	37.08343128520094	39.0	37.0	39.0	34.0	39.0
11	36.69132222180517	38.0	37.0	39.0	32.0	39.0
12	36.19346267589357	37.0	35.0	39.0	32.0	39.0
13	36.29615310914044	38.0	35.0	39.0	32.0	39.0
14	37.13226366601409	39.0	36.0	40.0	33.0	40.0
15	37.19985539341092	39.0	36.0	40.0	33.0	40.0
16	37.3322237609171	39.0	36.0	40.0	33.0	40.0
17	37.237378037577955	39.0	36.0	40.0	33.0	40.0
18	37.08831867183194	39.0	35.0	40.0	33.0	40.0
19	36.998470963115444	39.0	35.0	40.0	32.0	40.0
20	36.86090505550167	38.0	35.0	40.0	32.0	40.0
21	36.761217382028086	38.0	35.0	40.0	32.0	40.0
22	36.565858226277705	38.0	35.0	40.0	31.0	40.0
23	36.32498157846389	38.0	35.0	40.0	31.0	40.0
24	36.2211986937995	38.0	35.0	40.0	31.0	40.0
25	35.95818657015691	38.0	34.0	40.0	31.0	40.0
26	35.40223547563122	37.0	34.0	39.0	30.0	40.0
27	35.24528991448061	37.0	34.0	39.0	30.0	40.0
28	34.97546429185245	37.0	34.0	38.0	29.0	40.0
29	34.618234653823286	36.0	33.0	38.0	29.0	40.0
30	34.28680701934607	36.0	33.0	38.0	27.0	40.0
31	34.07429854939046	36.0	33.0	38.0	27.0	39.0
32	33.87978885857594	35.0	33.0	38.0	27.0	39.0
33	33.238168237518295	35.0	32.0	38.0	25.0	39.0
34	32.676292618939904	34.0	31.0	38.0	23.0	39.0
35	32.52701397271044	34.0	31.0	37.0	23.0	38.0
36	32.303577037579934	34.0	31.0	37.0	24.0	38.0
37	31.966884696000207	34.0	30.0	36.0	23.0	38.0
38	31.592246953285354	33.0	30.0	36.0	23.0	38.0
39	30.83292210011438	33.0	29.0	35.0	21.0	38.0
40	30.501340376648802	33.0	29.0	35.0	21.0	38.0
41	30.231145335548526	32.0	28.0	35.0	21.0	37.0
42	29.878645044162297	32.0	27.0	34.0	20.0	37.0
43	29.310250276076104	31.0	27.0	34.0	19.0	36.0
44	28.729781252901514	31.0	26.0	34.0	18.0	36.0
45	28.372602977868475	30.0	25.0	33.0	18.0	36.0
46	28.087103739423668	30.0	24.0	33.0	18.0	35.0
47	27.753827037086058	30.0	24.0	33.0	18.0	35.0
48	27.095776183768898	29.0	23.0	32.0	16.0	34.0
49	26.45081894346317	29.0	23.0	32.0	14.0	34.0
50	29.83508329695121	32.0	27.0	34.0	20.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	26.0
12	40.0
13	76.0
14	113.0
15	166.0
16	228.0
17	389.0
18	506.0
19	740.0
20	1023.0
21	1442.0
22	2142.0
23	2951.0
24	4013.0
25	5318.0
26	6596.0
27	8753.0
28	11446.0
29	14341.0
30	18652.0
31	25813.0
32	39994.0
33	59902.0
34	73595.0
35	107486.0
36	101681.0
37	18361.0
38	402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.09146959409405	0.6258383527492043	0.41959616832048924	0.8630958848362607
8	98.16811898830701	0.8433408863277632	0.5916622053295035	0.39687792003571704
9	97.13473501632751	0.9855768755889459	0.5254829603260365	1.3542051477575114
10	67.95521936938094	21.133304754435493	3.887981256457415	7.023494619726156
11	40.61607938348601	26.689595634935532	14.527825903149145	18.166499078429318
12	35.40530342689959	13.456709884018403	26.345266010932416	24.79272067814959
13	25.20876094673855	16.20285222668466	27.998561836108582	30.589824990468212
14	26.19097947258105	17.216678750140755	29.415192779152942	27.17714899812525
15	27.4552993771249	16.51893220282062	28.301010863273678	27.724757556780805
16	32.103255426204214	17.404153685986394	24.904929069677856	25.587661818131537
17	33.41755547697456	18.15820197905575	22.673404438157966	25.750838105811724
18	31.856713044818164	19.236232247664464	21.5131933757539	27.393861331763468
19	31.660150809658617	19.59636587047438	21.37056228652255	27.37292103334446
20	32.08527837756148	20.951558768157312	20.322757165631835	26.64040568864937
21	33.421506476676264	20.188028075803878	22.656415139440657	23.7340503080792
22	32.609576037977014	17.040464163444955	24.314452164258864	26.03550763431917
23	31.428424677153938	19.43615283257046	21.21271984843965	27.92270264183595
24	33.40273922809319	18.998777165592323	21.596559469459763	26.001924136854726
25	29.21072854458999	18.274954020240973	21.333620439311655	31.180696995857378
26	29.226334993411708	18.53651020049348	23.540846422666096	28.696308383428715
27	30.11491482632393	16.226360674909767	21.732078759228056	31.926645739538245
28	30.58962744048313	22.351002862499282	18.988307016382823	28.071062680634768
29	32.61787313735058	19.889727598325564	19.99818254013722	27.494216724186636
30	29.972481287077663	19.653260266178847	21.611573268326218	28.762685178417268
31	35.86124879247571	18.180722677355437	19.529593975515656	26.42843455465319
32	36.99735875669941	18.60091149563118	19.15108820409284	25.250641543576563
33	35.84860559343028	18.51477970213413	21.205608048976593	24.431006655458997
34	31.62538201228366	20.890120722795885	24.220220821373328	23.26427644354713
35	31.30752408628193	24.47841865187939	22.009636488272445	22.20442077356623
36	31.62972811195553	25.248468493740628	20.007269839451126	23.114533554852716
37	32.63426978611263	25.130728702629984	19.458278430899977	22.776723080357407
38	34.40688580228012	23.032550311042453	20.103871782157682	22.456692104519746
39	34.88811756594713	21.32413804002758	18.088466834320755	25.69927755970454
40	30.967738111935773	20.87846527367587	20.81169337871715	27.342103235671207
41	29.493027473276424	21.3606847872683	21.207978648797614	27.938309090657665
42	26.983352462756887	19.588661421056063	22.370955410992867	31.057030705194183
43	28.27987301486959	18.08905948427601	24.348430761693475	29.282636739160928
44	27.94640864004615	16.65287109270823	23.66648821318014	31.73423205406548
45	29.46596312531978	18.35594951412581	24.01358353697444	28.164503823579963
46	28.944036064725275	20.575621146540605	22.9240953692308	27.556247419503322
47	28.63961153770933	19.40632278482263	23.539266022785416	28.414799654682625
48	31.21921924294895	18.710946837323515	23.66767351309065	26.402160406636888
49	29.350989034000328	19.04460876213204	24.104258980128446	27.500143223739187
50	28.442851752564692	20.028802787825388	22.923107619305373	28.605237840304543
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	16.0
10	32.0
11	18.5
12	5.0
13	5.5
14	6.0
15	13.0
16	20.0
17	48.0
18	76.0
19	115.0
20	154.0
21	164.0
22	174.0
23	258.5
24	343.0
25	449.0
26	555.0
27	582.0
28	609.0
29	745.0
30	881.0
31	1062.5
32	1244.0
33	1312.0
34	1380.0
35	1548.0
36	1716.0
37	1987.5
38	2259.0
39	2864.5
40	3470.0
41	4880.0
42	6290.0
43	6310.5
44	6331.0
45	6598.5
46	6866.0
47	6800.5
48	6735.0
49	7769.0
50	8803.0
51	10424.5
52	12046.0
53	13317.0
54	14588.0
55	16925.5
56	19263.0
57	20926.0
58	22589.0
59	27772.0
60	32955.0
61	40175.5
62	47396.0
63	48711.0
64	50026.0
65	55021.0
66	60016.0
67	57315.0
68	54614.0
69	49249.5
70	43885.0
71	38899.5
72	33914.0
73	27075.0
74	20236.0
75	18712.0
76	17188.0
77	13709.0
78	10230.0
79	11074.0
80	11918.0
81	8417.5
82	4917.0
83	3136.5
84	1356.0
85	1187.5
86	1019.0
87	546.0
88	73.0
89	45.0
90	17.0
91	10.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	506201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.199892927908083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.88286032276015	10.491287057907828
2	8.462715637173067	2.4033931185438195
3	2.949360044518642	1.256417905140448
4	1.3745130773511407	0.7807175410558256
5	0.7609905397885365	0.5402992092074097
6	0.5411797440178074	0.46108166518833427
7	0.422927100723428	0.42038636826082926
8	0.33110740122426263	0.37613517160179455
9	0.3283249860879243	0.41959616832048924
>10	8.146911519198664	30.714479031056836
>50	1.864218141346689	17.937341095730748
>100	0.862548692264886	23.061590158849942
>500	0.05425709515859766	4.96443112518545
>1k	0.01669449081803005	4.795920987907965
>5k	0.0013912075681691708	1.3769233960422835
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6970	1.3769233960422835	No Hit
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2977	0.5881063055979739	No Hit
GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2894	0.5717096568359209	No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2702	0.5337800596996055	No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAA	2255	0.4454752163666212	No Hit
GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1961	0.3873955207516382	No Hit
GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1953	0.3858151208709584	No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1873	0.3700111220641603	No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1837	0.36289932260110114	No Hit
GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1748	0.3453173739285383	No Hit
GCCCTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	1428	0.2821013787013459	No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1342	0.26511207998403796	No Hit
GCCCTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1307	0.2581978305060638	No Hit
GCCCTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	991	0.19577203521921135	No Hit
GCCCTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	984	0.1943891853236165	No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	960	0.1896479856815771	No Hit
GCCCTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	882	0.17423908684494896	No Hit
GCCCTCGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT	826	0.16317628768019027	No Hit
GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	824	0.16278118771002034	No Hit
GCCCTCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA	799	0.15784243808289591	No Hit
GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	769	0.15191593853034663	No Hit
GCCCTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	736	0.1453967890225424	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	703	0.13887763951473822	No Hit
GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	658	0.12998789018591428	No Hit
GCCCTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	649	0.1282099403201495	No Hit
GCCCTCGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGG	641	0.1266295404394697	No Hit
GCCCTCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG	633	0.12504914055878988	No Hit
GCCCTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	631	0.12465404058861994	No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT	628	0.124061390633365	No Hit
GCCCTCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	626	0.12366629066319507	No Hit
GCCCTCGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	625	0.12346874067811008	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	623	0.12307364070794014	No Hit
GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	617	0.12188834079743026	No Hit
GCCCTCGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC	598	0.11813489108081572	No Hit
GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	581	0.11477654133437112	No Hit
GCCCTCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA	578	0.11418389137911619	No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	577	0.11398634139403122	No Hit
GCCCTCGGGGGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTTG	566	0.1118132915580965	No Hit
GCCCTCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	558	0.11023289167741669	No Hit
GCCCTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	558	0.11023289167741669	No Hit
GCCCTCGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA	548	0.1082573918265669	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	545	0.107664741871312	No Hit
GCCCTCGGGGGGGTTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	540	0.1066769919458871	No Hit
GCCCTCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAA	538	0.10628189197571716	No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACT	537	0.10608434199063219	No Hit
GCCCTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	530	0.10470149209503735	No Hit
GCCCTCGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	524	0.1035161921845275	No Hit
GCCCTCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC	514	0.10154069233367773	No Hit
GCCCTCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	512	0.10114559236350779	No Hit
GCCCTCATTGCTCTTTGCCTGTCTCTGCTTTATGCCTGGAAAGCTTTCCA	508	0.10035539242316788	No Hit
GCCCTCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC	508	0.10035539242316788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.9754998508497612E-4	0.0	0.0	0.0	0.0
9	5.926499552549283E-4	0.0	0.0	0.0	0.0
10	0.009087299313908902	0.0	0.0	0.0	0.0
11	0.029830047747831396	0.0	0.0	0.0	0.0
12	0.041683046852929964	0.0	0.0	0.0	0.0
13	0.05610419576413322	0.0	0.0	0.0	0.0
14	0.07763714413839562	0.0	0.0	0.0	0.0
15	0.09067544315400404	0.0	0.0	0.0	0.0
16	0.10371374216961246	0.0	0.0	0.0	0.0
17	0.11556674127471103	0.0	0.0	0.0	0.0
18	0.12445649060353496	0.0	0.0	0.0	0.0
19	0.13670458967880347	0.0	0.0	0.0	0.0
20	0.14539678902254244	0.0	0.0	0.0	0.0
21	0.1546816383215363	0.0	0.0	0.0	0.0
22	0.16179343778459546	0.0	0.0	0.0	0.0
23	0.17186848702392923	0.0	0.0	0.0	0.0
24	0.18490678603953764	0.0	0.0	0.0	0.0
25	0.19320388541310665	0.0	0.0	0.0	0.0
26	0.20426668457786532	0.0	0.0	0.0	0.0
27	0.21631723366804886	0.0	0.0	0.0	0.0
28	0.3121289764342623	0.0	0.0	0.0	0.0
29	0.3257599254051256	0.0	0.0	0.0	0.0
30	0.35815812295906174	0.0	0.0	0.0	0.0
31	0.36902337213873543	0.0	0.0	0.0	0.0
32	0.38087637124383394	0.0	0.0	0.0	0.0
33	0.38956857058757294	0.0	0.0	0.0	0.0
34	0.4016191196777565	0.0	0.0	0.0	0.0
35	0.45298211579985026	0.0	0.0	0.0	0.0
36	0.4695763145469882	0.0	0.0	0.0	0.0
37	0.4816268636371718	0.0	0.0	0.0	0.0
38	0.48952886304057086	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACA	20	7.852301E-4	44.000004	24
TAATACG	20	7.852301E-4	44.000004	30
TGATAAT	40	8.296411E-9	44.000004	28
ACCGGAC	20	7.852301E-4	44.000004	41
CTCGTTT	20	7.852301E-4	44.000004	39
AACCCTC	20	7.852301E-4	44.000004	43
CGTTGTA	20	7.852301E-4	44.000004	27
TCTTAAC	20	7.852301E-4	44.000004	23
ACCGAAC	40	8.296411E-9	44.000004	14
GCACGAC	40	8.296411E-9	44.000004	12
TACATTA	20	7.852301E-4	44.000004	18
TATGAGG	20	7.852301E-4	44.000004	27
CTAACGT	20	7.852301E-4	44.000004	36
TCTACTT	20	7.852301E-4	44.000004	12
AATCTTG	20	7.852301E-4	44.000004	36
GATATAG	20	7.852301E-4	44.000004	30
ATAAGGA	20	7.852301E-4	44.000004	16
ACTAAAG	20	7.852301E-4	44.000004	20
CATGTTA	20	7.852301E-4	44.000004	29
TAGATAT	20	7.852301E-4	44.000004	20
>>END_MODULE