FastQCFastQC Report
Fri 17 Jun 2016
SRR1527338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458703
Sequences flagged as poor quality0
Sequence length52
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC62081.3533811638467592No Hit
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG28850.6289472708920587No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG26330.574009762308073No Hit
GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT25780.5620194330536317No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATA19640.4281637573767776No Hit
GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19460.42423964962077854No Hit
GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC18810.4100692605018934No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT17800.38805065587101023No Hit
GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA17340.3780223804945684No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT15300.3335491592599133No Hit
GCCCTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG13280.28951194999814694No Hit
GCCCTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG13050.284497812309926No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC12680.27643159081148366No Hit
GCCCTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG9360.20405360331194694No Hit
GCCCTCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT8740.1905372321523949No Hit
GCCCTCGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA8400.18312502861328572No Hit
GCCCTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8240.17963693283017554No Hit
GCCCTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT7550.16459451976551276No Hit
GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG7530.16415850779262398No Hit
GCCCTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG7150.15587428030773726No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC6960.1517321665652939No Hit
GCCCTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA6870.1497701126872944No Hit
GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT6840.14911609472796122No Hit
GCCCTCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA6640.14475597499907347No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC6530.14235790914818522No Hit
GCCCTCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG6520.14213990316174083No Hit
GCCCTCGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTC6200.1351637115955204No Hit
GCCCTCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA6020.13123960383952143No Hit
GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC6000.13080359186663265No Hit
GCCCTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT5910.12884153798863315No Hit
GCCCTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5700.12426341227330102No Hit
GCCCTCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG5690.12404540628685665No Hit
GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA5510.12012129853085766No Hit
GCCCTCGGGGGGGTTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5480.11946728057152449No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT5310.1157611788019699No Hit
GCCCTCGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCT5280.11510716084263675No Hit
GCCCTCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC5260.11467114886974795No Hit
GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5140.11205507703241531No Hit
GCCCTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA5090.11096504710019336No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT5080.11074704111374899No Hit
GCCCTCGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC5040.10987501716797143No Hit
GCCCTCGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC5030.10965701118152704No Hit
GCCCTCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG4940.10769495730352754No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT4910.1070409393441944No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC4820.1050788854661949No Hit
GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC4790.10442486750686174No Hit
GCCCTCGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC4710.10268081961530663No Hit
GCCCTCGGGCAGCAACCCCCATTCTGGGCCTCTAGGGATGGTACCCCATGCC4690.10224480764241786No Hit
GCCCTCATTGCTCTTTGCCTGTCTCTGCTTTATGCCTGGAAAGCTTTCCAGG4690.10224480764241786No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC4670.10180879566952909No Hit
GCCCTCGGGGGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTTGAG4650.10137278369664032No Hit
GCCCTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT4610.10050075975086277No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA4600.10028275376441836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGA206.3074677E-446.00000418
GAAATCG206.3074677E-446.00000445
CTAGACA206.3074677E-446.00000412
AAATCGC206.3074677E-446.00000446
TCGCAAA206.3074677E-446.00000413
CGAACGT206.3074677E-446.00000416
CTATCGA206.3074677E-446.00000414
CACGCTA206.3074677E-446.00000414
ACCGAAC206.3074677E-446.00000414
AGGGTTA206.3074677E-446.00000433
TTCGTCG206.3074677E-446.00000446
CACGACC206.3074677E-446.00000437
CCTAAGG206.3074677E-446.00000425
ATGTAAG206.3074677E-446.00000424
AACGGAC206.3074677E-446.00000430
TAACCGA206.3074677E-446.00000446
CGCTATA206.3074677E-446.00000416
CGTAGCA405.5988494E-946.00000441
ACGGACT206.3074677E-446.00000425
AGATTTA206.3074677E-446.00000430
Produced by FastQC (version 0.11.5)