FastQCFastQC Report
Fri 17 Jun 2016
SRR1527337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469826
Sequences flagged as poor quality0
Sequence length52
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC60381.2851566324554196No Hit
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG30940.6585416728746387No Hit
GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT28140.598945141392771No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG26770.5697854099177142No Hit
GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC21380.45506208681511884No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATA21360.454636397304534No Hit
GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC20090.42760511338240115No Hit
GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA18400.3916343497379881No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT16600.35332229378535884No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC14150.30117532873872455No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT13910.29606705461170735No Hit
GCCCTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG13510.28755326440001194No Hit
GCCCTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG12930.2752082685930536No Hit
GCCCTCGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA9430.2007126042407189No Hit
GCCCTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG9310.1981584671772103No Hit
GCCCTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG9010.19177312451843873No Hit
GCCCTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8640.1838978685726205No Hit
GCCCTCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT8250.1755969231162175No Hit
GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG7450.15856934269282671No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC7200.1532482238105171No Hit
GCCCTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG7040.14984270772583894No Hit
GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT6530.1389876252059273No Hit
GCCCTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA6490.13813624618475775No Hit
GCCCTCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG6390.13600779863183393No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC6370.13558210912124916No Hit
GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC6320.1345178853447872No Hit
GCCCTCGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTC6190.13175090352598623No Hit
GCCCTCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA6070.1291967664624776No Hit
GCCCTCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA5980.12728116366484613No Hit
GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5960.12685547415426135No Hit
GCCCTCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG5870.1249398713566299No Hit
GCCCTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT5810.1236628028248756No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT5770.12281142380370605No Hit
GCCCTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5660.12047013149548982No Hit
GCCCTCGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC5650.12025728674019744No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT5550.11812883918727358No Hit
GCCCTCGGGGGGGTTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5520.11749030492139642No Hit
GCCCTCGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC5460.11621323638964212No Hit
GCCCTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA5220.1111049622626249No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT5220.1111049622626249No Hit
GCCCTCGGGCAGCAACCCCCATTCTGGGCCTCTAGGGATGGTACCCCATGCC5180.11025358324145534No Hit
GCCCTCGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC5110.10876366995440866No Hit
GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC5100.10855082519911627No Hit
GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA5090.1083379804438239No Hit
GCCCTCGGGGGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTTGAG4980.10599668813560766No Hit
GCCCTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT4970.10578384338031527No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC4940.1051453091144381No Hit
GCCCTCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC4870.10365539582739142No Hit
GCCCTCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG4860.10344255107209903No Hit
GCCCTCGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCT4820.1025911720509295No Hit
GCCCTCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG4760.10131410351917518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA206.307624E-446.00000445
CTACTTT206.307624E-446.00000419
CTCGTGA405.5988494E-946.00000418
CTACTAC206.307624E-446.00000446
CTTTTAC206.307624E-446.00000423
CTATACC206.307624E-446.00000413
AGAAGTT405.5988494E-946.00000415
ATTAATC206.307624E-446.00000428
ATAGATA206.307624E-446.00000419
GTGATAC206.307624E-446.00000419
AGGGTAT206.307624E-446.00000422
TAAAACA206.307624E-446.00000419
AACGGTT206.307624E-446.00000429
TCGTGAT206.307624E-446.00000436
GTGCTAT405.5988494E-946.00000411
CGTAGGA206.307624E-446.00000438
TTAAGCG405.5988494E-946.00000424
CACTACT206.307624E-446.00000439
TGTTACG206.307624E-446.00000440
TTAACGT206.307624E-446.00000423
Produced by FastQC (version 0.11.5)