##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527337_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 469826 Sequences flagged as poor quality 0 Sequence length 52 %GC 63 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.42833942778816 31.0 31.0 34.0 26.0 34.0 2 31.0455338784997 31.0 31.0 34.0 27.0 34.0 3 31.94108670018262 33.0 31.0 34.0 30.0 34.0 4 35.993989264110546 37.0 35.0 37.0 35.0 37.0 5 35.68487695444696 37.0 35.0 37.0 33.0 37.0 6 36.05953693495039 37.0 35.0 37.0 35.0 37.0 7 35.952814446199234 37.0 35.0 37.0 35.0 37.0 8 36.19772000698131 37.0 35.0 37.0 35.0 37.0 9 38.33686939420126 39.0 39.0 39.0 37.0 39.0 10 37.01301971368124 39.0 37.0 39.0 32.0 39.0 11 36.62519954195809 39.0 35.0 39.0 32.0 39.0 12 36.12343931583182 37.0 35.0 39.0 31.0 39.0 13 35.993704052138455 38.0 35.0 39.0 31.0 39.0 14 36.760785907974444 39.0 35.0 40.0 31.0 40.0 15 36.8993606143551 39.0 35.0 40.0 32.0 40.0 16 36.94744862991831 39.0 35.0 40.0 32.0 40.0 17 36.796277770919446 39.0 35.0 40.0 31.0 40.0 18 36.72251429252532 39.0 35.0 40.0 31.0 40.0 19 36.50139626159472 39.0 35.0 40.0 30.0 40.0 20 36.42055995198222 38.0 35.0 40.0 30.0 40.0 21 36.44214666706398 38.0 35.0 40.0 30.0 40.0 22 36.48592244788496 38.0 35.0 40.0 31.0 40.0 23 36.418354880317395 38.0 35.0 40.0 31.0 40.0 24 36.46253293772588 38.0 35.0 40.0 31.0 40.0 25 36.37664156517519 38.0 35.0 40.0 31.0 40.0 26 36.17758489313065 38.0 35.0 40.0 30.0 40.0 27 36.124260896587245 38.0 35.0 40.0 30.0 40.0 28 35.720006981307975 38.0 34.0 40.0 30.0 40.0 29 35.698794872995535 38.0 34.0 40.0 30.0 40.0 30 35.56095448102063 38.0 34.0 40.0 29.0 40.0 31 35.36595250156441 38.0 34.0 40.0 29.0 40.0 32 35.12949687756744 37.0 34.0 40.0 28.0 40.0 33 34.89244954515076 37.0 33.0 39.0 27.0 40.0 34 34.37414915309072 37.0 33.0 39.0 25.0 40.0 35 34.21369187741845 36.0 33.0 39.0 24.0 40.0 36 34.22136067395164 36.0 33.0 39.0 26.0 40.0 37 34.030641130971894 35.0 32.0 39.0 25.0 40.0 38 33.98275744637376 35.0 33.0 39.0 26.0 40.0 39 33.77449098176772 35.0 32.0 39.0 25.0 40.0 40 34.057125403872924 35.0 33.0 39.0 26.0 40.0 41 34.21904492301405 35.0 33.0 39.0 26.0 40.0 42 34.2839051052943 35.0 33.0 39.0 27.0 40.0 43 34.251376041342965 35.0 33.0 39.0 27.0 40.0 44 34.131435893288156 35.0 33.0 39.0 27.0 40.0 45 33.99478104660023 35.0 33.0 39.0 26.0 40.0 46 33.57230762026793 35.0 33.0 38.0 24.0 40.0 47 33.60585408214956 35.0 33.0 38.0 25.0 40.0 48 33.42639189827723 35.0 33.0 37.0 25.0 40.0 49 33.23586604402481 35.0 32.0 37.0 25.0 40.0 50 33.13732317921954 35.0 32.0 37.0 24.0 40.0 51 32.98115898226152 35.0 32.0 37.0 24.0 40.0 52 32.364545597731926 34.0 31.0 36.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 8.0 11 10.0 12 32.0 13 24.0 14 48.0 15 69.0 16 112.0 17 180.0 18 320.0 19 449.0 20 665.0 21 1051.0 22 1436.0 23 2256.0 24 3113.0 25 4241.0 26 5573.0 27 7261.0 28 8605.0 29 10763.0 30 13135.0 31 16578.0 32 21986.0 33 31416.0 34 49176.0 35 50022.0 36 71003.0 37 82340.0 38 80239.0 39 7714.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 98.18188010029245 0.6293819413995819 0.38035357770749173 0.8083843806004777 8 98.27127489751526 0.7909311106665021 0.5676569623647904 0.3701370294534572 9 97.30495970848783 0.9084214155878986 0.5180641343816647 1.2685547415426137 10 67.97942216905834 21.28979664812079 3.8041743113407946 6.926606871480081 11 40.14975756982373 27.02277013192118 14.543469284373364 18.284003013881737 12 34.857798418989155 13.600992707938683 26.699033259121464 24.842175613950694 13 24.938807132853437 16.197060188239902 28.434782238530858 30.429350440375796 14 26.113923026822693 17.19636631433765 29.596914602427283 27.09279605641237 15 27.02277013192118 16.55463937713111 28.445211631540186 27.977378859407526 16 31.85328185328185 17.460080966144915 24.8532435412259 25.833393639347335 17 33.24720215569168 18.321889380323782 22.55771285539753 25.873195608587007 18 31.665978468624555 19.253085184727965 21.539676390834053 27.54125995581343 19 31.5193284322281 19.333540502228487 21.39728324954345 27.749847815999967 20 31.93884544490939 20.755982001847492 20.455445207374645 26.84972734586847 21 33.312758340321736 20.042313537352126 22.632208519749863 24.012719602576276 22 32.803846530417644 16.73342897157671 24.334328027823066 26.12839647018258 23 31.219430172021127 19.309489044880447 21.376637308280085 28.094443474818338 24 33.14099262280078 18.71160812726414 21.809989230055383 26.337410019879698 25 29.09438813518196 18.36573539991401 21.417716346051517 31.12216011885251 26 29.117801058264124 18.455343041892107 23.558508894782324 28.86834700506145 27 30.509805757876318 16.19599596446344 21.578201291541976 31.715996986118267 28 30.720947755126364 22.078599311234374 19.179015209886213 28.021437723753053 29 32.57610264225479 19.731560194625246 20.09807886323873 27.59425829988123 30 30.090927279460907 19.358869028108277 21.519030449570693 29.03117324286012 31 35.59253851425847 18.23355880687744 19.621093766628498 26.55280891223559 32 36.85215377607881 18.62008488248841 19.130699450434843 25.397061890997946 33 35.75004363317483 18.151187886579287 21.301290264906584 24.797478215339297 34 31.60659478189798 20.693831333302114 24.128294304700038 23.571279580099866 35 31.585523151124033 24.273454427809444 21.865541711186694 22.275480709879826 36 31.626602188895465 24.904539127251365 20.0738145611354 23.395044122717774 37 32.63016521009906 24.907944643336045 19.377599366574007 23.08429077999089 38 34.647294956004984 22.505140200840312 20.237705022710536 22.609859820444164 39 35.08085972253558 21.10419602150583 18.038593011029615 25.776351244928975 40 31.07810125450699 20.65317798504127 20.850697917952605 27.41802284249914 41 29.48602248491995 21.17890453061346 21.47944132508631 27.855631659380283 42 27.09790433053939 19.28479905326653 22.635188346323957 30.982108269870125 43 28.374547172783117 17.83532626972539 24.520567188703904 29.269559368787597 44 27.591065628551846 16.647014000928003 23.756667361959536 32.005253008560615 45 29.143768118409795 17.941322957860994 24.315384844602043 28.599524079127164 46 29.001162132363895 20.312839221328748 23.101105515658986 27.584893130648368 47 28.629535189623397 19.051521201466073 23.54020424582718 28.778739363083357 48 31.14514735242409 18.767799142661325 23.706861689221114 26.380191815693472 49 29.482191279324688 18.77801569091536 24.26302503480012 27.47676799495984 50 28.232154031492513 20.182365386334517 22.710322544942173 28.875158037230804 51 26.936780850783055 19.932485643621256 24.47714685862426 28.65358664697143 52 28.64528570151503 19.813079735902228 23.924601873885226 27.617032688697517 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 8.0 10 16.0 11 10.0 12 4.0 13 3.5 14 11.5 15 20.0 16 54.5 17 89.0 18 137.5 19 186.0 20 199.0 21 212.0 22 266.5 23 321.0 24 334.5 25 348.0 26 410.5 27 473.0 28 466.0 29 459.0 30 692.0 31 925.0 32 936.5 33 948.0 34 1210.5 35 1473.0 36 1810.5 37 2148.0 38 2093.0 39 3792.0 40 5546.0 41 4963.5 42 4381.0 43 5257.5 44 6134.0 45 6281.0 46 6428.0 47 6091.0 48 5754.0 49 6932.0 50 8110.0 51 9400.0 52 10690.0 53 13365.5 54 16041.0 55 15013.5 56 13986.0 57 18104.5 58 22223.0 59 23949.5 60 25676.0 61 30992.0 62 36308.0 63 43906.0 64 51077.5 65 50651.0 66 50776.0 67 50901.0 68 44983.5 69 39066.0 70 36648.0 71 34230.0 72 28382.0 73 22534.0 74 19278.0 75 16022.0 76 14275.5 77 12529.0 78 12474.5 79 12420.0 80 9019.0 81 5618.0 82 3794.0 83 1970.0 84 1537.0 85 1104.0 86 655.0 87 206.0 88 151.5 89 59.0 90 21.0 91 16.5 92 12.0 93 6.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 469826.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.799632204262853 #Duplication Level Percentage of deduplicated Percentage of total 1 71.2917387255554 9.125080348895123 2 8.901489956099509 2.2787159501602723 3 3.031462019422642 1.1640479666940529 4 1.4550352534255688 0.7449566435233469 5 0.8547292802979911 0.5470110211014291 6 0.5969801782626047 0.45846760289979693 7 0.4523081016362911 0.4052564140767007 8 0.4190501529865638 0.4290950266694478 9 0.3159505121724092 0.3639645315499781 >10 9.488492749767193 31.946933545610502 >50 2.140148995609951 18.454491662870936 >100 0.9794465877344685 23.495081157705194 >500 0.0515498204070773 4.23220511423378 >1k 0.019954769189836373 5.0695363815540215 >5k 0.0016628974324863642 1.2851566324554196 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 6038 1.2851566324554196 No Hit GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 3094 0.6585416728746387 No Hit GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 2814 0.598945141392771 No Hit GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG 2677 0.5697854099177142 No Hit GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 2138 0.45506208681511884 No Hit GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATA 2136 0.454636397304534 No Hit GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 2009 0.42760511338240115 No Hit GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA 1840 0.3916343497379881 No Hit GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 1660 0.35332229378535884 No Hit GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC 1415 0.30117532873872455 No Hit GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT 1391 0.29606705461170735 No Hit GCCCTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 1351 0.28755326440001194 No Hit GCCCTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG 1293 0.2752082685930536 No Hit GCCCTCGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA 943 0.2007126042407189 No Hit GCCCTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG 931 0.1981584671772103 No Hit GCCCTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG 901 0.19177312451843873 No Hit GCCCTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 864 0.1838978685726205 No Hit GCCCTCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT 825 0.1755969231162175 No Hit GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG 745 0.15856934269282671 No Hit GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 720 0.1532482238105171 No Hit GCCCTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG 704 0.14984270772583894 No Hit GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 653 0.1389876252059273 No Hit GCCCTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA 649 0.13813624618475775 No Hit GCCCTCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG 639 0.13600779863183393 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC 637 0.13558210912124916 No Hit GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC 632 0.1345178853447872 No Hit GCCCTCGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTC 619 0.13175090352598623 No Hit GCCCTCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA 607 0.1291967664624776 No Hit GCCCTCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA 598 0.12728116366484613 No Hit GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC 596 0.12685547415426135 No Hit GCCCTCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG 587 0.1249398713566299 No Hit GCCCTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT 581 0.1236628028248756 No Hit GCCCTCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT 577 0.12281142380370605 No Hit GCCCTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA 566 0.12047013149548982 No Hit GCCCTCGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC 565 0.12025728674019744 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT 555 0.11812883918727358 No Hit GCCCTCGGGGGGGTTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 552 0.11749030492139642 No Hit GCCCTCGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC 546 0.11621323638964212 No Hit GCCCTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA 522 0.1111049622626249 No Hit GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT 522 0.1111049622626249 No Hit GCCCTCGGGCAGCAACCCCCATTCTGGGCCTCTAGGGATGGTACCCCATGCC 518 0.11025358324145534 No Hit GCCCTCGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC 511 0.10876366995440866 No Hit GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC 510 0.10855082519911627 No Hit GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA 509 0.1083379804438239 No Hit GCCCTCGGGGGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTTGAG 498 0.10599668813560766 No Hit GCCCTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT 497 0.10578384338031527 No Hit GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC 494 0.1051453091144381 No Hit GCCCTCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC 487 0.10365539582739142 No Hit GCCCTCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG 486 0.10344255107209903 No Hit GCCCTCGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCT 482 0.1025911720509295 No Hit GCCCTCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG 476 0.10131410351917518 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 2.1284475529238485E-4 0.0 0.0 0.0 0.0 10 0.009578013988157318 0.0 0.0 0.0 0.0 11 0.026605594411548104 0.0 0.0 0.0 0.0 12 0.034906539867951114 0.0 0.0 0.0 0.0 13 0.04959282798312567 0.0 0.0 0.0 0.0 14 0.06640756365122406 0.0 0.0 0.0 0.0 15 0.07939109372405954 0.0 0.0 0.0 0.0 16 0.08918195246750925 0.0 0.0 0.0 0.0 17 0.10024987974271327 0.0 0.0 0.0 0.0 18 0.10833798044382388 0.0 0.0 0.0 0.0 19 0.11642608114493451 0.0 0.0 0.0 0.0 20 0.12408849233546036 0.0 0.0 0.0 0.0 21 0.1306866797495243 0.0 0.0 0.0 0.0 22 0.13558210912124916 0.0 0.0 0.0 0.0 23 0.14388305457765216 0.0 0.0 0.0 0.0 24 0.1530353790552247 0.0 0.0 0.0 0.0 25 0.16069779024575057 0.0 0.0 0.0 0.0 26 0.16899873570215357 0.0 0.0 0.0 0.0 27 0.18198226577498905 0.0 0.0 0.0 0.0 28 0.27052568397662113 0.0 0.0 0.0 0.0 29 0.28287067978357944 0.0 0.0 0.0 0.0 30 0.3116047217480514 0.0 0.0 0.0 0.0 31 0.32203411475737825 0.0 0.0 0.0 0.0 32 0.33182497350082796 0.0 0.0 0.0 0.0 33 0.33757178189372233 0.0 0.0 0.0 0.0 34 0.34651126161600254 0.0 0.0 0.0 0.0 35 0.3812049567286612 0.0 0.0 0.0 0.0 36 0.395465555333251 0.0 0.0 0.0 0.0 37 0.4056821035872855 0.0 0.0 0.0 0.0 38 0.413131670022519 0.0 0.0 0.0 0.0 39 0.4197298574365829 0.0 0.0 0.0 0.0 40 0.4280308028929859 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCGTA 20 6.307624E-4 46.000004 45 CTACTTT 20 6.307624E-4 46.000004 19 CTCGTGA 40 5.5988494E-9 46.000004 18 CTACTAC 20 6.307624E-4 46.000004 46 CTTTTAC 20 6.307624E-4 46.000004 23 CTATACC 20 6.307624E-4 46.000004 13 AGAAGTT 40 5.5988494E-9 46.000004 15 ATTAATC 20 6.307624E-4 46.000004 28 ATAGATA 20 6.307624E-4 46.000004 19 GTGATAC 20 6.307624E-4 46.000004 19 AGGGTAT 20 6.307624E-4 46.000004 22 TAAAACA 20 6.307624E-4 46.000004 19 AACGGTT 20 6.307624E-4 46.000004 29 TCGTGAT 20 6.307624E-4 46.000004 36 GTGCTAT 40 5.5988494E-9 46.000004 11 CGTAGGA 20 6.307624E-4 46.000004 38 TTAAGCG 40 5.5988494E-9 46.000004 24 CACTACT 20 6.307624E-4 46.000004 39 TGTTACG 20 6.307624E-4 46.000004 40 TTAACGT 20 6.307624E-4 46.000004 23 >>END_MODULE