FastQCFastQC Report
Fri 17 Jun 2016
SRR1527336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13183
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGCTGTCCTGGAACTCTCAAGAGATTTTCCTGCCTCTGCCTC4363.307289691269059No Hit
AAGCGGGGGAGTCTTGGGAACCATGCAGTTCCAGAGGGACGTCCCCTCCC3322.5183949025259804No Hit
AAGCGGGGGGGGAAAGAGAACGTGAACGAGAAAAGGAAAAAGAACGGGAG3242.4577106880072823No Hit
AAGCGGGGGGCTCCTAACCGACAAGCGTCTGTGAGGGGAGCGTCCAGGTC3082.3363422589698852No Hit
AAGCGGGGATGAGAACAACCCACGTGGGCATGGCCACAGTCATGGCAAAG3012.2832435712660244No Hit
AAGCGGGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGG2521.9115527573389972No Hit
AAGCGGCTCACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAA2491.8887961768944854No Hit
AAGCGGGGCACAAGAACTAGCCAGGGTCCCTTTGCTTTCCTGTGTTCAAG1851.4033224607448986No Hit
AAGCGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT1771.3426382462262003No Hit
AAGCGGGGGACCCTGGAAACCACAGAGAGCGGCTGCTGAGCCCAAGAGGA1731.3122961389668513No Hit
AAGCGGAGCAGTCCAAATGTTTTTGAGATGGCAGCAACGGAAACCATAAC1651.251611924448153No Hit
AAGCGGGGGAGCCCTTTGCCGCGAATAGCCGGAAGAAAATACATCTTTTG1651.251611924448153No Hit
AAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGC1651.251611924448153No Hit
AAGCGGGGGGGGAACCAGTGACTGGGCACGCATGTGGGGGTTACTCACAG1611.2212698171888037No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC1551.17575665629978No Hit
AAGCGGTCTGGAAACGTACGGATTGTTCAGTAACTTGACTCATGATTTCT1441.09231586133657No Hit
AAGCGGGGGAGCTCGGAGTGTAACCCTCTGTGACGGTCGCTGTCTCAGGA1381.0468027004475462No Hit
AAGCGGGGGATCAGATTGGCAGCTAAACAGTTGTATTAGATAATCTTCAA1381.0468027004475462No Hit
AAGCGGGGGATGTGGGAACTGAACCAGGGTCCTTTTAGGAAAGCAACAAG1341.016460593188197No Hit
AAGCGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC1260.9557763786694986No Hit
AAGCGGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG1230.9330197982249867No Hit
AAGCGGGGGAGTCTGAAACCATCTGAGCAGAGTCTCTCTTCAGTCCCCGG1140.8647500568914511No Hit
AAGCGGGGGACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGG1080.8192368960024274No Hit
AAGCGGGGGAACTACTGTATGGAGAGGAGCGGTGTTGGGAAATGTGAACC1070.8116513691875901No Hit
AAGCGGGGAACACACACACACATACATACACCCAACAACAACAGCAACAA970.7357961010392172No Hit
AAGCGGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA970.7357961010392172No Hit
AAGCGGGGGATAATAGATTCTGCCAGCTGTTAGCTGTTCAGTTCATAAGC950.7206250474095426No Hit
AAGCGGGGGAGAGCCACCAGCCATGCCCCAGCCAGTGCCTACAGCATAAC940.7130395205947053No Hit
AAGCGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAA930.7054539937798681No Hit
AAGCGGGGGGGGAGCTGACATCACACATGGGTGGCATATGTGGGATGTAG930.7054539937798681No Hit
AAGCGGGGGAGAATTCTCAAGGTCTGTTTTTTGTTGTTTTGTTTTTGTTT880.6675263597056815No Hit
AAGCGGCATGGTCAATATAACCAGTAGTGTTAACAGTCGGGAGAGGAGTG840.6371842524463325No Hit
AAGCGGGGGAAGCGAGGCACGAGTCCACGGCCTCCTGAGGGCGGCCTGGG840.6371842524463325No Hit
AAGCGGGGGGTCTGGAATTTGATGAAGTTAAAGTCAAGCAAATCCTGAAG810.6144276720018206No Hit
AAGCGGGGGAGGCGCCTTTGAGGACTCCCTGTGAGCCAGGCAGTATCGCA780.5916710915573087No Hit
AAGCGGGGACGCACTGACAGGCCTCAAGACCCCAAGCTCTGAGAGAAGGG780.5916710915573087No Hit
AAGCGGGGGAACAGCAAGCTGGCGCCTGCGCTCTCTGTCAAGGCGGCCCA760.576500037927634No Hit
AAGCGGGGGGCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGCAGTG730.5537434574831221No Hit
AAGCGGGGGACTTCAACAAAAAGAGAGAAAGAACTATGCTAAGGACATAT730.5537434574831221No Hit
AAGCGGGGGACCCTGAGCAAGTAGGAATTCAGACAAATGGTGGAAGCACA720.546157930668285No Hit
AAGCGGGGGAGAGGAAGCGAAGGATAGCTTTGTATTAATGTTGCGTTAAA710.5385724038534476No Hit
AAGCGGGGGGCTGATGGCAAAGTTGATCTTTGCCTGTCATGAGAAGCTGC710.5385724038534476No Hit
AAGCGGGGGAAGATAGCCACCCGGAAGGCCTATGGACTGGCCCTCGCTAA690.5234013502237731No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC680.5158158234089357No Hit
AAGCGGGGGGAGCAGTGTGAGGAAGCAGCGAGGACGACCCCCGGACGGAG640.48547371614958656No Hit
AAGCGGGGGGAAGGTGTATGAGTTTCGACCCCAGAGCTCTGTGTGCTTTC620.470302662519912No Hit
AAGCGGGGGGCTGGCCGAGCAGGAGGCGCCATCATGGGTGTTGACATTCG620.470302662519912No Hit
AAGCGGGGGATCGACACCTTCCATCAATACTCTAGGAAGGAAGGACACCC620.470302662519912No Hit
AAGCGGGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGAT610.46271713570507467No Hit
AAGCGGGGGGGCCATGGGTGGAAGTGTGTAGGTTTTATGCAAATATTTTG600.4551316088902374No Hit
AAGCGGGGGGCGCAAGGTGCGCGGGTCCGGTTATGAAGCTTGGCCGGGCC590.4475460820754002No Hit
AAGCGGGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTAGG580.4399605552605629No Hit
AAGCGGGGGCCCCTTTCTGCGCAGGAAGGCTGAGGTGCTAGCACCAGCCT570.43237502844572556No Hit
AAGCGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACC570.43237502844572556No Hit
AAGCGGGGGGTCATTACCTGATTCTGTCGTGAGTGGGTACTTTGGCTCAT570.43237502844572556No Hit
AAGCGGCATGGTCAATATAACCAGCAGTGTTAACAGTCGGGAGAGGAGTG560.4247895016308883No Hit
AAGCGGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA550.417203974816051No Hit
AAGCGGGGGACTCCAGACATGCTCTGAGTGTCACGTCCGCCCTGGATCTA550.417203974816051No Hit
AAGCGGGGGGTTTGAAAGGAAATCTTTCGTGACAATGCCGTCTGAACTGG540.4096184480012137No Hit
AAGCGGGGGGGTCTTGGCGGGCTGGCAGCAGGCAGGCTGCAGCAGGCGAG540.4096184480012137No Hit
AAGCGGGGAGATAGGCACAGCATCAAGGCAAGACACAGTAGTGCTCCCTG530.4020329211863764No Hit
AAGCGGGGGAGACAAGCACACGTTGACCACGTGTGGAAAAATAAAGTTGC530.4020329211863764No Hit
AAGCGGGGGATGACAACTCACTCAAGATTGTCAGCAATGCATCCTGCACC520.3944473943715391No Hit
AAGCGGGGGGCTGGGGTACAGTGGATGTGAATACTGTAAATAGCTTGTGC520.3944473943715391No Hit
AAGCGGGGGTTCCTGTTTGCAGGCTTAACTCCGGCCGGGGTTCCTCGCTC510.3868618675567018No Hit
AAGCGGGGGGCCGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTG510.3868618675567018No Hit
AAGCGGGGGGAGTGTGGTGCAGTGGGTGCAGTGCTATGCAGTGCACGCAG490.37169081392702724No Hit
AAGCGGGGGGATCTCTCTCGCAGCACTGCCACAGGTGCTAGTAGGGCCAA490.37169081392702724No Hit
AAGCGGGGGACCTTAACTTGGTGCTACCCTGTCTGGAGTACCACAACAAC460.34893423348251534No Hit
AAGCGGGGGGAGATGGGTAGTGGAGGTAAAGAGGAGGTCATAGTATGCAG460.34893423348251534No Hit
AAGCGGGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCT460.34893423348251534No Hit
AAGCGGGGGGTGGTTTTTTTTTTCCAGTTTTGGGGGTTTTTTGTTTTGTT450.3413487066676781No Hit
AAGCGGGGCATACACTGTGTCCCCGGTGTAATGTTTGTAACTGATGTCTG450.3413487066676781No Hit
AAGCGGGGGACCGGAACTTGGTAGCTCTGAGAGACATGGGACTGACGCTC440.33376317985284076No Hit
AAGCGGGTATCGGAGGCAGCTATATATAAGAGGGGAGGTATCCCACTAGG440.33376317985284076No Hit
AAGCGGGGGGGAGCAGTGTGAGGAAGCAGCGAGGACGACCCCCGGACGGA430.32617765303800345No Hit
AAGCGGGGGAGAAAACCATCCTGAGACTCCTAAAAAAAAGTCTGATCCTG430.32617765303800345No Hit
AAGCGGGGGTAGCACTGGGCATTTCGCCTAACAATACTGTGACTCAGTAT430.32617765303800345No Hit
AAGCGGGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCT420.31859212622316624No Hit
AAGCGGGGAACTCAGAGGGTTGATACTTTTAATTTGTAATTTTTTGTAAA400.3034210725934916No Hit
AAGCGGGGGGGACATTGGCGTCTTTGCTAAGGGTCACATTGAAGTAACGC390.29583554577865434No Hit
AAGCGGGGGGAAGTTGAAGAAAACAGAAACGCAAGAGAAAAATCCTCTGC390.29583554577865434No Hit
AAGCGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGA380.288250018963817No Hit
AAGCGGGGGAGACGGCGGGACAGACGGCTGGGTGTGCAGCGACCTCGAAC380.288250018963817No Hit
AAGCGGGGAACCACTTGTAGCTTATTTCCATCATAATTTGATGATAGATT370.2806644921489797No Hit
AAGCGGGGGATGAGTGTGATTTTGATCTGTTTTCCAAGTGCACCCAAAGT370.2806644921489797No Hit
AAGCGGCAAAGCCTGAAGGTCTCCACTGAGGACTGTGAGCGAGAGCAGCT370.2806644921489797No Hit
AAGCGGGGGGGCTATCTGCTGCCTGGCGCTGGCTGACGGATTGCTGCAGC370.2806644921489797No Hit
AAGCGGGGGAGATGGAGCGCAGCATAACCACCATCATCGACACCTTCCAT370.2806644921489797No Hit
AAGCGGGGGATCAGGCAAGACCCATGAGGCAGAGATCGTAGAAGGAGAGA360.2730789653341425No Hit
AAGCGGGGGGAGTGTGACACCCTTGTAAGAGAGCTAGAGCAAGATTCGGA360.2730789653341425No Hit
AAGCGGGGGATACAATGGCGCTATTAATGTCAGCTTTTCAAAATACAGGC360.2730789653341425No Hit
AAGCGGGGGAAACCAGCCTCAGTTTCAGAGGAAGAGCTGCTGAATTCAAT360.2730789653341425No Hit
AAGCGGGGGATAACGGAAAACGACCACGTTCTCCTCTTTTGGAAAAGCCT360.2730789653341425No Hit
AAGCGGGGGACCTTATGGATCTGAGCCACCTCATCCTGGACAAGGCCTCT360.2730789653341425No Hit
AAGCGGGGGATTAGCCAGATTGTGTCTTCCATCACTGCTTCCCTCAGATT350.2654934385193052No Hit
AAGCGGGGGGTCAGCGACCCAGCACAAAAGCTTTGTCTCCAGACCCTCTC350.2654934385193052No Hit
AAGCGGGGGTCTTCCGGTCGGGCCGAGCTCGTCGCTAGGTTTGTTGAAGG350.2654934385193052No Hit
AAGCGGGGGATTATATCACATTTCCAAAAAGTAGTACCATGAAGATGGCG350.2654934385193052No Hit
AAGCGGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTT350.2654934385193052No Hit
AAGCGGGGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCA340.25790791170446786No Hit
AAGCGGGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGG340.25790791170446786No Hit
AAGCGGGGGATGTGCAGCTGCCAGCAGATGAGGTGGACACACAACTGCTA340.25790791170446786No Hit
AAGCGGGGGGAACAGACACCATGATCATCGCAACTCTTGGTCATGTTCTT330.2503223848896306No Hit
AAGCGGGGGTTTGGTGGAGTGATGATGTGGTCTCAGTAGGAACGGTAACA330.2503223848896306No Hit
AAGCGGGGGGCAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCC330.2503223848896306No Hit
AAGCGGGGGGAAGGTTGAATATTGAGTGTCCCGGGAGGTCAGATTGCTGT320.24273685807479328No Hit
AAGCGGGGGGAGCTGACCGGCGGCCCTTACATCCCCCTGGGTGGTGACTT320.24273685807479328No Hit
AAGCGGGGGCAGCATGGAAGGTCCAGCGTTCTCAAAATCCCTTAAAGATA320.24273685807479328No Hit
AAGCGGGGGATGTTTGGAAAGCCTTTGAAAGGGCAGTAAGCATGTGGTGA320.24273685807479328No Hit
AAGCGGGGGGCTGGCAAAGAAAAGCAAAGAAGTATTCAGAAAAGAGATGT290.21998027763028144No Hit
AAGCGGGGGGAGTCTTGGGAACCATGCAGTTCCAGAGGGACGTCCCCTCC280.21239475081544415No Hit
AAGCGGGGGAGCCTATGGAGGTGTTTGTGGACGACGAGACCAAGCTCACA280.21239475081544415No Hit
AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCT270.20480922400060686No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG260.19722369718576954No Hit
AAGCGGGGGGGGGCTTCCTGCCCAGAGCTTCAACCCTGCCAAGGCGGAGG260.19722369718576954No Hit
AAGCGGGGGATGCACTGTACATTCCACGAGCATTGCCTTCTTATTTTACT260.19722369718576954No Hit
AAGCGGGGGATCCCAGGCTCAGTGCCTTCCTGCCACCTGCTGCAGGTATA250.18963817037093225No Hit
AAGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC250.18963817037093225No Hit
AAGCGGGGGGCTCGGTTGGTACGTGCTTAGTGCTGGTATGGCTTCCAGCG250.18963817037093225No Hit
AAGCGGGGGACCCTTTGTGGGGGGAAAATGAGCCTTTGGACATAGACAGT240.182052643556095No Hit
AAGCGGGGGGGGAGATCGCGGACGGGCTGTGCCTGGAGGTGGAGGGCAAG230.17446711674125767No Hit
AAGCGGGGGAAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGG230.17446711674125767No Hit
AAGCGGGGGGATTCAGAGGGTACAGAACAACCTCTATTATTTCAGAGTAC220.16688158992642038No Hit
AAGCGGGGGCATTTTTGCTGGTTTAAAAAAAGATTTCTTTATGCCTATAT220.16688158992642038No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG220.16688158992642038No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT220.16688158992642038No Hit
AAGCGGGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACCCGACTG210.15929606311158312No Hit
AAGCGGGGGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAACAAAGCA210.15929606311158312No Hit
AAGCGGGGGATCAGATTAGCAGCTAAACAGTTGTATTAGATAATCTTCAA210.15929606311158312No Hit
AAGCGGGGGGGGTGATCCATTCATGCTGCAGCAGTCTACAAACCCAGCAC190.1441250094819085No Hit
AAGCGGGGGACTGAAGTGATGGCGGCGGCCGAGCGGGCATGGGGAGCAGC180.13653948266707125No Hit
AAGCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT170.12895395585223393No Hit
AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC160.12136842903739664No Hit
AAGCGGGGGGACTTTCCATTGCTCTGCATGTAGGTTTTGACCACTTCGAG160.12136842903739664No Hit
AAGCGGGGGATCTGTTTGGGGCATCTTTGGGAGGCTTTTTGGCTCAAAAA160.12136842903739664No Hit
AAGCGGGGGAGCACCCCGTGGGGTCCCTCAGATTGAGGTTACTTTTGACA150.11378290222255935No Hit
AAGCGGGGGGAAGGTATAGGGAACTTTCGGGATAGCATGTGAAATGTGAA150.11378290222255935No Hit
AAGCGGGGGATTCCTATATTTGGAAAGAGTACAGTTTCTAACGGCAGCAG150.11378290222255935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAC302.3415723E-644.00000410
ACAGTCA302.3415723E-644.00000436
AGCCCAA302.3415723E-644.00000439
CCAACCA302.3415723E-644.00000434
TGGCAAA302.3415723E-644.00000443
AACAACC302.3415723E-644.00000414
CAGTCAT302.3415723E-644.00000437
CTGAGCC302.3415723E-644.00000436
CACGTGG302.3415723E-644.00000421
CAACCCA302.3415723E-644.00000416
CAACCAG302.3415723E-644.00000435
CAACCAA302.3415723E-644.00000431
GGCAAAG302.3415723E-644.00000444
GCGGCTC302.3415723E-644.0000043
CACAACC302.3415723E-644.00000429
ATGGCAA302.3415723E-644.00000442
CCAGAAC302.3415723E-644.00000438
GAGCCCA302.3415723E-644.00000438
CAACAGG302.3415723E-644.00000421
AACCCAC302.3415723E-644.00000417
Produced by FastQC (version 0.11.5)