FastQCFastQC Report
Fri 17 Jun 2016
SRR1527335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10945
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGCTGTCCTGGAACTCTCAAGAGATTTTCCTGCCTCTGCCTCTG3943.5998172681589766No Hit
AAGCGGGGGGGGAAAGAGAACGTGAACGAGAAAAGGAAAAAGAACGGGAGCG2972.7135678391959797No Hit
AAGCGGGGGAGTCTTGGGAACCATGCAGTTCCAGAGGGACGTCCCCTCCCTG2842.594792142530836No Hit
AAGCGGGGATGAGAACAACCCACGTGGGCATGGCCACAGTCATGGCAAAGGC2492.2750114207400642No Hit
AAGCGGGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG2272.0740063956144357No Hit
AAGCGGGGGGCTCCTAACCGACAAGCGTCTGTGAGGGGAGCGTCCAGGTCCC2262.064869803563271No Hit
AAGCGGCTCACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAA1761.6080402010050252No Hit
AAGCGGGGCACAAGAACTAGCCAGGGTCCCTTTGCTTTCCTGTGTTCAAGCC1691.5440840566468708No Hit
AAGCGGGGGATCAGATTGGCAGCTAAACAGTTGTATTAGATAATCTTCAAAT1481.3522156235724074No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC1461.3339424394700776No Hit
AAGCGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG1391.2699862951119234No Hit
AAGCGGGGGAGCTCGGAGTGTAACCCTCTGTGACGGTCGCTGTCTCAGGAAT1371.2517131110095936No Hit
AAGCGGAGCAGTCCAAATGTTTTTGAGATGGCAGCAACGGAAACCATAACGA1361.2425765189584286No Hit
AAGCGGTCTGGAAACGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTA1341.2243033348560985No Hit
AAGCGGGGGGGGAACCAGTGACTGGGCACGCATGTGGGGGTTACTCACAGGA1341.2243033348560985No Hit
AAGCGGGGGACCCTGGAAACCACAGAGAGCGGCTGCTGAGCCCAAGAGGACC1301.187756966651439No Hit
AAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGC1221.1146642302421197No Hit
AAGCGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT1151.0507080858839652No Hit
AAGCGGGGGAGCCCTTTGCCGCGAATAGCCGGAAGAAAATACATCTTTTGCA1131.0324349017816354No Hit
AAGCGGGGGAGTCTGAAACCATCTGAGCAGAGTCTCTCTTCAGTCCCCGGGA1050.9593421653723162No Hit
AAGCGGGGAACACACACACACATACATACACCCAACAACAACAGCAACAATA1040.9502055733211513No Hit
AAGCGGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA950.8679762448606669No Hit
AAGCGGGGGAGAATTCTCAAGGTCTGTTTTTTGTTGTTTTGTTTTTGTTTTT940.858839652809502No Hit
AAGCGGGGGAACTACTGTATGGAGAGGAGCGGTGTTGGGAAATGTGAACCCT880.8040201005025126No Hit
AAGCGGGGGGGGAGCTGACATCACACATGGGTGGCATATGTGGGATGTAGGG870.7948835084513476No Hit
AAGCGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATT830.758337140246688No Hit
AAGCGGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA810.7400639561443582No Hit
AAGCGGGGGACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCC790.7217907720420284No Hit
AAGCGGGGGATAATAGATTCTGCCAGCTGTTAGCTGTTCAGTTCATAAGCTT780.7126541799908634No Hit
AAGCGGGGGGAAGGTGTATGAGTTTCGACCCCAGAGCTCTGTGTGCTTTCAT770.7035175879396985No Hit
AAGCGGGGGATGTGGGAACTGAACCAGGGTCCTTTTAGGAAAGCAACAAGGG750.6852444038373686No Hit
AAGCGGGGGAGAGCCACCAGCCATGCCCCAGCCAGTGCCTACAGCATAACAG700.6395614435815441No Hit
AAGCGGGGGAAGCGAGGCACGAGTCCACGGCCTCCTGAGGGCGGCCTGGGCT680.6212882594792143No Hit
AAGCGGGGGACTTCAACAAAAAGAGAGAAAGAACTATGCTAAGGACATATAT670.6121516674280493No Hit
AAGCGGGGACGCACTGACAGGCCTCAAGACCCCAAGCTCTGAGAGAAGGGCT660.6030150753768844No Hit
AAGCGGGGGAGAGGAAGCGAAGGATAGCTTTGTATTAATGTTGCGTTAAAGC660.6030150753768844No Hit
AAGCGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAG650.5938784833257195No Hit
AAGCGGGGGGTCTGGAATTTGATGAAGTTAAAGTCAAGCAAATCCTGAAGAA630.5756052992233897No Hit
AAGCGGGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTAGGTC620.5664687071722248No Hit
AAGCGGGGGTTCCTGTTTGCAGGCTTAACTCCGGCCGGGGTTCCTCGCTCGA620.5664687071722248No Hit
AAGCGGCATGGTCAATATAACCAGTAGTGTTAACAGTCGGGAGAGGAGTGGC610.5573321151210598No Hit
AAGCGGGGGAAGATAGCCACCCGGAAGGCCTATGGACTGGCCCTCGCTAAGC600.5481955230698949No Hit
AAGCGGGGGACCCTGAGCAAGTAGGAATTCAGACAAATGGTGGAAGCACAGT580.529922338967565No Hit
AAGCGGGGGAACAGCAAGCTGGCGCCTGCGCTCTCTGTCAAGGCGGCCCAGA580.529922338967565No Hit
AAGCGGGGGATCGACACCTTCCATCAATACTCTAGGAAGGAAGGACACCCTG560.5116491548652352No Hit
AAGCGGGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGC560.5116491548652352No Hit
AAGCGGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT550.5025125628140703No Hit
AAGCGGGGGGCTGATGGCAAAGTTGATCTTTGCCTGTCATGAGAAGCTGCAT540.49337597076290546No Hit
AAGCGGGGGGGCCATGGGTGGAAGTGTGTAGGTTTTATGCAAATATTTTGCT540.49337597076290546No Hit
AAGCGGGGGGAGCAGTGTGAGGAAGCAGCGAGGACGACCCCCGGACGGAGCA540.49337597076290546No Hit
AAGCGGGGGACTCCAGACATGCTCTGAGTGTCACGTCCGCCCTGGATCTACT540.49337597076290546No Hit
AAGCGGGGGGCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGCAGTGTG530.48423937871174055No Hit
AAGCGGCATGGTCAATATAACCAGCAGTGTTAACAGTCGGGAGAGGAGTGGC520.47510278666057565No Hit
AAGCGGGGGAGACAAGCACACGTTGACCACGTGTGGAAAAATAAAGTTGCTG510.46596619460941063No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC500.45682960255824573No Hit
AAGCGGGGGTCTTCCGGTCGGGCCGAGCTCGTCGCTAGGTTTGTTGAAGGAC500.45682960255824573No Hit
AAGCGGGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCG490.4476930105070808No Hit
AAGCGGGGGAGAAAACCATCCTGAGACTCCTAAAAAAAAGTCTGATCCTGAG480.4385564184559159No Hit
AAGCGGGGGGCGCAAGGTGCGCGGGTCCGGTTATGAAGCTTGGCCGGGCCGC470.429419826404751No Hit
AAGCGGGTATCGGAGGCAGCTATATATAAGAGGGGAGGTATCCCACTAGGTG460.4202832343535861No Hit
AAGCGGGGGGCTGGCCGAGCAGGAGGCGCCATCATGGGTGTTGACATTCGCC460.4202832343535861No Hit
AAGCGGGGGGAGTGTGGTGCAGTGGGTGCAGTGCTATGCAGTGCACGCAGTG450.4111466423024212No Hit
AAGCGGGGGAGGCGCCTTTGAGGACTCCCTGTGAGCCAGGCAGTATCGCAGT450.4111466423024212No Hit
AAGCGGGGGGAGATGGGTAGTGGAGGTAAAGAGGAGGTCATAGTATGCAGGG440.4020100502512563No Hit
AAGCGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA430.39287345820009134No Hit
AAGCGGGGGGTGGTTTTTTTTTTCCAGTTTTGGGGGTTTTTTGTTTTGTTTT410.37460027409776153No Hit
AAGCGGGGGGGACATTGGCGTCTTTGCTAAGGGTCACATTGAAGTAACGCTA400.3654636820465966No Hit
AAGCGGGGGAGATGGAGCGCAGCATAACCACCATCATCGACACCTTCCATCA380.3471904979442668No Hit
AAGCGGGGGCCCCTTTCTGCGCAGGAAGGCTGAGGTGCTAGCACCAGCCTGG380.3471904979442668No Hit
AAGCGGGGGAGACGGCGGGACAGACGGCTGGGTGTGCAGCGACCTCGAACCC380.3471904979442668No Hit
AAGCGGGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCTCC370.33805390589310186No Hit
AAGCGGGGGGAGTGTGACACCCTTGTAAGAGAGCTAGAGCAAGATTCGGAGA370.33805390589310186No Hit
AAGCGGGGGGCTGGCAAAGAAAAGCAAAGAAGTATTCAGAAAAGAGATGTCT370.33805390589310186No Hit
AAGCGGGGGGCCGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA360.32891731384193695No Hit
AAGCGGGGGGTTTGAAAGGAAATCTTTCGTGACAATGCCGTCTGAACTGGAG360.32891731384193695No Hit
AAGCGGGGGGGTCTTGGCGGGCTGGCAGCAGGCAGGCTGCAGCAGGCGAGCA350.31978072179077205No Hit
AAGCGGGGGGGCTATCTGCTGCCTGGCGCTGGCTGACGGATTGCTGCAGCAC350.31978072179077205No Hit
AAGCGGGGGTTTGGTGGAGTGATGATGTGGTCTCAGTAGGAACGGTAACATT350.31978072179077205No Hit
AAGCGGGGGGTCAGCGACCCAGCACAAAAGCTTTGTCTCCAGACCCTCTCTT350.31978072179077205No Hit
AAGCGGGGGATACAATGGCGCTATTAATGTCAGCTTTTCAAAATACAGGCTT340.31064412973960714No Hit
AAGCGGGGGATTATATCACATTTCCAAAAAGTAGTACCATGAAGATGGCGAG330.3015075376884422No Hit
AAGCGGGGAACTCAGAGGGTTGATACTTTTAATTTGTAATTTTTTGTAAAAC330.3015075376884422No Hit
AAGCGGGGGGAAGTTGAAGAAAACAGAAACGCAAGAGAAAAATCCTCTGCCT330.3015075376884422No Hit
AAGCGGGGGACCTTAACTTGGTGCTACCCTGTCTGGAGTACCACAACAACAC320.2923709456372773No Hit
AAGCGGGGGGCTGGGGTACAGTGGATGTGAATACTGTAAATAGCTTGTGCTA320.2923709456372773No Hit
AAGCGGGGGGATCTCTCTCGCAGCACTGCCACAGGTGCTAGTAGGGCCAATT310.2832343535861124No Hit
AAGCGGGGGACCCTTTGTGGGGGGAAAATGAGCCTTTGGACATAGACAGTAA310.2832343535861124No Hit
AAGCGGGGGAGCCTATGGAGGTGTTTGTGGACGACGAGACCAAGCTCACACT310.2832343535861124No Hit
AAGCGGGGAACCACTTGTAGCTTATTTCCATCATAATTTGATGATAGATTGA310.2832343535861124No Hit
AAGCGGGGGATAACGGAAAACGACCACGTTCTCCTCTTTTGGAAAAGCCTAG310.2832343535861124No Hit
AAGCGGGGGACCGGAACTTGGTAGCTCTGAGAGACATGGGACTGACGCTCCG300.27409776153494747No Hit
AAGCGGGGGATGCACTGTACATTCCACGAGCATTGCCTTCTTATTTTACTTC300.27409776153494747No Hit
AAGCGGGGAGATAGGCACAGCATCAAGGCAAGACACAGTAGTGCTCCCTGCT300.27409776153494747No Hit
AAGCGGGGGATGAGTGTGATTTTGATCTGTTTTCCAAGTGCACCCAAAGTCT300.27409776153494747No Hit
AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCT290.2649611694837825No Hit
AAGCGGGGGAAACCAGCCTCAGTTTCAGAGGAAGAGCTGCTGAATTCAATCA290.2649611694837825No Hit
AAGCGGGGGGAGTCTTGGGAACCATGCAGTTCCAGAGGGACGTCCCCTCCCT290.2649611694837825No Hit
AAGCGGGGGGAGCTGACCGGCGGCCCTTACATCCCCCTGGGTGGTGACTTCG280.2558245774326176No Hit
AAGCGGGGGACCTTATGGATCTGAGCCACCTCATCCTGGACAAGGCCTCTGG280.2558245774326176No Hit
AAGCGGGGGATTAGCCAGATTGTGTCTTCCATCACTGCTTCCCTCAGATTTG280.2558245774326176No Hit
AAGCGGGGCATACACTGTGTCCCCGGTGTAATGTTTGTAACTGATGTCTGTT280.2558245774326176No Hit
AAGCGGCAAAGCCTGAAGGTCTCCACTGAGGACTGTGAGCGAGAGCAGCTGA280.2558245774326176No Hit
AAGCGGGGGAAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC270.24668798538145273No Hit
AAGCGGGGGATGACAACTCACTCAAGATTGTCAGCAATGCATCCTGCACCAC270.24668798538145273No Hit
AAGCGGGGGGTCATTACCTGATTCTGTCGTGAGTGGGTACTTTGGCTCATTT270.24668798538145273No Hit
AAGCGGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTTCA270.24668798538145273No Hit
AAGCGGGGGATGTGCAGCTGCCAGCAGATGAGGTGGACACACAACTGCTACA260.23755139333028782No Hit
AAGCGGGGGGAAGGTTGAATATTGAGTGTCCCGGGAGGTCAGATTGCTGTCA250.22841480127912286No Hit
AAGCGGGGGATGTTTGGAAAGCCTTTGAAAGGGCAGTAAGCATGTGGTGATA240.21927820922795796No Hit
AAGCGGGGGGCAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAA240.21927820922795796No Hit
AAGCGGGGGAGCACCCCGTGGGGTCCCTCAGATTGAGGTTACTTTTGACATC230.21014161717679306No Hit
AAGCGGGGGGGGAGATCGCGGACGGGCTGTGCCTGGAGGTGGAGGGCAAGAT230.21014161717679306No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT220.20100502512562815No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT220.20100502512562815No Hit
AAGCGGGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACCCGACTGTT220.20100502512562815No Hit
AAGCGGGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC210.19186843307446325No Hit
AAGCGGGGGATCCCAGGCTCAGTGCCTTCCTGCCACCTGCTGCAGGTATAGG210.19186843307446325No Hit
AAGCGGGGGGTCCGAGTAACCGCCAAGATGGCCGAAGTGGAGCAGAAGAAGA200.1827318410232983No Hit
AAGCGGGGGTAGCACTGGGCATTTCGCCTAACAATACTGTGACTCAGTATTC200.1827318410232983No Hit
AAGCGGGGGGGAGCAGTGTGAGGAAGCAGCGAGGACGACCCCCGGACGGAGC200.1827318410232983No Hit
AAGCGGGGGATCAGGCAAGACCCATGAGGCAGAGATCGTAGAAGGAGAGAAC200.1827318410232983No Hit
AAGCGGGGGGCTCGGTTGGTACGTGCTTAGTGCTGGTATGGCTTCCAGCGGA200.1827318410232983No Hit
AAGCGGGGGCATTTTTGCTGGTTTAAAAAAAGATTTCTTTATGCCTATATGT200.1827318410232983No Hit
AAGCGGGGGCAGCATGGAAGGTCCAGCGTTCTCAAAATCCCTTAAAGATAAG190.1735952489721334No Hit
AAGCGGGGGAGGCCTTTGCCACCAACCCCTCTGCTTGCTTGAGGAAGCCACA190.1735952489721334No Hit
AAGCGGGGGGAACATGCTGGCTGAGGACTACACAAACCAGGTTTCCCACCAT190.1735952489721334No Hit
AAGCGGGGGGGGGCTTCCTGCCCAGAGCTTCAACCCTGCCAAGGCGGAGGGA180.16445865692096848No Hit
AAGCGGGGGACTGAAGTGATGGCGGCGGCCGAGCGGGCATGGGGAGCAGCGG170.15532206486980357No Hit
AAGCGGGGGGATTCAGAGGGTACAGAACAACCTCTATTATTTCAGAGTACCC170.15532206486980357No Hit
AAGCGGGGGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAACAAAGCACC170.15532206486980357No Hit
AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG170.15532206486980357No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT160.14618547281863864No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC160.14618547281863864No Hit
AAGCGGGGGATCAGATTAGCAGCTAAACAGTTGTATTAGATAATCTTCAAAT160.14618547281863864No Hit
AAGCGGGGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAAC150.13704888076747374No Hit
AAGCGGGGGGAACAGACACCATGATCATCGCAACTCTTGGTCATGTTCTTGA150.13704888076747374No Hit
AAGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC140.1279122887163088No Hit
AAGCGGGGGAAAGACAAATGGTCAGTCATCTGAAAGTGTTGCTGCGTGCTAA140.1279122887163088No Hit
AAGCGGGGGGCAGTTTGAATCGCGGTCCGACGGAGGAGTGGGCGCTGGGATC140.1279122887163088No Hit
AAGCGGGGGATCTGTTTGGGGCATCTTTGGGAGGCTTTTTGGCTCAAAAATT130.11877569666514391No Hit
AAGCGGGGGGGTGCTGTGCGCGCGCATACCTACCCTATGCGCGCGCATACCT130.11877569666514391No Hit
AAGCGGGGGAACACTTAGCTTTTAACAGCAGGCTTCTCTGCAAGGCTTGGTC130.11877569666514391No Hit
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA130.11877569666514391No Hit
AAGCGGGGGATTCCTATATTTGGAAAGAGTACAGTTTCTAACGGCAGCAGAA110.10050251256281408No Hit
AAGCGGGGGATTTCAAATAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.10050251256281408No Hit
AAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.10050251256281408No Hit
AAGCGGGGGGAGAAAAGGGGAAGTCAGCCCTGAGAAGGGGAGACAAGCTGAA110.10050251256281408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTT206.03902E-446.032
AGGGGTT206.03902E-446.031
TGAGAAC301.6964477E-646.010
CAGCTAA452.5465852E-1046.020
GCGGGGG8500.046.03
GCGGGGC206.03902E-446.03
GCGGGGA900.046.03
TTTGAGG206.03902E-446.017
TTTGAGA253.198185E-546.022
CTCTGCC404.783942E-946.042
TGTGAGC206.03902E-446.030
ACAGTCA301.6964477E-646.036
GAGGCGC206.03902E-446.09
CGACAAG253.198185E-546.019
GAGAATT206.03902E-446.09
CGCTGAG206.03902E-446.026
TGTGACG301.6964477E-646.029
GAGCGTC253.198185E-546.038
TTTTGCC206.03902E-446.020
TTGTTGT206.03902E-446.031
Produced by FastQC (version 0.11.5)