FastQCFastQC Report
Fri 17 Jun 2016
SRR1527334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13584
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGCTGTCCTGGAACTCTCAAGAGATTTTCCTGCCTCTGCCTCTG4823.54829210836278No Hit
AAGCGGGGGGGGAAAGAGAACGTGAACGAGAAAAGGAAAAAGAACGGGAGCG3772.775323910482921No Hit
AAGCGGGGGAGTCTTGGGAACCATGCAGTTCCAGAGGGACGTCCCCTCCCTG3632.67226148409894No Hit
AAGCGGGGGGCTCCTAACCGACAAGCGTCTGTGAGGGGAGCGTCCAGGTCCC2932.1569493521790344No Hit
AAGCGGGGATGAGAACAACCCACGTGGGCATGGCCACAGTCATGGCAAAGGC2581.8992932862190812No Hit
AAGCGGGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG2451.8035924617196701No Hit
AAGCGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG2231.6416372202591283No Hit
AAGCGGGGCACAAGAACTAGCCAGGGTCCCTTTGCTTTCCTGTGTTCAAGCC2041.5017667844522968No Hit
AAGCGGGGGAGCTCGGAGTGTAACCCTCTGTGACGGTCGCTGTCTCAGGAAT1851.3618963486454652No Hit
AAGCGGGGGACCCTGGAAACCACAGAGAGCGGCTGCTGAGCCCAAGAGGACC1801.325088339222615No Hit
AAGCGGGGGGGGAACCAGTGACTGGGCACGCATGTGGGGGTTACTCACAGGA1781.3103651354534747No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC1731.2735571260306242No Hit
AAGCGGGGGAGCCCTTTGCCGCGAATAGCCGGAAGAAAATACATCTTTTGCA1571.155771495877503No Hit
AAGCGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT1471.0821554770318023No Hit
AAGCGGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA1401.0306242638398115No Hit
AAGCGGGGAACACACACACACATACATACACCCAACAACAACAGCAACAATA1371.0085394581861014No Hit
AAGCGGGGGATCAGATTGGCAGCTAAACAGTTGTATTAGATAATCTTCAAAT1330.979093050647821No Hit
AAGCGGGGGACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCC1290.9496466431095406No Hit
AAGCGGGGGAGTCTGAAACCATCTGAGCAGAGTCTCTCTTCAGTCCCCGGGA1290.9496466431095406No Hit
AAGCGGGGGATGTGGGAACTGAACCAGGGTCCTTTTAGGAAAGCAACAAGGG1260.9275618374558304No Hit
AAGCGGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA1200.88339222614841No Hit
AAGCGGCTCACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAA1200.88339222614841No Hit
AAGCGGGGGAACTACTGTATGGAGAGGAGCGGTGTTGGGAAATGTGAACCCT1130.8318610129564193No Hit
AAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGC1050.7729681978798587No Hit
AAGCGGGGGAGAGCCACCAGCCATGCCCCAGCCAGTGCCTACAGCATAACAG1050.7729681978798587No Hit
AAGCGGGGGATAATAGATTCTGCCAGCTGTTAGCTGTTCAGTTCATAAGCTT1000.7361601884570083No Hit
AAGCGGGGGAGAATTCTCAAGGTCTGTTTTTTGTTGTTTTGTTTTTGTTTTT1000.7361601884570083No Hit
AAGCGGGGGAAGCGAGGCACGAGTCCACGGCCTCCTGAGGGCGGCCTGGGCT990.7287985865724381No Hit
AAGCGGGGGGAAGGTGTATGAGTTTCGACCCCAGAGCTCTGTGTGCTTTCAT950.6993521790341578No Hit
AAGCGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATT950.6993521790341578No Hit
AAGCGGTCTGGAAACGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTA900.6625441696113075No Hit
AAGCGGGGGAACAGCAAGCTGGCGCCTGCGCTCTCTGTCAAGGCGGCCCAGA900.6625441696113075No Hit
AAGCGGGGGGTCTGGAATTTGATGAAGTTAAAGTCAAGCAAATCCTGAAGAA890.6551825677267373No Hit
AAGCGGGGGGGGAGCTGACATCACACATGGGTGGCATATGTGGGATGTAGGG890.6551825677267373No Hit
AAGCGGAGCAGTCCAAATGTTTTTGAGATGGCAGCAACGGAAACCATAACGA890.6551825677267373No Hit
AAGCGGGGACGCACTGACAGGCCTCAAGACCCCAAGCTCTGAGAGAAGGGCT860.633097762073027No Hit
AAGCGGGGGACTTCAACAAAAAGAGAGAAAGAACTATGCTAAGGACATATAT830.6110129564193169No Hit
AAGCGGGGGTTCCTGTTTGCAGGCTTAACTCCGGCCGGGGTTCCTCGCTCGA790.5815665488810364No Hit
AAGCGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAG770.5668433451118964No Hit
AAGCGGGGGGCTGATGGCAAAGTTGATCTTTGCCTGTCATGAGAAGCTGCAT760.5594817432273262No Hit
AAGCGGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT730.5373969375736161No Hit
AAGCGGGGGACTCCAGACATGCTCTGAGTGTCACGTCCGCCCTGGATCTACT730.5373969375736161No Hit
AAGCGGGGGGAGCAGTGTGAGGAAGCAGCGAGGACGACCCCCGGACGGAGCA700.5153121319199058No Hit
AAGCGGGGGGCTGGCCGAGCAGGAGGCGCCATCATGGGTGTTGACATTCGCC680.5005889281507656No Hit
AAGCGGGGGATCGACACCTTCCATCAATACTCTAGGAAGGAAGGACACCCTG670.4932273262661956No Hit
AAGCGGGGGGCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGCAGTGTG670.4932273262661956No Hit
AAGCGGGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGC660.48586572438162545No Hit
AAGCGGGGGGCGCAAGGTGCGCGGGTCCGGTTATGAAGCTTGGCCGGGCCGC650.4785041224970554No Hit
AAGCGGGGGAGAGGAAGCGAAGGATAGCTTTGTATTAATGTTGCGTTAAAGC650.4785041224970554No Hit
AAGCGGGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCG640.47114252061248524No Hit
AAGCGGGGGGTTTGAAAGGAAATCTTTCGTGACAATGCCGTCTGAACTGGAG630.4637809187279152No Hit
AAGCGGGGGAAGATAGCCACCCGGAAGGCCTATGGACTGGCCCTCGCTAAGC620.45641931684334514No Hit
AAGCGGGGGAGGCGCCTTTGAGGACTCCCTGTGAGCCAGGCAGTATCGCAGT610.449057714958775No Hit
AAGCGGGGGACCGGAACTTGGTAGCTCTGAGAGACATGGGACTGACGCTCCG600.441696113074205No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC590.43433451118963484No Hit
AAGCGGGGGGGCCATGGGTGGAAGTGTGTAGGTTTTATGCAAATATTTTGCT560.4122497055359246No Hit
AAGCGGGGGAGACAAGCACACGTTGACCACGTGTGGAAAAATAAAGTTGCTG540.3975265017667844No Hit
AAGCGGGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTAGGTC540.3975265017667844No Hit
AAGCGGGGGGCTGGGGTACAGTGGATGTGAATACTGTAAATAGCTTGTGCTA540.3975265017667844No Hit
AAGCGGGGGGATCTCTCTCGCAGCACTGCCACAGGTGCTAGTAGGGCCAATT530.39016489988221437No Hit
AAGCGGGTATCGGAGGCAGCTATATATAAGAGGGGAGGTATCCCACTAGGTG530.39016489988221437No Hit
AAGCGGGGGCCCCTTTCTGCGCAGGAAGGCTGAGGTGCTAGCACCAGCCTGG530.39016489988221437No Hit
AAGCGGGGGAGAAAACCATCCTGAGACTCCTAAAAAAAAGTCTGATCCTGAG520.3828032979976443No Hit
AAGCGGGGGACCCTGAGCAAGTAGGAATTCAGACAAATGGTGGAAGCACAGT510.3754416961130742No Hit
AAGCGGGGGGAGTGTGGTGCAGTGGGTGCAGTGCTATGCAGTGCACGCAGTG490.36071849234393405No Hit
AAGCGGGGGAGATGGAGCGCAGCATAACCACCATCATCGACACCTTCCATCA490.36071849234393405No Hit
AAGCGGGGGGAGTGTGACACCCTTGTAAGAGAGCTAGAGCAAGATTCGGAGA490.36071849234393405No Hit
AAGCGGGGGGTGGTTTTTTTTTTCCAGTTTTGGGGGTTTTTTGTTTTGTTTT480.35335689045936397No Hit
AAGCGGGGAGATAGGCACAGCATCAAGGCAAGACACAGTAGTGCTCCCTGCT480.35335689045936397No Hit
AAGCGGGGGGGTCTTGGCGGGCTGGCAGCAGGCAGGCTGCAGCAGGCGAGCA470.34599528857479384No Hit
AAGCGGGGAACTCAGAGGGTTGATACTTTTAATTTGTAATTTTTTGTAAAAC460.3386336866902238No Hit
AAGCGGGGGAGCCTATGGAGGTGTTTGTGGACGACGAGACCAAGCTCACACT460.3386336866902238No Hit
AAGCGGGGGTCTTCCGGTCGGGCCGAGCTCGTCGCTAGGTTTGTTGAAGGAC460.3386336866902238No Hit
AAGCGGCATGGTCAATATAACCAGTAGTGTTAACAGTCGGGAGAGGAGTGGC450.33127208480565373No Hit
AAGCGGGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCTCC450.33127208480565373No Hit
AAGCGGGGGATTATATCACATTTCCAAAAAGTAGTACCATGAAGATGGCGAG430.3165488810365135No Hit
AAGCGGGGGATCAGGCAAGACCCATGAGGCAGAGATCGTAGAAGGAGAGAAC430.3165488810365135No Hit
AAGCGGGGGGCCGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA420.30918727915194344No Hit
AAGCGGGGGACCCTTTGTGGGGGGAAAATGAGCCTTTGGACATAGACAGTAA420.30918727915194344No Hit
AAGCGGGGGGAGATGGGTAGTGGAGGTAAAGAGGAGGTCATAGTATGCAGGG420.30918727915194344No Hit
AAGCGGGGGATGACAACTCACTCAAGATTGTCAGCAATGCATCCTGCACCAC420.30918727915194344No Hit
AAGCGGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTTCA420.30918727915194344No Hit
AAGCGGGGGGAGTCTTGGGAACCATGCAGTTCCAGAGGGACGTCCCCTCCCT420.30918727915194344No Hit
AAGCGGGGCATACACTGTGTCCCCGGTGTAATGTTTGTAACTGATGTCTGTT420.30918727915194344No Hit
AAGCGGGGGGTCATTACCTGATTCTGTCGTGAGTGGGTACTTTGGCTCATTT400.2944640753828033No Hit
AAGCGGGGGATGAGTGTGATTTTGATCTGTTTTCCAAGTGCACCCAAAGTCT390.2871024734982332No Hit
AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCT380.2797408716136631No Hit
AAGCGGGGGGAACAGACACCATGATCATCGCAACTCTTGGTCATGTTCTTGA380.2797408716136631No Hit
AAGCGGGGGGAAGTTGAAGAAAACAGAAACGCAAGAGAAAAATCCTCTGCCT370.27237926972909304No Hit
AAGCGGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA370.27237926972909304No Hit
AAGCGGGGAACCACTTGTAGCTTATTTCCATCATAATTTGATGATAGATTGA360.26501766784452296No Hit
AAGCGGGGGGAAGGTTGAATATTGAGTGTCCCGGGAGGTCAGATTGCTGTCA360.26501766784452296No Hit
AAGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC350.2576560659599529No Hit
AAGCGGGGGAGACGGCGGGACAGACGGCTGGGTGTGCAGCGACCTCGAACCC350.2576560659599529No Hit
AAGCGGGGGGCTGGCAAAGAAAAGCAAAGAAGTATTCAGAAAAGAGATGTCT350.2576560659599529No Hit
AAGCGGGGGAAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC340.2502944640753828No Hit
AAGCGGGGGGAGCTGACCGGCGGCCCTTACATCCCCCTGGGTGGTGACTTCG340.2502944640753828No Hit
AAGCGGGGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAAC340.2502944640753828No Hit
AAGCGGGGGACCTTATGGATCTGAGCCACCTCATCCTGGACAAGGCCTCTGG340.2502944640753828No Hit
AAGCGGGGGAAACCAGCCTCAGTTTCAGAGGAAGAGCTGCTGAATTCAATCA340.2502944640753828No Hit
AAGCGGGGGGGCTATCTGCTGCCTGGCGCTGGCTGACGGATTGCTGCAGCAC340.2502944640753828No Hit
AAGCGGGGGGGACATTGGCGTCTTTGCTAAGGGTCACATTGAAGTAACGCTA340.2502944640753828No Hit
AAGCGGGGGGTCAGCGACCCAGCACAAAAGCTTTGTCTCCAGACCCTCTCTT340.2502944640753828No Hit
AAGCGGCAAAGCCTGAAGGTCTCCACTGAGGACTGTGAGCGAGAGCAGCTGA330.24293286219081273No Hit
AAGCGGGGGTAGCACTGGGCATTTCGCCTAACAATACTGTGACTCAGTATTC320.23557126030624262No Hit
AAGCGGGGGACCTTAACTTGGTGCTACCCTGTCTGGAGTACCACAACAACAC320.23557126030624262No Hit
AAGCGGGGGATACAATGGCGCTATTAATGTCAGCTTTTCAAAATACAGGCTT310.22820965842167257No Hit
AAGCGGGGGATGCACTGTACATTCCACGAGCATTGCCTTCTTATTTTACTTC310.22820965842167257No Hit
AAGCGGGGGGGAGCAGTGTGAGGAAGCAGCGAGGACGACCCCCGGACGGAGC300.2208480565371025No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT300.2208480565371025No Hit
AAGCGGGGGATTAGCCAGATTGTGTCTTCCATCACTGCTTCCCTCAGATTTG300.2208480565371025No Hit
AAGCGGGGGATGTTTGGAAAGCCTTTGAAAGGGCAGTAAGCATGTGGTGATA290.21348645465253238No Hit
AAGCGGGGGCAGCATGGAAGGTCCAGCGTTCTCAAAATCCCTTAAAGATAAG280.2061248527679623No Hit
AAGCGGGGGTTTGGTGGAGTGATGATGTGGTCTCAGTAGGAACGGTAACATT280.2061248527679623No Hit
AAGCGGGGGATAACGGAAAACGACCACGTTCTCCTCTTTTGGAAAAGCCTAG280.2061248527679623No Hit
AAGCGGCATGGTCAATATAACCAGCAGTGTTAACAGTCGGGAGAGGAGTGGC270.1987632508833922No Hit
AAGCGGGGGATCAGATTAGCAGCTAAACAGTTGTATTAGATAATCTTCAAAT270.1987632508833922No Hit
AAGCGGGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC260.19140164899882214No Hit
AAGCGGGGGGAAGGTATAGGGAACTTTCGGGATAGCATGTGAAATGTGAATA260.19140164899882214No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT260.19140164899882214No Hit
AAGCGGGGGGCAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAA260.19140164899882214No Hit
AAGCGGGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACCCGACTGTT260.19140164899882214No Hit
AAGCGGGGGAGCACCCCGTGGGGTCCCTCAGATTGAGGTTACTTTTGACATC230.1693168433451119No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT230.1693168433451119No Hit
AAGCGGGGGATCCCAGGCTCAGTGCCTTCCTGCCACCTGCTGCAGGTATAGG220.16195524146054183No Hit
AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG220.16195524146054183No Hit
AAGCGGGGGGAACATGCTGGCTGAGGACTACACAAACCAGGTTTCCCACCAT220.16195524146054183No Hit
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA220.16195524146054183No Hit
AAGCGGGGGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAACAAAGCACC210.15459363957597172No Hit
AAGCGGGGGACTGAAGTGATGGCGGCGGCCGAGCGGGCATGGGGAGCAGCGG200.14723203769140164No Hit
AAGCGGGGGATCTGTTTGGGGCATCTTTGGGAGGCTTTTTGGCTCAAAAATT200.14723203769140164No Hit
AAGCGGGGGGGGTGATCCATTCATGCTGCAGCAGTCTACAAACCCAGCACCA200.14723203769140164No Hit
AAGCGGGGGAGGCCTTTGCCACCAACCCCTCTGCTTGCTTGAGGAAGCCACA200.14723203769140164No Hit
AAGCGGGGGGATTCAGAGGGTACAGAACAACCTCTATTATTTCAGAGTACCC200.14723203769140164No Hit
AAGCGGGGGGCTCGGTTGGTACGTGCTTAGTGCTGGTATGGCTTCCAGCGGA180.13250883392226148No Hit
AAGCGGGGGGGGGCTTCCTGCCCAGAGCTTCAACCCTGCCAAGGCGGAGGGA180.13250883392226148No Hit
AAGCGGGGGGAGAAAAGGGGAAGTCAGCCCTGAGAAGGGGAGACAAGCTGAA180.13250883392226148No Hit
AAGCGGGGGCATTTTTGCTGGTTTAAAAAAAGATTTCTTTATGCCTATATGT180.13250883392226148No Hit
AAGCGGGGGATGTGCAGCTGCCAGCAGATGAGGTGGACACACAACTGCTACA170.1251472320376914No Hit
AAGCGGGGGATTCCTATATTTGGAAAGAGTACAGTTTCTAACGGCAGCAGAA160.11778563015312131No Hit
AAGCGGGGGAAAGACAAATGGTCAGTCATCTGAAAGTGTTGCTGCGTGCTAA160.11778563015312131No Hit
AAGCGGGGGAACACTTAGCTTTTAACAGCAGGCTTCTCTGCAAGGCTTGGTC150.11042402826855124No Hit
AAGCGGGGGGATATGATTAGTATTGTAGGAACATTTGAGTTATTTCAATCAG150.11042402826855124No Hit
AAGCGGGGGGACTTTCCATTGCTCTGCATGTAGGTTTTGACCACTTCGAGCA140.10306242638398115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTT359.23319E-846.00000431
CGCTGAG359.23319E-846.00000426
TTTTGCC359.23319E-846.00000420
GAGAACG359.23319E-846.00000416
GTTGACC359.23319E-846.00000435
CCAACCA359.23319E-846.00000434
GGTTGAC359.23319E-846.00000434
GTGTTCA359.23319E-846.00000442
TCAAGCC359.23319E-846.00000446
TCAAGAG359.23319E-846.00000425
AAATTTT359.23319E-846.00000410
AATTTTA359.23319E-846.00000411
TGTCCTG359.23319E-846.00000412
AACGAGA359.23319E-846.00000425
TGACCAA359.23319E-846.00000437
TTCAAGC359.23319E-846.00000445
TGCCGCT359.23319E-846.00000423
CAACCAG359.23319E-846.00000435
CAACCAA359.23319E-846.00000431
GAAGCGC359.23319E-846.00000446
Produced by FastQC (version 0.11.5)