FastQCFastQC Report
Fri 17 Jun 2016
SRR1527333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195806
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC161911.3539821676760277No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG77010.6440007827356611No Hit
TTTAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT44910.37556259125644126No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT44260.3701269269429991No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT26260.21960083826306273No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG21370.17870791750501336No Hit
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC19010.15897227476697726No Hit
TTTAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT15460.12928518505510092No Hit
TTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT14050.11749397477517257No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC12440.10403025239880048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT302.5278023E-644.00000430
GATAACG207.856609E-444.00000414
CCGATAA207.856609E-444.00000444
TTACTCG207.856609E-444.00000444
GCGTTAA207.856609E-444.00000427
CCGTATC207.856609E-444.00000426
CCCGTAT600.044.00000444
CCCCGTA207.856609E-444.00000439
TACGCAC408.312782E-944.00000440
CATACCG207.856609E-444.00000431
CGTACGC408.312782E-944.00000424
ATGCGTA207.856609E-444.00000438
TACTACG207.856609E-444.00000431
CGGTATA207.856609E-444.00000416
ATTGCGC302.5278023E-644.00000425
GCGCTTA207.856609E-444.00000416
CGATACT207.856609E-444.00000426
AATACCG207.856609E-444.00000440
TCGGTAT207.856609E-444.00000415
GTAACGT302.5278023E-644.00000433
Produced by FastQC (version 0.11.5)