##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527333_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1195806
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.399161736937263	31.0	31.0	33.0	30.0	34.0
2	31.324123645474266	33.0	31.0	34.0	30.0	34.0
3	31.479172206862987	31.0	31.0	34.0	30.0	34.0
4	35.0792937984924	35.0	35.0	37.0	33.0	37.0
5	35.61573616456181	37.0	35.0	37.0	35.0	37.0
6	36.07760623378709	37.0	35.0	37.0	35.0	37.0
7	36.53311406699749	37.0	37.0	37.0	35.0	37.0
8	36.60708676825505	37.0	37.0	37.0	35.0	37.0
9	38.36276118367026	39.0	39.0	39.0	37.0	39.0
10	34.6833240508912	35.0	35.0	37.0	31.0	39.0
11	34.43993507308041	35.0	33.0	39.0	26.0	39.0
12	34.74683184396131	35.0	33.0	39.0	27.0	39.0
13	35.13057051060122	35.0	34.0	39.0	31.0	39.0
14	35.82818366858838	35.0	35.0	40.0	32.0	40.0
15	35.90971528826582	35.0	35.0	40.0	32.0	40.0
16	36.0997034636053	36.0	35.0	40.0	33.0	40.0
17	35.98773965007702	35.0	35.0	40.0	32.0	40.0
18	35.87710381115331	35.0	34.0	40.0	32.0	40.0
19	35.79921743159007	35.0	34.0	40.0	32.0	40.0
20	35.63135659128655	35.0	34.0	40.0	31.0	40.0
21	35.53432747452346	35.0	34.0	40.0	31.0	40.0
22	35.39610103980077	35.0	34.0	39.0	31.0	40.0
23	35.24654584439282	35.0	34.0	39.0	31.0	40.0
24	35.16783909764627	35.0	34.0	38.0	31.0	40.0
25	34.934306233619836	35.0	34.0	38.0	30.0	40.0
26	34.53996467654452	35.0	34.0	38.0	30.0	40.0
27	34.426470514448	35.0	33.0	38.0	30.0	40.0
28	34.28882276891068	35.0	33.0	38.0	30.0	40.0
29	34.05057174826017	35.0	33.0	38.0	29.0	39.0
30	33.73380631975421	34.0	33.0	38.0	29.0	39.0
31	33.5831489388747	34.0	32.0	37.0	28.0	39.0
32	33.50295951015466	34.0	32.0	37.0	27.0	39.0
33	32.98360520017461	34.0	32.0	37.0	25.0	38.0
34	32.418291930296384	34.0	31.0	36.0	23.0	38.0
35	32.31073685865433	34.0	31.0	36.0	23.0	38.0
36	32.236313415386775	34.0	31.0	36.0	23.0	38.0
37	32.23588525229009	34.0	31.0	36.0	24.0	38.0
38	31.797466311425097	33.0	30.0	35.0	23.0	38.0
39	31.042146468574334	33.0	29.0	35.0	23.0	38.0
40	30.796299734237827	33.0	29.0	35.0	22.0	37.0
41	30.593143034907	32.0	29.0	34.0	22.0	37.0
42	30.227199060717208	32.0	28.0	34.0	22.0	37.0
43	29.65947068337172	31.0	27.0	34.0	21.0	36.0
44	29.207692552136383	31.0	27.0	34.0	20.0	36.0
45	28.790067954166478	31.0	25.0	34.0	20.0	36.0
46	28.68287749016145	31.0	24.0	34.0	20.0	35.0
47	28.24271244666777	30.0	24.0	33.0	19.0	35.0
48	27.78888297934615	30.0	23.0	33.0	18.0	35.0
49	27.312418569567303	29.0	24.0	32.0	18.0	34.0
50	30.771116719601675	32.0	29.0	34.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	9.0
12	27.0
13	51.0
14	125.0
15	237.0
16	438.0
17	659.0
18	1065.0
19	1601.0
20	2441.0
21	3424.0
22	4727.0
23	6318.0
24	8661.0
25	11338.0
26	14546.0
27	19401.0
28	26386.0
29	35979.0
30	55253.0
31	99061.0
32	180779.0
33	191460.0
34	113506.0
35	188299.0
36	198315.0
37	31103.0
38	591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.30615835679032	0.5793581902081106	0.09056653002242838	0.023916922979145445
8	99.23106256365999	0.569657620048737	0.12058812215359348	0.07869169413767785
9	94.53615385773277	2.909000289344593	1.9137719663557466	0.6410738865668846
10	49.60336375632836	40.27827256260631	3.761897832926077	6.356465848139246
11	40.01067062717531	20.223681767778384	25.356872268578684	14.408775336467622
12	34.093573706771835	17.19794013410202	23.502558107251513	25.205928051874636
13	26.733182472742236	17.005684868615813	28.23551646337282	28.02561619526913
14	26.981968647088237	18.00057868918537	29.76151650016809	25.255936163558303
15	29.206493360963233	21.350704043967	26.941326603144656	22.501475991925112
16	35.11673298177129	23.79666935941114	22.434993636091473	18.651604022726094
17	30.990729265449414	23.465846466734572	24.367246861112925	21.176177406703093
18	31.376326929284517	23.297006370598574	23.602156202594735	21.72451049752217
19	29.243288627084997	26.847665925743808	22.714470407407223	21.194575039763976
20	31.061894655153093	24.99619503498059	23.064443563588075	20.87746674627824
21	28.93646628299239	26.335960849836848	25.26078644863799	19.466786418532774
22	27.86890181183235	20.638464767696433	29.151467712990236	22.34116570748098
23	28.11885874464587	22.358727084493637	27.732508450367366	21.789905720493124
24	30.484627105065538	22.43298662157574	24.75217552010945	22.330210753249272
25	26.826926775747907	23.817324883802222	23.92704167732893	25.42870666312094
26	24.58743307860974	25.862639926543267	26.171636536361248	23.37829045848574
27	23.24022458492431	22.932900487202772	25.728588082013303	28.09828684585961
28	22.933820368855816	27.188022137370112	24.197236006509417	25.68092148726466
29	29.04492869244677	24.618123675579483	22.926043187607352	23.41090444436639
30	26.985062794466664	22.022970281132558	26.252502496224306	24.73946442817648
31	28.87215819288413	22.17174023211123	25.190624566192177	23.765477008812468
32	30.94122290739468	22.90037012692694	24.427791799004186	21.730615166674195
33	31.262010727492584	22.33731892965916	25.18878480288609	21.211885539962168
34	26.690031660653986	24.049720439603078	28.870067552763572	20.39018034697936
35	24.056410487988856	28.894068101347543	26.451698686910753	20.59782272375285
36	24.703505418102935	30.057718392448272	24.010416405336652	21.22835978411214
37	29.466987119984346	27.388389086524068	21.88264651624093	21.261977277250658
38	27.096033972065705	29.524270659287545	21.835899803145324	21.543795565501426
39	29.37959836294516	24.56025475704253	23.257451459517682	22.80269542049463
40	26.53381903084614	23.648401162061404	22.568376475782863	27.249403331309598
41	23.49979846229238	27.57955721914759	23.20543633331828	25.715207985241754
42	22.768659799332	24.350019986519552	23.197910028884284	29.683410185264165
43	22.41467261411968	23.519199602611128	25.91281528943658	28.153312493832612
44	24.674236456415173	22.571637874370925	23.910065679550026	28.844059989663872
45	26.19070317426071	23.773588692480217	23.838482161822235	26.19722597143684
46	24.861892313636158	26.933549421896192	22.499218100594913	25.705340163872737
47	23.69723851527756	24.814894723726088	25.29365131133311	26.19421544966324
48	25.869497226138687	23.133016559542266	27.23744486982002	23.760041344499026
49	27.58014259838134	22.662957034836754	25.234611634328648	24.522288732453255
50	24.162949508532318	22.487008762290873	25.64939463424669	27.700647094930115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	15.5
6	31.0
7	56.0
8	81.0
9	310.0
10	539.0
11	1044.5
12	1550.0
13	1962.5
14	2375.0
15	2152.0
16	1929.0
17	1943.0
18	1957.0
19	1974.5
20	1992.0
21	2563.0
22	3134.0
23	4778.5
24	6423.0
25	6700.5
26	6978.0
27	7953.5
28	8929.0
29	10312.0
30	11695.0
31	15886.5
32	20078.0
33	21428.0
34	22778.0
35	25617.5
36	28457.0
37	29492.5
38	30528.0
39	37964.5
40	45401.0
41	44988.5
42	44576.0
43	46152.0
44	47728.0
45	52011.5
46	56295.0
47	61410.0
48	66525.0
49	68705.0
50	70885.0
51	75558.0
52	80231.0
53	82101.0
54	83971.0
55	89553.5
56	95136.0
57	98738.0
58	102340.0
59	104270.0
60	106200.0
61	104599.5
62	102999.0
63	80903.0
64	58807.0
65	46934.5
66	35062.0
67	27582.5
68	20103.0
69	16298.5
70	12494.0
71	9786.0
72	7078.0
73	6610.5
74	6143.0
75	4273.5
76	2404.0
77	1713.0
78	1022.0
79	884.0
80	746.0
81	431.5
82	117.0
83	101.0
84	85.0
85	44.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1195806.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.100209601299495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55464363038868	7.9427154224360415
2	9.300586580946103	2.064769209270703
3	3.2464999482939167	1.0811048969001136
4	1.4437853425048008	0.6410527968434908
5	0.796573289791441	0.442106523974084
6	0.48556654246918585	0.32339342380717534
7	0.3943529417964263	0.30641802175805744
8	0.3299304922921066	0.29298380946418484
9	0.2905567959347826	0.29027172023622827
>10	7.789490679203867	23.819895728940384
>50	2.6519813296565613	20.249841931732963
>100	1.6607985787720831	35.23292427350532
>500	0.043884427138396735	3.2060471994062456
>1k	0.00983616470343375	2.0998504723117115
>5k	7.566280541102885E-4	0.646786196595172
>10k+	7.566280541102885E-4	1.3598383728181314
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	16191	1.3539821676760277	No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7701	0.6440007827356611	No Hit
TTTAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	4491	0.37556259125644126	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	4426	0.3701269269429991	No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	2626	0.21960083826306273	No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2137	0.17870791750501336	No Hit
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1901	0.15897227476697726	No Hit
TTTAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1546	0.12928518505510092	No Hit
TTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1405	0.11749397477517257	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1244	0.10403025239880048	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	9.198816530440557E-4	0.0	0.0	0.0	0.0
8	0.002090640120554672	0.0	0.0	0.0	0.0
9	0.004766659474864652	0.0	0.0	0.0	0.0
10	0.02082277560072453	0.0	0.0	0.0	0.0
11	0.03161047862278664	0.0	0.0	0.0	0.0
12	0.0413946743869825	0.0	0.0	0.0	0.0
13	0.049924486078845566	0.0	0.0	0.0	0.0
14	0.058705174585175185	0.0	0.0	0.0	0.0
15	0.06748586309150481	0.0	0.0	0.0	0.0
16	0.0764338028074788	0.0	0.0	0.0	0.0
17	0.08479636328969749	0.0	0.0	0.0	0.0
18	0.0949986870780043	0.0	0.0	0.0	0.0
19	0.1064553949386439	0.0	0.0	0.0	0.0
20	0.11707584675106163	0.0	0.0	0.0	0.0
21	0.12485302799952501	0.0	0.0	0.0	0.0
22	0.13263020924798838	0.0	0.0	0.0	0.0
23	0.1408255185205627	0.0	0.0	0.0	0.0
24	0.14952258142207014	0.0	0.0	0.0	0.0
25	0.157968767509111	0.0	0.0	0.0	0.0
26	0.16775296327330688	0.0	0.0	0.0	0.0
27	0.1751956421024815	0.0	0.0	0.0	0.0
28	0.18389270500398894	0.0	0.0	0.0	0.0
29	0.19275701911514076	0.0	0.0	0.0	0.0
30	0.20170495883111475	0.0	0.0	0.0	0.0
31	0.21015114491815562	0.0	0.0	0.0	0.0
32	0.21884820781966305	0.0	0.0	0.0	0.0
33	0.22645813785848207	0.0	0.0	0.0	0.0
34	0.2339844422924789	0.0	0.0	0.0	0.0
35	0.244772145314541	0.0	0.0	0.0	0.0
36	0.2639224088188218	0.0	0.0	0.0	0.0
37	0.2768843775662608	0.0	0.0	0.0	0.0
38	0.28775570619314506	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGT	30	2.5278023E-6	44.000004	30
GATAACG	20	7.856609E-4	44.000004	14
CCGATAA	20	7.856609E-4	44.000004	44
TTACTCG	20	7.856609E-4	44.000004	44
GCGTTAA	20	7.856609E-4	44.000004	27
CCGTATC	20	7.856609E-4	44.000004	26
CCCGTAT	60	0.0	44.000004	44
CCCCGTA	20	7.856609E-4	44.000004	39
TACGCAC	40	8.312782E-9	44.000004	40
CATACCG	20	7.856609E-4	44.000004	31
CGTACGC	40	8.312782E-9	44.000004	24
ATGCGTA	20	7.856609E-4	44.000004	38
TACTACG	20	7.856609E-4	44.000004	31
CGGTATA	20	7.856609E-4	44.000004	16
ATTGCGC	30	2.5278023E-6	44.000004	25
GCGCTTA	20	7.856609E-4	44.000004	16
CGATACT	20	7.856609E-4	44.000004	26
AATACCG	20	7.856609E-4	44.000004	40
TCGGTAT	20	7.856609E-4	44.000004	15
GTAACGT	30	2.5278023E-6	44.000004	33
>>END_MODULE