FastQCFastQC Report
Fri 17 Jun 2016
SRR1527332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027970
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC132841.292255610572293No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT50010.4864927964823876No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC35400.3443680263042696No Hit
TTTAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC25610.24913178400147862No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT21930.21333307392239076No Hit
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA19260.18735955329435686No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT18090.1759778981876903No Hit
TTTAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG13410.13045127776102414No Hit
TTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT12640.12296078679338893No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC12230.11897234355088185No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT11510.11196824810062551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA551.8189894E-1246.00000428
AATCCGT351.0194344E-746.00000430
CGCGACA351.0194344E-746.00000425
CGATGCG351.0194344E-746.00000421
TCACGCG206.3111435E-446.039
CTCGTCA206.3111435E-446.039
CGGCATA301.86121E-646.037
CGGTTAG405.6097633E-946.029
CGGTTAC253.4169017E-546.029
CGCATAG405.6097633E-946.023
TTCACGC206.3111435E-446.042
CACGCGC206.3111435E-446.040
ATTCGTC206.3111435E-446.014
ATTCGAA650.046.010
GCGTTAG206.3111435E-446.016
ACGATAT301.86121E-646.033
TACGCCT253.4169017E-546.026
CGTAGTG206.3111435E-446.026
TATTCGA301.86121E-646.018
CATACCG253.4169017E-546.031
Produced by FastQC (version 0.11.5)