##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1027970
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.892718659104837	33.0	31.0	33.0	31.0	34.0
2	32.34681362296565	33.0	31.0	34.0	31.0	34.0
3	32.341235639172346	33.0	31.0	34.0	31.0	34.0
4	35.67822115431384	37.0	35.0	37.0	35.0	37.0
5	36.081450820549236	37.0	35.0	37.0	35.0	37.0
6	36.3569073027423	37.0	37.0	37.0	35.0	37.0
7	36.61675437999163	37.0	37.0	37.0	35.0	37.0
8	36.653678609297934	37.0	37.0	37.0	35.0	37.0
9	38.43986400381334	39.0	39.0	39.0	38.0	39.0
10	35.11275912721188	35.0	35.0	37.0	33.0	39.0
11	34.84402073990486	35.0	34.0	39.0	27.0	39.0
12	35.20325690438437	35.0	34.0	39.0	31.0	39.0
13	35.55115908051791	35.0	35.0	39.0	32.0	39.0
14	36.205468058406375	36.0	35.0	40.0	33.0	40.0
15	36.31396441530395	36.0	35.0	40.0	33.0	40.0
16	36.50251661040692	36.0	35.0	40.0	33.0	40.0
17	36.279135577886514	36.0	35.0	40.0	32.0	40.0
18	36.195699290835336	36.0	35.0	40.0	32.0	40.0
19	35.9893956049301	36.0	35.0	40.0	31.0	40.0
20	35.90839713221203	36.0	35.0	40.0	31.0	40.0
21	35.89320991857739	36.0	35.0	40.0	31.0	40.0
22	36.01230969775383	35.0	35.0	40.0	32.0	40.0
23	35.995437610046984	35.0	35.0	40.0	32.0	40.0
24	36.05270970942732	35.0	35.0	40.0	32.0	40.0
25	36.07898479527613	35.0	35.0	40.0	32.0	40.0
26	36.03192505617868	35.0	35.0	40.0	32.0	40.0
27	36.025773125674874	35.0	35.0	40.0	32.0	40.0
28	35.90943412745508	35.0	35.0	40.0	32.0	40.0
29	35.9105431092347	35.0	35.0	40.0	32.0	40.0
30	35.75735770499139	35.0	34.0	40.0	31.0	40.0
31	35.73691255581389	35.0	34.0	40.0	31.0	40.0
32	35.7125888887808	35.0	34.0	40.0	31.0	40.0
33	35.63192019222351	35.0	34.0	40.0	31.0	40.0
34	35.10116443086861	35.0	34.0	40.0	29.0	40.0
35	35.11324260435616	35.0	34.0	40.0	30.0	40.0
36	35.23676760994971	35.0	34.0	40.0	30.0	40.0
37	35.297878342753194	35.0	34.0	40.0	31.0	40.0
38	35.37451968442659	35.0	34.0	40.0	31.0	40.0
39	35.39198322908256	35.0	34.0	40.0	31.0	40.0
40	35.31264725624288	35.0	34.0	40.0	31.0	40.0
41	35.27283286477232	35.0	34.0	40.0	31.0	40.0
42	35.17912974114031	35.0	34.0	40.0	30.0	40.0
43	35.21390312946876	35.0	34.0	40.0	31.0	40.0
44	35.217928538770586	35.0	34.0	40.0	31.0	40.0
45	35.05908635466015	35.0	34.0	40.0	30.0	40.0
46	34.81599560298452	35.0	34.0	39.0	29.0	40.0
47	34.78843351459673	35.0	34.0	39.0	29.0	40.0
48	34.695580610329095	35.0	34.0	39.0	29.0	40.0
49	34.62268256855745	35.0	34.0	39.0	29.0	40.0
50	35.00520929599113	35.0	34.0	39.0	31.0	40.0
51	35.12934132319037	35.0	34.0	39.0	31.0	40.0
52	34.80104185919823	35.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	9.0
12	10.0
13	14.0
14	42.0
15	71.0
16	136.0
17	259.0
18	460.0
19	685.0
20	1097.0
21	1510.0
22	2094.0
23	3004.0
24	4106.0
25	5577.0
26	7492.0
27	9592.0
28	11977.0
29	15349.0
30	20194.0
31	28916.0
32	45464.0
33	91260.0
34	231637.0
35	76855.0
36	49624.0
37	98917.0
38	262724.0
39	58895.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.32926058153448	0.553809936087629	0.09776549899316127	0.019163983384729126
8	99.23499713026645	0.573946710507116	0.11887506444740606	0.07218109477903051
9	94.49497553430548	2.9293656429662347	1.913577244472115	0.6620815782561748
10	49.43510024611613	40.33833672188877	3.7602264657528917	6.466336566242205
11	39.783553994766386	20.354193215755323	25.416792318841992	14.445460470636304
12	33.85721373191824	17.279687150403223	23.598354037569187	25.264745080109343
13	26.278685175637424	17.122192281875932	28.36814303919375	28.230979503292897
14	26.792999795713886	18.093815967392043	29.896203196591344	25.216981040302734
15	29.42615056859636	21.37027345155987	26.750002431977588	22.45357354786618
16	35.21600824926798	23.77297002830822	22.27068883333171	18.740332889092095
17	30.84165880327247	23.421014231932837	24.46472173312451	21.272605231670187
18	31.39721976322266	23.151842952615347	23.62880239695711	21.822134887204882
19	29.18742764866679	26.841347510141343	22.691323676760994	21.27990116443087
20	31.16219344922517	24.870375594618523	22.997558294502756	20.96987266165355
21	29.099584618228157	26.095314065585573	25.230794672996293	19.574306643189978
22	27.893518293335408	20.35798710079088	29.190540579978013	22.557954025895697
23	28.32183818593928	22.097629308248294	27.79088883916846	21.78964366664397
24	30.779692014358396	22.288880025681685	24.523575590727358	22.407852369232565
25	26.716830257692347	23.636973841649077	24.019183439205424	25.627012461453152
26	24.471044874850435	25.792386937361982	26.36185880910922	23.374709378678364
27	23.432882282556882	22.86399408543051	25.6573635417376	28.045760090275007
28	23.11536328881193	27.239024485150342	24.02365827796531	25.621953948072413
29	29.23665087502554	24.398669221864452	22.90271116861387	23.461968734496143
30	27.09446773738533	21.762794634084653	26.374213255250638	24.768524373279377
31	29.06252127980389	22.00083660028989	25.117659075653958	23.818983044252263
32	30.956253587166945	22.839577030458088	24.340690876192888	21.863478506182087
33	31.215696956136853	22.199383250483965	25.213770829888034	21.37114896349115
34	26.56400478613189	23.953617323462748	28.920007393211865	20.5623704971935
35	23.950017996634145	28.839557574637393	26.44153039485588	20.768894033872584
36	24.947809760985244	29.93063999922177	23.880755274959387	21.240794964833604
37	29.684912983841944	27.07374728834499	21.819702909617984	21.421636818195083
38	27.205852310865104	29.341420469468954	21.86338122707861	21.589345992587333
39	29.42021654328434	24.27580571417454	23.384340009922468	22.919637732618657
40	26.733562263490178	23.207583878906973	22.67050594861718	27.388347908985672
41	23.44027549442104	27.544578149167776	23.290076558654434	25.725069797756746
42	23.039874704514723	24.14914832144907	23.305349377900132	29.505627596136076
43	22.57244861231359	23.272079924511416	25.903868789945232	28.251602673229765
44	24.963763533955273	22.436744262964872	23.89700088523984	28.70249131784001
45	26.36127513448836	23.66430926972577	23.86937361985272	26.10504197593315
46	24.844012957576584	26.957887876105335	22.492290631049546	25.705808535268538
47	23.72199577808691	24.646730935727696	25.33284045254239	26.298432833643005
48	25.900269463116626	22.742297926982307	27.504012763018377	23.85341984688269
49	27.59866533070031	22.58392754652373	25.338190803233555	24.4792163195424
50	24.27016352617294	22.507758008502194	25.512320398455206	27.709758066869654
51	23.04065293734253	23.409535297722698	27.45216300086578	26.097648764068992
52	22.70153798262595	23.42451627965797	27.548761150617235	26.325184587098843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	20.0
6	38.0
7	46.5
8	55.0
9	267.0
10	479.0
11	887.0
12	1295.0
13	1630.0
14	1732.0
15	1499.0
16	1382.0
17	1265.0
18	1487.5
19	1710.0
20	1994.5
21	2279.0
22	2701.5
23	3124.0
24	4070.5
25	5017.0
26	6313.5
27	7610.0
28	7538.0
29	7466.0
30	10238.0
31	13010.0
32	14440.0
33	15870.0
34	17558.0
35	19246.0
36	22293.5
37	25341.0
38	26990.5
39	32936.5
40	37233.0
41	38246.0
42	39259.0
43	40102.5
44	40946.0
45	43492.5
46	46039.0
47	50569.5
48	55100.0
49	55429.5
50	55759.0
51	62287.0
52	68815.0
53	69674.5
54	70534.0
55	74020.0
56	77506.0
57	82895.0
58	88284.0
59	88176.5
60	88069.0
61	86585.0
62	85101.0
63	69438.0
64	44589.0
65	35403.0
66	26875.0
67	18347.0
68	14537.5
69	10728.0
70	10242.0
71	9756.0
72	7634.0
73	5512.0
74	4514.5
75	3517.0
76	2309.0
77	1101.0
78	953.5
79	806.0
80	572.0
81	338.0
82	215.0
83	92.0
84	59.0
85	26.0
86	16.0
87	6.0
88	5.5
89	2.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1027970.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.746664887682918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.07504792618299	6.537580144617768
2	9.602612918750319	1.8718690033038825
3	3.398918848175883	0.9938436898079865
4	1.470957324397859	0.573477124199545
5	0.8879741588352381	0.43273932775445956
6	0.5829830335589326	0.3409284157982232
7	0.48598585643574366	0.3315718897982942
8	0.4069881554922955	0.3173421731871674
9	0.3909886211240448	0.3429751558493959
>10	10.708688346847559	27.712860418930724
>50	3.041911471763029	20.238263181861665
>100	1.8909449681472346	33.908537509635536
>500	0.04399871951268023	2.8592958644938125
>1k	0.0099997089801546	1.7565902909558573
>5k	9.9997089801546E-4	0.48741652583200584
>10k+	9.9997089801546E-4	1.2947092839736785
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	13284	1.292255610572293	No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5001	0.4864927964823876	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3540	0.3443680263042696	No Hit
TTTAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2561	0.24913178400147862	No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	2193	0.21333307392239076	No Hit
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1926	0.18735955329435686	No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1809	0.1759778981876903	No Hit
TTTAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1341	0.13045127776102414	No Hit
TTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1264	0.12296078679338893	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1223	0.11897234355088185	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	1151	0.11196824810062551	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0012646283451851709	0.0	0.0	0.0	0.0
8	0.0037938850355555124	0.0	0.0	0.0	0.0
9	0.009241514830199325	0.0	0.0	0.0	0.0
10	0.027335428076694847	0.0	0.0	0.0	0.0
11	0.0419272935980622	0.0	0.0	0.0	0.0
12	0.05087697111783418	0.0	0.0	0.0	0.0
13	0.06041032325846085	0.0	0.0	0.0	0.0
14	0.07023551270951486	0.0	0.0	0.0	0.0
15	0.0816171678161814	0.0	0.0	0.0	0.0
16	0.0937770557506542	0.0	0.0	0.0	0.0
17	0.10350496609823244	0.0	0.0	0.0	0.0
18	0.11430294658404429	0.0	0.0	0.0	0.0
19	0.12714378824284756	0.0	0.0	0.0	0.0
20	0.13940095528079613	0.0	0.0	0.0	0.0
21	0.1495179820422775	0.0	0.0	0.0	0.0
22	0.15564656556125178	0.0	0.0	0.0	0.0
23	0.16508263859840266	0.0	0.0	0.0	0.0
24	0.17189217584170743	0.0	0.0	0.0	0.0
25	0.1797717832232458	0.0	0.0	0.0	0.0
26	0.1876513906047842	0.0	0.0	0.0	0.0
27	0.19514188157241943	0.0	0.0	0.0	0.0
28	0.20224325612615154	0.0	0.0	0.0	0.0
29	0.21002558440421412	0.0	0.0	0.0	0.0
30	0.21702967985447047	0.0	0.0	0.0	0.0
31	0.22422833351167837	0.0	0.0	0.0	0.0
32	0.2312324289619347	0.0	0.0	0.0	0.0
33	0.2384310826191426	0.0	0.0	0.0	0.0
34	0.2466025273111083	0.0	0.0	0.0	0.0
35	0.256135879451735	0.0	0.0	0.0	0.0
36	0.27345155987042424	0.0	0.0	0.0	0.0
37	0.28444409856318764	0.0	0.0	0.0	0.0
38	0.29514479994552373	0.0	0.0	0.0	0.0
39	0.30253801180968315	0.0	0.0	0.0	0.0
40	0.3106121773981731	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	55	1.8189894E-12	46.000004	28
AATCCGT	35	1.0194344E-7	46.000004	30
CGCGACA	35	1.0194344E-7	46.000004	25
CGATGCG	35	1.0194344E-7	46.000004	21
TCACGCG	20	6.3111435E-4	46.0	39
CTCGTCA	20	6.3111435E-4	46.0	39
CGGCATA	30	1.86121E-6	46.0	37
CGGTTAG	40	5.6097633E-9	46.0	29
CGGTTAC	25	3.4169017E-5	46.0	29
CGCATAG	40	5.6097633E-9	46.0	23
TTCACGC	20	6.3111435E-4	46.0	42
CACGCGC	20	6.3111435E-4	46.0	40
ATTCGTC	20	6.3111435E-4	46.0	14
ATTCGAA	65	0.0	46.0	10
GCGTTAG	20	6.3111435E-4	46.0	16
ACGATAT	30	1.86121E-6	46.0	33
TACGCCT	25	3.4169017E-5	46.0	26
CGTAGTG	20	6.3111435E-4	46.0	26
TATTCGA	30	1.86121E-6	46.0	18
CATACCG	25	3.4169017E-5	46.0	31
>>END_MODULE