FastQCFastQC Report
Fri 17 Jun 2016
SRR1527331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325142
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC162411.2256045012534507No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT62420.47104385794126213No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC46500.35090578971913955No Hit
TTTAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC31130.23491822008509275No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT28470.21484489964094414No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT23070.17409454986710857No Hit
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA22440.16934034239349444No Hit
TTTAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG19130.1443618872543471No Hit
TTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT15780.11908157767243058No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT14600.11017687161074059No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC14260.10761110884720279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG351.01972546E-746.00000428
CATACCG351.01972546E-746.00000431
AATCCGT501.6370905E-1146.030
TCGCACT301.8616211E-646.011
ATTCGAA206.311806E-446.010
CGCGACA206.311806E-446.025
TAACGAA253.417441E-546.024
TATGACG206.311806E-446.037
CGTTACG405.6134013E-946.046
TACGCGG206.311806E-446.044
CGAATCG206.311806E-446.041
TCCCGTA600.046.037
CGTACAC405.6134013E-946.042
GATTGCG206.311806E-446.09
CCGAAAC253.417441E-546.023
CGGTATA206.311806E-446.016
TATCGTC405.6134013E-946.041
TATCGGG253.417441E-546.016
TTCGAAA206.311806E-446.031
TATCGAG453.110472E-1046.021
Produced by FastQC (version 0.11.5)