FastQCFastQC Report
Fri 17 Jun 2016
SRR1527330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9399
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC96610.277689115863389No Hit
CCTTTCTGGCCACACCTCTGTGAACCCCTATATTTTCTGGGACTGGAAGG8398.926481540589425No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACCTTAACTGGTTGATGCTGAAAA3764.000425577189063No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA3533.7557186934780296No Hit
CCTTTCGGGGCCTAACAGCGGGATAGACCTTCATTCAAGCCAATCAAAAT3403.6174061070326635No Hit
CCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGC2452.606660283008831No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCG2102.234280242578998No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC2042.1704436642195977No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGA1801.9150973507819982No Hit
CCTTTCGGGATAAGATCTTTCTATGACTATGTGCCTACAGTATCTTAACC1571.670390467070965No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1561.6597510373443984No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAG1501.5959144589849983No Hit
CCTTTCGGTTCTCAGTAAACCTGTTTTTGAAAAAAAAAAAAAAAAAAAAA1381.4682413022661984No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTC1251.329928715820832No Hit
CCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCA1201.2767315671879986No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGCTGTAGCGCC1131.2022555591020323No Hit
CCTTTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG740.7873177997659325No Hit
CCTTTCGGAGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC720.7660389403127992No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTTGCTACCCGTTGTAGCGC670.712841791679966No Hit
CCTTTCGGGGGGAATTCGTAGAGAAAGAAATGGCTCGGTGGGCTGCAATA650.6915629322268326No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT610.649005213320566No Hit
CCTTTCGGGGGGGTGATTGGCTGCCTGTTCAATGGTCATTGCGCTCGCCA570.6064474944142995No Hit
CCTTTCATCCCAAGTGATTCTAGGTTGTGTCAAGTTGACAGTTAAAGCTA540.5745292052345995No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT520.5532503457814661No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATT500.5319714863283328No Hit
CCTTTCGGGTAAGTAAGGATTGTAATAGTTTGTAAGGTTTATGTCAGTTT470.5000531971486328No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATT450.47877433769549954No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT380.404298329609533No Hit
CCTTTCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.3617406107032663No Hit
CCTTTCGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA330.3511011809766997No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT290.30854346207043304No Hit
CCTTTCGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC290.30854346207043304No Hit
CCTTTCGGGTGCCAAGTTCTTAAGCACGTTCATTTAACACAGCAATTCAA290.30854346207043304No Hit
CCTTTCGGGGGGAATTCGTAGAGAAAGAAATGGCTCGGGGGGCTGCAATA280.29790403234386637No Hit
CCTTTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAG260.2766251728907331No Hit
CCTTTCGGGCTGCGTGCGGAGCTTGTAGACGGCGCCGACGGAGATCCCCA220.23406745398446643No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTT210.22342802425789976No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTTT210.22342802425789976No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCCGAAAGGA180.1915097350781998No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGG180.1915097350781998No Hit
CCTTTCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1702308756250665No Hit
CCTTTCGGGAAGCAGTGATATCAACGCACCCTGCAATGCTCCTTTCGGGA160.1702308756250665No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGC160.1702308756250665No Hit
CCTTTCGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1702308756250665No Hit
CCTTTCGGGACGCAGATTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1702308756250665No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGT150.15959144589849983No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.15959144589849983No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTT140.14895201617193318No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTT140.14895201617193318No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG140.14895201617193318No Hit
CCTTTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAG130.13831258644536654No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTT130.13831258644536654No Hit
CCTTTCGGGGGCTGGAGCTCAACCGCTTTGAAAAGGCCGGTATGCTGCTC120.12767315671879986No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACCTTAACTGGTTGATGCTGAAAAA120.12767315671879986No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATT120.12767315671879986No Hit
CCTTTCGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.12767315671879986No Hit
CCTTTCGGGCACCGTGAGCGACTTGCGCGCGATGTCACCCGCCTTGAACA110.11703372699223322No Hit
CCTTTCGGGGGGAATTCGTAGAGAAAGAAATGGCTCCGGGGGCTGCAATA110.11703372699223322No Hit
CCTTTCGGGGCAGCAGCTCTTCGAGATGATTGAGATCGTTATGGCGGAAG110.11703372699223322No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTT110.11703372699223322No Hit
CCTTTCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCAT110.11703372699223322No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG110.11703372699223322Illumina Single End Adapter 2 (100% over 23bp)
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCCTT100.10639429726566656No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG100.10639429726566656No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCCTTT100.10639429726566656No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCAAA100.10639429726566656No Hit
CCTTTCGGGGGGAATTCGTAGAGAAAGAAATGGCTCGGTGGGGTGCAATA100.10639429726566656No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATT100.10639429726566656No Hit
CCTTTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA100.10639429726566656Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCGGGGAGTGCAGTGCTCCTTTCGGGGGTATCAACGCAAAGAAAAA100.10639429726566656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCTT453.8016879E-1044.00000425
GGCAACC453.8016879E-1044.00000423
AACCTTA453.8016879E-1044.00000426
GCAACCT453.8016879E-1044.00000424
CCTTAAC453.8016879E-1044.00000428
CTTAACT453.8016879E-1044.00000429
CTTCATT302.2682107E-644.029
CTTTCCT207.462225E-444.02
ACAGCGG351.2513738E-744.015
TGGTTTC207.462225E-444.029
TCCTTTA207.462225E-444.05
GCGGGAT351.2513738E-744.018
TAGCGCC850.044.044
TGTGAAC1000.044.019
TTCATTC302.2682107E-644.030
GGCACTG207.462225E-444.020
CTATATT1000.044.028
TGGCACT207.462225E-444.019
CCAACCA207.462225E-444.036
CCAATCA351.2513738E-744.040
Produced by FastQC (version 0.11.5)