FastQCFastQC Report
Fri 17 Jun 2016
SRR1527329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9763
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG8408.603912731742293No Hit
CCTTTCTGGCCACACCTCTGTGAACCCCTATATTTTCTGGGACTGGAAGGAA7848.03031854962614No Hit
CCTTTCGGGGCCTAACAGCGGGATAGACCTTCATTCAAGCCAATCAAAATAA3033.10355423537847No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACCTTAACTGGTTGATGCTGAAAAAC3033.10355423537847No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA2983.0523404691180986No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG2052.0997644166752023No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGAAG1581.618355013827717No Hit
CCTTTCGGGATAAGATCTTTCTATGACTATGTGCCTACAGTATCTTAACCAA1531.567141247567346No Hit
CCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAA1311.3418006760217145No Hit
CCTTTCGGTTCTCAGTAAACCTGTTTTTGAAAAAAAAAAAAAAAAAAAAAAA1211.2393731435009732No Hit
CCTTTCTGGCCACACATCTGTGAACCCCTATATTTTCTGGGACTGGAAGGAA1021.044760831711564No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTT1011.0345180784594898No Hit
CCTTTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC920.9423332991908225No Hit
CCTTTCGGGGGGAATACCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG920.9423332991908225No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGCTGTAGCGCCGG920.9423332991908225No Hit
CCTTTCGGGGGGGTGATTGGCTGCCTGTTCAATGGTCATTGCGCTCGCCATA800.8194202601659326No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.7989347536617843No Hit
CCTTTCGGAGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG740.7579637406534876No Hit
CCTTTCGGGGGGAATTCGTAGAGAAAGAAATGGCTCGGTGGGCTGCAATAAA730.7477209874014135No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGA710.7272354808972652No Hit
CCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATT660.6760217146368943No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT580.5940796886203011No Hit
CCTTTCGGGTAAGTAAGGATTGTAATAGTTTGTAAGGTTTATGTCAGTTTTC550.5633514288640786No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTTGCTACCCGTTGTAGCGCCG540.5531086756120045No Hit
CCTTTCGGGGGGAATTCGTAGAGAAAGAAATGGCTCGGGGGGCTGCAATAAA490.5018949093516337No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT460.47116664959541127No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA460.47116664959541127No Hit
CCTTTCATCCCAAGTGATTCTAGGTTGTGTCAAGTTGACAGTTAAAGCTAAA460.47116664959541127No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTG450.46092389634333714No Hit
CCTTTCGGGGCCTAAAAGCGGGATAGACCTTCATTCAAGCCAATCAAAATAA440.45068114309126295No Hit
CCTTTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGC430.44043838983918876No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT430.44043838983918876No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA410.41995288333504044No Hit
CCTTTCGGGGGGAATACCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA380.389224623578818No Hit
CCTTTCGGGGGGAATGCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG350.35849636382259553No Hit
CCTTTCTGGCCACACGTCTGTGAACCCCTATATTTTCTGGGACTGGAAGGAA320.327768104066373No Hit
CCTTTCGGGGGGGAAATCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG310.3175253508142989No Hit
CCTTTCGGGTGCCAAGTTCTTAAGCACGTTCATTTAACACAGCAATTCAATT300.30728259756222476No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCT300.30728259756222476No Hit
CCTTTCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.29703984431015057No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCC290.29703984431015057No Hit
CCTTTCGGGCTGCGTGCGGAGCTTGTAGACGGCGCCGACGGAGATCCCCAGT270.27655433780600225No Hit
CCTTTCGGGGGGGAAATCCTTCGGCAACCTTAACTGGTTGATGCTGAAAAAC260.2663115845539281No Hit
CCTTTCGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG260.2663115845539281No Hit
CCTTTCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT230.23558332479770563No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT210.21509781829355729No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTCCTGCACCCTCAACTGAGTGGCTAAT210.21509781829355729No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT200.20485506504148315No Hit
CCTTTCGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGAAG190.194612311789409No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG170.1741268052852607No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1741268052852607No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTT170.1741268052852607No Hit
CCTTTCGGGCACCGTGAGCGACTTGCGCGCGATGTCACCCGCCTTGAACACC160.1638840520331865No Hit
CCTTTCCCATCTTGGATTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATT160.1638840520331865No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT160.1638840520331865Illumina Single End Adapter 2 (95% over 24bp)
CCTTTCGGGGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGC160.1638840520331865No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTGAT150.15364129878111238No Hit
CCTTTCATCCCAAGTGATTTTAGGTTGTGTCAAGTTGACAGTTAAAGCTAAA150.15364129878111238No Hit
CCTTTCGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1433985455290382No Hit
CCTTTCGGGATAAGAACTTTCTATGACTATGTGCCTACAGTATCTTAACCAA140.1433985455290382No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACCTTAACTGGTTGATGCTGGAAAAC140.1433985455290382No Hit
CCTTTCGGGGGGGAAGTCCTTCGGCAACCTTAACTGGTTGATGCTGAAAAAC140.1433985455290382No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTAT140.1433985455290382No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA140.1433985455290382No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC130.13315579227696406Illumina Single End Adapter 2 (100% over 25bp)
CCTTTCGGGGGGGAATTCCTTCGGCAACCTTAACTGGTTGATGCTGAAAACC130.13315579227696406No Hit
CCTTTCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.13315579227696406No Hit
CCTTTCGCCATCAACAAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGA130.13315579227696406No Hit
CCTTTCGGGAAGCAATGGTATCAACCAGAGTGCGTGCTCCTTTCGGGGGAAA130.13315579227696406No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTT130.13315579227696406No Hit
CCTTTCGGGACGCAGATTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.13315579227696406No Hit
CCTTTCGGGAAGCAATGGTATCAACCAGAGTGCGTGCTCCTTTCGGGGAAAA120.12291303902488988No Hit
CCTTTCGGGGGGAATACCTTCGATTCTGTTTTTGCTACCCGTTGTAGCGCCG120.12291303902488988No Hit
CCTTTCCTTTATCAGAGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAA120.12291303902488988No Hit
CCTTTCGGGGTGGTAACAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.12291303902488988No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT120.12291303902488988No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGGAG110.11267028577281574No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTATGCCGTCTTCTGC110.11267028577281574Illumina Single End Adapter 1 (95% over 21bp)
CCTTTCGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.11267028577281574No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTT110.11267028577281574No Hit
CCTTTCGGGGGGAATACGTAGAGAAAGAAATGGCTCGGGGGGCTGCAATAAA110.11267028577281574No Hit
CCTTTCGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT100.10242753252074158No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG100.10242753252074158No Hit
CCTTTCGGGAAGCAGTGATATCAACGCACCCTGCAATGCTCCTTTCGGGAAA100.10242753252074158No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTG100.10242753252074158No Hit
CCTTTCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA100.10242753252074158Illumina Single End Adapter 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGG452.4738256E-1046.00000415
ATCTTTC206.0071185E-446.00000415
CACACCT900.046.00000411
ATAAGAT206.0071185E-446.00000410
GCCTAAC452.4738256E-1046.00000410
CCTAACA452.4738256E-1046.00000411
ACACCTC900.046.00000412
TAAGATC206.0071185E-446.00000411
CCTCTGT900.046.00000415
CACCTCT900.046.00000413
AAGATCT206.0071185E-446.00000412
GATCTTT206.0071185E-446.00000414
CTAACAG452.4738256E-1046.00000412
CCACACC900.046.00000410
TAACAGC452.4738256E-1046.00000413
ACCTCTG900.046.00000414
GCCACAC900.046.0000049
AACAGCG452.4738256E-1046.00000414
CAGCGGG452.4738256E-1046.00000416
AGATCTT206.0071185E-446.00000413
Produced by FastQC (version 0.11.5)