FastQCFastQC Report
Fri 17 Jun 2016
SRR1527328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9436
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG8749.262399321746503No Hit
CCTTTCTGGCCACACCTCTGTGAACCCCTATATTTTCTGGGACTGGAAGGAA7407.842306061890632No Hit
CCTTTCGGGGCCTAACAGCGGGATAGACCTTCATTCAAGCCAATCAAAATAA3503.7091988130563793No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA2973.147520135650699No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACCTTAACTGGTTGATGCTGAAAAAC2953.1263247138618055No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1932.045358202628232No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGAAG1551.6426451886392541No Hit
CCTTTCGGGATAAGATCTTTCTATGACTATGTGCCTACAGTATCTTAACCAA1381.4624841034336584No Hit
CCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAA1261.3353115727002967No Hit
CCTTTCGGTTCTCAGTAAACCTGTTTTTGAAAAAAAAAAAAAAAAAAAAAAA1231.3035184400169564No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1141.2081390419669353No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTT1001.05977108944468No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGCTGTAGCGCCGG940.9961848240779992No Hit
CCTTTCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC740.7842306061890632No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGA670.7100466299279355No Hit
CCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATT670.7100466299279355No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTTGCTACCCGTTGTAGCGCCG670.7100466299279355No Hit
CCTTTCGGGGGGAATTCGTAGAGAAAGAAATGGCTCGGTGGGCTGCAATAAA660.6994489190334888No Hit
CCTTTCGGAGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG650.688851208139042No Hit
CCTTTCGGGGGGGTGATTGGCTGCCTGTTCAATGGTCATTGCGCTCGCCATA610.6464603645612548No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT570.6040695209834677No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT540.5722763883001272No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT540.5722763883001272No Hit
CCTTTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGC530.5616786774056803No Hit
CCTTTCGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCATTGTAGCGCCGG460.4874947011445528No Hit
CCTTTCATCCCAAGTGATTCTAGGTTGTGTCAAGTTGACAGTTAAAGCTAAA430.4557015684612124No Hit
CCTTTCTGGCCACACCTCTGGGAACCCCTATATTTTCTGAGACTGGAAGGAA410.4345061466723188No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCT410.4345061466723188No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTG400.42390843577787196No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA380.4027130139889784No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA370.3921153030945316No Hit
CCTTTCGGGGGGAATTCGTAGAGAAAGAAATGGCTCGGGGGGCTGCAATAAA370.3921153030945316No Hit
CCTTTCGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCGTTGTAGCGCCGG350.370919881305638No Hit
CCTTTCGGGTAAGTAAGGATTGTAATAGTTTGTAAGGTTTATGTCAGTTTTC350.370919881305638No Hit
CCTTTCTGGCCACACCTCTGGGAACCCCTATATTTTCTGGGACTGGAAGGAA330.3497244595167444No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCC320.3391267486222976No Hit
CCTTTCGGGTGCCAAGTTCTTAAGCACGTTCATTTAACACAGCAATTCAATT250.26494277236117No Hit
CCTTTCGGGGGGAATTCCTTGGGCAACATTAACTGGTTGATGCTGAAAAACA250.26494277236117No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.2543450614667232No Hit
CCTTTCGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG240.2543450614667232No Hit
CCTTTCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT230.2437473505722764No Hit
CCTTTCGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.22255192878338279No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGGAG200.21195421788893598No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA190.2013565069944892No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTCCTGCACCCTCAACTGAGTGGCTAAT190.2013565069944892No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT180.19075879610004237No Hit
CCTTTCGGGGCCTAACAGCGGGATAGACCTTCATTCAAGACAATCAAAATAA170.1801610852055956No Hit
CCTTTCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1801610852055956No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT170.1801610852055956No Hit
CCTTTCGGGCTGCGTGCGGAGCTTGTAGACGGCGCCGACGGAGATCCCCAGT170.1801610852055956No Hit
CCTTTCTGGCCACACCTCTGGGAACCCCTATATTTTCTGTGACTGGAAGGAA160.1695633743111488No Hit
CCTTTCGGGGGGGAATTCCTGCGGCAACCTTAACTGGTTAATGCTGAAAAAC160.1695633743111488No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACCTTAACTGGTTGATGCTGAAAACC160.1695633743111488No Hit
CCTTTCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1695633743111488No Hit
CCTTTCGGGAAGCAATGGTATCAACCAGAGTGCGTGCTCCTTTCGGGGGAAA160.1695633743111488No Hit
CCTTTCGGGGGGGAATTCCTGCGGCAACCTTAACTGGTTGATGCTGAAAAAC150.15896566341670199No Hit
CCTTTCGGGCACCGTGAGCGACTTGCGCGCGATGTCACCCGCCTTGAACACC140.1483679525222552No Hit
CCTTTCGGGGGGAATTCCTTGGGCAACATTAACTGGTTGGTGCTGAAAAACA140.1483679525222552No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTT140.1483679525222552No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGC140.1483679525222552No Hit
CCTTTCTGGCCACACCTCTGAGAACCCCTATATTTTCTGAGACTGGAAGGAA140.1483679525222552No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG130.1377702416278084No Hit
CCTTTCGGGGGGGAATTCCTGCGATTCTGTTTTGCTACCAGTTGTAGCGCCG130.1377702416278084No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT130.1377702416278084No Hit
CCTTTCGGGAAGCAGTGATATCAACGCACCCTGCAATGCTCCTTTCGGGAAA120.1271725307333616No Hit
CCTTTCGGGAAGCAGTGATATCAATGCAGAATGCAGTGCTCTTTCGGGGAAA120.1271725307333616No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTT120.1271725307333616No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGAT120.1271725307333616No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGCAG110.11657481983891478No Hit
CCTTTCGGGTACAAGAAACGAGCAAAGCCACAAAAGCACCAAAAAACCCAAA110.11657481983891478No Hit
CCTTTCTGGCCACACCTCTGCGAACCCCTATATTTTCTGAGACTGGAAGGAA110.11657481983891478No Hit
CCTTTCGGGGGCTGGAGCTCAACCGCTTTGAAAAGGCCGGTATGCTGCTCTC110.11657481983891478No Hit
CCTTTCGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCCTTGTAGCGCCGG110.11657481983891478No Hit
CCTTTCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAA110.11657481983891478No Hit
CCTTTCATCCCAAGTGATTTTAGGTTGTGTCAAGTTGACAGTTAAAGCTAAA110.11657481983891478No Hit
CCTTTCGGGGCCTAACAGCGGGATAGACCTTCATTCAAGGCAATCAAAATAA110.11657481983891478No Hit
CCTTTCGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCGCTGTAGCGCCGG100.10597710894446799No Hit
CCTTTCGGGAAGCAATGGTATCAACCAGAGTGCGTGCTCCTTTCGGGGAAAA100.10597710894446799No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGTAG100.10597710894446799No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCCGAAAGGAGC100.10597710894446799No Hit
CCTTTCGGGGGGGAATTCCTTCGGCAACCTTAACTGGTTGATGCTGGAAAAC100.10597710894446799No Hit
CCTTTCGGGATAAGATCTTTGTATGACTATGTGCCTACAGTATCTTAACCAA100.10597710894446799No Hit
CCTTTCTGGCCACACCTCTGTGAACCCCTATATTTTCTGAGACTGGAAGGAA100.10597710894446799No Hit
CCTTTCGGGGGGAATTCCTTTGATTCTGTTTTGCTACCCATTGTAGCGCCGG100.10597710894446799No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTTAT100.10597710894446799No Hit
CCTTTCGGGGAGTCGACTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTT100.10597710894446799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATT750.046.00000428
TATATTT750.046.00000429
CCTATAT750.046.00000427
CACACCT750.046.00000411
TCTGGCC750.046.0000045
TTTTCTG750.046.00000433
ACACCTC750.046.00000412
TTCTGGC750.046.0000044
AACCCCT750.046.00000423
CTGGCCA750.046.0000046
CACCTCT750.046.00000413
GAACCCC750.046.00000422
ACCCCTA750.046.00000424
GCGCCGG1150.046.00000446
CTGGAAG750.046.00000443
TATTTTC750.046.00000431
CCACACC750.046.00000410
GGCCACA750.046.0000048
GGAAGGA750.046.00000445
ACCTCTG750.046.00000414
Produced by FastQC (version 0.11.5)