FastQCFastQC Report
Fri 17 Jun 2016
SRR1527327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3667075
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC404181.1021863474294908No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT336800.9184431733738743No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG195410.5328770205136246No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC194280.5297955454960698No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC139970.38169385682049045No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA133890.36511388504462006No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG121900.33241752623003346No Hit
CAACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT114690.31275607943660816No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC81420.2220298194064752No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG80130.2185120293421869No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT79830.2176939386295617No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG73010.19909600976254918No Hit
CAACAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC66480.18128890191774097No Hit
CAACAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT57770.15753700156118977No Hit
CAACAAGGGATATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTT57410.15655529270603954No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC56830.15497365066163088No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC56240.15336473892680133No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT55700.151892175644076No Hit
CAACAAGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGT55420.1511286243122925No Hit
CAACAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA55140.150365072980509No Hit
CAACAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC55100.15025599421882563No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG53470.1458110346802288No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC48950.13348513461000935No Hit
CAACAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG46480.126749521076062No Hit
CAACAAGGGGCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTGC43080.11747782633297656No Hit
CAACAAGGGGTATCTGTGGGTGTGCTGTAGGTGTCTGTGTGGGTGTGCTG42890.11695970221498059No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG41640.11355099091237565No Hit
CAACAAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC40000.10907876168335798No Hit
CAACAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG39720.10831521035157447No Hit
CAACAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC39670.10817886189947029No Hit
CAACAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA39310.10719715304432005No Hit
CAACAAGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGG38490.10496103842981123No Hit
CAACAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA38060.10378844174171512No Hit
CAACAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA36880.10057061827205606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA302.529241E-644.042
ATAGACG207.858742E-444.019
CGCGAAA207.858742E-444.028
TTATCCG207.858742E-444.017
CTAACGA700.044.038
TATTACG207.858742E-444.042
CGTACAC207.858742E-444.018
ACGTAGT254.444755E-544.044
ACGTAAG207.858742E-444.015
CGGTAAT207.858742E-444.036
TAATGCG254.444755E-544.042
TCGAAAC254.444755E-544.043
TACCGGA408.3218765E-944.024
CAAGGGA808650.042.683244
AACAAGG3764800.042.6191522
CAACAAG3775450.042.6154751
ACAAGGG3747350.042.5839583
AAGGGAT276500.042.4325525
CAAGGGG2659700.042.4217764
AAGGGGG1087650.042.2200135
Produced by FastQC (version 0.11.5)