##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527327_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3667075
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25677058691191	33.0	31.0	34.0	31.0	34.0
2	32.88160291240294	34.0	31.0	34.0	31.0	34.0
3	33.19509663696543	34.0	34.0	34.0	31.0	34.0
4	36.46684073819052	37.0	37.0	37.0	35.0	37.0
5	36.205662278518986	37.0	37.0	37.0	35.0	37.0
6	36.3547058623017	37.0	37.0	37.0	35.0	37.0
7	36.4695415283298	37.0	37.0	37.0	35.0	37.0
8	36.60971619069694	37.0	37.0	37.0	35.0	37.0
9	38.753410279309804	39.0	39.0	39.0	39.0	39.0
10	38.3172981190731	39.0	39.0	39.0	37.0	39.0
11	37.98835884185625	39.0	38.0	39.0	35.0	39.0
12	37.37877981770212	39.0	37.0	39.0	35.0	39.0
13	37.23617160816182	39.0	37.0	39.0	35.0	39.0
14	37.97851121125147	40.0	38.0	40.0	34.0	40.0
15	38.023335219486924	40.0	38.0	40.0	34.0	40.0
16	37.954057116366585	40.0	38.0	40.0	34.0	40.0
17	37.955982083813396	40.0	38.0	40.0	34.0	40.0
18	37.97462473497269	40.0	38.0	40.0	34.0	40.0
19	37.943657547227694	40.0	38.0	40.0	34.0	40.0
20	37.710192455840144	40.0	37.0	40.0	33.0	40.0
21	37.83611406911503	40.0	37.0	40.0	34.0	40.0
22	37.70870816659054	40.0	37.0	40.0	34.0	40.0
23	37.782673111403504	40.0	37.0	40.0	34.0	40.0
24	37.77658351683562	40.0	37.0	40.0	34.0	40.0
25	37.749571524989264	40.0	37.0	40.0	34.0	40.0
26	37.620889128256174	40.0	36.0	40.0	34.0	40.0
27	37.493175623623735	40.0	36.0	40.0	33.0	40.0
28	37.285398853309516	40.0	35.0	40.0	33.0	40.0
29	37.51292842388007	40.0	36.0	40.0	33.0	40.0
30	37.5280415590082	40.0	36.0	40.0	33.0	40.0
31	37.49026840192797	40.0	36.0	40.0	33.0	40.0
32	36.794163740856135	40.0	35.0	40.0	32.0	40.0
33	36.99971694061343	40.0	35.0	40.0	32.0	40.0
34	36.88731209478944	40.0	35.0	40.0	32.0	40.0
35	36.882843137923274	39.0	35.0	40.0	32.0	40.0
36	36.706754838665695	39.0	35.0	40.0	32.0	40.0
37	36.643720131167214	39.0	35.0	40.0	31.0	40.0
38	36.43431263336583	39.0	35.0	40.0	31.0	40.0
39	36.41380828044149	39.0	35.0	40.0	31.0	40.0
40	36.22605837077234	39.0	35.0	40.0	31.0	40.0
41	36.05090733077453	38.0	35.0	40.0	30.0	40.0
42	35.93480498762638	38.0	35.0	40.0	30.0	40.0
43	35.84028987680917	38.0	35.0	40.0	30.0	40.0
44	35.672906335430824	37.0	35.0	40.0	30.0	40.0
45	35.56322736786131	37.0	35.0	40.0	30.0	40.0
46	35.24087617515322	37.0	35.0	40.0	29.0	40.0
47	35.12704294294499	36.0	34.0	40.0	28.0	40.0
48	34.96992943967604	36.0	34.0	40.0	28.0	40.0
49	34.631288424698155	35.0	34.0	40.0	27.0	40.0
50	34.34209935711705	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	7.0
14	14.0
15	44.0
16	115.0
17	292.0
18	625.0
19	1211.0
20	2069.0
21	3215.0
22	5239.0
23	7844.0
24	10877.0
25	14603.0
26	19212.0
27	24955.0
28	33055.0
29	43080.0
30	57721.0
31	75593.0
32	93771.0
33	128009.0
34	249930.0
35	299334.0
36	324428.0
37	589960.0
38	1083678.0
39	598193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.6869984933496	0.2693427322866317	0.03149649243606962	0.012162281927694417
8	99.7161770620999	0.23893702746739567	0.03615960949803317	0.00872630093466864
9	99.3937402425639	0.412017752568464	0.06045690366300116	0.13378510120463857
10	70.23355126360929	21.816952203050118	2.8520823817347614	5.097414151605844
11	37.646353019777344	26.841938056898208	17.970207863215233	17.541501060109216
12	32.25273549082034	15.946987721821888	26.79844290067697	25.001833886680803
13	24.022988349024768	15.825773947901256	31.16170244677297	28.989535256301004
14	24.59698806269302	16.137384700340192	32.076546021011296	27.189081215955497
15	24.825644416871757	16.80235610125236	29.827096527886667	28.544902953989215
16	31.32979281852703	17.373737924640213	25.287102118173205	26.009367138659556
17	31.15870278082668	18.109201475290252	23.415119679853834	27.316976064029237
18	30.3531833954855	18.76329226972451	23.448525050619363	27.434999284170626
19	29.945910569050266	19.880613295337564	22.4187397312572	27.75473640435497
20	28.966792334490023	21.228417744387556	22.862581212546786	26.942208708575638
21	30.75650211680972	19.45261550418249	24.498708098416312	25.292174280591478
22	30.37775338655468	18.392560828453195	24.914325450120327	26.315360334871794
23	29.910241813979805	19.090228588179954	24.29039493329152	26.709134664548724
24	29.73680658290327	19.613370329213335	23.34972696222466	27.300096125658733
25	28.926651350190546	20.04845823987783	22.704989671604753	28.31990073832687
26	27.630141188822154	18.739240402773326	23.873741333351514	29.756877075053005
27	26.85690911693925	17.70991866814832	24.84724201168506	30.585930203227367
28	28.939495374378758	19.61893334605919	22.508784249026814	28.932787030535234
29	30.884642392097245	20.96245645371311	21.98209744823872	26.17080370595093
30	30.097775475003917	18.127717595086	24.59573365693366	27.17877327297642
31	31.24730200500399	19.83335492183825	22.3381305263732	26.58121254678456
32	34.05831623296496	19.638731141304717	21.715672572827117	24.5872800529032
33	35.12486109501442	18.487813857093187	21.344041231771914	25.043283816120475
34	29.57997859329302	20.594942835911457	26.625525793718424	23.1995527770771
35	29.465227736002124	23.54295998854673	25.230272083336175	21.76154019211497
36	30.517210583366854	22.875507045806263	22.652113741878747	23.95516862894814
37	30.4077500460176	24.12576781222091	20.44272342398233	25.023758717779153
38	31.648930005522114	23.683807939570368	21.42628661808117	23.240975436826353
39	32.27119707123525	21.854038982022455	20.575472276950975	25.29929166979132
40	29.730616363177738	21.509186476960522	21.37341068835516	27.38678647150658
41	27.67298187247329	22.253839913282384	22.942563214551107	27.130614999693215
42	26.23682362645978	20.41065426804742	23.32240273242298	30.03011937306982
43	26.843274271728827	18.860672334217327	26.446800242700245	27.849253151353597
44	26.350129190158366	19.023417846648897	24.343216323636685	30.283236639556048
45	28.68989589795682	19.24904726519092	23.663573829278103	28.39748300757416
46	27.93076225602149	21.93333924176626	23.374978695554358	26.760919806657895
47	26.5019668264216	20.383220959484056	24.320991525943704	28.79382068815064
48	28.37525820988117	20.554529154707772	25.71613615756427	25.35407647784679
49	29.330215498728553	19.64884819645085	25.23970739622178	25.781228908598813
50	26.26935636713184	20.31466495776607	25.157243852389165	28.258734822712928
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	7.0
6	11.0
7	7.0
8	3.0
9	296.5
10	590.0
11	2171.5
12	3753.0
13	5433.0
14	7113.0
15	6888.5
16	6664.0
17	7374.5
18	8085.0
19	8431.0
20	8777.0
21	8819.5
22	8862.0
23	12974.5
24	17087.0
25	16342.0
26	15597.0
27	18852.0
28	22107.0
29	27497.0
30	32887.0
31	33973.0
32	35059.0
33	34742.5
34	34426.0
35	38995.0
36	43564.0
37	50177.0
38	56790.0
39	62995.5
40	69201.0
41	70849.5
42	72498.0
43	79006.5
44	85515.0
45	91824.5
46	98134.0
47	104485.0
48	110836.0
49	123136.5
50	135437.0
51	148454.5
52	161472.0
53	185762.0
54	210052.0
55	270256.0
56	330460.0
57	345554.0
58	360648.0
59	373503.5
60	386359.0
61	392925.5
62	399492.0
63	349747.0
64	300002.0
65	253638.5
66	207275.0
67	174459.5
68	141644.0
69	126549.0
70	111454.0
71	94515.0
72	77576.0
73	69744.0
74	61912.0
75	46831.5
76	31751.0
77	19972.0
78	8193.0
79	5895.5
80	3598.0
81	2326.5
82	1055.0
83	818.0
84	581.0
85	447.5
86	314.0
87	269.5
88	225.0
89	117.0
90	9.0
91	6.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3667075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.198732558518134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.00768362100867	4.029652450153924
2	10.7771854294717	1.336097804217069
3	5.052590419568109	0.9395897021590089
4	2.7336062688550347	0.6777957672368391
5	1.6194455865433715	0.5019255042027446
6	1.0177522419792822	0.3785264375317081
7	0.6467522953666369	0.2806331157409857
8	0.4746347803811648	0.23537072532430642
9	0.35379602293234724	0.19737782337824725
>10	4.807623998287684	7.878889182380368
>50	3.535486920100602	16.04866170748952
>100	3.684548692844979	41.699166744827295
>500	0.18367364475855483	7.709886789624151
>1k	0.0950672633674932	10.863052381713326
>5k	0.006460881976431577	2.528682898731891
>10k+	0.003691932557960901	4.6946909652886255
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	40418	1.1021863474294908	No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	33680	0.9184431733738743	No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	19541	0.5328770205136246	No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	19428	0.5297955454960698	No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	13997	0.38169385682049045	No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	13389	0.36511388504462006	No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	12190	0.33241752623003346	No Hit
CAACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	11469	0.31275607943660816	No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	8142	0.2220298194064752	No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	8013	0.2185120293421869	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	7983	0.2176939386295617	No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	7301	0.19909600976254918	No Hit
CAACAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	6648	0.18128890191774097	No Hit
CAACAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	5777	0.15753700156118977	No Hit
CAACAAGGGATATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTT	5741	0.15655529270603954	No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	5683	0.15497365066163088	No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	5624	0.15336473892680133	No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	5570	0.151892175644076	No Hit
CAACAAGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGT	5542	0.1511286243122925	No Hit
CAACAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	5514	0.150365072980509	No Hit
CAACAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	5510	0.15025599421882563	No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	5347	0.1458110346802288	No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	4895	0.13348513461000935	No Hit
CAACAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	4648	0.126749521076062	No Hit
CAACAAGGGGCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTGC	4308	0.11747782633297656	No Hit
CAACAAGGGGTATCTGTGGGTGTGCTGTAGGTGTCTGTGTGGGTGTGCTG	4289	0.11695970221498059	No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	4164	0.11355099091237565	No Hit
CAACAAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	4000	0.10907876168335798	No Hit
CAACAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG	3972	0.10831521035157447	No Hit
CAACAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	3967	0.10817886189947029	No Hit
CAACAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA	3931	0.10719715304432005	No Hit
CAACAAGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGG	3849	0.10496103842981123	No Hit
CAACAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA	3806	0.10378844174171512	No Hit
CAACAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	3688	0.10057061827205606	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.01085333678749412	0.0	0.0	0.0	0.0
7	0.010935145858756639	0.0	0.0	0.0	0.0
8	0.011044224620439996	0.0	0.0	0.0	0.0
9	0.011235112453385872	0.0	0.0	0.0	0.0
10	0.014943790350620045	0.0	0.0	0.0	0.0
11	0.02211571893130083	0.0	0.0	0.0	0.0
12	0.024651800140438905	0.0	0.0	0.0	0.0
13	0.02819685989514804	0.0	0.0	0.0	0.0
14	0.035532406618353864	0.0	0.0	0.0	0.0
15	0.0411499628450468	0.0	0.0	0.0	0.0
16	0.05066708480191979	0.0	0.0	0.0	0.0
17	0.056884574217871194	0.0	0.0	0.0	0.0
18	0.06132953375646803	0.0	0.0	0.0	0.0
19	0.06825603512336126	0.0	0.0	0.0	0.0
20	0.0736008944458458	0.0	0.0	0.0	0.0
21	0.07938206881506378	0.0	0.0	0.0	0.0
22	0.08390883742492314	0.0	0.0	0.0	0.0
23	0.08783567284552402	0.0	0.0	0.0	0.0
24	0.09255332928832925	0.0	0.0	0.0	2.7269690420839497E-5
25	0.09708009789818861	0.0	0.0	0.0	2.7269690420839497E-5
26	0.1012796302229979	0.0	0.0	0.0	2.7269690420839497E-5
27	0.10586093821369894	0.0	0.0	0.0	2.7269690420839497E-5
28	0.11134214598828766	0.0	0.0	0.0	2.7269690420839497E-5
29	0.1159234539789887	0.0	0.0	0.0	2.7269690420839497E-5
30	0.11998663785169379	0.0	0.0	0.0	2.7269690420839497E-5
31	0.12672225138564114	0.0	0.0	0.0	2.7269690420839497E-5
32	0.13790282445818533	0.0	0.0	0.0	2.7269690420839497E-5
33	0.14286590811477812	0.0	0.0	0.0	2.7269690420839497E-5
34	0.14842892496062937	0.0	0.0	0.0	2.7269690420839497E-5
35	0.15652802301561872	0.0	0.0	0.0	2.7269690420839497E-5
36	0.16740862949353366	0.0	0.0	0.0	2.7269690420839497E-5
37	0.17348977045738087	0.0	0.0	0.0	2.7269690420839497E-5
38	0.17959818111164894	0.0	0.0	0.0	2.7269690420839497E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	30	2.529241E-6	44.0	42
ATAGACG	20	7.858742E-4	44.0	19
CGCGAAA	20	7.858742E-4	44.0	28
TTATCCG	20	7.858742E-4	44.0	17
CTAACGA	70	0.0	44.0	38
TATTACG	20	7.858742E-4	44.0	42
CGTACAC	20	7.858742E-4	44.0	18
ACGTAGT	25	4.444755E-5	44.0	44
ACGTAAG	20	7.858742E-4	44.0	15
CGGTAAT	20	7.858742E-4	44.0	36
TAATGCG	25	4.444755E-5	44.0	42
TCGAAAC	25	4.444755E-5	44.0	43
TACCGGA	40	8.3218765E-9	44.0	24
CAAGGGA	80865	0.0	42.68324	4
AACAAGG	376480	0.0	42.619152	2
CAACAAG	377545	0.0	42.615475	1
ACAAGGG	374735	0.0	42.583958	3
AAGGGAT	27650	0.0	42.432552	5
CAAGGGG	265970	0.0	42.421776	4
AAGGGGG	108765	0.0	42.220013	5
>>END_MODULE