FastQCFastQC Report
Fri 17 Jun 2016
SRR1527326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955662
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC389130.9837291457156856No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG323120.8168544228500817No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA195410.4940007513280963No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT182470.461288148481847No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT128770.32553337469177096No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC128170.32401656157679803No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG119050.3009610022292097No Hit
CAACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC106330.26880456419178383No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA81030.20484561117709246No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC77100.19491048527401986No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT70550.1783519421022322No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG68080.17210772811226036No Hit
CAACAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG61420.15527110253606097No Hit
CAACAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC59160.14955777313632965No Hit
CAACAAGGGATATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCT54440.13762550996520936No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT53750.1358811748829905No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC52420.1325189058114672No Hit
CAACAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC52330.1322913838442213No Hit
CAACAAGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCT51030.12900495542844662No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC50760.12832238952670882No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA49720.12569324679408908No Hit
CAACAAGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC48890.12359498865170987No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT46360.11719909335024074No Hit
CAACAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT46230.11687045050866327No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG45620.11532835717510748No Hit
CAACAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG45470.11494915389636425No Hit
CAACAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA41080.10385113793847907No Hit
CAACAAGGGGCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTGCCT39820.10066583039703594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGC206.3133315E-446.00000446
CGCTTAT301.8625651E-646.011
CAAGGGA874300.044.5031434
CAACAAG4080650.044.4646571
AACAAGG4068450.044.4577942
ACAAGGG4050000.044.4286163
AAGGGAT295050.044.394175
CAAGGGG2883850.044.227064
AAGGGGG1184700.044.0372245
AAGGGGT340150.043.9173935
AAGGGAC165550.043.6937525
AAGGGGA859600.043.6534425
AAGGGAG277250.043.569345
AGGGGAG409250.043.4878436
AGGGGGG494600.043.321476
AAGGGGC528850.043.320985
AGGGGGC292350.043.301526
AGGGGAT133950.043.0816
CAAGGGC203650.042.973244
CACGATA750.042.9333322
Produced by FastQC (version 0.11.5)