##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527326_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3955662
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22782305464926	33.0	31.0	34.0	31.0	34.0
2	32.85116852754356	34.0	31.0	34.0	31.0	34.0
3	33.16668284600656	34.0	34.0	34.0	31.0	34.0
4	36.447916682466804	37.0	37.0	37.0	35.0	37.0
5	36.24331123336625	37.0	37.0	37.0	35.0	37.0
6	36.39617464788448	37.0	37.0	37.0	35.0	37.0
7	36.500993765392494	37.0	37.0	37.0	35.0	37.0
8	36.57126518898733	37.0	37.0	37.0	35.0	37.0
9	38.717876047043454	39.0	39.0	39.0	39.0	39.0
10	38.20880145978094	39.0	39.0	39.0	37.0	39.0
11	37.88082095993035	39.0	38.0	39.0	35.0	39.0
12	37.30217470552337	39.0	37.0	39.0	35.0	39.0
13	37.19285899553602	39.0	37.0	39.0	34.0	39.0
14	37.9488497753347	40.0	38.0	40.0	34.0	40.0
15	37.89176501935707	40.0	38.0	40.0	33.0	40.0
16	32.8853744329015	40.0	34.0	40.0	3.0	40.0
17	34.51784833992389	40.0	34.0	40.0	17.0	40.0
18	36.60944716712399	40.0	34.0	40.0	30.0	40.0
19	37.495575961747996	40.0	36.0	40.0	33.0	40.0
20	37.74358274291383	40.0	37.0	40.0	33.0	40.0
21	37.91838079188768	40.0	37.0	40.0	34.0	40.0
22	37.98403149712993	40.0	37.0	40.0	34.0	40.0
23	37.972333834387264	40.0	37.0	40.0	34.0	40.0
24	37.92017063136335	40.0	37.0	40.0	34.0	40.0
25	37.89425006484376	40.0	37.0	40.0	34.0	40.0
26	37.79664693292804	40.0	37.0	40.0	34.0	40.0
27	37.68112214845454	40.0	36.0	40.0	34.0	40.0
28	37.55087618709587	40.0	36.0	40.0	33.0	40.0
29	37.363076016100464	40.0	36.0	40.0	33.0	40.0
30	37.3083787239658	40.0	35.0	40.0	33.0	40.0
31	37.26068278836766	40.0	35.0	40.0	33.0	40.0
32	37.19260796296549	39.0	35.0	40.0	33.0	40.0
33	36.870497782672025	39.0	35.0	40.0	32.0	40.0
34	36.65139488662075	39.0	35.0	40.0	31.0	40.0
35	36.59643543861937	39.0	35.0	40.0	31.0	40.0
36	36.55742806134599	39.0	35.0	40.0	31.0	40.0
37	36.26384332129489	38.0	35.0	40.0	31.0	40.0
38	36.24401882668438	38.0	35.0	40.0	31.0	40.0
39	35.90579882709898	38.0	35.0	40.0	30.0	40.0
40	35.79301669353954	38.0	35.0	40.0	30.0	40.0
41	35.70273547133198	38.0	34.0	40.0	30.0	40.0
42	35.633082149081496	37.0	34.0	40.0	30.0	40.0
43	35.53970005526256	37.0	34.0	40.0	30.0	40.0
44	35.768135649608084	37.0	35.0	40.0	30.0	40.0
45	35.83705458150873	37.0	35.0	40.0	31.0	40.0
46	35.74202674546005	37.0	35.0	40.0	31.0	40.0
47	35.42328363747964	36.0	35.0	40.0	30.0	40.0
48	35.343692408502044	36.0	34.0	40.0	30.0	40.0
49	35.22495147462043	36.0	34.0	40.0	30.0	40.0
50	34.764837592291755	35.0	34.0	40.0	28.0	40.0
51	34.80794061777776	35.0	34.0	39.0	29.0	40.0
52	34.48601649989306	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	10.0
15	32.0
16	111.0
17	239.0
18	596.0
19	1145.0
20	1953.0
21	3282.0
22	5094.0
23	7530.0
24	10963.0
25	15188.0
26	20331.0
27	27543.0
28	36524.0
29	48520.0
30	64535.0
31	86286.0
32	121978.0
33	189665.0
34	290388.0
35	356643.0
36	471837.0
37	766393.0
38	958392.0
39	470478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.71875756826543	0.2520437792713331	0.019642729838899278	0.00955592262432938
8	99.70543489307227	0.2401367963187957	0.04335557486964255	0.011072735739302295
9	99.40988385761979	0.4107277113160831	0.05129356350466749	0.12809486755946287
10	70.51269800099199	21.604854004209663	2.8345192283870566	5.047928766411286
11	37.655542864885824	26.94868772913358	17.774521685624304	17.62124772035629
12	32.274926421923816	15.898426104151467	26.81121390047987	25.015433573444852
13	24.075868969593458	15.736101820630783	31.14181646460188	29.04621274517388
14	24.67973755088276	16.08140432625437	32.05023583915916	27.18862228370371
15	24.88412811812536	16.964518202010183	29.735275663087496	28.416078016776964
16	29.506439124475246	25.718021408300306	22.33221645327634	22.443323013948106
17	31.254591519700114	17.98846817549123	23.37596083790779	27.38097946690086
18	30.368444017714353	18.71583062455791	23.415903583268744	27.499821774458994
19	30.026503781162294	19.894040491831706	22.352516468798395	27.7269392582076
20	28.88039979148876	21.231996060330736	22.857943878925955	27.029660269254553
21	30.90997157997827	19.44319307362459	24.33069357290891	25.316141773488233
22	30.132503737680317	18.36054243259409	24.985501794642715	26.521452035082877
23	30.042657840836757	19.051804729524413	24.156967910807346	26.748569518831488
24	29.872875892834124	19.45959993548488	23.310889555275452	27.356634616405547
25	28.986677830411196	19.96368749402755	22.685659189283612	28.363975486277642
26	27.68651618869357	18.642442150011806	23.82579704737159	29.84524461392303
27	26.91842730748987	17.654440647355614	24.838244521397428	30.588887523757087
28	29.012210851179905	19.65880805791799	22.33825842551765	28.990722665384457
29	31.125359042304424	20.855497764975876	21.859451085557865	26.159692107161835
30	30.16673315364154	18.062766737906323	24.51910704200713	27.25139306644501
31	31.4355220440978	19.773757211814356	22.223283991402702	26.567436752685136
32	34.25656691598018	19.231445962774373	21.704003021491726	24.807984099753718
33	35.33512216160026	18.41641171566226	21.30176946361949	24.946696659117993
34	29.68944262679673	20.46089883311567	26.574085450172436	23.275573089915163
35	29.47236138982552	23.58914892121723	25.222149920797076	21.71633976816017
36	30.557312530747065	22.85703379105697	22.612523516923336	23.97313016127263
37	30.665006261910143	24.034358850680366	20.399973506330927	24.900661381078564
38	31.630887573306314	23.66754287904275	21.325457028431654	23.376112519219287
39	32.463769654737945	21.797868473089967	20.57587326723062	25.162488604941473
40	29.716062696964503	21.491118300805276	21.30788727651655	27.484931725713675
41	27.604557720047872	22.2609515170912	22.96283150582633	27.1716592570346
42	26.272239640292828	20.361218931243368	23.261947052098993	30.10459437636482
43	26.880759781801377	18.771270143910172	26.39446949714106	27.953500577147388
44	26.380363135171812	18.959810014101304	24.36350729662949	30.296319554097394
45	28.698483338566337	19.15201551598696	23.684177262870286	28.46532388257642
46	27.68937285339344	21.87019517845559	23.402707309168477	27.037724658982494
47	26.607025574985933	20.416001164912473	24.261880817926304	28.715092442175294
48	28.40869619295076	20.570362179579547	25.704597612232792	25.31634401523689
49	29.4242531338623	19.434370277339166	25.343494969994907	25.79788161880363
50	26.26842232728681	20.47475239289909	25.05282301672893	28.204002263085165
51	25.93176565641857	21.658372226949623	24.02050529089695	28.389356825734858
52	27.577861809224345	21.675562775585984	23.791719312721867	26.954856102467804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	2.5
6	1.0
7	1.5
8	2.0
9	279.5
10	557.0
11	2206.5
12	3856.0
13	5572.5
14	7051.0
15	6813.0
16	7515.0
17	8217.0
18	8708.0
19	9199.0
20	8903.0
21	8607.0
22	9558.0
23	10509.0
24	15338.5
25	20168.0
26	20003.5
27	19839.0
28	21425.5
29	23012.0
30	30196.5
31	37381.0
32	36292.5
33	35204.0
34	36317.0
35	37430.0
36	42899.0
37	48368.0
38	53967.5
39	65645.0
40	71723.0
41	74929.0
42	78135.0
43	85049.5
44	91964.0
45	96491.5
46	101019.0
47	107926.5
48	114834.0
49	128019.0
50	141204.0
51	158267.0
52	175330.0
53	199712.5
54	224095.0
55	271764.5
56	319434.0
57	358079.0
58	396724.0
59	396793.0
60	396862.0
61	409899.0
62	422936.0
63	369621.5
64	283539.5
65	250772.0
66	206081.0
67	161390.0
68	143859.5
69	126329.0
70	110222.0
71	94115.0
72	84051.5
73	73988.0
74	56774.5
75	39561.0
76	27440.0
77	15319.0
78	10144.0
79	4969.0
80	3328.0
81	1687.0
82	1051.0
83	415.0
84	301.5
85	188.0
86	180.5
87	173.0
88	152.5
89	74.0
90	16.0
91	17.0
92	18.0
93	9.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3955662.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.2616409843744485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.92712970696327	4.932624290310036
2	10.511493492602494	1.5266138390573516
3	4.733591063736655	1.031209166150962
4	2.4659031233607274	0.7162601273637288
5	1.4981880609819187	0.5439651912963392
6	0.8680699402703637	0.3782167353342451
7	0.6013641801169326	0.30568235438102986
8	0.3918433623059285	0.2276340655340653
9	0.30288664009155986	0.19795086353775093
>10	4.257310056801668	8.366156542724513
>50	3.120311938186135	16.5508387573475
>100	3.0884595956912912	40.53146154948962
>500	0.1455527302930222	7.133603987552572
>1k	0.07983224542387368	10.810507553665854
>5k	0.004838317904477193	2.1433867321032527
>10k+	0.0032255452696514616	4.603888244151187
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	38913	0.9837291457156856	No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	32312	0.8168544228500817	No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	19541	0.4940007513280963	No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	18247	0.461288148481847	No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	12877	0.32553337469177096	No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	12817	0.32401656157679803	No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	11905	0.3009610022292097	No Hit
CAACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10633	0.26880456419178383	No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	8103	0.20484561117709246	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	7710	0.19491048527401986	No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	7055	0.1783519421022322	No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	6808	0.17210772811226036	No Hit
CAACAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	6142	0.15527110253606097	No Hit
CAACAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	5916	0.14955777313632965	No Hit
CAACAAGGGATATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCT	5444	0.13762550996520936	No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	5375	0.1358811748829905	No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5242	0.1325189058114672	No Hit
CAACAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	5233	0.1322913838442213	No Hit
CAACAAGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCT	5103	0.12900495542844662	No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	5076	0.12832238952670882	No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	4972	0.12569324679408908	No Hit
CAACAAGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4889	0.12359498865170987	No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	4636	0.11719909335024074	No Hit
CAACAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4623	0.11687045050866327	No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	4562	0.11532835717510748	No Hit
CAACAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	4547	0.11494915389636425	No Hit
CAACAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA	4108	0.10385113793847907	No Hit
CAACAAGGGGCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTGCCT	3982	0.10066583039703594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.010895774209222123	0.0	0.0	0.0	0.0
7	0.010921054427805004	0.0	0.0	0.0	0.0
8	0.010946334646387886	0.0	0.0	0.0	0.0
9	0.01099689508355365	0.0	0.0	0.0	0.0
10	0.014232763062162541	0.0	0.0	0.0	0.0
11	0.01981969136897945	0.0	0.0	0.0	0.0
12	0.021791548418444245	0.0	0.0	0.0	0.0
13	0.02497685595988737	0.0	0.0	0.0	0.0
14	0.031625553447185324	0.0	0.0	0.0	0.0
15	0.03658047628943019	0.0	0.0	0.0	0.0
16	0.0479060142145613	0.0	0.0	0.0	0.0
17	0.05417550842311603	0.0	0.0	0.0	0.0
18	0.058928189516697836	0.0	0.0	0.0	0.0
19	0.06577912875265884	0.0	0.0	0.0	0.0
20	0.0723014251470424	0.0	0.0	0.0	0.0
21	0.07864676001134577	0.0	0.0	0.0	0.0
22	0.08357640263500775	0.0	0.0	0.0	0.0
23	0.0879751606684292	0.0	0.0	0.0	0.0
24	0.09323344613366866	0.0	0.0	0.0	0.0
25	0.09811252832016487	0.0	0.0	0.0	0.0
26	0.10205624241909446	0.0	0.0	0.0	0.0
27	0.10678364329409337	0.0	0.0	0.0	0.0
28	0.11274977487965351	0.0	0.0	0.0	0.0
29	0.11709797247590921	0.0	0.0	0.0	0.0
30	0.12134504919783339	0.0	0.0	0.0	0.0
31	0.1269066972860674	0.0	0.0	0.0	0.0
32	0.13808055389970122	0.0	0.0	0.0	2.5280218582881957E-5
33	0.14265627346320287	0.0	0.0	0.0	2.5280218582881957E-5
34	0.148192641332854	0.0	0.0	0.0	2.5280218582881957E-5
35	0.1563581519351249	0.0	0.0	0.0	2.5280218582881957E-5
36	0.1663185580567804	0.0	0.0	0.0	2.5280218582881957E-5
37	0.17276501379541528	0.0	0.0	0.0	2.5280218582881957E-5
38	0.1789081069110556	0.0	0.0	0.0	2.5280218582881957E-5
39	0.18474783740370132	0.0	0.0	0.0	2.5280218582881957E-5
40	0.19111845248658757	0.0	0.0	0.0	2.5280218582881957E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGC	20	6.3133315E-4	46.000004	46
CGCTTAT	30	1.8625651E-6	46.0	11
CAAGGGA	87430	0.0	44.503143	4
CAACAAG	408065	0.0	44.464657	1
AACAAGG	406845	0.0	44.457794	2
ACAAGGG	405000	0.0	44.428616	3
AAGGGAT	29505	0.0	44.39417	5
CAAGGGG	288385	0.0	44.22706	4
AAGGGGG	118470	0.0	44.037224	5
AAGGGGT	34015	0.0	43.917393	5
AAGGGAC	16555	0.0	43.693752	5
AAGGGGA	85960	0.0	43.653442	5
AAGGGAG	27725	0.0	43.56934	5
AGGGGAG	40925	0.0	43.487843	6
AGGGGGG	49460	0.0	43.32147	6
AAGGGGC	52885	0.0	43.32098	5
AGGGGGC	29235	0.0	43.30152	6
AGGGGAT	13395	0.0	43.081	6
CAAGGGC	20365	0.0	42.97324	4
CACGATA	75	0.0	42.93333	22
>>END_MODULE