FastQCFastQC Report
Fri 17 Jun 2016
SRR1527325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3830568
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC364570.9517387499712836No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG308920.8064600341254873No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA187910.490553881304287No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT179720.4691732401043396No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT133910.34958262064529333No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC121810.3179946159420744No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG118250.30870095505418516No Hit
CAACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC106420.2778178066542612No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC71590.1868913435292103No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA71480.18660417985009012No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT70020.1827927346544951No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG66510.17362960271166053No Hit
CAACAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG60700.15846213929631323No Hit
CAACAAGGGATATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCT55590.1451220811117307No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT53230.13896111490515245No Hit
CAACAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC52750.13770803703262807No Hit
CAACAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC52530.1371337096743877No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC52280.1364810649491146No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC51140.13350500500186918No Hit
CAACAAGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCT49080.12812721246561867No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA47550.12413302674694718No Hit
CAACAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT46820.12222730414914967No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG45710.11932956156893704No Hit
CAACAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG43570.11374292272059913No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT40910.10679878284369315No Hit
CAACAAGGGGCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTGCCT39110.1020997408217267No Hit
CAACAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA38610.10079445137118047No Hit
CAACAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG38350.10011570085689643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGG3940900.044.4691542
CAACAAG3951150.044.469051
ACAAGGG3922250.044.4190673
CAAGGGA849800.044.367974
CAAGGGG2772100.044.2659344
AAGGGAT292050.044.117795
AAGGGGG1144700.044.1153145
AAGGGGT331200.043.847225
AAGGGGA820300.043.712065
AGGGGGG473400.043.6970836
AGGGGAG385900.043.6636436
AAGGGAG275000.043.3738175
AAGGGGC506250.043.1241465
CAAGGGC206800.043.0860754
AGGGATT96000.043.0531236
AGGGGGC287550.043.0005236
AAGGGAC155000.042.9729045
AGGGGAT132800.042.7959336
AGGGGGT214600.042.7525636
AGGGATC84150.042.6654786
Produced by FastQC (version 0.11.5)