##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3830568
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22501362722186	33.0	31.0	34.0	31.0	34.0
2	32.837180804517764	34.0	31.0	34.0	31.0	34.0
3	33.13939212148172	34.0	33.0	34.0	31.0	34.0
4	36.44621815876914	37.0	37.0	37.0	35.0	37.0
5	36.25964321740275	37.0	37.0	37.0	35.0	37.0
6	36.388183684508405	37.0	37.0	37.0	35.0	37.0
7	36.499169835909456	37.0	37.0	37.0	35.0	37.0
8	36.59464784334856	37.0	37.0	37.0	35.0	37.0
9	38.66680554946421	39.0	39.0	39.0	39.0	39.0
10	38.206520286286526	39.0	39.0	39.0	37.0	39.0
11	37.81563830742595	39.0	38.0	39.0	35.0	39.0
12	37.26890215759125	39.0	37.0	39.0	35.0	39.0
13	37.09845145680745	39.0	37.0	39.0	34.0	39.0
14	37.87889811641511	40.0	38.0	40.0	34.0	40.0
15	38.014355834434994	40.0	38.0	40.0	34.0	40.0
16	38.13459466063519	40.0	38.0	40.0	34.0	40.0
17	38.05533565779279	40.0	38.0	40.0	34.0	40.0
18	38.07077644881908	40.0	38.0	40.0	34.0	40.0
19	38.0355845399429	40.0	38.0	40.0	34.0	40.0
20	37.98639836180953	40.0	38.0	40.0	34.0	40.0
21	33.68335113748144	40.0	34.0	40.0	10.0	40.0
22	34.46425517051257	40.0	34.0	40.0	17.0	40.0
23	36.55133990572678	40.0	34.0	40.0	30.0	40.0
24	37.31278128987659	39.0	36.0	40.0	33.0	40.0
25	37.6186153071816	40.0	36.0	40.0	34.0	40.0
26	37.691112910670164	40.0	37.0	40.0	34.0	40.0
27	37.65197641707444	40.0	36.0	40.0	34.0	40.0
28	37.53013365119742	40.0	36.0	40.0	33.0	40.0
29	37.50785627614495	40.0	36.0	40.0	33.0	40.0
30	37.37689058124017	40.0	36.0	40.0	33.0	40.0
31	37.238851000687106	40.0	35.0	40.0	33.0	40.0
32	37.19987244711489	40.0	35.0	40.0	33.0	40.0
33	36.89675499821436	39.0	35.0	40.0	32.0	40.0
34	36.662772727177796	39.0	35.0	40.0	31.0	40.0
35	36.61089478113951	39.0	35.0	40.0	31.0	40.0
36	36.50458026068197	39.0	35.0	40.0	31.0	40.0
37	36.434434527725394	38.0	35.0	40.0	31.0	40.0
38	36.341532117430106	38.0	35.0	40.0	31.0	40.0
39	36.221435306722135	38.0	35.0	40.0	31.0	40.0
40	31.26402820678291	36.0	30.0	40.0	8.0	40.0
41	32.096889286393036	36.0	30.0	40.0	13.0	40.0
42	33.88943545709148	35.0	31.0	40.0	23.0	40.0
43	34.77807755925492	36.0	33.0	40.0	27.0	40.0
44	35.480216510971744	37.0	34.0	40.0	30.0	40.0
45	35.732442290542814	37.0	35.0	40.0	30.0	40.0
46	35.72908560819179	37.0	35.0	40.0	31.0	40.0
47	35.65770559353078	37.0	35.0	40.0	31.0	40.0
48	35.47626774932595	36.0	35.0	40.0	30.0	40.0
49	35.29033396613766	36.0	34.0	40.0	30.0	40.0
50	35.22115049256403	35.0	34.0	40.0	30.0	40.0
51	35.01677296943952	35.0	34.0	39.0	30.0	40.0
52	34.63945242585434	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	13.0
15	37.0
16	125.0
17	327.0
18	630.0
19	1196.0
20	2137.0
21	3315.0
22	5339.0
23	8079.0
24	11457.0
25	15707.0
26	21548.0
27	28671.0
28	38217.0
29	51253.0
30	69469.0
31	97074.0
32	143159.0
33	174543.0
34	321423.0
35	405179.0
36	487031.0
37	544420.0
38	920458.0
39	479757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.71792694973696	0.2531478360389373	0.020310303850499457	0.008614910373605168
8	99.7268551295787	0.23644013107194548	0.027828771085645784	0.008875968263714415
9	99.3531246540983	0.4163873347242498	0.09283218572284842	0.13765582545460622
10	70.50763750963304	21.61243450057537	2.83260863663039	5.047319353161202
11	37.759021638566395	26.901180190509606	17.784620975270506	17.55517719565349
12	32.31298856984134	15.926410913472886	26.7883770761934	24.972223440492375
13	24.233638457795294	15.736334663684342	31.09356105935203	28.936465819168333
14	24.687513705539228	16.078790403929652	32.008856127864064	27.224839762667052
15	24.898317951802447	16.780566224121333	29.779865544744275	28.54125027933194
16	31.14081253746181	17.303987293790374	25.391116930961676	26.164083237786144
17	31.25901955010327	18.02722207254903	23.34940927820626	27.36434909914143
18	30.38484109928345	18.71745913399788	23.358963996984258	27.538735769734412
19	29.98158497643169	19.871622171959878	22.37686421439327	27.76992863721516
20	28.90790608599038	21.181558452950057	22.82682359378557	27.08371186727399
21	35.23835107482755	18.904663746995222	23.160586106290243	22.69639907188699
22	30.214291979675078	18.35829568878558	24.961206797529766	26.46620553400958
23	29.978295647016317	19.09797711462112	24.144774352002106	26.778952886360457
24	29.92015805488899	19.473796053222394	23.20817695965716	27.39786893223146
25	29.008752748939582	19.9713201801926	22.68906334517492	28.3308637256929
26	27.71468878766804	18.604342750213547	23.807200394301837	29.873768067816574
27	26.914102556070013	17.661427756927957	24.817233371134513	30.607236315867514
28	29.01744597668022	19.623251695309936	22.34668592229664	29.0126164057132
29	31.010335803985207	20.89726641062109	21.82861653937484	26.26378124601887
30	30.194686532127875	18.096167461326885	24.50117058357925	27.20797542296599
31	31.46316682016871	19.76928747903705	22.21989010507058	26.54765559572366
32	34.262725527911265	19.20018127859889	21.693023071252096	24.844070122237746
33	35.339406584088835	18.464572355849054	21.266402267235566	24.929618792826545
34	29.69384696995328	20.43686471562442	26.581044899868633	23.288243414553662
35	29.50721668431418	23.557759580302452	25.186238698803937	21.74878503657943
36	30.62112459562133	22.853738662255832	22.61432247123664	23.910814270886195
37	30.581469902113735	24.04554624797158	20.366196344771843	25.006787505142842
38	31.6493010958166	23.650617871814312	21.3451112211035	23.354969811265587
39	32.32752949432042	21.80713147501885	20.535701232819783	25.32963779784095
40	29.828135148625478	26.1936088851575	19.707756134338304	24.27049983187872
41	27.63892978795834	22.21344197518488	22.95022565844021	27.19740257841657
42	26.246943012106822	20.392328239571782	23.23141111187688	30.12931763644452
43	26.847872169349294	18.774813552454884	26.41482934123608	27.96248493695974
44	26.344395922484605	18.970450335302754	24.32333794883683	30.36181579337581
45	28.635674918184456	19.15922129564075	23.672807792473595	28.532295993701197
46	27.64689205360667	21.850858671612148	23.434148669335723	27.068100605445462
47	26.425454397363524	20.408043924556356	24.249536883302948	28.916964794777172
48	28.404743108593816	20.58417968301307	25.706500968002654	25.304576240390457
49	29.406213386630913	19.472647398505913	25.315671200720104	25.805468014143074
50	26.274171350045215	20.343719260433442	25.126117066711778	28.25599232280957
51	25.965914193404217	21.684120997199372	24.003411504507948	28.346553304888467
52	27.66824658901761	21.691639464434516	23.71585623855261	26.924257707995263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	3.0
6	6.0
7	3.5
8	1.0
9	258.5
10	516.0
11	1975.5
12	3435.0
13	5147.5
14	6576.5
15	6293.0
16	7054.0
17	7815.0
18	8266.5
19	8718.0
20	8647.0
21	8576.0
22	9486.0
23	10396.0
24	15085.0
25	19774.0
26	19234.5
27	18695.0
28	20276.0
29	21857.0
30	28636.5
31	35416.0
32	34267.0
33	33118.0
34	34479.0
35	35840.0
36	41072.5
37	46305.0
38	51583.0
39	62677.5
40	68494.0
41	71874.0
42	75254.0
43	81851.0
44	88448.0
45	92706.5
46	96965.0
47	103521.5
48	110078.0
49	123085.0
50	136092.0
51	152695.5
52	169299.0
53	192263.0
54	215227.0
55	261600.5
56	307974.0
57	345536.5
58	383099.0
59	381522.0
60	379945.0
61	397594.5
62	415244.0
63	362194.5
64	277648.0
65	246151.0
66	202306.0
67	158461.0
68	141581.0
69	124701.0
70	107268.5
71	89836.0
72	81065.0
73	72294.0
74	56219.0
75	40144.0
76	27813.0
77	15482.0
78	10313.5
79	5145.0
80	3453.5
81	1762.0
82	1070.5
83	379.0
84	270.5
85	162.0
86	162.0
87	162.0
88	142.5
89	68.0
90	13.0
91	10.0
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3830568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.186845906443364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.3013455442394	4.836843997227472
2	10.637049241183478	1.528936675912719
3	4.7168274412037015	1.0169733596164363
4	2.543340042152285	0.7311437188254256
5	1.434314338057584	0.5154098064511085
6	0.8773670058206278	0.37833008845382693
7	0.6184492652106796	0.31112896990155947
8	0.42120052419864823	0.24216826105030836
9	0.316774167709573	0.2048946417423493
>10	4.520791446294934	8.864027655103513
>50	3.29839488325983	17.271158486000783
>100	3.083837424951568	39.98496451891575
>500	0.14218687188484586	6.953462579316582
>1k	0.0800333290848833	10.492883155144957
>5k	0.004682801169860192	2.012604501142249
>10k+	0.00340567357808014	4.655069585194951
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	36457	0.9517387499712836	No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	30892	0.8064600341254873	No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	18791	0.490553881304287	No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	17972	0.4691732401043396	No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	13391	0.34958262064529333	No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	12181	0.3179946159420744	No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	11825	0.30870095505418516	No Hit
CAACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10642	0.2778178066542612	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	7159	0.1868913435292103	No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	7148	0.18660417985009012	No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	7002	0.1827927346544951	No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	6651	0.17362960271166053	No Hit
CAACAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	6070	0.15846213929631323	No Hit
CAACAAGGGATATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCT	5559	0.1451220811117307	No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	5323	0.13896111490515245	No Hit
CAACAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	5275	0.13770803703262807	No Hit
CAACAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	5253	0.1371337096743877	No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5228	0.1364810649491146	No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	5114	0.13350500500186918	No Hit
CAACAAGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCT	4908	0.12812721246561867	No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	4755	0.12413302674694718	No Hit
CAACAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4682	0.12222730414914967	No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	4571	0.11932956156893704	No Hit
CAACAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	4357	0.11374292272059913	No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	4091	0.10679878284369315	No Hit
CAACAAGGGGCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTGCCT	3911	0.1020997408217267	No Hit
CAACAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	3861	0.10079445137118047	No Hit
CAACAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	3835	0.10011570085689643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.011799816632937988	0.0	0.0	0.0	0.0
7	0.011825922421948912	0.0	0.0	0.0	0.0
8	0.011904239788981686	0.0	0.0	0.0	0.0
9	0.01193034557799261	0.0	0.0	0.0	0.0
10	0.01566347340655485	0.0	0.0	0.0	0.0
11	0.02164169909005662	0.0	0.0	0.0	0.0
12	0.02412174904609447	0.0	0.0	0.0	0.0
13	0.027828771085645784	0.0	0.0	0.0	0.0
14	0.03380699676914755	0.0	0.0	0.0	0.0
15	0.03965469350759469	0.0	0.0	0.0	0.0
16	0.04952268175372425	0.0	0.0	0.0	0.0
17	0.05437835850975625	0.0	0.0	0.0	0.0
18	0.05855528475150421	0.0	0.0	0.0	0.0
19	0.06526447252731188	0.0	0.0	0.0	0.0
20	0.07090332295367162	0.0	0.0	0.0	0.0
21	0.07654217338003137	0.0	0.0	0.0	0.0
22	0.08116289803496506	0.0	0.0	0.0	0.0
23	0.08536593006572393	0.0	0.0	0.0	0.0
24	0.09053487628988703	0.0	0.0	0.0	0.0
25	0.09526002410086441	0.0	0.0	0.0	0.0
26	0.09922810403052498	0.0	0.0	0.0	0.0
27	0.1037966171074368	0.0	0.0	0.0	0.0
28	0.11003590068104782	0.0	0.0	0.0	0.0
29	0.11405619218873024	0.0	0.0	0.0	0.0
30	0.11854638789860929	0.0	0.0	0.0	0.0
31	0.1239241804348598	0.0	0.0	0.0	0.0
32	0.1352018812875793	0.0	0.0	0.0	0.0
33	0.13919606700625076	0.0	0.0	0.0	0.0
34	0.14467828269854496	0.0	0.0	0.0	0.0
35	0.1531365583380846	0.0	0.0	0.0	0.0
36	0.16216916133586456	0.0	0.0	0.0	0.0
37	0.1678080117622243	0.0	0.0	0.0	0.0
38	0.173107486931442	0.0	0.0	0.0	0.0
39	0.17838085631164882	0.0	0.0	0.0	0.0
40	0.18668249721712288	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAAGG	394090	0.0	44.469154	2
CAACAAG	395115	0.0	44.46905	1
ACAAGGG	392225	0.0	44.419067	3
CAAGGGA	84980	0.0	44.36797	4
CAAGGGG	277210	0.0	44.265934	4
AAGGGAT	29205	0.0	44.11779	5
AAGGGGG	114470	0.0	44.115314	5
AAGGGGT	33120	0.0	43.84722	5
AAGGGGA	82030	0.0	43.71206	5
AGGGGGG	47340	0.0	43.697083	6
AGGGGAG	38590	0.0	43.663643	6
AAGGGAG	27500	0.0	43.373817	5
AAGGGGC	50625	0.0	43.124146	5
CAAGGGC	20680	0.0	43.086075	4
AGGGATT	9600	0.0	43.053123	6
AGGGGGC	28755	0.0	43.000523	6
AAGGGAC	15500	0.0	42.972904	5
AGGGGAT	13280	0.0	42.795933	6
AGGGGGT	21460	0.0	42.752563	6
AGGGATC	8415	0.0	42.665478	6
>>END_MODULE