SRR1527321_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	955110
Sequences flagged as poor quality	0
Sequence length	50
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3465	0.3627854383264755	No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3108	0.3254075446807174	No Hit
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3094	0.3239417449299034	No Hit
ACGTTGGGGGTGGGGTCCCTTGGCACGGTACTGTCTGCAGCCCCTTGTCT	2541	0.2660426547727487	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2522	0.26405335511092964	No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2355	0.24656845808336214	No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2030	0.21254096386803617	No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2014	0.2108657641528201	No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1783	0.1866800682643884	No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1536	0.16081917266074067	No Hit
ACGTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1499	0.15694527331930355	No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1376	0.14406717550858014	No Hit
ACGTTGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	1350	0.14134497597135406	No Hit
ACGTTGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	1347	0.14103087602475106	No Hit
ACGTTGGGGTGGTTCACTTTTTATAGAAATTAATGTAGTGAAACACTAAA	1261	0.13202667755546482	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1258	0.1317125776088618	No Hit
ACGTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTC	1252	0.1310843777156558	No Hit
ACGTTGGGGACTATCCATCTATCTATCTATCCATCATCTATCTACCTACC	1248	0.13066557778685178	No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1187	0.12427887887259059	No Hit
ACGTTGGGGGAGACGTGCCGTGGTCTCAGCAATGCACCTGTTTTATACAT	1176	0.12312717906837956	No Hit
ACGTTGGGGATTTGTGGTTATACTGTTGGCATGTATCTACTTGAGCTATT	1170	0.12249897917517355	No Hit
ACGTTGGGGAGGCCTCTTGCTCCTAACAGCTCAGCCCTGGCCTTTTCTCA	1131	0.11841567986933443	No Hit
ACGTTGGGGATAGCCAAGTCCATGTTTGATGATGACACAGATGACATTTT	1053	0.11024908125765619	No Hit
ACGTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1049	0.10983028132885218	No Hit
ACGTTGGGGATCTCCTACCTTCCCTCTCTTATTTCTTTCCTTTCTTCCTT	1037	0.10857388154244013	No Hit
ACGTTGGGGAGCAGAAGATAGTCCTACTGAGAAATGAGCACTTTGATCAT	1017	0.10647988189842009	No Hit
ACGTTGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	1017	0.10647988189842009	No Hit
ACGTTGGGGATGCTAAAGAAACAGCCCTTCACCGGAAGGTTCTCACCACA	1011	0.10585168200521405	No Hit
ACGTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA	1005	0.10522348211200803	No Hit
ACGTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1001	0.10480468218320403	No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	997	0.10438588225440001	No Hit
ACGTTGGGGGTGTTGCTCACTTCATGTGTAACAGGTGTCCTCATGTTGTG	982	0.10281538252138497	No Hit
ACGTTGGGGGTCATCACACATTGTTAGAATCCGAGTCCTACTGGGAAGGT	981	0.10271068253918396	No Hit
ACGTTGGGGAGCTCTTCACCATTTACTCCTGATGTTTCTTGTATGGGGCC	974	0.10197778266377694	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	20	7.855811E-4	44.000004	15
AACGTAG	40	8.309144E-9	44.000004	16
GGTACGA	20	7.855811E-4	44.000004	12
CAAACGG	20	7.855811E-4	44.000004	20
ATAGGTT	20	7.855811E-4	44.000004	39
TAGGTCG	20	7.855811E-4	44.000004	16
TAGCGTC	20	7.855811E-4	44.000004	35
CTATACG	20	7.855811E-4	44.000004	21
CCAATCG	20	7.855811E-4	44.000004	36
CGATTTA	40	8.309144E-9	44.000004	20
TCGATTG	20	7.855811E-4	44.000004	38
ACGGGTA	40	8.309144E-9	44.000004	30
CGTTAAG	20	7.855811E-4	44.000004	27
AAACGTA	40	8.309144E-9	44.000004	15
AACGGAC	20	7.855811E-4	44.000004	21
GTAGCGT	20	7.855811E-4	44.000004	34
TACGCGT	20	7.855811E-4	44.000004	27
CCATCGA	20	7.855811E-4	44.000004	27
CGGATGT	20	7.855811E-4	44.000004	44
ATATACG	20	7.855811E-4	44.000004	10

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content