##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	955110
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23366837327637	33.0	31.0	34.0	31.0	34.0
2	32.80473243919548	34.0	31.0	34.0	31.0	34.0
3	32.963083833275746	34.0	31.0	34.0	31.0	34.0
4	35.62133995037221	35.0	35.0	37.0	35.0	37.0
5	36.20085225785512	37.0	35.0	37.0	35.0	37.0
6	36.20002512799573	37.0	37.0	37.0	35.0	37.0
7	36.60979887133419	37.0	37.0	37.0	35.0	37.0
8	36.674283590371786	37.0	37.0	37.0	35.0	37.0
9	38.6668299986389	39.0	39.0	39.0	39.0	39.0
10	38.10029630094963	39.0	39.0	39.0	37.0	39.0
11	36.9976170284051	39.0	35.0	39.0	34.0	39.0
12	36.69136643946771	39.0	35.0	39.0	33.0	39.0
13	36.61859052883961	39.0	35.0	39.0	33.0	39.0
14	37.43888452638963	40.0	36.0	40.0	33.0	40.0
15	37.47906628555873	40.0	36.0	40.0	33.0	40.0
16	37.38642878830711	40.0	35.0	40.0	33.0	40.0
17	37.454235637779945	40.0	35.0	40.0	33.0	40.0
18	37.46942865219713	40.0	35.0	40.0	33.0	40.0
19	37.38300091088985	40.0	35.0	40.0	33.0	40.0
20	37.09415983499283	39.0	35.0	40.0	33.0	40.0
21	37.285735674424934	39.0	35.0	40.0	33.0	40.0
22	37.131698966611175	39.0	35.0	40.0	33.0	40.0
23	37.17486153427354	39.0	35.0	40.0	33.0	40.0
24	37.133950016228496	39.0	35.0	40.0	33.0	40.0
25	37.07749264482625	39.0	35.0	40.0	33.0	40.0
26	36.93811393452063	39.0	35.0	40.0	33.0	40.0
27	36.815863094303275	39.0	35.0	40.0	32.0	40.0
28	36.63356367329418	38.0	35.0	40.0	31.0	40.0
29	36.95787919716054	39.0	35.0	40.0	33.0	40.0
30	37.04496550135587	39.0	35.0	40.0	33.0	40.0
31	37.02572792662625	39.0	35.0	40.0	33.0	40.0
32	36.257382919244904	39.0	35.0	40.0	31.0	40.0
33	36.50188459967962	39.0	35.0	40.0	31.0	40.0
34	36.44699039901163	39.0	35.0	40.0	31.0	40.0
35	36.4464417711049	39.0	35.0	40.0	31.0	40.0
36	36.22405691491033	39.0	35.0	40.0	31.0	40.0
37	36.157997508140426	38.0	35.0	40.0	31.0	40.0
38	35.94783323386835	38.0	35.0	40.0	30.0	40.0
39	35.87570855712954	38.0	35.0	40.0	30.0	40.0
40	35.7461800211494	38.0	35.0	40.0	30.0	40.0
41	35.61147093004994	38.0	35.0	40.0	30.0	40.0
42	35.49838029127535	37.0	35.0	40.0	30.0	40.0
43	35.39566856173634	37.0	35.0	40.0	29.0	40.0
44	35.27638910701385	37.0	34.0	40.0	29.0	40.0
45	35.11897582477411	36.0	34.0	40.0	29.0	40.0
46	34.79395043502843	36.0	34.0	40.0	27.0	40.0
47	34.70654270188774	36.0	34.0	40.0	27.0	40.0
48	34.52144674435405	35.0	34.0	40.0	27.0	40.0
49	34.282432389986496	35.0	33.0	40.0	26.0	40.0
50	33.82537299368659	35.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	6.0
15	13.0
16	48.0
17	131.0
18	225.0
19	393.0
20	628.0
21	1051.0
22	1607.0
23	2428.0
24	3604.0
25	4883.0
26	6375.0
27	8452.0
28	11268.0
29	14266.0
30	18201.0
31	23232.0
32	29948.0
33	45059.0
34	90579.0
35	81897.0
36	90483.0
37	149597.0
38	269202.0
39	101532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.4576540921988	0.45125692328632305	0.06459988901801886	0.026489095496853767
8	99.23171153060903	0.6071551967836165	0.06376228916041084	0.09737098344693282
9	94.54659672707855	2.500549674906555	1.8374846876276032	1.1153689103872853
10	52.08363434578216	33.053470280910055	6.233941640229921	8.628953733077866
11	33.10100407282931	22.63969595125169	25.254054506810736	19.00524546910827
12	29.85499052465161	17.212467673880496	27.76936687920763	25.16317492226026
13	24.070107108081793	17.598601208237792	30.492822816220123	27.838468867460293
14	24.414151249594287	18.878139690716253	31.08647171529981	25.62123734438965
15	24.796096784663547	18.701720220707564	28.49378605605637	28.00839693857252
16	29.236213629843682	19.549580676571285	25.750123022479087	25.46408267110595
17	31.151804504193237	20.14071677607815	23.0167205871575	25.69075813257112
18	28.980745673273233	19.11800734993875	25.35718398927872	26.544062987509292
19	28.24836929777722	19.95518840761797	23.929809131932448	27.86663316267236
20	27.39244694328402	22.659798347834283	24.59109421951398	25.356660489367716
21	30.10501408214761	18.278104092722305	27.387421344138374	24.22946048099172
22	30.733632775282427	18.762550910366347	24.75704369130257	25.746772623048653
23	28.80484970317555	20.4486394237313	24.04068641308331	26.705824460009843
24	27.412025839955607	20.43953052527981	26.693051062181322	25.455392572583264
25	27.575881312100176	21.98762446210384	24.34138476196459	26.09510946383139
26	27.669273696223474	21.119138109746523	24.563453424212916	26.64813476981709
27	29.206374134916395	18.97289317460816	24.363999958120008	27.456732732355437
28	29.65700285830951	20.98428453267163	23.522002701259538	25.836709907759314
29	29.853524724900797	20.330014343897563	23.865837442807635	25.950623488394008
30	28.455151762624197	19.729455245992607	24.172608390656574	27.642784600726618
31	31.07317481756028	21.67247751567882	21.752049502151586	25.50229816460931
32	29.960842206656825	21.56987153312184	22.82438671985426	25.644899540367078
33	32.23628691983122	20.07234768770089	23.106657871868162	24.58470752059972
34	28.57252044267152	22.587136560186785	26.249437237595668	22.590905759546022
35	28.99603187067458	23.966349425720598	24.331124163708893	22.706494539895928
36	28.73176911559925	22.905843306006638	23.992210321324244	24.370177257069866
37	29.68966925275623	23.726167666551497	22.072012647757848	24.512150432934426
38	30.371475536849157	22.36192689847243	23.14424516547832	24.122352399200093
39	29.8325847284606	23.10257457256232	23.459915611814345	23.604925087162734
40	28.751033912324235	23.442326014804575	22.491021976526266	25.31561809634492
41	26.61578247531698	22.52379307095518	24.831694778611887	26.02872967511595
42	27.25036906743726	20.90397964632346	24.413627749683283	27.432023536556
43	26.011872977981593	20.880526850310435	26.816806441142905	26.290793730565063
44	26.65138046926532	20.99286993121211	24.89053616860885	27.465213430913714
45	26.937211420674057	21.42172105830742	25.364512988032793	26.27655453298573
46	27.88296635989572	21.593743129063668	24.7495052925841	25.773785218456513
47	25.888955198877618	21.697814911371466	26.04820387180534	26.365026017945574
48	27.40878014050738	22.86103171362461	25.364617688014995	24.36557045785302
49	26.553904785836185	22.44977018353907	25.180031619394626	25.816293411230117
50	25.54637685711593	23.678738574614442	25.020678246484696	25.754206321784924
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	28.0
10	55.0
11	398.0
12	741.0
13	1085.5
14	1430.0
15	1872.5
16	2315.0
17	2383.0
18	2451.0
19	2376.0
20	2301.0
21	2723.5
22	3146.0
23	3462.5
24	3779.0
25	4101.5
26	4424.0
27	5430.5
28	6437.0
29	6023.5
30	5610.0
31	7900.0
32	10190.0
33	11490.0
34	12790.0
35	12545.5
36	12301.0
37	14387.0
38	16473.0
39	18819.0
40	21165.0
41	23358.5
42	25552.0
43	25818.0
44	26084.0
45	26961.0
46	27838.0
47	30477.5
48	33117.0
49	37067.0
50	41017.0
51	42788.5
52	44560.0
53	47923.0
54	51286.0
55	57187.5
56	63089.0
57	71388.0
58	79687.0
59	84849.5
60	90012.0
61	87042.0
62	84072.0
63	83207.5
64	82343.0
65	71970.5
66	61598.0
67	51854.0
68	42110.0
69	35943.5
70	29777.0
71	27630.0
72	25483.0
73	21054.0
74	16625.0
75	16742.5
76	16860.0
77	10958.0
78	5056.0
79	3468.0
80	1880.0
81	1355.5
82	831.0
83	674.0
84	517.0
85	302.0
86	87.0
87	49.5
88	12.0
89	9.5
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	955110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.549019509523033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69272604180846	7.457389461964044
2	11.285145902063364	2.380944485773607
3	4.2735101690326855	1.3524402644181264
4	2.1186370197475113	0.8939817301965693
5	1.220788150947475	0.6439059010669732
6	0.7618677240829832	0.48221744910163805
7	0.44692240357779467	0.33002152082095576
8	0.3139454915104288	0.2649453691896249
9	0.23395937902251002	0.2221237848369917
>10	3.4803957195606445	10.05590023453187
>50	2.616545704708475	20.169704532632345
>100	2.442575910050747	44.953382043174024
>500	0.0829855917045485	5.741678347081517
>1k	0.029994792182366926	5.05136487521171
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3465	0.3627854383264755	No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3108	0.3254075446807174	No Hit
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3094	0.3239417449299034	No Hit
ACGTTGGGGGTGGGGTCCCTTGGCACGGTACTGTCTGCAGCCCCTTGTCT	2541	0.2660426547727487	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2522	0.26405335511092964	No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2355	0.24656845808336214	No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2030	0.21254096386803617	No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2014	0.2108657641528201	No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1783	0.1866800682643884	No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1536	0.16081917266074067	No Hit
ACGTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1499	0.15694527331930355	No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1376	0.14406717550858014	No Hit
ACGTTGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	1350	0.14134497597135406	No Hit
ACGTTGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	1347	0.14103087602475106	No Hit
ACGTTGGGGTGGTTCACTTTTTATAGAAATTAATGTAGTGAAACACTAAA	1261	0.13202667755546482	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1258	0.1317125776088618	No Hit
ACGTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTC	1252	0.1310843777156558	No Hit
ACGTTGGGGACTATCCATCTATCTATCTATCCATCATCTATCTACCTACC	1248	0.13066557778685178	No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1187	0.12427887887259059	No Hit
ACGTTGGGGGAGACGTGCCGTGGTCTCAGCAATGCACCTGTTTTATACAT	1176	0.12312717906837956	No Hit
ACGTTGGGGATTTGTGGTTATACTGTTGGCATGTATCTACTTGAGCTATT	1170	0.12249897917517355	No Hit
ACGTTGGGGAGGCCTCTTGCTCCTAACAGCTCAGCCCTGGCCTTTTCTCA	1131	0.11841567986933443	No Hit
ACGTTGGGGATAGCCAAGTCCATGTTTGATGATGACACAGATGACATTTT	1053	0.11024908125765619	No Hit
ACGTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1049	0.10983028132885218	No Hit
ACGTTGGGGATCTCCTACCTTCCCTCTCTTATTTCTTTCCTTTCTTCCTT	1037	0.10857388154244013	No Hit
ACGTTGGGGAGCAGAAGATAGTCCTACTGAGAAATGAGCACTTTGATCAT	1017	0.10647988189842009	No Hit
ACGTTGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	1017	0.10647988189842009	No Hit
ACGTTGGGGATGCTAAAGAAACAGCCCTTCACCGGAAGGTTCTCACCACA	1011	0.10585168200521405	No Hit
ACGTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA	1005	0.10522348211200803	No Hit
ACGTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1001	0.10480468218320403	No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	997	0.10438588225440001	No Hit
ACGTTGGGGGTGTTGCTCACTTCATGTGTAACAGGTGTCCTCATGTTGTG	982	0.10281538252138497	No Hit
ACGTTGGGGGTCATCACACATTGTTAGAATCCGAGTCCTACTGGGAAGGT	981	0.10271068253918396	No Hit
ACGTTGGGGAGCTCTTCACCATTTACTCCTGATGTTTCTTGTATGGGGCC	974	0.10197778266377694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0014657997508140423	0.0	0.0	0.0	0.0
8	0.0016751997152160483	0.0	0.0	0.0	0.0
9	0.0033503994304320966	0.0	0.0	0.0	0.0
10	0.009108898451487263	0.0	0.0	0.0	0.0
11	0.016751997152160483	0.0	0.0	0.0	0.0
12	0.02251049617321565	0.0	0.0	0.0	0.0
13	0.027850195265466806	0.0	0.0	0.0	0.0
14	0.043031692684612245	0.0	0.0	0.0	0.0
15	0.050988891331888476	0.0	0.0	0.0	0.0
16	0.06114478960538577	0.0	0.0	0.0	0.0
17	0.06805498843065197	0.0	0.0	0.0	0.0
18	0.07559338714912418	0.0	0.0	0.0	0.0
19	0.08449288563620944	0.0	0.0	0.0	0.0
20	0.09108898451487263	0.0	0.0	0.0	0.0
21	0.09778978337573682	0.0	0.0	0.0	0.0
22	0.10459528221880202	0.0	0.0	0.0	0.0
23	0.11265718084827926	0.0	0.0	0.0	0.0
24	0.11925327972694244	0.0	0.0	0.0	0.0
25	0.12522117871239963	0.0	0.0	0.0	0.0
26	0.1311890776978568	0.0	0.0	0.0	0.0
27	0.14050737611374606	0.0	0.0	0.0	0.0
28	0.15160557422705237	0.0	0.0	0.0	0.0
29	0.15736407324810756	0.0	0.0	0.0	0.0
30	0.16448367203777575	0.0	0.0	0.0	0.0
31	0.17097507093423794	0.0	0.0	0.0	0.0
32	0.18060746929673022	0.0	0.0	0.0	0.0
33	0.18898346787281045	0.0	0.0	0.0	0.0
34	0.19788296635989572	0.0	0.0	0.0	0.0
35	0.2118080639926291	0.0	0.0	0.0	0.0
36	0.22363916198134245	0.0	0.0	0.0	0.0
37	0.23096816073541268	0.0	0.0	0.0	0.0
38	0.23829715948948288	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	20	7.855811E-4	44.000004	15
AACGTAG	40	8.309144E-9	44.000004	16
GGTACGA	20	7.855811E-4	44.000004	12
CAAACGG	20	7.855811E-4	44.000004	20
ATAGGTT	20	7.855811E-4	44.000004	39
TAGGTCG	20	7.855811E-4	44.000004	16
TAGCGTC	20	7.855811E-4	44.000004	35
CTATACG	20	7.855811E-4	44.000004	21
CCAATCG	20	7.855811E-4	44.000004	36
CGATTTA	40	8.309144E-9	44.000004	20
TCGATTG	20	7.855811E-4	44.000004	38
ACGGGTA	40	8.309144E-9	44.000004	30
CGTTAAG	20	7.855811E-4	44.000004	27
AAACGTA	40	8.309144E-9	44.000004	15
AACGGAC	20	7.855811E-4	44.000004	21
GTAGCGT	20	7.855811E-4	44.000004	34
TACGCGT	20	7.855811E-4	44.000004	27
CCATCGA	20	7.855811E-4	44.000004	27
CGGATGT	20	7.855811E-4	44.000004	44
ATATACG	20	7.855811E-4	44.000004	10
>>END_MODULE