FastQCFastQC Report
Fri 17 Jun 2016
SRR1527320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020224
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA32220.3158129979298664No Hit
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT31740.3111081487986952No Hit
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG29370.2878779562135374No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC24880.2438680132990402No Hit
ACGTTGGGGGTGGGGTCCCTTGGCACGGTACTGTCTGCAGCCCCTTGTCTCT23090.22632284674738096No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG22850.22397042218179536No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC20440.20034815883570667No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG18890.18515541684963302No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC16950.1661399849444828No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG16250.15927874662819147No Hit
ACGTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT13990.13712674863559376No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC13660.13389216485791355No Hit
ACGTTGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG13270.13006947493883694No Hit
ACGTTGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG12880.12624678501976036No Hit
ACGTTGGGGTGGTTCACTTTTTATAGAAATTAATGTAGTGAAACACTAAAGA12410.1216399535788219No Hit
ACGTTGGGGACTATCCATCTATCTATCTATCCATCATCTATCTACCTACCTA12180.11938554670346904No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT12180.11938554670346904No Hit
ACGTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG11700.11468069757229786No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC11020.10801549463647199No Hit
ACGTTGGGGGAGACGTGCCGTGGTCTCAGCAATGCACCTGTTTTATACATGA10950.10732937080484287No Hit
ACGTTGGGGATGCTAAAGAAACAGCCCTTCACCGGAAGGTTCTCACCACATC10650.10438884009786085No Hit
ACGTTGGGGGTCATCACACATTGTTAGAATCCGAGTCCTACTGGGAAGGTGC10360.10154632708111161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATA351.0194162E-746.00000430
TCTACTA351.0194162E-746.00000413
ATATACG351.0194162E-746.00000410
AGCGATT351.0194162E-746.00000411
ACCACGA351.0194162E-746.00000418
AACGTTA253.4168843E-546.044
AGCGTAT206.311119E-446.023
TCGTTAA206.311119E-446.035
CGTATTC253.4168843E-546.020
CGTATAT253.4168843E-546.034
CAACGTC206.311119E-446.024
CGAAATA253.4168843E-546.038
CCGATTC206.311119E-446.039
ACGCATT301.8611972E-646.044
GCGAAAC301.8611972E-646.09
GCAACGT453.092282E-1046.014
GACGTAT253.4168843E-546.042
CGCATAT551.8189894E-1246.022
CGCGCAA206.311119E-446.045
CGATTGG501.6370905E-1146.023
Produced by FastQC (version 0.11.5)