##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527320_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1020224
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15242436955022	33.0	31.0	34.0	31.0	34.0
2	32.74928937174581	34.0	31.0	34.0	31.0	34.0
3	32.94638334326579	34.0	31.0	34.0	31.0	34.0
4	35.57785447117496	35.0	35.0	37.0	35.0	37.0
5	36.06726660027602	37.0	35.0	37.0	35.0	37.0
6	36.13728945800138	37.0	35.0	37.0	35.0	37.0
7	36.60120032463459	37.0	37.0	37.0	35.0	37.0
8	36.642028613324136	37.0	37.0	37.0	35.0	37.0
9	38.636971880685024	39.0	39.0	39.0	38.0	39.0
10	38.02762040493068	39.0	38.0	39.0	35.0	39.0
11	36.99129504893043	39.0	35.0	39.0	34.0	39.0
12	36.71638189260398	39.0	35.0	39.0	33.0	39.0
13	36.71025480678753	39.0	35.0	39.0	33.0	39.0
14	37.54368256382912	40.0	36.0	40.0	33.0	40.0
15	37.48047095539803	40.0	36.0	40.0	33.0	40.0
16	32.497240802019945	39.0	33.0	40.0	3.0	40.0
17	34.0964876340882	39.0	34.0	40.0	17.0	40.0
18	36.1586073254501	39.0	34.0	40.0	30.0	40.0
19	37.04654370020701	39.0	35.0	40.0	32.0	40.0
20	37.296351585534154	39.0	35.0	40.0	33.0	40.0
21	37.50639957499529	40.0	35.0	40.0	33.0	40.0
22	37.56862806599335	40.0	35.0	40.0	33.0	40.0
23	37.522943980929675	40.0	35.0	40.0	33.0	40.0
24	37.42074289567781	40.0	35.0	40.0	33.0	40.0
25	37.38248267047236	40.0	35.0	40.0	33.0	40.0
26	37.27929944796437	40.0	35.0	40.0	33.0	40.0
27	37.154844426322065	39.0	35.0	40.0	33.0	40.0
28	37.04720826014679	39.0	35.0	40.0	33.0	40.0
29	36.840467387554106	39.0	35.0	40.0	32.0	40.0
30	36.82852589235305	39.0	35.0	40.0	32.0	40.0
31	36.794644117370304	39.0	35.0	40.0	32.0	40.0
32	36.65400637507057	39.0	35.0	40.0	32.0	40.0
33	36.371834028605484	38.0	35.0	40.0	31.0	40.0
34	36.26219928172637	38.0	35.0	40.0	31.0	40.0
35	36.153843665704784	38.0	35.0	40.0	31.0	40.0
36	36.06827422213161	38.0	35.0	40.0	31.0	40.0
37	35.764213545260645	38.0	35.0	40.0	30.0	40.0
38	35.72887522740104	38.0	34.0	40.0	30.0	40.0
39	35.297319020136754	37.0	34.0	40.0	29.0	40.0
40	35.26135044852895	37.0	34.0	40.0	29.0	40.0
41	35.19843093281475	37.0	34.0	40.0	29.0	40.0
42	35.13549671444702	36.0	34.0	40.0	29.0	40.0
43	35.025403244777614	36.0	34.0	40.0	29.0	40.0
44	35.37577139922213	36.0	34.0	40.0	30.0	40.0
45	35.416094896806975	36.0	34.0	40.0	30.0	40.0
46	35.31340176275014	36.0	34.0	40.0	30.0	40.0
47	35.01766768866445	36.0	34.0	40.0	29.0	40.0
48	34.92127807226648	35.0	34.0	40.0	29.0	40.0
49	34.88884793927608	35.0	34.0	40.0	29.0	40.0
50	34.42259837055392	35.0	34.0	39.0	27.0	40.0
51	34.46704449218995	35.0	34.0	39.0	28.0	40.0
52	34.1179231227652	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	14.0
16	38.0
17	83.0
18	241.0
19	348.0
20	598.0
21	1043.0
22	1580.0
23	2408.0
24	3512.0
25	4947.0
26	6782.0
27	9128.0
28	11759.0
29	15050.0
30	20414.0
31	27588.0
32	39449.0
33	60885.0
34	97126.0
35	95689.0
36	122613.0
37	184178.0
38	231172.0
39	83577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.47305689730884	0.44941110971708176	0.05949673797126905	0.01803525500282291
8	99.25575167806285	0.5873219998745374	0.07635578069129915	0.08057054137130669
9	94.59932328586663	2.440248416034126	1.8448889655605043	1.1155393325387366
10	52.470829935386746	32.68684132112163	6.149629885201681	8.692698858289944
11	33.212314158459314	22.73422307258014	24.857482278401605	19.195980490558938
12	29.98959052129728	17.116633209961734	27.855451351859983	25.038324916881
13	24.24938052819773	17.50066652029358	30.372741672417035	27.87721127909165
14	24.58479706417414	18.88340207640675	30.93261871902641	25.599182140392696
15	24.883947054764445	18.625811586475127	28.43846057336428	28.051780785396147
16	27.68313625243084	27.49670660560818	22.774214290195093	22.045942851765886
17	31.25254845994605	20.063535066808857	22.98995122639734	25.69396524684775
18	29.298565805156514	18.99563233172323	25.24053541183113	26.465266451289125
19	28.407290947870273	19.89582679882065	23.7150860987391	27.981796154569977
20	27.28508641239571	22.55710510632959	24.62008343265793	25.53772504861677
21	30.053890126089954	18.230800294837213	27.465929050875104	24.24938052819773
22	30.608670252807222	18.641886487673297	24.795143027413587	25.954300232105894
23	28.928156953767015	20.351315005332165	24.06696960667461	26.65355843422621
24	27.508762781506807	20.272802835455746	26.650029797377833	25.56840458565962
25	27.79134778244778	21.983015494636472	24.170280252179914	26.05535647073584
26	27.80526629446082	21.03283200552036	24.389153754469607	26.772747945549213
27	29.144187943039963	18.909768835079355	24.304466470108522	27.641576751772163
28	29.758954896179663	20.77690781632269	23.44494934445769	26.019187943039963
29	30.01605529766012	20.31426431842419	23.67421272191205	25.99546766200364
30	28.632241546954397	19.55972413901261	24.09304231227652	27.714992001756478
31	31.200010977981307	21.550267392258952	21.705331378207138	25.544390251552603
32	30.097998086694687	21.16123517972524	22.849785929364533	25.890980804215545
33	32.537952449658114	20.030307069819962	23.038175929991844	24.39356455053008
34	28.686347311962862	22.53269870146164	26.019383978420425	22.761570008155072
35	29.029507245467663	23.89730098488175	24.387585471425883	22.6856062982247
36	28.885323223135313	22.68737061664889	24.00551251489869	24.42179364531711
37	29.776598080421557	23.6393184241892	22.059175239947308	24.524908255441943
38	30.413615049244086	22.181893388118688	23.08424424440123	24.320247318235996
39	30.052223825356002	22.973778307508937	23.39192177404178	23.58207609309328
40	28.861308889028294	23.51493397528386	22.401453014240012	25.22230412144784
41	26.72070055203563	22.456146885389874	24.737998714007905	26.085153848566588
42	27.22735399284863	20.907467379712692	24.37886189699517	27.486316730443512
43	26.102208926667085	20.900312088325702	26.639835957593625	26.357643027413584
44	26.69472586412396	20.76573379963616	24.806219026409888	27.73332130983
45	26.8616499905903	21.274445611944042	25.479698575998995	26.38420582146666
46	27.648928078539615	21.615253120883256	24.763875384229344	25.97194341634778
47	26.23404272003011	21.679356690295464	25.831484066244276	26.25511652343015
48	27.510135029170062	22.869977573552475	25.193584938209646	24.426302459067813
49	26.598668527695878	22.42193871149865	25.03342403236936	25.945968728436107
50	25.5548781444075	23.673624615770656	24.827684900570855	25.94381233925099
51	26.55936343391255	22.606800075277587	24.32867683959601	26.50515965121385
52	26.531428392196222	22.619640392698074	25.39853992848629	25.45039128661941
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	36.5
10	73.0
11	408.5
12	744.0
13	1077.0
14	1842.0
15	2274.0
16	2348.5
17	2423.0
18	2404.5
19	2386.0
20	2745.5
21	3105.0
22	3278.0
23	3451.0
24	3606.5
25	3762.0
26	4974.0
27	6186.0
28	5705.0
29	5224.0
30	5910.5
31	6597.0
32	9665.5
33	12734.0
34	13377.5
35	14021.0
36	14054.0
37	14087.0
38	14794.0
39	19572.5
40	23644.0
41	22570.0
42	21496.0
43	25595.5
44	29695.0
45	31523.5
46	33352.0
47	33226.0
48	33100.0
49	35272.0
50	37444.0
51	41571.0
52	45698.0
53	50043.5
54	54389.0
55	61779.0
56	69169.0
57	75931.0
58	82693.0
59	88910.5
60	95128.0
61	89250.5
62	83373.0
63	84425.0
64	75510.5
65	65544.0
66	57109.5
67	48675.0
68	42424.0
69	36173.0
70	32124.0
71	28075.0
72	24817.5
73	21560.0
74	20534.5
75	19509.0
76	13755.5
77	8002.0
78	5403.0
79	2804.0
80	1756.0
81	708.0
82	495.0
83	282.0
84	251.0
85	220.0
86	120.0
87	20.0
88	12.0
89	5.0
90	6.0
91	5.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1020224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.42516009516678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.08561559092881	8.708249940856073
2	10.163107648761697	2.525564792005562
3	4.150049327090207	1.5469508187580492
4	2.2150464654541073	1.1008922780600239
5	1.4617049738133703	0.9080959155766344
6	1.0193752567055652	0.7599540456971027
7	0.7604969720652941	0.66145076408606
8	0.585682736962872	0.5821761417391311
9	0.44922530747111816	0.5023526727716248
>10	4.972552418436108	14.359402528104068
>50	2.226956018659519	20.091145135501336
>100	1.832790388699719	39.440417311204804
>500	0.05984296001413971	5.026851069569803
>1k	0.017553934937480982	3.786496586069728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3222	0.3158129979298664	No Hit
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3174	0.3111081487986952	No Hit
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2937	0.2878779562135374	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2488	0.2438680132990402	No Hit
ACGTTGGGGGTGGGGTCCCTTGGCACGGTACTGTCTGCAGCCCCTTGTCTCT	2309	0.22632284674738096	No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2285	0.22397042218179536	No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2044	0.20034815883570667	No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1889	0.18515541684963302	No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1695	0.1661399849444828	No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1625	0.15927874662819147	No Hit
ACGTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1399	0.13712674863559376	No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1366	0.13389216485791355	No Hit
ACGTTGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1327	0.13006947493883694	No Hit
ACGTTGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1288	0.12624678501976036	No Hit
ACGTTGGGGTGGTTCACTTTTTATAGAAATTAATGTAGTGAAACACTAAAGA	1241	0.1216399535788219	No Hit
ACGTTGGGGACTATCCATCTATCTATCTATCCATCATCTATCTACCTACCTA	1218	0.11938554670346904	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1218	0.11938554670346904	No Hit
ACGTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG	1170	0.11468069757229786	No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1102	0.10801549463647199	No Hit
ACGTTGGGGGAGACGTGCCGTGGTCTCAGCAATGCACCTGTTTTATACATGA	1095	0.10732937080484287	No Hit
ACGTTGGGGATGCTAAAGAAACAGCCCTTCACCGGAAGGTTCTCACCACATC	1065	0.10438884009786085	No Hit
ACGTTGGGGGTCATCACACATTGTTAGAATCCGAGTCCTACTGGGAAGGTGC	1036	0.10154632708111161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0016663007339564645	0.0	0.0	0.0	0.0
8	0.0016663007339564645	0.0	0.0	0.0	0.0
9	0.0036266545386111283	0.0	0.0	0.0	0.0
10	0.009997804403738786	0.0	0.0	0.0	0.0
11	0.01656498964933191	0.0	0.0	0.0	0.0
12	0.021367856470735837	0.0	0.0	0.0	0.0
13	0.0259746879116743	0.0	0.0	0.0	0.0
14	0.038226899190765946	0.0	0.0	0.0	0.0
15	0.04557822595822094	0.0	0.0	0.0	0.0
16	0.05783043723731259	0.0	0.0	0.0	0.0
17	0.06547581707546578	0.0	0.0	0.0	0.0
18	0.07312119691361897	0.0	0.0	0.0	0.0
19	0.08155071827363403	0.0	0.0	0.0	0.0
20	0.08831393889969262	0.0	0.0	0.0	0.0
21	0.09458707107458754	0.0	0.0	0.0	0.0
22	0.100664167869017	0.0	0.0	0.0	0.0
23	0.10899567153879933	0.0	0.0	0.0	0.0
24	0.11605294523555611	0.0	0.0	0.0	0.0
25	0.12154193588858918	0.0	0.0	0.0	0.0
26	0.1263448027099931	0.0	0.0	0.0	0.0
27	0.13232388181418983	0.0	0.0	0.0	0.0
28	0.14192961545699767	0.0	0.0	0.0	0.0
29	0.14898688915375446	0.0	0.0	0.0	0.0
30	0.15584812747004578	0.0	0.0	0.0	0.0
31	0.1637875603788972	0.0	0.0	0.0	0.0
32	0.1703547456244903	0.0	0.0	0.0	0.0
33	0.17662787779938524	0.0	0.0	0.0	0.0
34	0.18544946992033123	0.0	0.0	0.0	0.0
35	0.19740762812872467	0.0	0.0	0.0	0.0
36	0.20887569788595445	0.0	0.0	0.0	0.0
37	0.21720720155573678	0.0	0.0	0.0	0.0
38	0.2240684398720281	0.0	0.0	0.0	0.0
39	0.23043958973715575	0.0	0.0	0.0	0.0
40	0.23837902264600716	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGATA	35	1.0194162E-7	46.000004	30
TCTACTA	35	1.0194162E-7	46.000004	13
ATATACG	35	1.0194162E-7	46.000004	10
AGCGATT	35	1.0194162E-7	46.000004	11
ACCACGA	35	1.0194162E-7	46.000004	18
AACGTTA	25	3.4168843E-5	46.0	44
AGCGTAT	20	6.311119E-4	46.0	23
TCGTTAA	20	6.311119E-4	46.0	35
CGTATTC	25	3.4168843E-5	46.0	20
CGTATAT	25	3.4168843E-5	46.0	34
CAACGTC	20	6.311119E-4	46.0	24
CGAAATA	25	3.4168843E-5	46.0	38
CCGATTC	20	6.311119E-4	46.0	39
ACGCATT	30	1.8611972E-6	46.0	44
GCGAAAC	30	1.8611972E-6	46.0	9
GCAACGT	45	3.092282E-10	46.0	14
GACGTAT	25	3.4168843E-5	46.0	42
CGCATAT	55	1.8189894E-12	46.0	22
CGCGCAA	20	6.311119E-4	46.0	45
CGATTGG	50	1.6370905E-11	46.0	23
>>END_MODULE