FastQCFastQC Report
Fri 17 Jun 2016
SRR1527319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996233
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT34520.34650528541014No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA32620.32743344177516703No Hit
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG27920.28025572330970766No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC23890.23980333917868613No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG23460.23548707982971856No Hit
ACGTTGGGGGTGGGGTCCCTTGGCACGGTACTGTCTGCAGCCCCTTGTCTCT21640.21721826118990237No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC18510.1857999082543943No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG18430.18499688325923755No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC16660.16722995524139433No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG15100.1515709678358376No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC13690.1374176522961998No Hit
ACGTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT13130.1317964773301025No Hit
ACGTTGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG12930.12978891484221058No Hit
ACGTTGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG12370.1241677398761133No Hit
ACGTTGGGGACTATCCATCTATCTATCTATCCATCATCTATCTACCTACCTA12140.12185904301503765No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT12090.12135715239306467No Hit
ACGTTGGGGTGGTTCACTTTTTATAGAAATTAATGTAGTGAAACACTAAAGA10910.10951253371450254No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC10660.10700308060463767No Hit
ACGTTGGGGGAGACGTGCCGTGGTCTCAGCAATGCACCTGTTTTATACATGA10610.10650118998266471No Hit
ACGTTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG10540.10579854311190255No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT10060.10098039314096201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA2100.046.00000424
CTAATAC551.8189894E-1246.00000423
TAATAGC501.6370905E-1146.014
TAATAGA950.046.035
CAAACGG253.4168253E-546.020
CGTATTC405.6097633E-946.020
GCGGTAA206.3110475E-446.09
CGCATGA206.3110475E-446.028
TAAACGT453.092282E-1046.038
CACGATT253.4168253E-546.021
CGATTCG206.3110475E-446.046
GGATACA900.046.08
CGACAAA600.046.015
TATGCGG206.3110475E-446.040
CGTTCGA405.6097633E-946.042
TCGATAC453.092282E-1046.025
CGTTCCA253.4168253E-546.013
TTATACC253.4168253E-546.013
ATATCGC206.3110475E-446.034
CCGTACG453.092282E-1046.015
Produced by FastQC (version 0.11.5)