FastQCFastQC Report
Fri 17 Jun 2016
SRR1527318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767849
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC97970.5541762899433152No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG75560.42741206969599776No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC61130.3457874513038161No Hit
TCTACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC46500.26303151456940044No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT44390.2510961060588319No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT42150.2384253406258114No Hit
TCTACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA40970.23175056240663086No Hit
TCTACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC36280.20522114728124408No Hit
TCTACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG35320.19979081923852093No Hit
TCTACCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAA35250.19939485781873906No Hit
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT33880.19164532717443628No Hit
TCTACCGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA33250.18808167439639925No Hit
TCTACCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG32580.1842917579499154No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC32200.18214225309967086No Hit
TCTACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA31830.18004931416653797No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGA30590.17303514044468732No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT30540.1727523108591288No Hit
TCTACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA29250.1654553075517196No Hit
TCTACCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA27130.15346333312403942No Hit
TCTACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA26450.14961685076044393No Hit
TCTACCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT26400.1493340211748854No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTG24710.13977438118300828No Hit
TCTACCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG24710.13977438118300828No Hit
TCTACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA23240.13145919136758855No Hit
TCTACCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT22330.1263116929104239No Hit
TCTACCGGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGT21920.12399249030884424No Hit
TCTACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC21820.12342683113772726No Hit
TCTACCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT20270.11465911398541391No Hit
TCTACCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACACTGGCT20070.11352779564317993No Hit
TCTACCGGGACCTCCATTACTTTGAAAGAAGTATGATTACCTTTAGCTGA19540.11052980203625988No Hit
TCTACCGGGAGCTCTGGGCGTGGCTCAGCACTGTCCCTCAGCACTGCTCC19510.11036010428492479No Hit
TCTACCGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTA18450.10436411707108469No Hit
TCTACCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC18060.10215804630372843No Hit
TCTACCGGGGTCCCCAGCCTGGTAGATCCCCCTTTCTCAGCATTTGGGAT17880.10113985979571785No Hit
TCTACCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA17870.10108329387860614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT650.044.00000441
GTCATAT207.8576326E-444.033
CGAACTA207.8576326E-444.028
AATTTCG207.8576326E-444.019
GCTATAT302.5284935E-644.015
ATAGACG254.4438162E-544.036
TAAAACG302.5284935E-644.027
TCGATAC207.8576326E-444.044
CGTTATC600.044.031
CGTTAAC254.4438162E-544.044
GTTACGG302.5284935E-644.033
ACGATTA1000.044.010
TACGCTT254.4438162E-544.024
ACGATAC207.8576326E-444.025
CCGTACG254.4438162E-544.038
TACGCAC207.8576326E-444.038
CTTATCA207.8576326E-444.037
CGCTATA302.5284935E-644.036
CTCGATA207.8576326E-444.031
CGTAATA302.5284935E-644.044
Produced by FastQC (version 0.11.5)