FastQCFastQC Report
Fri 17 Jun 2016
SRR1527317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897295
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC95600.5038752539800083No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT71850.3786970397328829No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA60810.32050893508916645No Hit
TCTACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC45330.23891909270830314No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT43340.22843047601980715No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT39640.2089290279055181No Hit
TCTACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG37390.19707003918736937No Hit
TCTACCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG34340.18099452114721223No Hit
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG34290.18073098806458668No Hit
TCTACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT33620.17719964475740463No Hit
TCTACCGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG32780.17277228896929578No Hit
TCTACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC32230.16987342506041495No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC30580.16117683333377256No Hit
TCTACCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG30440.16043894070242107No Hit
TCTACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA29610.1560642915308373No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT29040.1530600143889063No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG28640.1509517497279021No Hit
TCTACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG28380.14958137769824933No Hit
TCTACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG27420.1445215425118392No Hit
TCTACCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG25510.13445457875554406No Hit
TCTACCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG25130.13245172732759006No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCT23850.12570528041237655No Hit
TCTACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC22360.11785199455013584No Hit
TCTACCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT21620.11395170492727806No Hit
TCTACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT20210.10652007199723817No Hit
TCTACCGGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGTGT20050.10567676613283648No Hit
TCTACCGGGACCTCCATTACTTTGAAAGAAGTATGATTACCTTTAGCTGATA19700.10383203455445779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA206.312497E-446.00000425
CGATTTA206.312497E-446.00000418
TATGCGG206.312497E-446.00000415
TCGATAG405.6152203E-946.00000446
ATACTCG351.0200347E-746.00000421
CTAACGC206.312497E-446.00000419
CCGTTAT351.0200347E-746.00000430
TAGTACG206.312497E-446.00000436
TCGTTTA551.8189894E-1246.043
AATTTCG501.6370905E-1146.019
CCGATTA253.4180026E-546.044
GCGAATA253.4180026E-546.030
CCGATAA253.4180026E-546.040
CAAATCG600.046.034
TCGTAAA253.4180026E-546.027
CGTAAAT453.110472E-1046.039
CGTCATA253.4180026E-546.038
AATCGCC253.4180026E-546.024
ACCGGGG984900.044.87444
CGGGATA19750.044.8354456
Produced by FastQC (version 0.11.5)