##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527317_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1897295 Sequences flagged as poor quality 0 Sequence length 52 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.591978580031046 34.0 31.0 34.0 31.0 34.0 2 32.84227597711479 34.0 31.0 34.0 31.0 34.0 3 32.97458434244543 34.0 31.0 34.0 31.0 34.0 4 36.410291493942694 37.0 37.0 37.0 35.0 37.0 5 36.38759075420533 37.0 37.0 37.0 35.0 37.0 6 36.40824173362603 37.0 37.0 37.0 35.0 37.0 7 36.533598096237014 37.0 37.0 37.0 35.0 37.0 8 36.61877884040173 37.0 37.0 37.0 35.0 37.0 9 38.67577103191649 39.0 39.0 39.0 38.0 39.0 10 38.12948381775106 39.0 39.0 39.0 37.0 39.0 11 37.885293536324085 39.0 38.0 39.0 35.0 39.0 12 37.37505712079566 39.0 37.0 39.0 35.0 39.0 13 37.29137904226807 39.0 37.0 39.0 35.0 39.0 14 38.12131060272651 40.0 38.0 40.0 35.0 40.0 15 37.99835449943209 40.0 38.0 40.0 34.0 40.0 16 32.900011331922556 40.0 34.0 40.0 3.0 40.0 17 34.51426899875876 40.0 34.0 40.0 17.0 40.0 18 36.54580125916107 40.0 34.0 40.0 30.0 40.0 19 37.450624705172366 40.0 36.0 40.0 33.0 40.0 20 37.71494311638411 40.0 37.0 40.0 33.0 40.0 21 37.9196029083511 40.0 37.0 40.0 34.0 40.0 22 37.96276488368967 40.0 37.0 40.0 34.0 40.0 23 37.93678263000746 40.0 37.0 40.0 34.0 40.0 24 37.899260789703234 40.0 37.0 40.0 34.0 40.0 25 37.82058298788539 40.0 37.0 40.0 34.0 40.0 26 37.7240834978219 40.0 37.0 40.0 34.0 40.0 27 37.55472238107411 40.0 36.0 40.0 33.0 40.0 28 37.431724112486464 40.0 36.0 40.0 33.0 40.0 29 37.19250933565945 40.0 35.0 40.0 33.0 40.0 30 37.158591046727054 39.0 35.0 40.0 33.0 40.0 31 37.12289232828843 39.0 35.0 40.0 33.0 40.0 32 36.95911916702463 39.0 35.0 40.0 32.0 40.0 33 36.55472238107411 39.0 35.0 40.0 31.0 40.0 34 36.366530244374225 38.0 35.0 40.0 31.0 40.0 35 36.316079471036396 38.0 35.0 40.0 31.0 40.0 36 36.24887958909922 38.0 35.0 40.0 31.0 40.0 37 35.883044544996956 38.0 35.0 40.0 30.0 40.0 38 35.84617890206847 38.0 35.0 40.0 30.0 40.0 39 35.44013029075605 37.0 34.0 40.0 29.0 40.0 40 35.34419423442322 37.0 34.0 40.0 29.0 40.0 41 35.27725893970099 37.0 34.0 40.0 29.0 40.0 42 35.15925146063211 36.0 34.0 40.0 29.0 40.0 43 35.0546936559681 36.0 34.0 40.0 29.0 40.0 44 35.28924020776948 36.0 34.0 40.0 30.0 40.0 45 35.3308088620905 36.0 35.0 40.0 30.0 40.0 46 35.211501637858106 36.0 34.0 40.0 30.0 40.0 47 34.90960920679178 35.0 34.0 39.0 29.0 40.0 48 34.8398124698584 35.0 34.0 39.0 29.0 40.0 49 34.7574436236853 35.0 34.0 39.0 29.0 40.0 50 34.234602420814895 35.0 34.0 39.0 27.0 40.0 51 34.28327276464651 35.0 34.0 39.0 28.0 40.0 52 33.902907033434445 35.0 33.0 38.0 27.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 2.0 15 17.0 16 40.0 17 108.0 18 246.0 19 445.0 20 802.0 21 1495.0 22 2456.0 23 3863.0 24 5521.0 25 8113.0 26 11000.0 27 15113.0 28 20355.0 29 26412.0 30 33840.0 31 43557.0 32 59519.0 33 90328.0 34 138069.0 35 195180.0 36 264681.0 37 381119.0 38 416649.0 39 178362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.18120271228248 0.3615673893622236 0.12528362748017574 0.3319462708751143 8 98.70747564295483 0.9801849475173865 0.16223096566427467 0.1501084438635004 9 94.88598241180206 2.657889258128019 1.040323196972532 1.4158051330973833 10 53.60062615460431 31.330394061018453 5.76125483912623 9.307724945251001 11 36.48736754168434 20.33189356425859 21.552051736814782 21.628687157242286 12 30.29349679412005 15.065711974152677 28.375555725387986 26.26523550633929 13 25.648304559912926 16.108828621801038 30.898199805512583 27.34466701277345 14 25.45371173170224 16.630782245249158 31.043037587723575 26.872468435325032 15 27.92812925770637 17.47187443175679 28.7822399784957 25.817756332041142 16 29.593711046516226 27.180485902297747 22.598805141003375 20.626997910182652 17 32.27505474899792 18.663254791690274 23.91889505849117 25.142795400820646 18 31.356431129581853 19.68244263543624 23.362998374000878 25.59812786098103 19 30.690324909937566 20.818112101702688 22.35129486980148 26.140268118558264 20 29.014359917672266 21.60138512988228 24.676605377656085 24.70764957478937 21 30.239999578347067 19.35418582771788 26.26418137400879 24.141633219926266 22 29.937569012726012 18.52310789835002 25.75651124363897 25.782811845284996 23 30.15134704935184 20.00932907112494 24.519961313343472 25.31936256617974 24 30.125784340337162 20.272598620667846 22.30670507222124 27.29491196677375 25 27.294490313841546 19.211509016784422 23.54984332958238 29.944157339791648 26 26.518174559043267 19.60749382673754 23.713708200358933 30.16062341386026 27 27.10010831209696 19.183732629875692 23.654097017069038 30.06206204095831 28 28.817447998334472 21.327363430568255 21.631796847617267 28.223391723480006 29 30.365968391842067 20.193696815729762 22.374274954606427 27.066059837821744 30 29.6820473358123 19.54419318029089 22.579461812738664 28.194297671158147 31 32.099330889503214 19.265849538421804 21.875143296113677 26.759676275961304 32 33.94801546412129 18.68312518612024 23.105579258892263 24.263280090866207 33 32.71974047262023 20.006324793983012 23.963274029605305 23.31066070379145 34 28.278839084064415 21.520111527200566 27.16878503342917 23.03226435530584 35 28.39379221470567 25.721303223800202 24.86466258541766 21.020241976076466 36 30.656381848895403 24.374965411282908 21.89490827731059 23.0737444625111 37 30.317267478172873 25.21589947794096 21.206349039026616 23.26048400485955 38 31.043933600204504 24.07901775949444 21.65546211843704 23.221586521864023 39 30.862886372440762 22.208987005183694 20.678650394377257 26.24947622799828 40 28.884965174103133 22.386555596256777 21.964006651575005 26.764472578065085 41 25.80732042196917 23.3109769434906 22.570027328380668 28.311675306159557 42 26.94430755364875 20.600064829138326 23.568132525516592 28.887495091696337 43 25.881478631419995 19.67158507243207 25.600236125642034 28.84670017050591 44 26.98842299168026 20.577980756814306 24.92053159893427 27.513064652571163 45 27.167203834933417 20.378907866198983 24.172413883976926 28.281474414890674 46 27.673767126356207 21.25109695645643 24.122606131360698 26.952529785826663 47 27.265554381369267 21.559061716812618 25.238932269362437 25.936451632455682 48 29.022634856466706 20.75228153766283 24.692206536147516 25.532877069722947 49 27.89618904809215 21.867079183785336 23.967174319228164 26.26955744889435 50 25.770952856566847 22.470886182696944 25.075014691969354 26.68314626876685 51 26.333332454889725 21.596483414545446 24.719034203958795 27.351149926606038 52 26.86482597592889 22.184531135116046 24.46071907636925 26.489923812585815 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 29.5 6 59.0 7 55.0 8 51.0 9 190.0 10 329.0 11 627.5 12 926.0 13 1607.5 14 2989.5 15 3690.0 16 3375.0 17 3060.0 18 3855.0 19 4650.0 20 4521.0 21 4392.0 22 4471.5 23 4551.0 24 4896.0 25 5241.0 26 7669.0 27 10097.0 28 9696.0 29 9295.0 30 10354.5 31 11414.0 32 12123.0 33 12832.0 34 15290.5 35 17749.0 36 21439.0 37 25129.0 38 25652.5 39 27540.0 40 28904.0 41 30970.5 42 33037.0 43 38972.5 44 44908.0 45 45692.0 46 46476.0 47 53726.0 48 60976.0 49 64726.5 50 68477.0 51 74431.0 52 80385.0 53 95290.0 54 110195.0 55 123870.0 56 137545.0 57 147781.5 58 158018.0 59 171783.0 60 185548.0 61 183218.0 62 180888.0 63 176724.5 64 150674.0 65 128787.0 66 115678.5 67 102570.0 68 84876.0 69 67182.0 70 62194.0 71 57206.0 72 48359.5 73 39513.0 74 34560.5 75 29608.0 76 22000.0 77 14392.0 78 10024.5 79 5657.0 80 3742.0 81 1827.0 82 1162.0 83 497.0 84 325.0 85 153.0 86 89.0 87 25.0 88 19.0 89 11.0 90 9.0 91 6.0 92 3.0 93 4.0 94 5.0 95 2.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1897295.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.275076759814025 #Duplication Level Percentage of deduplicated Percentage of total 1 71.1239421543585 6.596800229422477 2 10.01543126262746 1.857877874870216 3 4.124177957333116 1.14756201376193 4 2.147252020246889 0.7966370924182256 5 1.2260382505479084 0.5685799442149976 6 0.7604752793686222 0.42320799540509924 7 0.49800038706585015 0.32332942715169993 8 0.3612705292972276 0.2680649512232342 9 0.2690548666923605 0.22459540870558561 >10 3.873144271264822 9.80864996630048 >50 2.8914106031560727 19.55393643598921 >100 2.5464201439771625 41.87872980007771 >500 0.11977027262060273 7.54485716194879 >1k 0.04165922525934008 7.629078654806366 >5k 0.0019527761840315663 1.378093043703983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 9560 0.5038752539800083 No Hit TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 7185 0.3786970397328829 No Hit TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 6081 0.32050893508916645 No Hit TCTACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 4533 0.23891909270830314 No Hit TCTACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT 4334 0.22843047601980715 No Hit TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 3964 0.2089290279055181 No Hit TCTACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG 3739 0.19707003918736937 No Hit TCTACCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG 3434 0.18099452114721223 No Hit TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 3429 0.18073098806458668 No Hit TCTACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 3362 0.17719964475740463 No Hit TCTACCGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG 3278 0.17277228896929578 No Hit TCTACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC 3223 0.16987342506041495 No Hit TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 3058 0.16117683333377256 No Hit TCTACCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG 3044 0.16043894070242107 No Hit TCTACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA 2961 0.1560642915308373 No Hit TCTACCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT 2904 0.1530600143889063 No Hit TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG 2864 0.1509517497279021 No Hit TCTACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG 2838 0.14958137769824933 No Hit TCTACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG 2742 0.1445215425118392 No Hit TCTACCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG 2551 0.13445457875554406 No Hit TCTACCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG 2513 0.13245172732759006 No Hit TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCT 2385 0.12570528041237655 No Hit TCTACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC 2236 0.11785199455013584 No Hit TCTACCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 2162 0.11395170492727806 No Hit TCTACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT 2021 0.10652007199723817 No Hit TCTACCGGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGTGT 2005 0.10567676613283648 No Hit TCTACCGGGACCTCCATTACTTTGAAAGAAGTATGATTACCTTTAGCTGATA 1970 0.10383203455445779 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.162396991506329E-4 0.0 0.0 0.0 0.0 8 5.797727817761603E-4 0.0 0.0 0.0 0.0 9 8.433058644016876E-4 0.0 0.0 0.0 0.0 10 0.0037948763898075946 0.0 0.0 0.0 0.0 11 0.007168099847414345 0.0 0.0 0.0 0.0 12 0.01048861668849599 0.0 0.0 0.0 0.0 13 0.014388906311353795 0.0 0.0 0.0 0.0 14 0.024561283300699153 0.0 0.0 0.0 0.0 15 0.031518556682013074 0.0 0.0 0.0 0.0 16 0.045327690211590714 0.0 0.0 0.0 0.0 17 0.05154707096155316 0.0 0.0 0.0 0.0 18 0.05718667892973944 0.0 0.0 0.0 0.0 19 0.06778070885128565 0.0 0.0 0.0 0.0 20 0.0761083542622523 0.0 0.0 0.0 0.0 21 0.08301292102704112 0.0 0.0 0.0 0.0 22 0.09107703335538227 0.0 0.0 0.0 0.0 23 0.09724370748881961 0.0 0.0 0.0 0.0 24 0.10514969996758543 0.0 0.0 0.0 0.0 25 0.11100013440187213 0.0 0.0 0.0 0.0 26 0.11895883349716306 0.0 0.0 0.0 0.0 27 0.12860414432125736 0.0 0.0 0.0 0.0 28 0.14373094326396263 0.0 0.0 0.0 0.0 29 0.1491597247660485 0.0 0.0 0.0 0.0 30 0.15732925032743986 0.0 0.0 0.0 0.0 31 0.1625999119799504 0.0 0.0 0.0 0.0 32 0.16971530521083963 0.0 0.0 0.0 0.0 33 0.1760400991938523 0.0 0.0 0.0 0.0 34 0.18510563723617043 0.0 0.0 0.0 0.0 35 0.2066626433949386 0.0 0.0 0.0 0.0 36 0.21999741737579026 0.0 0.0 0.0 0.0 37 0.22774529000498078 0.0 0.0 0.0 0.0 38 0.23459715015324448 0.0 0.0 0.0 0.0 39 0.2407111176701567 0.0 0.0 0.0 0.0 40 0.24666696533749363 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAA 20 6.312497E-4 46.000004 25 CGATTTA 20 6.312497E-4 46.000004 18 TATGCGG 20 6.312497E-4 46.000004 15 TCGATAG 40 5.6152203E-9 46.000004 46 ATACTCG 35 1.0200347E-7 46.000004 21 CTAACGC 20 6.312497E-4 46.000004 19 CCGTTAT 35 1.0200347E-7 46.000004 30 TAGTACG 20 6.312497E-4 46.000004 36 TCGTTTA 55 1.8189894E-12 46.0 43 AATTTCG 50 1.6370905E-11 46.0 19 CCGATTA 25 3.4180026E-5 46.0 44 GCGAATA 25 3.4180026E-5 46.0 30 CCGATAA 25 3.4180026E-5 46.0 40 CAAATCG 60 0.0 46.0 34 TCGTAAA 25 3.4180026E-5 46.0 27 CGTAAAT 45 3.110472E-10 46.0 39 CGTCATA 25 3.4180026E-5 46.0 38 AATCGCC 25 3.4180026E-5 46.0 24 ACCGGGG 98490 0.0 44.8744 4 CGGGATA 1975 0.0 44.835445 6 >>END_MODULE