FastQCFastQC Report
Fri 17 Jun 2016
SRR1527316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838576
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC90900.49440436511735164No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT69980.38062065424545954No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA57570.31312276457432275No Hit
TCTACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC44340.24116490153249037No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT40030.21772284637676115No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT39390.2142418915508524No Hit
TCTACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT35420.19264909364638722No Hit
TCTACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG34850.18954886825456224No Hit
TCTACCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG33650.18302207795598333No Hit
TCTACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC33010.17954112313007456No Hit
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG32450.1764952876574044No Hit
TCTACCGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG31040.16882630905657423No Hit
TCTACCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG30730.16714022156277467No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC29300.15936246312363483No Hit
TCTACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA28390.1544129804805458No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG27920.1518566542802691No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT27750.15093202565463706No Hit
TCTACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG27220.14804935993943139No Hit
TCTACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG27090.14734229099041868No Hit
TCTACCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG24720.13445188015072534No Hit
TCTACCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG24440.13292896241439026No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCT23170.1260214426817276No Hit
TCTACCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT21410.11644881691047855No Hit
TCTACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC21270.115687358042311No Hit
TCTACCGGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGTGT20130.10948690725866106No Hit
TCTACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT18870.1026337774451532No Hit
TCTACCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACACTGGCTGC18710.10176353873867602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAA206.3124485E-446.019
CGTTATA206.3124485E-446.036
CGTTAGA206.3124485E-446.023
TATTCGC206.3124485E-446.022
CACGTTA301.8620158E-646.043
CGTCATA206.3124485E-446.038
TATAACG253.4179604E-546.045
CGATATC301.8620158E-646.033
AATACGC301.8620158E-646.036
ACCGGGG967200.044.7111244
TCTACCG1877950.044.684631
TACCGGG1763750.044.6594473
CTACCGG1858200.044.6508452
CGGGATA16850.044.6350146
CCGGGAT141150.044.5334745
CGGGATT50950.044.239456
CCGGGGG377200.044.225615
ACCGGGA558700.044.205124
ACCGGGC155800.044.1399234
CGGGGGG177400.043.9904176
Produced by FastQC (version 0.11.5)