##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527316_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1838576
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57739631105812	34.0	31.0	34.0	31.0	34.0
2	32.84225835646718	34.0	31.0	34.0	31.0	34.0
3	32.942949326000125	34.0	31.0	34.0	31.0	34.0
4	36.41026479188241	37.0	37.0	37.0	35.0	37.0
5	36.40113870734743	37.0	37.0	37.0	35.0	37.0
6	36.41154349888174	37.0	37.0	37.0	35.0	37.0
7	36.534515842695654	37.0	37.0	37.0	35.0	37.0
8	36.64492955417671	37.0	37.0	37.0	35.0	37.0
9	38.620255023452934	39.0	39.0	39.0	38.0	39.0
10	38.12333131728033	39.0	39.0	39.0	37.0	39.0
11	37.81084763425608	39.0	38.0	39.0	35.0	39.0
12	37.33498424867941	39.0	37.0	39.0	35.0	39.0
13	37.195328341063956	39.0	37.0	39.0	34.0	39.0
14	38.05123747944061	40.0	38.0	40.0	34.0	40.0
15	38.11670553732889	40.0	38.0	40.0	34.0	40.0
16	38.16590230700281	40.0	38.0	40.0	35.0	40.0
17	38.12856689089817	40.0	38.0	40.0	34.0	40.0
18	38.10090907310875	40.0	38.0	40.0	34.0	40.0
19	38.03428359774086	40.0	38.0	40.0	34.0	40.0
20	37.9705908268138	40.0	38.0	40.0	34.0	40.0
21	33.63500121833419	40.0	34.0	40.0	10.0	40.0
22	34.313611729947525	39.0	34.0	40.0	16.0	40.0
23	36.40207802125123	39.0	34.0	40.0	30.0	40.0
24	37.23334417505722	39.0	36.0	40.0	32.0	40.0
25	37.514379606826154	39.0	36.0	40.0	33.0	40.0
26	37.601656934497136	40.0	36.0	40.0	33.0	40.0
27	37.51646491632655	40.0	36.0	40.0	33.0	40.0
28	37.40085533586863	40.0	36.0	40.0	33.0	40.0
29	37.36932821923053	40.0	35.0	40.0	33.0	40.0
30	37.23230695929894	39.0	35.0	40.0	33.0	40.0
31	37.08431525267381	39.0	35.0	40.0	33.0	40.0
32	36.95380011487151	39.0	35.0	40.0	32.0	40.0
33	36.563003650651375	39.0	35.0	40.0	31.0	40.0
34	36.36569007753827	38.0	35.0	40.0	31.0	40.0
35	36.31752399683233	38.0	35.0	40.0	31.0	40.0
36	36.185591457736855	38.0	35.0	40.0	31.0	40.0
37	36.04365770030719	38.0	35.0	40.0	31.0	40.0
38	35.93875314373733	38.0	35.0	40.0	31.0	40.0
39	35.737389697243955	37.0	35.0	40.0	30.0	40.0
40	30.87083753948708	35.0	30.0	40.0	8.0	40.0
41	31.659233559015238	35.0	30.0	39.0	12.0	40.0
42	33.37641033060368	35.0	30.0	39.0	23.0	40.0
43	34.25362073691814	35.0	33.0	39.0	27.0	40.0
44	34.9684027203662	36.0	34.0	39.0	29.0	40.0
45	35.202154275917884	36.0	34.0	40.0	30.0	40.0
46	35.18390591414225	35.0	34.0	40.0	30.0	40.0
47	35.12176488760867	35.0	34.0	39.0	30.0	40.0
48	34.96424841834115	35.0	34.0	39.0	30.0	40.0
49	34.82118389449226	35.0	34.0	39.0	30.0	40.0
50	34.67602916605025	35.0	34.0	39.0	29.0	40.0
51	34.47894511839598	35.0	34.0	39.0	29.0	40.0
52	34.047726066259976	35.0	34.0	38.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	6.0
15	18.0
16	55.0
17	129.0
18	229.0
19	526.0
20	935.0
21	1542.0
22	2633.0
23	4046.0
24	5933.0
25	8473.0
26	11569.0
27	15761.0
28	21013.0
29	27464.0
30	36270.0
31	48571.0
32	71483.0
33	90152.0
34	155758.0
35	209992.0
36	250811.0
37	290694.0
38	403396.0
39	181114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.18801289693762	0.3553837317576211	0.12618461243919207	0.3304187588655568
8	98.72314225792135	0.9789641548676802	0.14843008937351515	0.1494634978374568
9	94.83415425851311	2.6571107204706252	1.0982956374933643	1.4104393835229003
10	53.64407019345406	31.300582624813988	5.762013645342918	9.293333536389031
11	36.63650564349801	20.276616250837602	21.483854896398082	21.6030232092663
12	30.366598933087346	15.055782301085188	28.313053145477806	26.26456562034966
13	25.750907223851506	16.08440445214122	30.843979253509236	27.32070907049804
14	25.53568631375586	16.643641600891122	31.00421195533935	26.816460130013663
15	27.91094847316619	17.355714422466082	28.787278850588716	25.946058253779015
16	31.213069244893877	19.085857750781038	25.69439609784964	24.006676906475448
17	32.40540505260593	18.640132363307256	23.895612691561297	25.05884989252552
18	31.444443960978496	19.656734342230074	23.297377970777383	25.601443726014043
19	30.75630270383166	20.85053867775931	22.293503232936796	26.099655385472236
20	29.005817545752798	21.629783049490474	24.580599333397153	24.783800071359575
21	34.668297639042386	18.8368063109711	24.834926595365108	21.659969454621404
22	30.129295715814848	18.501438069462452	25.699508750250192	25.669757464472504
23	30.197772624030772	20.025878723533864	24.428307559763642	25.348041092671718
24	30.25085718512588	20.280369155259287	22.215725648545394	27.253048011069435
25	27.303249906449338	19.17946280164649	23.532614371121998	29.984672920782167
26	26.459988599872947	19.573789715518966	23.743484087685253	30.222737596922837
27	27.10189842573818	19.16129656864878	23.62485967400858	30.11194533160446
28	28.773898930476633	21.289356545500432	21.63489570189103	28.30184882213191
29	30.278976773328925	20.210423719226185	22.380200764069585	27.130398743375306
30	29.68389666785599	19.478716136836336	22.61162987007336	28.225757325234312
31	32.20503259043956	19.27187127429054	21.783217011426235	26.739879123843668
32	33.996473357641996	18.73634813029214	23.056920138193906	24.210258373871955
33	32.69454186283298	20.028217490057525	23.93129247852686	23.34594816858264
34	28.251810096509473	21.569627798905238	27.149163265483722	23.029398839101567
35	28.393767785503567	25.725670301363664	24.839984857846506	21.040577055286263
36	30.788719095647938	24.354010930198154	21.846472487403297	23.010797486750615
37	30.27973823219709	25.151530314765342	21.20880507523214	23.359926377805433
38	31.035649328610837	24.080647196526005	21.587141352873093	23.29656212199006
39	30.71322588786104	22.170636405566047	20.63112974388875	26.485007962684165
40	29.120797834846098	26.887330194672398	20.248768612230332	23.74310335825117
41	25.841466439244286	23.3617756350567	22.548972683207	28.247785242492014
42	26.96760971534492	20.550795833297073	23.524782222763704	28.956812228594302
43	25.940020972752826	19.656190443038525	25.50811062474437	28.895677959464283
44	27.07084178190077	20.572116681605763	24.878438530688967	27.47860300580449
45	27.231944722437362	20.345201938891837	24.135635404791532	28.287217933879262
46	27.705191409003486	21.221967435667604	24.090165432378104	26.982675722950805
47	27.007477526085406	21.595571790342092	25.241328071289953	26.15562261228255
48	29.045522186735823	20.7743384010234	24.688291373323704	25.491848038917077
49	27.995470407532785	21.852455378510324	23.8325203853417	26.319553828615188
50	25.753300380294313	22.43486263282018	25.123410726562295	26.688426260323205
51	26.360346267981306	21.645991245398612	24.694654993864816	27.299007492755262
52	26.946832766227775	22.10944774651687	24.508151961082923	26.435567526172427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	18.5
6	37.0
7	47.5
8	58.0
9	159.5
10	261.0
11	540.5
12	820.0
13	1454.0
14	2716.0
15	3344.0
16	3088.5
17	2833.0
18	3606.0
19	4379.0
20	4277.5
21	4176.0
22	4322.5
23	4469.0
24	4808.0
25	5147.0
26	7402.5
27	9658.0
28	9268.0
29	8878.0
30	9804.5
31	10731.0
32	11450.5
33	12170.0
34	14580.0
35	16990.0
36	20391.0
37	23792.0
38	24278.5
39	26369.5
40	27974.0
41	29640.5
42	31307.0
43	37366.0
44	43425.0
45	44099.5
46	44774.0
47	51787.5
48	58801.0
49	62029.5
50	65258.0
51	71833.5
52	78409.0
53	92143.5
54	105878.0
55	118171.5
56	130465.0
57	141952.5
58	153440.0
59	166963.0
60	180486.0
61	176958.5
62	173431.0
63	171289.5
64	147900.5
65	126653.0
66	113958.0
67	101263.0
68	83717.5
69	66172.0
70	61309.5
71	56447.0
72	47571.5
73	38696.0
74	34017.0
75	29338.0
76	21856.5
77	14375.0
78	10034.5
79	5694.0
80	3775.0
81	1856.0
82	1166.0
83	476.0
84	321.0
85	166.0
86	97.0
87	28.0
88	19.0
89	9.0
90	8.0
91	4.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1838576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.582646188793193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28710262935742	6.927017285101142
2	9.352625298283202	1.7924579833960865
3	3.8582773886699027	1.1091752134153376
4	2.080970073454167	0.7976479977351304
5	1.1262316769131542	0.5396139843235
6	0.709192059846385	0.4077561953645613
7	0.4993301695436077	0.33494330422885576
8	0.34190360202156245	0.26210729990772724
9	0.24833028076581262	0.2141695096688212
>10	4.014735961866518	10.573565663893891
>50	2.9123308913352854	20.304775518443993
>100	2.417313422429333	40.8130718915553
>500	0.1089826257803556	6.998160528787645
>1k	0.04070435420712076	7.5512213737274765
>5k	0.0019695655261510047	1.3743162504505344
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9090	0.49440436511735164	No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6998	0.38062065424545954	No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5757	0.31312276457432275	No Hit
TCTACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4434	0.24116490153249037	No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	4003	0.21772284637676115	No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3939	0.2142418915508524	No Hit
TCTACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3542	0.19264909364638722	No Hit
TCTACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	3485	0.18954886825456224	No Hit
TCTACCGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG	3365	0.18302207795598333	No Hit
TCTACCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3301	0.17954112313007456	No Hit
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3245	0.1764952876574044	No Hit
TCTACCGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	3104	0.16882630905657423	No Hit
TCTACCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3073	0.16714022156277467	No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2930	0.15936246312363483	No Hit
TCTACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2839	0.1544129804805458	No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG	2792	0.1518566542802691	No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	2775	0.15093202565463706	No Hit
TCTACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2722	0.14804935993943139	No Hit
TCTACCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2709	0.14734229099041868	No Hit
TCTACCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2472	0.13445188015072534	No Hit
TCTACCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2444	0.13292896241439026	No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCT	2317	0.1260214426817276	No Hit
TCTACCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2141	0.11644881691047855	No Hit
TCTACCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2127	0.115687358042311	No Hit
TCTACCGGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGTGT	2013	0.10948690725866106	No Hit
TCTACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1887	0.1026337774451532	No Hit
TCTACCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACACTGGCTGC	1871	0.10176353873867602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.631697574644725E-4	0.0	0.0	0.0	0.0
8	3.26339514928945E-4	0.0	0.0	0.0	0.0
9	6.5267902985789E-4	0.0	0.0	0.0	0.0
10	0.00456875320900523	0.0	0.0	0.0	0.0
11	0.007342639085901262	0.0	0.0	0.0	0.0
12	0.011313103184203427	0.0	0.0	0.0	0.0
13	0.016806485018840668	0.0	0.0	0.0	0.0
14	0.02714056965825726	0.0	0.0	0.0	0.0
15	0.03508149785486159	0.0	0.0	0.0	0.0
16	0.04710166998807773	0.0	0.0	0.0	0.0
17	0.05395479980158557	0.0	0.0	0.0	0.0
18	0.058360383253126334	0.0	0.0	0.0	0.0
19	0.06717155015620785	0.0	0.0	0.0	0.0
20	0.07522125819112183	0.0	0.0	0.0	0.0
21	0.0816936585705459	0.0	0.0	0.0	0.0
22	0.08876434806067304	0.0	0.0	0.0	0.0
23	0.09594381738910983	0.0	0.0	0.0	0.0
24	0.10431986493895276	0.0	0.0	0.0	0.0
25	0.11013958628851894	0.0	0.0	0.0	0.0
26	0.1179173447276588	0.0	0.0	0.0	0.0
27	0.12683729146904996	0.0	0.0	0.0	0.0
28	0.14532986398169018	0.0	0.0	0.0	0.0
29	0.15049690630139848	0.0	0.0	0.0	0.0
30	0.15702369659997736	0.0	0.0	0.0	0.0
31	0.16246268851545978	0.0	0.0	0.0	0.0
32	0.17002288727798034	0.0	0.0	0.0	0.0
33	0.17595138846585617	0.0	0.0	0.0	0.0
34	0.1856871839945697	0.0	0.0	0.0	0.0
35	0.20532194480946123	0.0	0.0	0.0	0.0
36	0.21598236896380676	0.0	0.0	0.0	0.0
37	0.22185648023252777	0.0	0.0	0.0	0.0
38	0.22870961004603563	0.0	0.0	0.0	0.0
39	0.23578029953616278	0.0	0.0	0.0	0.0
40	0.24268781926882543	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCAA	20	6.3124485E-4	46.0	19
CGTTATA	20	6.3124485E-4	46.0	36
CGTTAGA	20	6.3124485E-4	46.0	23
TATTCGC	20	6.3124485E-4	46.0	22
CACGTTA	30	1.8620158E-6	46.0	43
CGTCATA	20	6.3124485E-4	46.0	38
TATAACG	25	3.4179604E-5	46.0	45
CGATATC	30	1.8620158E-6	46.0	33
AATACGC	30	1.8620158E-6	46.0	36
ACCGGGG	96720	0.0	44.711124	4
TCTACCG	187795	0.0	44.68463	1
TACCGGG	176375	0.0	44.659447	3
CTACCGG	185820	0.0	44.650845	2
CGGGATA	1685	0.0	44.635014	6
CCGGGAT	14115	0.0	44.533474	5
CGGGATT	5095	0.0	44.23945	6
CCGGGGG	37720	0.0	44.22561	5
ACCGGGA	55870	0.0	44.20512	4
ACCGGGC	15580	0.0	44.139923	4
CGGGGGG	17740	0.0	43.990417	6
>>END_MODULE