FastQCFastQC Report
Fri 17 Jun 2016
SRR1527314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119849
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44443.7079992323673956No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG18811.5694749226109521No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15851.3224974759906214No Hit
GTAATGGGGATTTTAAGATTTTGGTAAAAGGATACTATAAAAACAATGTTTG7540.6291249822693556No Hit
GTAATGGGGGGAGGCCATCAACTACATGGCGGCGGATGGAGACTTCAAGATT6390.5331709067242948No Hit
GTAATGGGGGACTGTACTGGCCAGTGTCCTTTACTTTCCCTCCTGACAGATA6030.5031331091623626No Hit
GTAATGGGGATCCCTAGGAAGGATGAAATTTGAGAGGTGATCATGTCTTTTT5900.4922861267094427No Hit
GTAATGGGGGTGGCCAATGCATCCCGAGCAGCGGGCGGCTTCCCATGACTCC5710.4764328446628674No Hit
GTAATGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGC5390.4497325801633722No Hit
GTAATGGGGGTTAGGAGGAAGACGGAAGCTCCAGCCGGGCTTTGGGACTGGC5230.4363824479136247No Hit
GTAATGGGGATGGCATCAGGCTTTGGCAGATAGTGTCAGAGCTGTGTGTGTG5030.4196947826014401No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.4105165666797386No Hit
GTAATGGAGAGAAAAAATTTTTAGGTTTATTATTTTTTTATTTATATTTTTA4580.382147535649025No Hit
GTAATGGGGCTTCACCTACCCTGAAGCTGACTCCTTCCCAAGCTGTGCCGCT4510.37630685278976045No Hit
GTAATGGGGACATTTACTTATTTTGTGTGTGCAGATGCTCTAGCAGACATGT4450.3713005531961051No Hit
GTAATGGGGGCTCACGTCGCTGCCCGCTGATCGACGATGCGGTAATCCGTTT4380.3654598703368405No Hit
GTAATGGGGATCTTGACTTTTGATATTTTTTGGGGGGGTGGGGGTGGGCATC4350.3629567205400129No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4310.35961918747757593No Hit
GTAATGGGGTCTGTAGCACCTCCTGGCAGCACGCAGTTAAACCAGATCTACC3980.33208453971247154No Hit
GTAATGGGGATCAAGACTATCTACAAAAATTTATTATTTACAGAAAAAAGCA3510.29286852622883797No Hit
GTAATGGGGGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCC3490.2911997596976195No Hit
GTAATGGGGACGCAGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAA3440.2870278433695734No Hit
GTAATGGGGGGGCTGTTTGAGAAATAATGTGAGAGCAAATGTTAAAAGAAAG3240.27034017805738886No Hit
GTAATGGGGATTTGTTTCTGGTTTAGCCTCAAGGGCTGGAAGCTGGGCCTGT3230.26950579479177966No Hit
GTAATGGGGACCGTCGTTCCCCAGCTGATGAGTTCTGAATTCGGCAGTTGAA3020.25198374621398595No Hit
GTAATGGGGAGAAAGAGCAGCACAGCTGGCCATCAGAGTCACCAATCCCAAC3010.25114936294837675No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAA2940.24530868008911214No Hit
GTAATGGGGAAGTTTTTAGGTATTTAATTTGTAAAACAGTTTGCTTTGTACT2800.233627314370583No Hit
GTAATGGGGGTATCCATGAGAATGTGGGTTTGATCCCTGGCCTCATTCAGTG2760.2302897813081461No Hit
GTAATGGGGGCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACA2700.22528348171449072No Hit
GTAATGGGGGAGAAGCTCCAGCTTTGCTTTCTGCCCAGCCCATCTTGCCTGC2680.2236147151832723No Hit
GTAATGGGGGGCTCATCTGCAGCTCTAGCCTGGATTCTGCCCCAACCCCAAA2640.2202771821208354No Hit
GTAATGGGGAGGCACTGATGCTTCTGTGCTGTTTCCATTCTGTGATCATGTC2610.21777403232400772No Hit
GTAATGGGGACCAGCCTCTGACACGCGCCAGACTGCACAGAGGCCCTGCAGC2590.21610526579278924No Hit
GTAATGGGGAGTAACTATTGGGCAGCTTTGGCTCTTCGTCTACACCAGGGAC2500.20859581640230623No Hit
GTAATGGGAAACAGTGGTATCAACGTGCAGTGCTGTAATGGGGAGTGCAGTG2470.2060926666054786No Hit
GTAATGGGGCACTGTCTCATGTTGTGCTTCTTAAACGACAAAGAACATAGTG2440.2035895168086509No Hit
GTAATGGGGGAGGGCACCAAGATGGCGGCGCGCGCAGCGTGTGTGGCTCTGT2440.2035895168086509No Hit
GTAATGGGGGTTCTACTGTGACAGTGTGATTGCGCAGTGAGGAGGGGGCTGG2410.2010863670118232No Hit
GTAATGGGGATCGCTGTCCTCTGAGGCTTGGGTTCAGCGTTTGAATGCTTGA2360.19691445068377708No Hit
GTAATGGGGGAATATGTTACGAACAAATGTTTTAATAACAGTTGGCTGTAAT2360.19691445068377708No Hit
GTAATGGAGGAGGCAATGTTGCTGGTGACAGCAAAAACGACCCACCAATGGA2340.19524568415255864No Hit
GTAATGGGGTAGATTAAGGTGCCCAGGAGTCCTCTGACCAGGGAAGTGTTAG2310.19274253435573097No Hit
GTAATGGGGAAGAGTGTGCTAAACTATTTTCTAAAAATTACTTGTGCATTTT2200.18356431843402948No Hit
GTAATGGGGCACGAGACTCAGGGTGACCAGCAGCCCCAGCGCCTCGGACGAT2200.18356431843402948No Hit
GTAATGGGGTACAAGCCCCTAGACCTGCGACCCAAGAAGACTAGAGCCATGC2180.18189555190281104No Hit
GTAATGGGGCCGACGCCAATAGCGACGACCAAGATTTCCGGTTTCAGGTGAG2170.1810611686372018No Hit
GTAATGGGGGGTGACCCCATCGGTCCCCCCGCAACGATTACCCGTGAACCTG2170.1810611686372018No Hit
GTAATGGGGTGCAGGGAGTGGGAGCCGGGTGGACATCTGAGGCTGGGGCCAG2160.1802267853715926No Hit
GTAATGGGGGCTCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAG2160.1802267853715926No Hit
GTAATGGGGCAAATGCAGTTTAGCATATTCAGTCTTGACTATAAAGATTTGT2130.17772363557476492No Hit
GTAATGGGGATTTTAAAGGATTAAATCATGTGTCCTCTGATACATAAGCAAG2130.17772363557476492No Hit
GTAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2130.17772363557476492No Hit
GTAATGGGGATTTGGAATGGAGGCTCCTTCTTATATAAACTGAAGGCATGAC2090.17438610251232803No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAA2080.17355171924671878No Hit
GTAATGGGGAGAAAGGGCAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAG2080.17355171924671878No Hit
GTAATGGGGGACTGCTTCGACCTCTGCCGCCGCCACTGCCGCCGCCTCGGGA2060.17188295271550033No Hit
GTAATGGGGGTATGACCTTGGTGCTGTGTGCCTGCTGTTGACTCCTAGGACT2050.1710485694498911No Hit
GTAATGGGGGTAGAAAATATTTTTTTCTCTGCCATTTATTTGGGAAAAAGTA2030.16937980291867266No Hit
GTAATGGGGGGTTTGCTGGAACAAGGTGTGCTCGCCGGTCAGGGCCAGGAAC1970.16437350332501732No Hit
GTAATGGGGGCCGCTCGGTCTCGGCCAGTCGTTTGAACAGCGCCATCAATGC1960.1635391200594081No Hit
GTAATGGGGGTGAAGTGTACTTACTTTTAATAAAAAGGATATTGTTTGGAAG1960.1635391200594081No Hit
GTAATGGGGAGGCTGCTGGGCACTGGTTGGCTCTGTTATGCTGCGTGGCCCC1940.16187035352818965No Hit
GTAATGGGGGGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1940.16187035352818965No Hit
GTAATGGGGGTACTGTATGTGTTAATTTCTACTTTTTTAATATTTAAAATTG1910.15936720373136196No Hit
GTAATGGGGGATCTCCCTAGCCCTATGGCTGGGCACAGTGGGCACACGTGGG1880.1568640539345343No Hit
GTAATGGGGGGAGGTCTGGGCGGGAGCGGCCGAGTGGCGCGAGGAGCCGTTA1870.15602967066892506No Hit
GTAATGGGGATTGTTACCCAGGGCAGGGGTACAGGGGAAGCTGCAGCTATTA1850.15436090413770662No Hit
GTAATGTGGGGAAGGGGCGGGGCTTCCTCGGCAAGATGGCGGCCGCGGCCAC1840.1535265208720974No Hit
GTAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1820.15185775434087895No Hit
GTAATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.15185775434087895No Hit
GTAATGGGGAGTTTGCAAGAATCTCCCCGTTCCTTCTCTCTGATGCAGGCAC1790.14935460454405125No Hit
GTAATGGGGTATGAAACTCCTACTGGAGCTCAATTTGTAATTTAGAAACATT1770.1476858380128328No Hit
GTAATGGGGTGACTGTTCTTTCCCAGATGAAGTCTAGTGCCAATTTTGTCTT1750.14601707148161436No Hit
GTAATGGGGGTAATCTCGGTGGCATCCTCTAAGCCACTTAGACTGGCACACA1750.14601707148161436No Hit
GTAATGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC1720.1435139216847867No Hit
GTAATGGGGGGTAGGAGATAACCAGTCTTGTTTGTTTCTTACATGGCATTCC1710.14267953841917747No Hit
GTAATGGGGATCTTGACCCATATCTGTACTATTGCAGATGGTCACTCTGAAG1710.14267953841917747No Hit
GTAATGGGGACGCTACCACGTCTCAGAAAGCCTGGAGAGAAGAGTTTGTATC1670.13934200535674057No Hit
GTAATGGGGATTCCGAGTGGAGCCTCTGTTCTGATTGATCTTCAGGGCCCTG1660.13850762209113135No Hit
GTAATGGGGACTTTTCATTCTTTCTGTTTATTCATATAACTCATTATTGTGC1640.13683885555991288No Hit
GTAATGGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT1640.13683885555991288No Hit
GTAATGGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG1640.13683885555991288No Hit
GTAATGGGGTGCTGCCGTTCGGTCTGGCGCACCTTCCGGCACGAAATACACC1610.13433570576308523No Hit
GTAATGGGGATTCTAAATCCAGTCACTTTAACAACGGAGATTAACCATCACA1610.13433570576308523No Hit
GTAATGGGGGCTTCACACTGACGCCGGAAGGGGATGAACCGCTTCCCGGTGC1600.13350132249747598No Hit
GTAATGGGGAACTGAAAATCACGGAAAATGAGAAATACACACATTAGGATGT1560.1301637894350391No Hit
GTAATGGGGGAAAGGGCAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGT1560.1301637894350391No Hit
GTAATGGGGATTTGTGTATCAACATGTGACCTTGGTCCCCCCTCTACCTCCT1560.1301637894350391No Hit
GTAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.12932940616942987No Hit
GTAATGGGACAGAGTGCAGTGCTGTAATGGGGGCCGGTAGTGGTGGCGCACG1540.12849502290382064No Hit
GTAATGGGGAAATTAATCATTTTCAAACTCGTACATACGAGTTATTGCTTTA1530.12766063963821142No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGCAGATCGGACAGACGCGCTTCT1530.12766063963821142No Hit
GTAATGGGGACAGCTTACCTGGCTTGGAGTTCAGGTAATGGTGGCCATCCTG1530.12766063963821142No Hit
GTAATGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1520.1268262563726022No Hit
GTAATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.12599187310699297No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAA1500.12515748984138372No Hit
GTAATGGGGGTCAGACGTTACCGAGTCGCCCACGAGGTCCAGCCACATGGGC1500.12515748984138372No Hit
GTAATGGGGATCTGGCCTCTGCAGGATGGCTTTGCGGGCCTGTACTCCGCTG1500.12515748984138372No Hit
GTAATGGGGGATACTGGGTCGCCGTCGCCAGCTCCTGGATGTTCATCTGGAT1490.12432310657577451No Hit
GTAATGGGGAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.12432310657577451No Hit
GTAATGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA1480.12348872331016528No Hit
GTAATGGGGATGATCCGAAAGAACAAGTACCGCCCTGATCTGCGTATGGCGG1480.12348872331016528No Hit
GTAATGGGGGTTCCTCGCCGACACCAGCCAACTACTGTCTGCAGTGAAGGAG1470.12265434004455607No Hit
GTAATGGGGGATACACTTGTACTCTTTAGTTTGAGAAAATTATTTGGAATGT1460.12181995677894683No Hit
GTAATGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG1460.12181995677894683No Hit
GTAATGGGGGCTTTCCACCTCCCCAGATCCCTTTCTCTGGCCCAGTCTTTGG1460.12181995677894683No Hit
GTAATGGGGAGGAGAAGTGGTAGGTCTAGGTCCAGAACACCAGCCAGGAGAA1430.11931680698211916No Hit
GTAATGGGGGGTCTGGGTGCAGCCAGTGCAGCAGGATGCCGGAGCCTTCCCG1430.11931680698211916No Hit
GTAATGGGGTGGCTGGGATCAGAGGCTTTAAAATCTCTTTTCATCTTGGAGC1430.11931680698211916No Hit
GTAATGGGGGACAGTCACTGTAGTCCAGGATCACAGCCCATCATGGCCTAGA1410.11764804045090071No Hit
GTAATGGGGATGTGAGATGGCACTGGACTGAGAGACCTGGGAGCAAGCCCTG1400.1168136571852915No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGGAG1400.1168136571852915No Hit
GTAATGGGGAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAA1390.11597927391968227No Hit
GTAATGGGTACTGGGAGGTAGCAGAACCTTACGTAGGACCTAGTGGGAGGAA1360.1134761241228546No Hit
GTAATGGGATTTATCATTGTAAAACCAAGGACAATTTTATAACTTTTTTGTA1340.11180735759163615No Hit
GTAATGGGGATCTGTGGTTTTACATTTCTTTTATGGTTCTCAAGCTGGTGCA1340.11180735759163615No Hit
GTAATGGGGAGATACTTTTTACACTTTTACAAAATGTTGCACAAGAGGAAGC1330.11097297432602692No Hit
GTAATGGGGAGGGCTCAGTCTTCTCTCCCAGCTCCAGCAGCTGTGGAAGAGG1320.1101385910604177No Hit
GTAATGGGGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATAC1310.10930420779480847No Hit
GTAATGGGGAGCAACACAGATACTTTGTGGAGGCCTATGCCAAAGTTGGCCC1310.10930420779480847No Hit
GTAATGGGGAGTGCCAGTTTTCCTCCTCGCTCACCCCTTGTTTCACAATTAC1310.10930420779480847No Hit
GTAATGGGTGTTGGTCACAGTCCACTTTTTCGTAATGTTACTGTCTCTAAGT1290.10763544126359001No Hit
GTAATGGGGCTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGG1280.1068010579979808No Hit
GTAATGGGGATTGCTGGGATACCCAATGTACTGTTCCACTCCGGACATCTCA1270.10596667473237156No Hit
GTAATGGGGAACTCTGACTTCTTCAGCAACTCTCGGCTTGCCTCTCCTGCCC1260.10513229146676235No Hit
GTAATGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAA1260.10513229146676235No Hit
GTAATGGGGTAGCTATTGTCAGTTCTTATTCTGTGCATTCATTCTCAAGCCC1250.10429790820115312No Hit
GTAATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGAAGCA1250.10429790820115312No Hit
GTAATGGGGGGAACAGTGCCACAGTCTTATCTCAGAAAATTTAGAACTGCGG1250.10429790820115312No Hit
GTAATGGGGATCTCACCTGACTGCTTTCCTGCCCTCAGACCTGGACACTGGG1250.10429790820115312No Hit
GTAATGGGGGATTACTAAATCTTCAGTTTTTTTAAGCTTGTATTTTTGTACA1240.1034635249355439No Hit
GTAATGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.10262914166993466No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGCACGCTGCTGTCGATCATCGCG1220.10179475840432545No Hit
GTAATGGGGAGGGGTCCCACATTCTTGTGTAGTTTGCACAGGTACATGTGCA1220.10179475840432545No Hit
GTAATGGGGAGTCCTGACAGTGCAGAATCTGCATGTAAATTGTATATATCCT1220.10179475840432545No Hit
GTAATGGGGAAGGTAGATTTAAGTAATAAAGATAAAAGTACTAAAATATTGT1210.10096037513871621No Hit
GTAATGGGGGTTTTTCTTTCTCCAGCAGCCGAGCAAGATGCCCAAAGGGAAG1210.10096037513871621No Hit
GTAATGGGGGGAGGCCCAGTGGGCAGAGGTGATACAAACAGGCATGGCAGAC1210.10096037513871621No Hit
GTAATGAGGATCCTTTGCTTCCTCTTTGGCAACCCCAGCCTCGCTCTGGCTC1200.100125991873107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAG301.8473784E-646.033
CTTTTGA501.6370905E-1146.017
CTTCATG206.2887295E-446.046
GCCCTAT253.3987337E-546.020
ACTGCTC405.5388227E-946.046
ATCCCGA301.8473784E-646.021
ACTGTTG253.3987337E-546.030
CAGCTAT206.2887295E-446.044
TAGAAGT253.3987337E-546.029
CTTTTAC301.8473784E-646.024
ACAGCCC301.8473784E-646.033
CGACACC453.0559022E-1046.019
GGATGTT206.2887295E-446.037
GAGGCGT253.3987337E-546.020
ACAGTCA301.8473784E-646.011
CTCTGAG501.6370905E-1146.019
AACGTTC253.3987337E-546.020
TAGCGGG301.8473784E-646.043
GCGGGCG301.8473784E-646.031
TTTGAAT453.0559022E-1046.040
Produced by FastQC (version 0.11.5)