FastQCFastQC Report
Fri 17 Jun 2016
SRR1527313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117589
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40783.4680114636573145No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG17661.5018411586117748No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13391.1387119543494715No Hit
GTAATGGGGGGAGGCCATCAACTACATGGCGGCGGATGGAGACTTCAAGATT6880.5850887412938285No Hit
GTAATGGGGATTTTAAGATTTTGGTAAAAGGATACTATAAAAACAATGTTTG6400.5442685965523987No Hit
GTAATGGGGGACTGTACTGGCCAGTGTCCTTTACTTTCCCTCCTGACAGATA5510.46858124484433067No Hit
GTAATGGGGGTGGCCAATGCATCCCGAGCAGCGGGCGGCTTCCCATGACTCC5290.4498720118378419No Hit
GTAATGGGGATCCCTAGGAAGGATGAAATTTGAGAGGTGATCATGTCTTTTT5210.44306865438093695No Hit
GTAATGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGC4660.3962955718647152No Hit
GTAATGGGGATGGCATCAGGCTTTGGCAGATAGTGTCAGAGCTGTGTGTGTG4630.39374431281837585No Hit
GTAATGGGGGTTAGGAGGAAGACGGAAGCTCCAGCCGGGCTTTGGGACTGGC4490.38183843726879213No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4390.3733342404476609No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4330.3682317223549822No Hit
GTAATGGGGCTTCACCTACCCTGAAGCTGACTCCTTCCCAAGCTGTGCCGCT4310.3665308829907559No Hit
GTAATGGGGATCTTGACTTTTGATATTTTTTGGGGGGGTGGGGGTGGGCATC4130.3512233287127198No Hit
GTAATGGGGGCTCACGTCGCTGCCCGCTGATCGACGATGCGGTAATCCGTTT4080.3469712303021541No Hit
GTAATGGGGGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCC4010.3410182925273622No Hit
GTAATGGAGAGAAAAAATTTTTAGGTTTATTATTTTTTTATTTATATTTTTA4000.34016787284524913No Hit
GTAATGGGGACATTTACTTATTTTGTGTGTGCAGATGCTCTAGCAGACATGT3900.3316636760241179No Hit
GTAATGGGGGGGCTGTTTGAGAAATAATGTGAGAGCAAATGTTAAAAGAAAG3860.3282619972956654No Hit
GTAATGGGGTCTGTAGCACCTCCTGGCAGCACGCAGTTAAACCAGATCTACC3730.3172065414281948No Hit
GTAATGGGGACCGTCGTTCCCCAGCTGATGAGTTCTGAATTCGGCAGTTGAA3220.27383513764042555No Hit
GTAATGGGGACGCAGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAA3030.2576771636802762No Hit
GTAATGGGGATTTGTTTCTGGTTTAGCCTCAAGGGCTGGAAGCTGGGCCTGT2970.25257464558759746No Hit
GTAATGGGGAAGTTTTTAGGTATTTAATTTGTAAAACAGTTTGCTTTGTACT2810.23896793067378752No Hit
GTAATGGGGAGAAAGAGCAGCACAGCTGGCCATCAGAGTCACCAATCCCAAC2630.2236603763957513No Hit
GTAATGGGGATCAAGACTATCTACAAAAATTTATTATTTACAGAAAAAAGCA2630.2236603763957513No Hit
GTAATGGGGGGCTCATCTGCAGCTCTAGCCTGGATTCTGCCCCAACCCCAAA2600.22110911734941194No Hit
GTAATGGGGAGTAACTATTGGGCAGCTTTGGCTCTTCGTCTACACCAGGGAC2590.2202586976672988No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAA2470.21005366148194132No Hit
GTAATGGGGAAGAGTGTGCTAAACTATTTTCTAAAAATTACTTGTGCATTTT2460.2092032417998282No Hit
GTAATGGGGGAGAAGCTCCAGCTTTGCTTTCTGCCCAGCCCATCTTGCCTGC2450.2083528221177151No Hit
GTAATGGGGGAATATGTTACGAACAAATGTTTTAATAACAGTTGGCTGTAAT2450.2083528221177151No Hit
GTAATGGGGATCGCTGTCCTCTGAGGCTTGGGTTCAGCGTTTGAATGCTTGA2390.20325030402503635No Hit
GTAATGGGGCACTGTCTCATGTTGTGCTTCTTAAACGACAAAGAACATAGTG2380.20239988434292322No Hit
GTAATGGGGAGGCACTGATGCTTCTGTGCTGTTTCCATTCTGTGATCATGTC2370.20154946466081008No Hit
GTAATGGGGGCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACA2370.20154946466081008No Hit
GTAATGGGGGTATCCATGAGAATGTGGGTTTGATCCCTGGCCTCATTCAGTG2340.19899820561447074No Hit
GTAATGGGGCCGACGCCAATAGCGACGACCAAGATTTCCGGTTTCAGGTGAG2330.1981477859323576No Hit
GTAATGGGGCACGAGACTCAGGGTGACCAGCAGCCCCAGCGCCTCGGACGAT2280.193895687521792No Hit
GTAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2280.193895687521792No Hit
GTAATGGGAAACAGTGGTATCAACGTGCAGTGCTGTAATGGGGAGTGCAGTG2270.19304526783967887No Hit
GTAATGGGGGTTCTACTGTGACAGTGTGATTGCGCAGTGAGGAGGGGGCTGG2230.1896435891112264No Hit
GTAATGGGGGGTGACCCCATCGGTCCCCCCGCAACGATTACCCGTGAACCTG2210.18794274974700015No Hit
GTAATGGGGACCAGCCTCTGACACGCGCCAGACTGCACAGAGGCCCTGCAGC2200.187092330064887No Hit
GTAATGGGGGACTGCTTCGACCTCTGCCGCCGCCACTGCCGCCGCCTCGGGA2180.18539149070066077No Hit
GTAATGGAGGAGGCAATGTTGCTGGTGACAGCAAAAACGACCCACCAATGGA2170.18454107101854766No Hit
GTAATGGGGTGCAGGGAGTGGGAGCCGGGTGGACATCTGAGGCTGGGGCCAG2170.18454107101854766No Hit
GTAATGGGGGTATGACCTTGGTGCTGTGTGCCTGCTGTTGACTCCTAGGACT2090.17773771356164267No Hit
GTAATGGGGGGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2060.17518645451530332No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAA2030.17263519546896394No Hit
GTAATGGGGGAGGGCACCAAGATGGCGGCGCGCGCAGCGTGTGTGGCTCTGT2030.17263519546896394No Hit
GTAATGGGGGCTCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAG2020.1717847757868508No Hit
GTAATGGGGTAGATTAAGGTGCCCAGGAGTCCTCTGACCAGGGAAGTGTTAG1970.1675326773762852No Hit
GTAATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.1649814183299458No Hit
GTAATGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC1920.16328057896571957No Hit
GTAATGGGGAGAAAGGGCAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAG1910.16243015928360646No Hit
GTAATGGGGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.15732764119092774No Hit
GTAATGGGGACGCTACCACGTCTCAGAAAGCCTGGAGAGAAGAGTTTGTATC1840.1564772215088146No Hit
GTAATGGGGGGTTTGCTGGAACAAGGTGTGCTCGCCGGTCAGGGCCAGGAAC1830.1556268018267015No Hit
GTAATGGGGCAAATGCAGTTTAGCATATTCAGTCTTGACTATAAAGATTTGT1820.15477638214458836No Hit
GTAATGGGGAGGCTGCTGGGCACTGGTTGGCTCTGTTATGCTGCGTGGCCCC1820.15477638214458836No Hit
GTAATGGGGGTAATCTCGGTGGCATCCTCTAAGCCACTTAGACTGGCACACA1820.15477638214458836No Hit
GTAATGGGGGGAGGTCTGGGCGGGAGCGGCCGAGTGGCGCGAGGAGCCGTTA1810.15392596246247525No Hit
GTAATGGGACAGAGTGCAGTGCTGTAATGGGGGCCGGTAGTGGTGGCGCACG1790.15222512309824898No Hit
GTAATGGGGGCCGCTCGGTCTCGGCCAGTCGTTTGAACAGCGCCATCAATGC1780.15137470341613585No Hit
GTAATGGGGATTTGGAATGGAGGCTCCTTCTTATATAAACTGAAGGCATGAC1770.15052428373402274No Hit
GTAATGGGGGATACACTTGTACTCTTTAGTTTGAGAAAATTATTTGGAATGT1760.1496738640519096No Hit
GTAATGGGGGAAAGGGCAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGT1760.1496738640519096No Hit
GTAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1750.1488234443697965No Hit
GTAATGGGGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAA1750.1488234443697965No Hit
GTAATGGGGGTACTGTATGTGTTAATTTCTACTTTTTTAATATTTAAAATTG1740.14797302468768336No Hit
GTAATGGGGTACAAGCCCCTAGACCTGCGACCCAAGAAGACTAGAGCCATGC1720.14627218532345712No Hit
GTAATGGGGGCTTCACACTGACGCCGGAAGGGGATGAACCGCTTCCCGGTGC1710.145421765641344No Hit
GTAATGGGGATGATCCGAAAGAACAAGTACCGCCCTGATCTGCGTATGGCGG1710.145421765641344No Hit
GTAATGGGGAGGGCTCAGTCTTCTCTCCCAGCTCCAGCAGCTGTGGAAGAGG1680.14287050659500464No Hit
GTAATGGGGGACAGTCACTGTAGTCCAGGATCACAGCCCATCATGGCCTAGA1680.14287050659500464No Hit
GTAATGGGGAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAA1640.13946882786655215No Hit
GTAATGGGGATTGTTACCCAGGGCAGGGGTACAGGGGAAGCTGCAGCTATTA1620.1377679885023259No Hit
GTAATGGGGGTCAGACGTTACCGAGTCGCCCACGAGGTCCAGCCACATGGGC1620.1377679885023259No Hit
GTAATGGGGGCTTTCCACCTCCCCAGATCCCTTTCTCTGGCCCAGTCTTTGG1610.13691756882021278No Hit
GTAATGGGGATTTTAAAGGATTAAATCATGTGTCCTCTGATACATAAGCAAG1610.13691756882021278No Hit
GTAATGGGGATTTGTGTATCAACATGTGACCTTGGTCCCCCCTCTACCTCCT1590.13521672945598653No Hit
GTAATGGGGGTGAAGTGTACTTACTTTTAATAAAAAGGATATTGTTTGGAAG1590.13521672945598653No Hit
GTAATGGGGACTTTTCATTCTTTCTGTTTATTCATATAACTCATTATTGTGC1580.13436630977387343No Hit
GTAATGGGGATTCCGAGTGGAGCCTCTGTTCTGATTGATCTTCAGGGCCCTG1580.13436630977387343No Hit
GTAATGGGGATTCTAAATCCAGTCACTTTAACAACGGAGATTAACCATCACA1580.13436630977387343No Hit
GTAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.13436630977387343No Hit
GTAATGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1570.1335158900917603No Hit
GTAATGGGGGTAGAAAATATTTTTTTCTCTGCCATTTATTTGGGAAAAAGTA1560.13266547040964716No Hit
GTAATGGGGATCTGGCCTCTGCAGGATGGCTTTGCGGGCCTGTACTCCGCTG1530.13011421136330778No Hit
GTAATGGGGATCTTGACCCATATCTGTACTATTGCAGATGGTCACTCTGAAG1510.12841337199908154No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAA1510.12841337199908154No Hit
GTAATGGGGTGGCTGGGATCAGAGGCTTTAAAATCTCTTTTCATCTTGGAGC1490.1267125326348553No Hit
GTAATGGGGATGTGAGATGGCACTGGACTGAGAGACCTGGGAGCAAGCCCTG1470.12501169327062905No Hit
GTAATGAGGATCCTTTGCTTCCTCTTTGGCAACCCCAGCCTCGCTCTGGCTC1470.12501169327062905No Hit
GTAATGGGGAGTTTGCAAGAATCTCCCCGTTCCTTCTCTCTGATGCAGGCAC1470.12501169327062905No Hit
GTAATGGGGGATCTCCCTAGCCCTATGGCTGGGCACAGTGGGCACACGTGGG1460.12416127358851592No Hit
GTAATGGGGAGCAACACAGATACTTTGTGGAGGCCTATGCCAAAGTTGGCCC1450.12331085390640281No Hit
GTAATGGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT1440.12246043422428968No Hit
GTAATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.12161001454217657No Hit
GTAATGGGGGGTAGGAGATAACCAGTCTTGTTTGTTTCTTACATGGCATTCC1420.12075959486006344No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGCAGATCGGACAGACGCGCTTCT1420.12075959486006344No Hit
GTAATGGGGAACTGAAAATCACGGAAAATGAGAAATACACACATTAGGATGT1400.11905875549583719No Hit
GTAATGGGGATTGCTGGGATACCCAATGTACTGTTCCACTCCGGACATCTCA1400.11905875549583719No Hit
GTAATGGGGACAGCTTACCTGGCTTGGAGTTCAGGTAATGGTGGCCATCCTG1390.11820833581372409No Hit
GTAATGGGGTGACTGTTCTTTCCCAGATGAAGTCTAGTGCCAATTTTGTCTT1380.11735791613161095No Hit
GTAATGTGGGGAAGGGGCGGGGCTTCCTCGGCAAGATGGCGGCCGCGGCCAC1370.11650749644949783No Hit
GTAATGGGGAAATTAATCATTTTCAAACTCGTACATACGAGTTATTGCTTTA1350.11480665708527159No Hit
GTAATGGGGGTGAATCTCTATTTGTCATTTTGTATTTGTATATTAAAATTAA1340.11395623740315845No Hit
GTAATGGGGAACTCTGACTTCTTCAGCAACTCTCGGCTTGCCTCTCCTGCCC1330.11310581772104535No Hit
GTAATGGGGAGGAGAAGTGGTAGGTCTAGGTCCAGAACACCAGCCAGGAGAA1320.11225539803893221No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGGAG1320.11225539803893221No Hit
GTAATGGGGGGTCTGGGTGCAGCCAGTGCAGCAGGATGCCGGAGCCTTCCCG1310.1114049783568191No Hit
GTAATGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA1310.1114049783568191No Hit
GTAATGGGGGATACTGGGTCGCCGTCGCCAGCTCCTGGATGTTCATCTGGAT1310.1114049783568191No Hit
GTAATGGGGCTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGG1300.11055455867470597No Hit
GTAATGGGGGTTCCTCGCCGACACCAGCCAACTACTGTCTGCAGTGAAGGAG1290.10970413899259283No Hit
GTAATGGGGAGATACTTTTTACACTTTTACAAAATGTTGCACAAGAGGAAGC1280.10885371931047973No Hit
GTAATGGGGTATGAAACTCCTACTGGAGCTCAATTTGTAATTTAGAAACATT1280.10885371931047973No Hit
GTAATGGGGGTTGATTTGTGTTCTGTGTCTGTTTTGGCTCAGTTTCCTTACC1280.10885371931047973No Hit
GTAATGGGGGGTCATTAGGCGAGTAGACAGGAGAGTCCCCGCATTGACCAGC1280.10885371931047973No Hit
GTAATGGGGGGATCAGTATAGTAAAAATGTCCATCTTGCTGAAAGCAATCTA1270.10800329962836659No Hit
GTAATGGGGAGGGGTCCCACATTCTTGTGTAGTTTGCACAGGTACATGTGCA1270.10800329962836659No Hit
GTAATGGGGTAATCACTGAGTTGTTTATAAAAGACACATCAAACTTTTCAAG1260.10715287994625347No Hit
GTAATGGGGTGCTGCCGTTCGGTCTGGCGCACCTTCCGGCACGAAATACACC1250.10630246026414035No Hit
GTAATGGGATTTATCATTGTAAAACCAAGGACAATTTTATAACTTTTTTGTA1250.10630246026414035No Hit
GTAATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGAAGCA1250.10630246026414035No Hit
GTAATGGGGTTGATAATATCGAAGAGTGTGAAGAAAGGCTTGCACAAGCCGA1230.1046016208999141No Hit
GTAATGGGGACTAAAATCAGACTGCAAAATTCTTGTCCTGCAGGAATGGGAC1230.1046016208999141No Hit
GTAATGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG1220.10375120121780099No Hit
GTAATGGGGCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGTCACGG1220.10375120121780099No Hit
GTAATGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1220.10375120121780099Illumina Single End Adapter 2 (97% over 34bp)
GTAATGGGGGACTGTCAGTCCTGGAGAATTAGAACATGCCTGCTAAATTTCA1220.10375120121780099No Hit
GTAATGGGGGTTTTTCTTTCTCCAGCAGCCGAGCAAGATGCCCAAAGGGAAG1190.10119994217146161No Hit
GTAATGGGGTGAACACTCAAGCTAACCAACAGCTCTTTAGCAGAAATTTAAG1190.10119994217146161No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC1190.10119994217146161No Hit
GTAATGGGGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATAC1180.1003495224893485No Hit
GTAATGGGGGATTACTAAATCTTCAGTTTTTTTAAGCTTGTATTTTTGTACA1180.1003495224893485No Hit
GTAATGGAGAGAGCTGGAATGTAACGTTGCTTACAAGCATTGCTATTCAACT1180.1003495224893485No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTC1180.1003495224893485No Hit
GTAATGGGGATCTGTGGTTTTACATTTCTTTTATGGTTCTCAAGCTGGTGCA1180.1003495224893485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGG351.00912075E-746.00000438
ACTGCTC351.00912075E-746.00000446
CGTTCCC351.00912075E-746.00000415
TTATGCT351.00912075E-746.00000436
GGATGAC351.00912075E-746.0000048
TGGCAAC351.00912075E-746.00000427
GTGTCAG351.00912075E-746.00000433
TCAAGGG351.00912075E-746.00000429
TAAACGA351.00912075E-746.00000432
CACACCC351.00912075E-746.00000425
GCCCAAC700.046.00000415
TCTAGCA351.00912075E-746.00000439
GAAACCC700.046.00000446
TGCGTGG351.00912075E-746.00000442
TGCGCAG351.00912075E-746.00000431
TTACCCG351.00912075E-746.00000438
AATTCGG351.00912075E-746.00000438
TGTCTCA351.00912075E-746.00000413
CGGGGCT351.00912075E-746.00000418
CCCGGCG351.00912075E-746.00000429
Produced by FastQC (version 0.11.5)