##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527313_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117589
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91851278605992	33.0	31.0	34.0	30.0	34.0
2	30.94189932731803	31.0	31.0	33.0	28.0	34.0
3	31.70854416654619	31.0	31.0	34.0	30.0	34.0
4	36.15350925681824	37.0	35.0	37.0	35.0	37.0
5	36.16334010834347	37.0	35.0	37.0	35.0	37.0
6	36.20166852341631	37.0	36.0	37.0	35.0	37.0
7	36.56447456819941	37.0	37.0	37.0	35.0	37.0
8	36.58568403507131	37.0	37.0	37.0	35.0	37.0
9	38.28243288062659	39.0	39.0	39.0	37.0	39.0
10	37.60585598993103	39.0	37.0	39.0	35.0	39.0
11	36.99687045556983	39.0	35.0	39.0	34.0	39.0
12	36.975346333415544	39.0	37.0	39.0	33.0	39.0
13	36.816598491355485	39.0	37.0	39.0	33.0	39.0
14	37.80970158773355	40.0	37.0	40.0	33.0	40.0
15	37.88407929313116	40.0	37.0	40.0	33.0	40.0
16	37.757137147182135	40.0	37.0	40.0	33.0	40.0
17	37.95375417768669	40.0	37.0	40.0	34.0	40.0
18	37.90538230616809	40.0	37.0	40.0	34.0	40.0
19	37.53338322462135	40.0	37.0	40.0	33.0	40.0
20	37.85221406764238	40.0	37.0	40.0	34.0	40.0
21	33.6110350457951	39.0	34.0	40.0	10.0	40.0
22	34.457406730221365	39.0	34.0	40.0	17.0	40.0
23	36.42931736812117	39.0	34.0	40.0	30.0	40.0
24	37.07667383853932	39.0	36.0	40.0	32.0	40.0
25	37.08961722610108	39.0	35.0	40.0	33.0	40.0
26	36.876604104125384	39.0	35.0	40.0	33.0	40.0
27	36.87562612149095	39.0	35.0	40.0	33.0	40.0
28	36.75889751592411	39.0	35.0	40.0	32.0	40.0
29	36.72818035700618	39.0	35.0	40.0	33.0	40.0
30	36.3089574705117	38.0	35.0	40.0	31.0	40.0
31	36.15289695464712	38.0	35.0	40.0	31.0	40.0
32	35.618637797753195	38.0	35.0	40.0	30.0	40.0
33	35.34966706069445	38.0	35.0	40.0	27.0	40.0
34	35.20491712660198	38.0	35.0	40.0	26.0	40.0
35	35.25807686093087	38.0	35.0	40.0	27.0	40.0
36	34.873942290520375	38.0	35.0	40.0	24.0	40.0
37	34.20970498941227	38.0	34.0	40.0	21.0	40.0
38	33.91738172788271	37.0	33.0	40.0	19.0	40.0
39	33.67141484322513	37.0	33.0	40.0	17.0	40.0
40	29.060873040845657	35.0	15.0	40.0	8.0	40.0
41	30.101319000926956	35.0	19.0	40.0	10.0	40.0
42	31.419962751617923	35.0	28.0	39.0	12.0	40.0
43	32.36642032843208	35.0	31.0	39.0	12.0	40.0
44	32.86107544073	35.0	33.0	40.0	13.0	40.0
45	32.867113420473004	35.0	33.0	40.0	12.0	40.0
46	32.8311321637228	35.0	33.0	40.0	11.0	40.0
47	32.77665427888663	35.0	33.0	40.0	10.0	40.0
48	32.67627073961	35.0	33.0	40.0	10.0	40.0
49	32.41616137563888	35.0	33.0	40.0	10.0	40.0
50	32.08679383275646	35.0	32.0	40.0	10.0	40.0
51	31.898349335397018	35.0	32.0	40.0	9.0	40.0
52	31.452304212128684	35.0	31.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	4.0
16	5.0
17	18.0
18	34.0
19	106.0
20	164.0
21	281.0
22	469.0
23	725.0
24	1130.0
25	1651.0
26	2387.0
27	3261.0
28	3572.0
29	3271.0
30	3202.0
31	3714.0
32	4549.0
33	5377.0
34	10182.0
35	10968.0
36	16824.0
37	13124.0
38	21987.0
39	10583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.86639056374321	0.561276990194661	0.415855224553317	0.1564772215088146
8	97.14599154682836	1.9687215640918794	0.3571762664875116	0.5281106225922493
9	90.05434181768703	6.390903911080118	2.2519113182355492	1.3028429529973042
10	33.21484152429224	52.16389288113684	7.579790626674264	7.041474967896658
11	23.859374601365772	42.082167549685764	23.20200018709233	10.856457661856126
12	33.53204806572043	30.75542780362109	20.124331357524937	15.58819277313354
13	19.7977701995935	33.975116720101376	22.201906640927298	24.025206439377833
14	21.509665019687215	41.49367712966349	20.81062004099023	16.18603780965907
15	28.02473020435585	35.162302596331294	20.836983051135736	15.975984148177124
16	19.73739040216347	38.10050259803213	26.1878236909915	15.9742833088129
17	29.29355637006863	31.901793535109572	20.268052283802056	18.53659781101974
18	25.5330005357644	32.22240175526622	24.082184558079412	18.162413150889964
19	21.646582588507428	33.628145489799216	29.04098172448103	15.684290197212325
20	21.256239954417506	40.67727423483489	20.932230055532404	17.1342557552152
21	26.434445398804314	30.417811189822174	27.31377935010928	15.833964061264233
22	19.041747102194932	35.04494468019968	20.89226033047309	25.021047887132298
23	17.43700516204747	42.9070746413355	22.348178826250756	17.307741370366276
24	18.721138882038286	41.98351886656065	21.52497257396525	17.770369677435816
25	22.331170432608495	35.528833479322046	21.784350577009754	20.35564551105971
26	29.72471914889998	33.980219238194046	21.7613892455927	14.533672367313269
27	21.670394339606595	34.260007313609265	21.605762443766	22.46383590301814
28	22.28184609104593	40.68407759229179	23.162880881715125	13.871195434947147
29	28.700813851635782	34.03804777657774	21.007917407240473	16.253220964546003
30	19.977208752519367	40.43915672384322	23.080390172550153	16.50324435108726
31	26.623238568233422	34.73709275527473	22.851627278061724	15.788041398430124
32	21.087006437676994	37.91511110733147	25.414792199950675	15.583090255040863
33	28.2160746328313	37.656583523969076	20.376906003112534	13.750435840087082
34	20.868448579373922	37.92106404510626	19.7458945989846	21.46459277653522
35	19.17866467101515	43.05249640697684	18.656506986197687	19.112331935810325
36	23.619556251009875	37.74077507249828	22.952827220233186	15.686841456258662
37	21.624471676772487	38.785940861815305	22.040326901325805	17.549260560086402
38	25.671618943948836	36.30441622940921	22.39069981035641	15.633265016285536
39	22.46723758174659	36.13773397171504	20.868448579373922	20.526579867164447
40	21.340431502946704	40.88647747663472	20.29951781204024	17.473573208378333
41	26.655554516153725	35.37490751685957	23.116958218881017	14.85257974810569
42	21.32852562739712	38.36838479789776	23.16628256044358	17.13680701426154
43	21.280051705516673	40.20359047189788	20.744287305785406	17.77207051680004
44	19.687215640918794	44.42762503295376	19.877709649712134	16.007449676415312
45	21.991002559763242	41.2479058415328	23.770080534743894	12.991011063960064
46	25.432651013275056	39.066579356912634	19.619182066349744	15.881587563462569
47	24.22590548435653	40.42810126797575	18.905679953056833	16.44031329461089
48	25.562765224638355	38.676236722822715	18.002534250652698	17.758463801886233
49	23.481788262507546	37.229672843548286	21.34128192262882	17.947256971315344
50	19.592819056204235	43.07885941712235	21.27154750869554	16.056774017977872
51	20.26380018539149	40.803136347787635	22.574390461692843	16.35867300512803
52	23.349973211780014	37.71696332139911	19.350449446801996	19.58261402001888
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.0
4	10.0
5	17.5
6	25.0
7	836.0
8	1647.0
9	3203.5
10	4760.0
11	3163.5
12	1567.0
13	1729.0
14	1430.0
15	969.0
16	889.5
17	810.0
18	825.0
19	840.0
20	922.0
21	1004.0
22	1203.5
23	1403.0
24	2035.5
25	2668.0
26	3095.5
27	3523.0
28	3643.5
29	3764.0
30	3873.0
31	3982.0
32	3858.0
33	3734.0
34	3617.5
35	3501.0
36	3533.0
37	3565.0
38	3745.0
39	4362.5
40	4800.0
41	4513.5
42	4227.0
43	4249.5
44	4272.0
45	3840.0
46	3408.0
47	3564.5
48	3721.0
49	5665.0
50	7609.0
51	9116.0
52	10623.0
53	9818.0
54	9013.0
55	7376.5
56	5740.0
57	5827.5
58	5915.0
59	4658.5
60	3402.0
61	3824.0
62	4246.0
63	3239.5
64	2077.0
65	1921.0
66	1651.0
67	1381.0
68	983.5
69	586.0
70	682.0
71	778.0
72	438.0
73	98.0
74	54.5
75	11.0
76	11.5
77	12.0
78	7.0
79	2.0
80	1.5
81	1.0
82	1.0
83	1.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	117589.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.613314170543166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67951295158232	23.595744499910705
2	8.626730228016772	5.109321450135642
3	3.5810694388604905	3.1814200307851923
4	1.9700189535351216	2.333551607718409
5	1.1372121072884958	1.6838309705839831
6	0.6777324679800126	1.204194269872182
7	0.5169145942220436	1.0715287994625349
8	0.4537361438171271	1.0749304781909874
9	0.2239963241628855	0.5969946168434122
>10	2.0561713859054622	13.809965217835002
>50	0.5714778013899259	12.200971179276973
>100	0.4824536212739073	25.538103053857082
>500	0.014358738728390098	2.4908792489093368
>1k	0.008615243237034059	6.108564576618561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4078	3.4680114636573145	No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG	1766	1.5018411586117748	No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1339	1.1387119543494715	No Hit
GTAATGGGGGGAGGCCATCAACTACATGGCGGCGGATGGAGACTTCAAGATT	688	0.5850887412938285	No Hit
GTAATGGGGATTTTAAGATTTTGGTAAAAGGATACTATAAAAACAATGTTTG	640	0.5442685965523987	No Hit
GTAATGGGGGACTGTACTGGCCAGTGTCCTTTACTTTCCCTCCTGACAGATA	551	0.46858124484433067	No Hit
GTAATGGGGGTGGCCAATGCATCCCGAGCAGCGGGCGGCTTCCCATGACTCC	529	0.4498720118378419	No Hit
GTAATGGGGATCCCTAGGAAGGATGAAATTTGAGAGGTGATCATGTCTTTTT	521	0.44306865438093695	No Hit
GTAATGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGC	466	0.3962955718647152	No Hit
GTAATGGGGATGGCATCAGGCTTTGGCAGATAGTGTCAGAGCTGTGTGTGTG	463	0.39374431281837585	No Hit
GTAATGGGGGTTAGGAGGAAGACGGAAGCTCCAGCCGGGCTTTGGGACTGGC	449	0.38183843726879213	No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	439	0.3733342404476609	No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	433	0.3682317223549822	No Hit
GTAATGGGGCTTCACCTACCCTGAAGCTGACTCCTTCCCAAGCTGTGCCGCT	431	0.3665308829907559	No Hit
GTAATGGGGATCTTGACTTTTGATATTTTTTGGGGGGGTGGGGGTGGGCATC	413	0.3512233287127198	No Hit
GTAATGGGGGCTCACGTCGCTGCCCGCTGATCGACGATGCGGTAATCCGTTT	408	0.3469712303021541	No Hit
GTAATGGGGGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCC	401	0.3410182925273622	No Hit
GTAATGGAGAGAAAAAATTTTTAGGTTTATTATTTTTTTATTTATATTTTTA	400	0.34016787284524913	No Hit
GTAATGGGGACATTTACTTATTTTGTGTGTGCAGATGCTCTAGCAGACATGT	390	0.3316636760241179	No Hit
GTAATGGGGGGGCTGTTTGAGAAATAATGTGAGAGCAAATGTTAAAAGAAAG	386	0.3282619972956654	No Hit
GTAATGGGGTCTGTAGCACCTCCTGGCAGCACGCAGTTAAACCAGATCTACC	373	0.3172065414281948	No Hit
GTAATGGGGACCGTCGTTCCCCAGCTGATGAGTTCTGAATTCGGCAGTTGAA	322	0.27383513764042555	No Hit
GTAATGGGGACGCAGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAA	303	0.2576771636802762	No Hit
GTAATGGGGATTTGTTTCTGGTTTAGCCTCAAGGGCTGGAAGCTGGGCCTGT	297	0.25257464558759746	No Hit
GTAATGGGGAAGTTTTTAGGTATTTAATTTGTAAAACAGTTTGCTTTGTACT	281	0.23896793067378752	No Hit
GTAATGGGGAGAAAGAGCAGCACAGCTGGCCATCAGAGTCACCAATCCCAAC	263	0.2236603763957513	No Hit
GTAATGGGGATCAAGACTATCTACAAAAATTTATTATTTACAGAAAAAAGCA	263	0.2236603763957513	No Hit
GTAATGGGGGGCTCATCTGCAGCTCTAGCCTGGATTCTGCCCCAACCCCAAA	260	0.22110911734941194	No Hit
GTAATGGGGAGTAACTATTGGGCAGCTTTGGCTCTTCGTCTACACCAGGGAC	259	0.2202586976672988	No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAA	247	0.21005366148194132	No Hit
GTAATGGGGAAGAGTGTGCTAAACTATTTTCTAAAAATTACTTGTGCATTTT	246	0.2092032417998282	No Hit
GTAATGGGGGAGAAGCTCCAGCTTTGCTTTCTGCCCAGCCCATCTTGCCTGC	245	0.2083528221177151	No Hit
GTAATGGGGGAATATGTTACGAACAAATGTTTTAATAACAGTTGGCTGTAAT	245	0.2083528221177151	No Hit
GTAATGGGGATCGCTGTCCTCTGAGGCTTGGGTTCAGCGTTTGAATGCTTGA	239	0.20325030402503635	No Hit
GTAATGGGGCACTGTCTCATGTTGTGCTTCTTAAACGACAAAGAACATAGTG	238	0.20239988434292322	No Hit
GTAATGGGGAGGCACTGATGCTTCTGTGCTGTTTCCATTCTGTGATCATGTC	237	0.20154946466081008	No Hit
GTAATGGGGGCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACA	237	0.20154946466081008	No Hit
GTAATGGGGGTATCCATGAGAATGTGGGTTTGATCCCTGGCCTCATTCAGTG	234	0.19899820561447074	No Hit
GTAATGGGGCCGACGCCAATAGCGACGACCAAGATTTCCGGTTTCAGGTGAG	233	0.1981477859323576	No Hit
GTAATGGGGCACGAGACTCAGGGTGACCAGCAGCCCCAGCGCCTCGGACGAT	228	0.193895687521792	No Hit
GTAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG	228	0.193895687521792	No Hit
GTAATGGGAAACAGTGGTATCAACGTGCAGTGCTGTAATGGGGAGTGCAGTG	227	0.19304526783967887	No Hit
GTAATGGGGGTTCTACTGTGACAGTGTGATTGCGCAGTGAGGAGGGGGCTGG	223	0.1896435891112264	No Hit
GTAATGGGGGGTGACCCCATCGGTCCCCCCGCAACGATTACCCGTGAACCTG	221	0.18794274974700015	No Hit
GTAATGGGGACCAGCCTCTGACACGCGCCAGACTGCACAGAGGCCCTGCAGC	220	0.187092330064887	No Hit
GTAATGGGGGACTGCTTCGACCTCTGCCGCCGCCACTGCCGCCGCCTCGGGA	218	0.18539149070066077	No Hit
GTAATGGAGGAGGCAATGTTGCTGGTGACAGCAAAAACGACCCACCAATGGA	217	0.18454107101854766	No Hit
GTAATGGGGTGCAGGGAGTGGGAGCCGGGTGGACATCTGAGGCTGGGGCCAG	217	0.18454107101854766	No Hit
GTAATGGGGGTATGACCTTGGTGCTGTGTGCCTGCTGTTGACTCCTAGGACT	209	0.17773771356164267	No Hit
GTAATGGGGGGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	206	0.17518645451530332	No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAA	203	0.17263519546896394	No Hit
GTAATGGGGGAGGGCACCAAGATGGCGGCGCGCGCAGCGTGTGTGGCTCTGT	203	0.17263519546896394	No Hit
GTAATGGGGGCTCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAG	202	0.1717847757868508	No Hit
GTAATGGGGTAGATTAAGGTGCCCAGGAGTCCTCTGACCAGGGAAGTGTTAG	197	0.1675326773762852	No Hit
GTAATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	194	0.1649814183299458	No Hit
GTAATGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC	192	0.16328057896571957	No Hit
GTAATGGGGAGAAAGGGCAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAG	191	0.16243015928360646	No Hit
GTAATGGGGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	185	0.15732764119092774	No Hit
GTAATGGGGACGCTACCACGTCTCAGAAAGCCTGGAGAGAAGAGTTTGTATC	184	0.1564772215088146	No Hit
GTAATGGGGGGTTTGCTGGAACAAGGTGTGCTCGCCGGTCAGGGCCAGGAAC	183	0.1556268018267015	No Hit
GTAATGGGGCAAATGCAGTTTAGCATATTCAGTCTTGACTATAAAGATTTGT	182	0.15477638214458836	No Hit
GTAATGGGGAGGCTGCTGGGCACTGGTTGGCTCTGTTATGCTGCGTGGCCCC	182	0.15477638214458836	No Hit
GTAATGGGGGTAATCTCGGTGGCATCCTCTAAGCCACTTAGACTGGCACACA	182	0.15477638214458836	No Hit
GTAATGGGGGGAGGTCTGGGCGGGAGCGGCCGAGTGGCGCGAGGAGCCGTTA	181	0.15392596246247525	No Hit
GTAATGGGACAGAGTGCAGTGCTGTAATGGGGGCCGGTAGTGGTGGCGCACG	179	0.15222512309824898	No Hit
GTAATGGGGGCCGCTCGGTCTCGGCCAGTCGTTTGAACAGCGCCATCAATGC	178	0.15137470341613585	No Hit
GTAATGGGGATTTGGAATGGAGGCTCCTTCTTATATAAACTGAAGGCATGAC	177	0.15052428373402274	No Hit
GTAATGGGGGATACACTTGTACTCTTTAGTTTGAGAAAATTATTTGGAATGT	176	0.1496738640519096	No Hit
GTAATGGGGGAAAGGGCAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGT	176	0.1496738640519096	No Hit
GTAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA	175	0.1488234443697965	No Hit
GTAATGGGGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAA	175	0.1488234443697965	No Hit
GTAATGGGGGTACTGTATGTGTTAATTTCTACTTTTTTAATATTTAAAATTG	174	0.14797302468768336	No Hit
GTAATGGGGTACAAGCCCCTAGACCTGCGACCCAAGAAGACTAGAGCCATGC	172	0.14627218532345712	No Hit
GTAATGGGGGCTTCACACTGACGCCGGAAGGGGATGAACCGCTTCCCGGTGC	171	0.145421765641344	No Hit
GTAATGGGGATGATCCGAAAGAACAAGTACCGCCCTGATCTGCGTATGGCGG	171	0.145421765641344	No Hit
GTAATGGGGAGGGCTCAGTCTTCTCTCCCAGCTCCAGCAGCTGTGGAAGAGG	168	0.14287050659500464	No Hit
GTAATGGGGGACAGTCACTGTAGTCCAGGATCACAGCCCATCATGGCCTAGA	168	0.14287050659500464	No Hit
GTAATGGGGAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAA	164	0.13946882786655215	No Hit
GTAATGGGGATTGTTACCCAGGGCAGGGGTACAGGGGAAGCTGCAGCTATTA	162	0.1377679885023259	No Hit
GTAATGGGGGTCAGACGTTACCGAGTCGCCCACGAGGTCCAGCCACATGGGC	162	0.1377679885023259	No Hit
GTAATGGGGGCTTTCCACCTCCCCAGATCCCTTTCTCTGGCCCAGTCTTTGG	161	0.13691756882021278	No Hit
GTAATGGGGATTTTAAAGGATTAAATCATGTGTCCTCTGATACATAAGCAAG	161	0.13691756882021278	No Hit
GTAATGGGGATTTGTGTATCAACATGTGACCTTGGTCCCCCCTCTACCTCCT	159	0.13521672945598653	No Hit
GTAATGGGGGTGAAGTGTACTTACTTTTAATAAAAAGGATATTGTTTGGAAG	159	0.13521672945598653	No Hit
GTAATGGGGACTTTTCATTCTTTCTGTTTATTCATATAACTCATTATTGTGC	158	0.13436630977387343	No Hit
GTAATGGGGATTCCGAGTGGAGCCTCTGTTCTGATTGATCTTCAGGGCCCTG	158	0.13436630977387343	No Hit
GTAATGGGGATTCTAAATCCAGTCACTTTAACAACGGAGATTAACCATCACA	158	0.13436630977387343	No Hit
GTAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	158	0.13436630977387343	No Hit
GTAATGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG	157	0.1335158900917603	No Hit
GTAATGGGGGTAGAAAATATTTTTTTCTCTGCCATTTATTTGGGAAAAAGTA	156	0.13266547040964716	No Hit
GTAATGGGGATCTGGCCTCTGCAGGATGGCTTTGCGGGCCTGTACTCCGCTG	153	0.13011421136330778	No Hit
GTAATGGGGATCTTGACCCATATCTGTACTATTGCAGATGGTCACTCTGAAG	151	0.12841337199908154	No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAA	151	0.12841337199908154	No Hit
GTAATGGGGTGGCTGGGATCAGAGGCTTTAAAATCTCTTTTCATCTTGGAGC	149	0.1267125326348553	No Hit
GTAATGGGGATGTGAGATGGCACTGGACTGAGAGACCTGGGAGCAAGCCCTG	147	0.12501169327062905	No Hit
GTAATGAGGATCCTTTGCTTCCTCTTTGGCAACCCCAGCCTCGCTCTGGCTC	147	0.12501169327062905	No Hit
GTAATGGGGAGTTTGCAAGAATCTCCCCGTTCCTTCTCTCTGATGCAGGCAC	147	0.12501169327062905	No Hit
GTAATGGGGGATCTCCCTAGCCCTATGGCTGGGCACAGTGGGCACACGTGGG	146	0.12416127358851592	No Hit
GTAATGGGGAGCAACACAGATACTTTGTGGAGGCCTATGCCAAAGTTGGCCC	145	0.12331085390640281	No Hit
GTAATGGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT	144	0.12246043422428968	No Hit
GTAATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	143	0.12161001454217657	No Hit
GTAATGGGGGGTAGGAGATAACCAGTCTTGTTTGTTTCTTACATGGCATTCC	142	0.12075959486006344	No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGCAGATCGGACAGACGCGCTTCT	142	0.12075959486006344	No Hit
GTAATGGGGAACTGAAAATCACGGAAAATGAGAAATACACACATTAGGATGT	140	0.11905875549583719	No Hit
GTAATGGGGATTGCTGGGATACCCAATGTACTGTTCCACTCCGGACATCTCA	140	0.11905875549583719	No Hit
GTAATGGGGACAGCTTACCTGGCTTGGAGTTCAGGTAATGGTGGCCATCCTG	139	0.11820833581372409	No Hit
GTAATGGGGTGACTGTTCTTTCCCAGATGAAGTCTAGTGCCAATTTTGTCTT	138	0.11735791613161095	No Hit
GTAATGTGGGGAAGGGGCGGGGCTTCCTCGGCAAGATGGCGGCCGCGGCCAC	137	0.11650749644949783	No Hit
GTAATGGGGAAATTAATCATTTTCAAACTCGTACATACGAGTTATTGCTTTA	135	0.11480665708527159	No Hit
GTAATGGGGGTGAATCTCTATTTGTCATTTTGTATTTGTATATTAAAATTAA	134	0.11395623740315845	No Hit
GTAATGGGGAACTCTGACTTCTTCAGCAACTCTCGGCTTGCCTCTCCTGCCC	133	0.11310581772104535	No Hit
GTAATGGGGAGGAGAAGTGGTAGGTCTAGGTCCAGAACACCAGCCAGGAGAA	132	0.11225539803893221	No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGGAG	132	0.11225539803893221	No Hit
GTAATGGGGGGTCTGGGTGCAGCCAGTGCAGCAGGATGCCGGAGCCTTCCCG	131	0.1114049783568191	No Hit
GTAATGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	131	0.1114049783568191	No Hit
GTAATGGGGGATACTGGGTCGCCGTCGCCAGCTCCTGGATGTTCATCTGGAT	131	0.1114049783568191	No Hit
GTAATGGGGCTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGG	130	0.11055455867470597	No Hit
GTAATGGGGGTTCCTCGCCGACACCAGCCAACTACTGTCTGCAGTGAAGGAG	129	0.10970413899259283	No Hit
GTAATGGGGAGATACTTTTTACACTTTTACAAAATGTTGCACAAGAGGAAGC	128	0.10885371931047973	No Hit
GTAATGGGGTATGAAACTCCTACTGGAGCTCAATTTGTAATTTAGAAACATT	128	0.10885371931047973	No Hit
GTAATGGGGGTTGATTTGTGTTCTGTGTCTGTTTTGGCTCAGTTTCCTTACC	128	0.10885371931047973	No Hit
GTAATGGGGGGTCATTAGGCGAGTAGACAGGAGAGTCCCCGCATTGACCAGC	128	0.10885371931047973	No Hit
GTAATGGGGGGATCAGTATAGTAAAAATGTCCATCTTGCTGAAAGCAATCTA	127	0.10800329962836659	No Hit
GTAATGGGGAGGGGTCCCACATTCTTGTGTAGTTTGCACAGGTACATGTGCA	127	0.10800329962836659	No Hit
GTAATGGGGTAATCACTGAGTTGTTTATAAAAGACACATCAAACTTTTCAAG	126	0.10715287994625347	No Hit
GTAATGGGGTGCTGCCGTTCGGTCTGGCGCACCTTCCGGCACGAAATACACC	125	0.10630246026414035	No Hit
GTAATGGGATTTATCATTGTAAAACCAAGGACAATTTTATAACTTTTTTGTA	125	0.10630246026414035	No Hit
GTAATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGAAGCA	125	0.10630246026414035	No Hit
GTAATGGGGTTGATAATATCGAAGAGTGTGAAGAAAGGCTTGCACAAGCCGA	123	0.1046016208999141	No Hit
GTAATGGGGACTAAAATCAGACTGCAAAATTCTTGTCCTGCAGGAATGGGAC	123	0.1046016208999141	No Hit
GTAATGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	122	0.10375120121780099	No Hit
GTAATGGGGCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGTCACGG	122	0.10375120121780099	No Hit
GTAATGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	122	0.10375120121780099	Illumina Single End Adapter 2 (97% over 34bp)
GTAATGGGGGACTGTCAGTCCTGGAGAATTAGAACATGCCTGCTAAATTTCA	122	0.10375120121780099	No Hit
GTAATGGGGGTTTTTCTTTCTCCAGCAGCCGAGCAAGATGCCCAAAGGGAAG	119	0.10119994217146161	No Hit
GTAATGGGGTGAACACTCAAGCTAACCAACAGCTCTTTAGCAGAAATTTAAG	119	0.10119994217146161	No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	119	0.10119994217146161	No Hit
GTAATGGGGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATAC	118	0.1003495224893485	No Hit
GTAATGGGGGATTACTAAATCTTCAGTTTTTTTAAGCTTGTATTTTTGTACA	118	0.1003495224893485	No Hit
GTAATGGAGAGAGCTGGAATGTAACGTTGCTTACAAGCATTGCTATTCAACT	118	0.1003495224893485	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTC	118	0.1003495224893485	No Hit
GTAATGGGGATCTGTGGTTTTACATTTCTTTTATGGTTCTCAAGCTGGTGCA	118	0.1003495224893485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.007653777139018106	0.0	0.0	0.0	0.0
8	0.014457134595923089	0.0	0.0	0.0	0.0
9	0.052726020291013614	0.0	0.0	0.0	0.0
10	0.2126049205282807	0.0	0.0	0.0	0.0
11	0.3240098988850998	0.0	0.0	0.0	0.0
12	0.38949221440781023	0.0	0.0	0.0	0.0
13	0.42265858201022205	0.0	0.0	0.0	0.0
14	0.5425677571881724	0.0	0.0	0.0	0.0
15	0.6131525908035615	0.0	0.0	0.0	0.0
16	0.678634906326272	0.0	0.0	0.0	0.0
17	0.7211558904319282	0.0	0.0	0.0	0.0
18	0.7585743564449056	0.0	0.0	0.0	0.0
19	0.8010953405505618	0.0	0.0	0.0	0.0
20	0.865727236391159	0.0	0.0	0.0	0.0
21	0.9150515779537202	0.0	0.0	0.0	0.0
22	0.9592734014236025	0.0	0.0	0.0	0.0
23	1.0400632712243492	0.0	0.0	0.0	0.0
24	1.114900203250304	0.0	0.0	0.0	0.0
25	1.1854850368656933	0.0	0.0	0.0	0.0
26	1.2475656736599512	0.0	0.0	0.0	0.0
27	1.3164496679111142	0.0	0.0	0.0	0.0
28	1.4899352830621913	0.0	0.0	0.0	0.0
29	1.5256529097109424	0.0	0.0	0.0	0.0
30	1.598789002372671	0.0	0.0	0.0	0.0
31	1.6370578880677615	0.0	0.0	0.0	0.0
32	1.667672996623834	0.0	0.0	0.0	0.0
33	1.7008393642262456	0.0	0.0	0.0	0.0
34	1.7365569908749967	0.0	0.0	0.0	0.0
35	1.924499740621997	0.0	0.0	0.0	0.0
36	1.9568156885422956	0.0	0.0	0.0	0.0
37	1.9899820561447075	0.0	0.0	0.0	0.0
38	2.0205971647007797	0.0	0.0	0.0	0.0
39	2.0537635323031918	0.0	0.0	0.0	0.0
40	2.091181998316169	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTTGG	35	1.00912075E-7	46.000004	38
ACTGCTC	35	1.00912075E-7	46.000004	46
CGTTCCC	35	1.00912075E-7	46.000004	15
TTATGCT	35	1.00912075E-7	46.000004	36
GGATGAC	35	1.00912075E-7	46.000004	8
TGGCAAC	35	1.00912075E-7	46.000004	27
GTGTCAG	35	1.00912075E-7	46.000004	33
TCAAGGG	35	1.00912075E-7	46.000004	29
TAAACGA	35	1.00912075E-7	46.000004	32
CACACCC	35	1.00912075E-7	46.000004	25
GCCCAAC	70	0.0	46.000004	15
TCTAGCA	35	1.00912075E-7	46.000004	39
GAAACCC	70	0.0	46.000004	46
TGCGTGG	35	1.00912075E-7	46.000004	42
TGCGCAG	35	1.00912075E-7	46.000004	31
TTACCCG	35	1.00912075E-7	46.000004	38
AATTCGG	35	1.00912075E-7	46.000004	38
TGTCTCA	35	1.00912075E-7	46.000004	13
CGGGGCT	35	1.00912075E-7	46.000004	18
CCCGGCG	35	1.00912075E-7	46.000004	29
>>END_MODULE