FastQCFastQC Report
Fri 17 Jun 2016
SRR1527312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4274299
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC655471.5335146184204709No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG343890.8045529805004283No Hit
CCTAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA341090.7980021987231122No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC328190.7678218112490492No Hit
CCTAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG277560.6493696393256532No Hit
CCTAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG186820.4370775184422053No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT173680.40633563538722955No Hit
CCTAGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG169050.3955034498054535No Hit
CCTAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC151750.3550289766813225No Hit
CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT151070.3534380725354029No Hit
CCTAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC131050.3065999828275935No Hit
CCTAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC129280.3024589529183616No Hit
CCTAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT122230.2859650202290481No Hit
CCTAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC121150.2834382901149405No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT118300.276770530091601No Hit
CCTAGAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA112510.26322444920207966No Hit
CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC104610.2447418863303667No Hit
CCTAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG102010.2386590175371447No Hit
CCTAGAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAA99520.2328335008851744No Hit
CCTAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG83060.1943242622942382No Hit
CCTAGAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC81500.190674541018305No Hit
CCTAGAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA79570.1861591807217979No Hit
CCTAGAGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCAT79370.1856912677377039No Hit
CCTAGAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT77590.1815268421792673No Hit
CCTAGAGGGGCTCCGCAGCCTGCTGTCCGCCTGCCTTTTCGTCTCTAGCC75050.17558434728127348No Hit
CCTAGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA74520.17434437787342438No Hit
CCTAGAGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT71550.1673958700596285No Hit
CCTAGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA66930.1565870801270571No Hit
CCTAGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA66550.1556980454572785No Hit
CCTAGAGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCA63770.1491940549783719No Hit
CCTAGAGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCC61350.1435323078708345No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG60800.142245547164576No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG60690.1419881950233243No Hit
CCTAGAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGC58800.137566417323636No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC58340.1364902174602198No Hit
CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT56060.1311560094415482No Hit
CCTAGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCAT55730.1303839530177931No Hit
CCTAGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC55650.1301967878241555No Hit
CCTAGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC54410.1272957273227727No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC53490.1251433275959403No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC53400.12493276675309799No Hit
CCTAGAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC52140.12198491495330578No Hit
CCTAGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC50930.1191540413995371No Hit
CCTAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC48070.1124628857269929No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC47950.11218213793653649No Hit
CCTAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC45690.10689472121627429No Hit
CCTAGAGGGGCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC44790.10478911278785129No Hit
CCTAGAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCC44620.10439138675137138No Hit
CCTAGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC43670.1021688000769249No Hit
CCTAGAGGGGACTCTGCAGACTCAGTGCCTTACTCAGACTCCTCTCTCGC42830.10020356554373008No Hit
CCTAGAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA42820.1001801698945254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA207.858887E-444.042
TAACGGG950.044.042
TACGCTA408.3218765E-944.013
ACGATAA408.3218765E-944.044
CGTTTAT254.4448785E-544.042
ACGAATA254.4448785E-544.031
CTAGAGG4339650.043.1189162
CCTAGAG4354300.043.070851
TAGAGGG4329850.043.058493
AGAGGGG3311100.042.9860764
GAGGGGG1545250.042.6958735
GAGGGGA942850.042.6886565
AGGGATC68250.042.484986
AGAGGGA755050.042.452824
AGGGGGC431950.042.451676
GAGGGAT219550.042.366665
AGGGGGG674700.042.343566
AGGGGAG482750.042.3047146
AGGGGGT260250.042.292416
GAGGGGT318600.042.1424945
Produced by FastQC (version 0.11.5)