##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527312_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4274299 Sequences flagged as poor quality 0 Sequence length 50 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53421835954855 33.0 31.0 34.0 31.0 34.0 2 33.07399880073902 34.0 31.0 34.0 31.0 34.0 3 32.99176496543644 34.0 31.0 34.0 31.0 34.0 4 36.3933634497727 37.0 37.0 37.0 35.0 37.0 5 36.35504067450592 37.0 37.0 37.0 35.0 37.0 6 36.06055940400987 37.0 37.0 37.0 35.0 37.0 7 36.529879636403535 37.0 37.0 37.0 35.0 37.0 8 36.68551544943393 37.0 37.0 37.0 35.0 37.0 9 38.77777315063827 39.0 39.0 39.0 39.0 39.0 10 38.35913748663816 39.0 39.0 39.0 37.0 39.0 11 38.01468310944087 39.0 38.0 39.0 35.0 39.0 12 37.224897462718445 39.0 35.0 39.0 35.0 39.0 13 37.04318345534554 39.0 35.0 39.0 34.0 39.0 14 37.64787489129797 40.0 37.0 40.0 33.0 40.0 15 37.74123967462267 40.0 37.0 40.0 33.0 40.0 16 37.67767322782051 40.0 36.0 40.0 33.0 40.0 17 37.61227653938108 40.0 36.0 40.0 33.0 40.0 18 37.617560680710454 40.0 36.0 40.0 33.0 40.0 19 37.61529761956288 40.0 36.0 40.0 33.0 40.0 20 37.354840875661715 40.0 36.0 40.0 33.0 40.0 21 37.532434207340195 40.0 36.0 40.0 33.0 40.0 22 37.41939344907785 40.0 36.0 40.0 33.0 40.0 23 37.47169769826584 40.0 35.0 40.0 33.0 40.0 24 37.50549341541151 40.0 35.0 40.0 33.0 40.0 25 37.45190076782181 40.0 35.0 40.0 33.0 40.0 26 37.299602812063455 40.0 35.0 40.0 33.0 40.0 27 37.182955146563216 39.0 35.0 40.0 33.0 40.0 28 36.98029454654436 39.0 35.0 40.0 32.0 40.0 29 37.21336995844231 40.0 35.0 40.0 33.0 40.0 30 37.2204773227142 40.0 35.0 40.0 33.0 40.0 31 37.225278577843994 40.0 35.0 40.0 33.0 40.0 32 36.56933593087428 40.0 35.0 40.0 31.0 40.0 33 36.74754059086648 39.0 35.0 40.0 32.0 40.0 34 36.530212088578736 39.0 35.0 40.0 31.0 40.0 35 36.53714024217772 39.0 35.0 40.0 31.0 40.0 36 36.40701644877909 39.0 35.0 40.0 31.0 40.0 37 36.31574627792768 39.0 35.0 40.0 31.0 40.0 38 36.10678967475135 38.0 35.0 40.0 31.0 40.0 39 36.02214070658136 38.0 35.0 40.0 30.0 40.0 40 35.833252891292815 38.0 35.0 40.0 30.0 40.0 41 35.616417335333814 37.0 35.0 40.0 30.0 40.0 42 35.556925708753646 37.0 35.0 40.0 30.0 40.0 43 35.45342546228048 37.0 35.0 40.0 29.0 40.0 44 35.2905760687308 36.0 35.0 40.0 29.0 40.0 45 35.14527317812816 36.0 34.0 40.0 29.0 40.0 46 34.83547805148868 35.0 34.0 40.0 28.0 40.0 47 34.68595224620458 35.0 34.0 40.0 27.0 40.0 48 34.54968990236762 35.0 34.0 39.0 27.0 40.0 49 34.256370693767565 35.0 34.0 39.0 27.0 40.0 50 33.9430919549615 35.0 33.0 39.0 26.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 9.0 15 55.0 16 106.0 17 315.0 18 779.0 19 1536.0 20 2717.0 21 4210.0 22 6845.0 23 10086.0 24 13966.0 25 18984.0 26 25241.0 27 32658.0 28 43523.0 29 56829.0 30 77697.0 31 99766.0 32 121269.0 33 165993.0 34 348186.0 35 394846.0 36 390808.0 37 695435.0 38 1165525.0 39 596914.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.71447949710584 0.24427397334627268 0.020237236562065497 0.0210092929858206 8 99.77937902799968 0.18295397678075398 0.018646332416145897 0.019020662803421098 9 99.54858094859532 0.302973657200865 0.07769695100880869 0.0707484431950128 10 76.16009081255196 17.282155506669046 2.1302206513863444 4.427533029392656 11 43.14246616813658 26.155984876116527 14.141617140026938 16.55993181571996 12 35.055970581374865 13.05622278647329 24.70989979877402 27.177906833377826 13 25.818736592830778 14.775335090034647 29.29453461257624 30.11139370455834 14 24.136191688976368 14.19285361178523 33.911034300595254 27.759920398643146 15 26.01666378510254 15.120537894049995 31.223388911257725 27.63940940958974 16 32.698250637122015 15.587023743542508 24.956957854375652 26.757767764959823 17 33.603919613485154 15.443585018268493 24.888876515189974 26.063618853056376 18 32.12498704465926 17.88826659061521 22.91992207377163 27.066824290953907 19 32.33936137832192 18.85794606320241 20.79414659573418 28.008545962741493 20 30.362499207472382 21.189182132555537 22.374546095160866 26.073772564811215 21 31.53754568877844 18.64406303817304 24.092675781455625 25.725715491592894 22 30.228956841811954 16.681331839443146 26.06530333979911 27.024407978945785 23 30.217001665068356 17.648999286198745 24.08600802143229 28.04799102730062 24 31.816141079508007 17.2351302517676 22.553639789822846 28.395088878901547 25 30.501445968099095 17.113309106358727 21.782565983334344 30.602678942207834 26 27.6870195557213 16.660065194316072 24.13579396293989 31.517121287022736 27 27.480997468824715 15.636482145961244 24.00931708333928 32.87320330187477 28 29.146744296550146 19.14433220511714 21.054610358330102 30.654313140002603 29 31.492041151075302 19.84077389064265 21.25108702035117 27.41609793793087 30 30.4795710360927 16.86737404191892 24.301037433272686 28.352017488715692 31 33.70578427012242 16.970829602702104 21.49547797194347 27.827908155232006 32 36.09036241966226 17.93213343287402 20.02442973689955 25.953074410564163 33 36.665053146726514 16.47238529639597 21.79824106830149 25.06432048857602 34 30.321977943049845 18.696188544601114 27.553383607464056 23.42844990488499 35 28.78137444292035 24.033508184616938 24.852894006713147 22.332223365749567 36 32.47126604853802 23.654030754516704 21.329626214731352 22.545076982213928 37 31.885555970698356 23.37688589403783 19.280541674786907 25.45701646047691 38 33.34004008610535 22.992284816761767 19.227082616354167 24.44059248077872 39 33.83726781865284 20.05566292858782 19.266457493965678 26.840611758793663 40 32.060227887660645 18.777652195131882 19.20225983254798 29.959860084659496 41 27.69740722396819 21.512837543653358 21.12049250649054 29.66926272588792 42 26.605368506040406 18.630680726827954 22.261077196518073 32.502873570613566 43 26.18899613714436 16.087760823470703 26.111462955679983 31.611780083704954 44 26.474399661792496 16.4282143106975 24.814712307211074 32.28267372029893 45 30.451777004837517 17.183191910533164 23.390759514016217 28.974271570613098 46 29.285597474580044 20.555160039108166 21.226895919073513 28.932346567238277 47 26.322936228841264 19.680232945800004 24.37803251480535 29.61879831055338 48 28.590723297551246 19.568495325198356 26.076463064469753 25.76431831278064 49 30.82393159673668 18.6946210361044 23.80364593118076 26.677801435978154 50 27.130928369774786 18.651479458970933 22.836867519094945 31.380724652159337 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 9.5 10 19.0 11 124.0 12 229.0 13 1012.0 14 1795.0 15 2774.5 16 3754.0 17 3699.5 18 3645.0 19 3935.0 20 4225.0 21 5059.5 22 5894.0 23 5357.0 24 4820.0 25 5241.5 26 5663.0 27 7517.5 28 9372.0 29 10125.0 30 10878.0 31 13948.5 32 17019.0 33 17536.0 34 18053.0 35 20807.5 36 23562.0 37 27265.0 38 30968.0 39 32312.5 40 33657.0 41 39809.0 42 45961.0 43 49925.0 44 53889.0 45 58981.0 46 64073.0 47 78204.0 48 92335.0 49 102672.0 50 113009.0 51 137151.0 52 161293.0 53 171260.0 54 181227.0 55 217496.0 56 253765.0 57 310438.0 58 367111.0 59 425627.0 60 484143.0 61 522586.0 62 561029.0 63 559098.0 64 557167.0 65 476672.0 66 396177.0 67 325854.0 68 255531.0 69 215728.0 70 175925.0 71 152456.5 72 128988.0 73 106470.5 74 83953.0 75 77722.0 76 71491.0 77 53607.0 78 35723.0 79 23212.0 80 10701.0 81 7848.0 82 4995.0 83 3195.5 84 1396.0 85 893.5 86 391.0 87 303.0 88 215.0 89 137.5 90 60.0 91 57.0 92 54.0 93 99.0 94 144.0 95 72.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4274299.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 5.930117474446424 #Duplication Level Percentage of deduplicated Percentage of total 1 65.01715312028374 3.8555935585735344 2 10.858745540753889 1.2878727336358364 3 4.980747964555469 0.8860926162127155 4 2.746571858324754 0.6514997508749764 5 1.5430949887597707 0.45753672787875116 6 1.0225738199371297 0.3638389727112362 7 0.6829935271117078 0.28351622950412547 8 0.4926057780990114 0.23369681061745795 9 0.3942838334226849 0.21043345054244283 >10 5.009619257170607 7.966892242697994 >50 3.658384700079773 15.792341136670728 >100 3.282689758699811 35.46296364704322 >500 0.18929077879939546 7.665345185196661 >1k 0.1035119509338742 11.748493040484211 >5k 0.010309955272298228 4.037073319561474 >10k+ 0.007423167796054724 9.09681057779461 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 65547 1.5335146184204709 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 34389 0.8045529805004283 No Hit CCTAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 34109 0.7980021987231122 No Hit CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 32819 0.7678218112490492 No Hit CCTAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG 27756 0.6493696393256532 No Hit CCTAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 18682 0.4370775184422053 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT 17368 0.40633563538722955 No Hit CCTAGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG 16905 0.3955034498054535 No Hit CCTAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 15175 0.3550289766813225 No Hit CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 15107 0.3534380725354029 No Hit CCTAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC 13105 0.3065999828275935 No Hit CCTAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC 12928 0.3024589529183616 No Hit CCTAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT 12223 0.2859650202290481 No Hit CCTAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC 12115 0.2834382901149405 No Hit CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 11830 0.276770530091601 No Hit CCTAGAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA 11251 0.26322444920207966 No Hit CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC 10461 0.2447418863303667 No Hit CCTAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 10201 0.2386590175371447 No Hit CCTAGAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAA 9952 0.2328335008851744 No Hit CCTAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG 8306 0.1943242622942382 No Hit CCTAGAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC 8150 0.190674541018305 No Hit CCTAGAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA 7957 0.1861591807217979 No Hit CCTAGAGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCAT 7937 0.1856912677377039 No Hit CCTAGAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT 7759 0.1815268421792673 No Hit CCTAGAGGGGCTCCGCAGCCTGCTGTCCGCCTGCCTTTTCGTCTCTAGCC 7505 0.17558434728127348 No Hit CCTAGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA 7452 0.17434437787342438 No Hit CCTAGAGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT 7155 0.1673958700596285 No Hit CCTAGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA 6693 0.1565870801270571 No Hit CCTAGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA 6655 0.1556980454572785 No Hit CCTAGAGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCA 6377 0.1491940549783719 No Hit CCTAGAGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCC 6135 0.1435323078708345 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG 6080 0.142245547164576 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG 6069 0.1419881950233243 No Hit CCTAGAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGC 5880 0.137566417323636 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC 5834 0.1364902174602198 No Hit CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 5606 0.1311560094415482 No Hit CCTAGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCAT 5573 0.1303839530177931 No Hit CCTAGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC 5565 0.1301967878241555 No Hit CCTAGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC 5441 0.1272957273227727 No Hit CCTAGAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC 5349 0.1251433275959403 No Hit CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC 5340 0.12493276675309799 No Hit CCTAGAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC 5214 0.12198491495330578 No Hit CCTAGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC 5093 0.1191540413995371 No Hit CCTAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 4807 0.1124628857269929 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC 4795 0.11218213793653649 No Hit CCTAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC 4569 0.10689472121627429 No Hit CCTAGAGGGGCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC 4479 0.10478911278785129 No Hit CCTAGAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCC 4462 0.10439138675137138 No Hit CCTAGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC 4367 0.1021688000769249 No Hit CCTAGAGGGGACTCTGCAGACTCAGTGCCTTACTCAGACTCCTCTCTCGC 4283 0.10020356554373008 No Hit CCTAGAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA 4282 0.1001801698945254 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.005919099248789099 0.0 0.0 0.0 0.0 5 0.005919099248789099 0.0 0.0 0.0 0.0 6 0.007603585991527499 0.0 0.0 0.0 0.0 7 0.007603585991527499 0.0 0.0 0.0 0.0 8 0.0076503772899369 0.0 0.0 0.0 0.0 9 0.0078141468343698 0.0 0.0 0.0 0.0 10 0.010364272597682099 0.0 0.0 0.0 1.4037389522819998E-4 11 0.0158154588623772 0.0 0.0 0.0 1.4037389522819998E-4 12 0.0194417844891057 0.0 0.0 0.0 1.4037389522819998E-4 13 0.0232084840110624 0.0 0.0 0.0 1.4037389522819998E-4 14 0.0286128789773481 0.0 0.0 0.0 1.4037389522819998E-4 15 0.0334791740119257 0.0 0.0 0.0 1.4037389522819998E-4 16 0.0402873079304934 0.0 0.0 0.0 1.4037389522819998E-4 17 0.044896250823819296 0.0 0.0 0.0 1.4037389522819998E-4 18 0.0487799285917995 0.0 0.0 0.0 1.4037389522819998E-4 19 0.055260523421501395 0.0 0.0 0.0 1.4037389522819998E-4 20 0.059799279367213194 0.0 0.0 0.0 1.4037389522819998E-4 21 0.0636595614859887 0.0 0.0 0.0 1.4037389522819998E-4 22 0.06656062198737149 0.0 0.0 0.0 1.4037389522819998E-4 23 0.0699763867712577 0.0 0.0 0.0 1.4037389522819998E-4 24 0.0736495036963956 0.0 0.0 0.0 1.4037389522819998E-4 25 0.07713545542789589 0.0 0.0 0.0 1.4037389522819998E-4 26 0.081182902740309 0.0 0.0 0.0 1.4037389522819998E-4 27 0.0852537457019268 0.0 0.0 0.0 1.4037389522819998E-4 28 0.0897223147000245 0.0 0.0 0.0 1.4037389522819998E-4 29 0.09348901422198119 0.0 0.0 0.0 1.4037389522819998E-4 30 0.0975130658851896 0.0 0.0 0.0 1.4037389522819998E-4 31 0.1019114479356732 0.0 0.0 0.0 1.4037389522819998E-4 32 0.10841543841457979 0.0 0.0 0.0 1.4037389522819998E-4 33 0.11265005092063049 0.0 0.0 0.0 1.4037389522819998E-4 34 0.11805444588691619 0.0 0.0 0.0 1.4037389522819998E-4 35 0.1260323622657189 0.0 0.0 0.0 1.4037389522819998E-4 36 0.1369815260935185 0.0 0.0 0.0 1.4037389522819998E-4 37 0.1443277599437943 0.0 0.0 0.0 1.4037389522819998E-4 38 0.15094872866872439 0.0 0.0 0.0 1.4037389522819998E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATGA 20 7.858887E-4 44.0 42 TAACGGG 95 0.0 44.0 42 TACGCTA 40 8.3218765E-9 44.0 13 ACGATAA 40 8.3218765E-9 44.0 44 CGTTTAT 25 4.4448785E-5 44.0 42 ACGAATA 25 4.4448785E-5 44.0 31 CTAGAGG 433965 0.0 43.118916 2 CCTAGAG 435430 0.0 43.07085 1 TAGAGGG 432985 0.0 43.05849 3 AGAGGGG 331110 0.0 42.986076 4 GAGGGGG 154525 0.0 42.695873 5 GAGGGGA 94285 0.0 42.688656 5 AGGGATC 6825 0.0 42.48498 6 AGAGGGA 75505 0.0 42.45282 4 AGGGGGC 43195 0.0 42.45167 6 GAGGGAT 21955 0.0 42.36666 5 AGGGGGG 67470 0.0 42.34356 6 AGGGGAG 48275 0.0 42.304714 6 AGGGGGT 26025 0.0 42.29241 6 GAGGGGT 31860 0.0 42.142494 5 >>END_MODULE