FastQCFastQC Report
Fri 17 Jun 2016
SRR1527311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4648861
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC636881.3699699775923608No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA332980.716261467056124No Hit
CCTAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC330320.7105396354074687No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT329560.7089048263649956No Hit
CCTAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG266720.5737319313268346No Hit
CCTAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA175820.3782001655889475No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC173540.3732957384615285No Hit
CCTAGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG159300.34266457956045576No Hit
CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG143500.30867775999325425No Hit
CCTAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT139110.2992345867084432No Hit
CCTAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC133490.2871456040522614No Hit
CCTAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA130600.28092902756180493No Hit
CCTAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG118740.2554174022411081No Hit
CCTAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG115820.24913629381476451No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC110930.2386175882651686No Hit
CCTAGAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC107990.23229345854823363No Hit
CCTAGAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG107660.23158360725347563No Hit
CCTAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC96080.20667428000105834No Hit
CCTAGAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC86760.18662635858546855No Hit
CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT82670.17782850465952843No Hit
CCTAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG81300.17488154625401792No Hit
CCTAGAGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC77880.1675249055628895No Hit
CCTAGAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCAC77070.16578254329393802No Hit
CCTAGAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA76630.16483607490092733No Hit
CCTAGAGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT74260.15973805196584712No Hit
CCTAGAGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCATAC73990.1591572645428633No Hit
CCTAGAGGGGCTCCGCAGCCTGCTGTCCGCCTGCCTTTTCGTCTCTAGCCGC70510.15167155997996068No Hit
CCTAGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA68970.14835892060442332No Hit
CCTAGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA66880.14386319573762263No Hit
CCTAGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG65690.14130342894743464No Hit
CCTAGAGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCAGT62440.13431246922633305No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT59680.12837553112472067No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT59190.1273215095052315No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC57600.12390131690321565No Hit
CCTAGAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCGG56650.12185780560012441No Hit
CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT54790.11785682557512475No Hit
CCTAGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT54480.11718999557095812No Hit
CCTAGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT53470.11501742039609272No Hit
CCTAGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG53190.1144151223278132No Hit
CCTAGAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG50880.10944616326450717No Hit
CCTAGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG49150.10572482162835153No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC48320.10393943806880869No Hit
CCTAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC47480.10213254386397012No Hit
CCTAGAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG47070.10125060740684654No Hit
CCTAGAGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTT47040.10118607547095944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC1650.046.00000411
AACGTAT206.313448E-446.042
CTAGAGG4721450.045.0617682
CCTAGAG4738600.045.003041
TAGAGGG4710750.044.9800573
AGAGGGG3615400.044.8816154
AGGGATC73100.044.6785246
GAGGGGG1678400.044.5350955
AGGGGGG740150.044.499096
GAGGGGA1040250.044.487675
AGAGGGA805800.044.421574
GAGGGAT235450.044.3784265
AGGGGAG532550.044.285426
GAGGGGT339050.044.1005745
AGGGGGC452750.043.978136
AGGGGGT286800.043.9149256
GAGGGGC606700.043.3273475
AGGGGAC243500.043.128546
GAGGGAC158150.043.0331955
AGGGGAT154600.042.8311776
Produced by FastQC (version 0.11.5)