##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527311_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4648861 Sequences flagged as poor quality 0 Sequence length 52 %GC 59 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.489121313801384 33.0 31.0 34.0 31.0 34.0 2 33.06283108916356 34.0 31.0 34.0 31.0 34.0 3 32.983516392509905 34.0 31.0 34.0 31.0 34.0 4 36.33464433546195 37.0 37.0 37.0 35.0 37.0 5 36.35547976160182 37.0 37.0 37.0 35.0 37.0 6 36.04870160669463 37.0 36.0 37.0 35.0 37.0 7 36.55487225795738 37.0 37.0 37.0 35.0 37.0 8 36.662430646990735 37.0 37.0 37.0 35.0 37.0 9 38.74976537263644 39.0 39.0 39.0 39.0 39.0 10 38.268743247001794 39.0 39.0 39.0 37.0 39.0 11 37.918626519485095 39.0 38.0 39.0 35.0 39.0 12 37.15141278691706 39.0 35.0 39.0 34.0 39.0 13 37.014770714805195 39.0 35.0 39.0 34.0 39.0 14 37.65905605695675 40.0 37.0 40.0 33.0 40.0 15 37.651925708254126 40.0 36.0 40.0 33.0 40.0 16 32.66851536322553 39.0 34.0 40.0 3.0 40.0 17 34.223222849639946 39.0 33.0 40.0 17.0 40.0 18 36.2825834112915 39.0 34.0 40.0 30.0 40.0 19 37.21991021026441 39.0 35.0 40.0 32.0 40.0 20 37.46398741541208 39.0 36.0 40.0 33.0 40.0 21 37.66405556113637 40.0 36.0 40.0 33.0 40.0 22 37.75212509042537 40.0 36.0 40.0 34.0 40.0 23 37.72836916397371 40.0 36.0 40.0 34.0 40.0 24 37.705055926602235 40.0 36.0 40.0 34.0 40.0 25 37.656648800641705 40.0 36.0 40.0 34.0 40.0 26 37.55779512444016 40.0 35.0 40.0 34.0 40.0 27 37.44463299720082 40.0 35.0 40.0 33.0 40.0 28 37.29067485562592 40.0 35.0 40.0 33.0 40.0 29 37.08389861516617 39.0 35.0 40.0 33.0 40.0 30 37.00895230035916 39.0 35.0 40.0 32.0 40.0 31 36.99755380941697 39.0 35.0 40.0 32.0 40.0 32 36.958532638424764 39.0 35.0 40.0 32.0 40.0 33 36.618248641979186 39.0 35.0 40.0 32.0 40.0 34 36.28115682529549 39.0 35.0 40.0 30.0 40.0 35 36.22676565292014 38.0 35.0 40.0 30.0 40.0 36 36.24972417975069 38.0 35.0 40.0 31.0 40.0 37 35.96044708585608 38.0 35.0 40.0 30.0 40.0 38 35.93970673676843 38.0 35.0 40.0 30.0 40.0 39 35.54721661929664 37.0 34.0 40.0 29.0 40.0 40 35.4122923873181 37.0 34.0 40.0 29.0 40.0 41 35.24622267690946 37.0 34.0 40.0 29.0 40.0 42 35.261882211578275 36.0 34.0 40.0 29.0 40.0 43 35.160604715864814 36.0 34.0 40.0 29.0 40.0 44 35.44244837606459 36.0 34.0 40.0 30.0 40.0 45 35.49248278234174 36.0 35.0 40.0 30.0 40.0 46 35.39646808110632 36.0 35.0 40.0 30.0 40.0 47 35.0727524440933 35.0 34.0 40.0 30.0 40.0 48 34.994154051927985 35.0 34.0 39.0 30.0 40.0 49 34.91046452023409 35.0 34.0 39.0 30.0 40.0 50 34.44941976109847 35.0 34.0 39.0 28.0 40.0 51 34.49779290023943 35.0 34.0 39.0 29.0 40.0 52 34.14514157338755 35.0 34.0 38.0 28.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 8.0 15 45.0 16 104.0 17 336.0 18 721.0 19 1298.0 20 2450.0 21 4213.0 22 6482.0 23 10050.0 24 14505.0 25 20282.0 26 26974.0 27 36078.0 28 46762.0 29 61652.0 30 81800.0 31 110949.0 32 157679.0 33 252911.0 34 399062.0 35 466874.0 36 564250.0 37 883352.0 38 1042779.0 39 457242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.72974025250487 0.23736997083801817 0.018090452693681314 0.014799323963439647 8 99.7540687923343 0.18972389150804897 0.03839650185281943 0.01781081430483725 9 99.55541798302853 0.29630913894822836 0.07391057723601545 0.07436230078722508 10 76.4219020529975 17.05899574110734 2.0888557433745603 4.430246462520604 11 43.3173631132443 26.069912608701358 13.95860190270262 16.654122375351726 12 35.16736680231997 13.014004935832672 24.737779856184126 27.08084840566324 13 26.039969790449746 14.665570770991001 29.169123361614812 30.125336076944436 14 24.32952931911709 14.126750616979084 33.83331960237142 27.7104004615324 15 26.106287109896382 15.149302162400641 31.175399737699188 27.56901099000379 16 30.663618464823966 24.257576210602984 22.08291450314389 22.995890821429164 17 33.748008383128685 15.32472147478705 24.89383958780441 26.033430554279857 18 32.201027305398036 17.7635769277679 22.963065576707926 27.07233019012614 19 32.43723570139008 18.829709040558537 20.74376928026026 27.989285977791116 20 30.395208632824257 21.154472030891007 22.35726127324521 26.093058063039525 21 31.656162660058023 18.65172996138194 24.03479045727545 25.65731692128459 22 30.04357411417549 16.65132599146329 26.192587818822716 27.112512075538504 23 30.269134740746175 17.559333350685254 24.089061815356494 28.08247009321208 24 31.957290183552487 17.14957706844752 22.535649054682427 28.35748369331757 25 30.509129010310264 16.99504459264323 21.79426315392093 30.701563243125573 26 27.76312305315216 16.59931325113829 24.0805220891741 31.557041606535453 27 27.60104034084908 15.559165997864854 23.970129457516585 32.86966420376948 28 29.128382199424763 19.187861284731895 21.00727468513255 30.676481830710788 29 31.704518590682753 19.767358068997975 21.18985704240243 27.338266297916846 30 30.56950939165529 16.83857615876233 24.185451016926514 28.40646343265587 31 33.88950540788378 16.920144525723614 21.5019549949977 27.688395071394904 32 36.30908732267969 17.431452564402335 20.057579695327522 26.201880417590463 33 36.918440882616196 16.436456155604567 21.75122035268424 24.893882609095 34 30.46143990968971 18.60857960691877 27.5059632886421 23.424017194749425 35 28.863112921638223 24.02973545563096 24.836685803253744 22.270465819477074 36 32.436418296868844 23.620775067269165 21.392100129472574 22.550706506389414 37 32.054453768353156 23.387126438067302 19.228451872404875 25.329967921174674 38 33.308201729412865 23.054249202116388 19.131503394057166 24.50604567441358 39 34.01327766091522 20.1521620026927 19.22167601913673 26.612884317255347 40 32.11655500132183 18.74781801391782 19.138515864423567 29.99711112033679 41 27.753894986320304 21.4889625652391 21.094693947614264 29.662448500826333 42 26.607979890127925 18.629444932855595 22.20694488391888 32.5556302930976 43 26.24595572980134 15.99897265158068 26.095574808539123 31.659496810078856 44 26.384269179052676 16.36220140804382 24.850388084307102 32.403141328596405 45 30.553139790585266 17.076763534121582 23.31590469149325 29.054191983799903 46 29.059849283512673 20.51687499368125 21.326363597448925 29.096912125357154 47 26.544093273599707 19.702223835042602 24.350566730216283 29.40311616114141 48 28.68253105438085 19.564985918056056 26.01656620836803 25.735916819195065 49 30.80767525636925 18.5679459979552 23.889722665401266 26.734656080274288 50 27.145294298969148 18.837366830283806 22.78614051915082 31.23119835159623 51 25.705156596422217 21.519593724140172 24.284507538513196 28.490742140924414 52 27.15082253481014 20.076917765448353 22.780805879117487 29.99145382062402 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 10.0 10 18.0 11 121.0 12 224.0 13 1038.5 14 2875.5 15 3898.0 16 3925.5 17 3953.0 18 4195.0 19 4437.0 20 5141.0 21 5845.0 22 5431.0 23 5017.0 24 5219.5 25 5422.0 26 6620.0 27 7818.0 28 9478.0 29 11138.0 30 12072.0 31 13006.0 32 15263.5 33 17521.0 34 19451.0 35 21381.0 36 21989.0 37 22597.0 38 28492.5 39 36609.5 40 38831.0 41 42987.5 42 47144.0 43 50967.5 44 54791.0 45 66025.5 46 77260.0 47 84890.5 48 92521.0 49 107016.5 50 121512.0 51 138848.0 52 156184.0 53 190027.0 54 223870.0 55 240668.0 56 257466.0 57 325535.5 58 393605.0 59 453105.0 60 512605.0 61 534972.5 62 557340.0 63 578027.0 64 509890.0 65 421066.0 66 371000.5 67 320935.0 68 265689.0 69 210443.0 70 178379.5 71 146316.0 72 126617.5 73 106919.0 74 94306.5 75 81694.0 76 62934.5 77 44175.0 78 30991.0 79 17807.0 80 12117.5 81 6428.0 82 4221.5 83 2015.0 84 1235.0 85 455.0 86 257.5 87 60.0 88 47.0 89 34.0 90 34.0 91 40.0 92 46.0 93 59.5 94 73.0 95 36.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4648861.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.919485405494051 #Duplication Level Percentage of deduplicated Percentage of total 1 67.70352618994498 4.684735613718085 2 10.717882199435728 1.483244589136 3 4.66941867485279 0.9692992311835574 4 2.510471745733709 0.6948469042203829 5 1.448866960901544 0.5012706895230378 6 0.893306606005731 0.3708733213732848 7 0.5934891647962072 0.2874647729888346 8 0.4390663291613849 0.24304904453408396 9 0.3317633184673197 0.20660682961916046 >10 4.433650995866626 8.320904879555384 >50 3.2827795278719907 16.43570868404373 >100 2.7171395577960467 34.01531318985525 >500 0.15654449396890782 7.369949409041697 >1k 0.0877652197308522 11.83958017748516 >5k 0.008239183893100412 3.8694545818168655 >10k+ 0.0060898315731611734 8.707698081905486 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 63688 1.3699699775923608 No Hit CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 33298 0.716261467056124 No Hit CCTAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC 33032 0.7105396354074687 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 32956 0.7089048263649956 No Hit CCTAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG 26672 0.5737319313268346 No Hit CCTAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA 17582 0.3782001655889475 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 17354 0.3732957384615285 No Hit CCTAGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG 15930 0.34266457956045576 No Hit CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 14350 0.30867775999325425 No Hit CCTAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 13911 0.2992345867084432 No Hit CCTAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC 13349 0.2871456040522614 No Hit CCTAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA 13060 0.28092902756180493 No Hit CCTAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG 11874 0.2554174022411081 No Hit CCTAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG 11582 0.24913629381476451 No Hit CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 11093 0.2386175882651686 No Hit CCTAGAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC 10799 0.23229345854823363 No Hit CCTAGAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 10766 0.23158360725347563 No Hit CCTAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC 9608 0.20667428000105834 No Hit CCTAGAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC 8676 0.18662635858546855 No Hit CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT 8267 0.17782850465952843 No Hit CCTAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG 8130 0.17488154625401792 No Hit CCTAGAGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 7788 0.1675249055628895 No Hit CCTAGAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCAC 7707 0.16578254329393802 No Hit CCTAGAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA 7663 0.16483607490092733 No Hit CCTAGAGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT 7426 0.15973805196584712 No Hit CCTAGAGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCATAC 7399 0.1591572645428633 No Hit CCTAGAGGGGCTCCGCAGCCTGCTGTCCGCCTGCCTTTTCGTCTCTAGCCGC 7051 0.15167155997996068 No Hit CCTAGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA 6897 0.14835892060442332 No Hit CCTAGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA 6688 0.14386319573762263 No Hit CCTAGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG 6569 0.14130342894743464 No Hit CCTAGAGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCAGT 6244 0.13431246922633305 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT 5968 0.12837553112472067 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT 5919 0.1273215095052315 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC 5760 0.12390131690321565 No Hit CCTAGAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCGG 5665 0.12185780560012441 No Hit CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 5479 0.11785682557512475 No Hit CCTAGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT 5448 0.11718999557095812 No Hit CCTAGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT 5347 0.11501742039609272 No Hit CCTAGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG 5319 0.1144151223278132 No Hit CCTAGAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG 5088 0.10944616326450717 No Hit CCTAGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG 4915 0.10572482162835153 No Hit CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC 4832 0.10393943806880869 No Hit CCTAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 4748 0.10213254386397012 No Hit CCTAGAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG 4707 0.10125060740684654 No Hit CCTAGAGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTT 4704 0.10118607547095944 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.006001470037499508 0.0 0.0 0.0 0.0 5 0.006001470037499508 0.0 0.0 0.0 0.0 6 0.007550236498789703 0.0 0.0 0.0 0.0 7 0.007550236498789703 0.0 0.0 0.0 0.0 8 0.007679300370563887 0.0 0.0 0.0 0.0 9 0.007872896178225161 0.0 0.0 0.0 0.0 10 0.009980939417203483 0.0 0.0 0.0 1.2906387177418296E-4 11 0.014455153638708492 0.0 0.0 0.0 1.2906387177418296E-4 12 0.017057941719487848 0.0 0.0 0.0 1.2906387177418296E-4 13 0.020693240774460667 0.0 0.0 0.0 1.2906387177418296E-4 14 0.025812774354836594 0.0 0.0 0.0 1.2906387177418296E-4 15 0.02934052018333093 0.0 0.0 0.0 1.2906387177418296E-4 16 0.036804714100937844 0.0 0.0 0.0 1.2906387177418296E-4 17 0.04203180090779225 0.0 0.0 0.0 1.2906387177418296E-4 18 0.04601127028749623 0.0 0.0 0.0 1.2906387177418296E-4 19 0.05280863420093653 0.0 0.0 0.0 1.2906387177418296E-4 20 0.057863635845425365 0.0 0.0 0.0 1.2906387177418296E-4 21 0.06154195619098958 0.0 0.0 0.0 1.2906387177418296E-4 22 0.06517725524596239 0.0 0.0 0.0 1.2906387177418296E-4 23 0.06818874558736 0.0 0.0 0.0 1.2906387177418296E-4 24 0.07210368303117688 0.0 0.0 0.0 1.2906387177418296E-4 25 0.0757820033767411 0.0 0.0 0.0 1.2906387177418296E-4 26 0.07926672791464404 0.0 0.0 0.0 1.2906387177418296E-4 27 0.08298806955079965 0.0 0.0 0.0 1.2906387177418296E-4 28 0.08750530506289605 0.0 0.0 0.0 1.2906387177418296E-4 29 0.09118362540846027 0.0 0.0 0.0 1.2906387177418296E-4 30 0.09552877575819109 0.0 0.0 0.0 1.2906387177418296E-4 31 0.09948673449259937 0.0 0.0 0.0 1.2906387177418296E-4 32 0.10523007678655051 0.0 0.0 0.0 1.2906387177418296E-4 33 0.10897292906800182 0.0 0.0 0.0 1.2906387177418296E-4 34 0.11456569684488307 0.0 0.0 0.0 1.2906387177418296E-4 35 0.1227182314119523 0.0 0.0 0.0 1.2906387177418296E-4 36 0.13300031986329555 0.0 0.0 0.0 1.2906387177418296E-4 37 0.14001279022969282 0.0 0.0 0.0 1.2906387177418296E-4 38 0.14582066445953107 0.0 0.0 0.0 1.2906387177418296E-4 39 0.1515640067534822 0.0 0.0 0.0 1.2906387177418296E-4 40 0.1581892855045569 0.0 0.0 0.0 1.2906387177418296E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACAC 165 0.0 46.000004 11 AACGTAT 20 6.313448E-4 46.0 42 CTAGAGG 472145 0.0 45.061768 2 CCTAGAG 473860 0.0 45.00304 1 TAGAGGG 471075 0.0 44.980057 3 AGAGGGG 361540 0.0 44.881615 4 AGGGATC 7310 0.0 44.678524 6 GAGGGGG 167840 0.0 44.535095 5 AGGGGGG 74015 0.0 44.49909 6 GAGGGGA 104025 0.0 44.48767 5 AGAGGGA 80580 0.0 44.42157 4 GAGGGAT 23545 0.0 44.378426 5 AGGGGAG 53255 0.0 44.28542 6 GAGGGGT 33905 0.0 44.100574 5 AGGGGGC 45275 0.0 43.97813 6 AGGGGGT 28680 0.0 43.914925 6 GAGGGGC 60670 0.0 43.327347 5 AGGGGAC 24350 0.0 43.12854 6 GAGGGAC 15815 0.0 43.033195 5 AGGGGAT 15460 0.0 42.831177 6 >>END_MODULE