FastQCFastQC Report
Fri 17 Jun 2016
SRR1527310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506367
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC603291.3387502615743458No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA327630.7270379886946624No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT322600.7158760038851696No Hit
CCTAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC319730.7095072372046041No Hit
CCTAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG265910.5900762188254973No Hit
CCTAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA171630.3808611238276865No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC162130.3597798403902745No Hit
CCTAGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG156460.3471976428018401No Hit
CCTAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT143760.31901529546972096No Hit
CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG137950.3061224263358932No Hit
CCTAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC118110.2620958301887086No Hit
CCTAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG117350.2604093275137156No Hit
CCTAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA117020.2596770302995739No Hit
CCTAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG112020.24858161796409395No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC109700.24343334664043118No Hit
CCTAGAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG103460.22958627204575213No Hit
CCTAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC97830.21709283775600166No Hit
CCTAGAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC95590.21212209302970664No Hit
CCTAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT86790.19259416731926185No Hit
CCTAGAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC80560.17876928354925375No Hit
CCTAGAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCAC77730.17248928016737206No Hit
CCTAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG77020.17091373161573392No Hit
CCTAGAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA72910.16179330267596936No Hit
CCTAGAGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT70520.15648969557960993No Hit
CCTAGAGGGGCTCCGCAGCCTGCTGTCCGCCTGCCTTTTCGTCTCTAGCCGC69970.15526920022270713No Hit
CCTAGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA66980.14863414364609007No Hit
CCTAGAGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCATAC66290.14710297674379383No Hit
CCTAGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA64430.14297548335499527No Hit
CCTAGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG61590.13667328914844262No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT59480.1319910251428701No Hit
CCTAGAGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCAGT59450.1319244526688572No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT57680.12799667670209727No Hit
CCTAGAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCGG55450.1230481228004732No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC54670.12131723847613832No Hit
CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT53720.1192091101323971No Hit
CCTAGAGGGGGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG52720.11699002766530113No Hit
CCTAGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT51380.11401645715939247No Hit
CCTAGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT51230.11368359478932807No Hit
CCTAGAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG48980.10869065923836208No Hit
CCTAGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG48580.10780302625152367No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC48390.10738140058277543No Hit
CCTAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC47510.10542860801173094No Hit
CCTAGAGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTT46410.10298761729792537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAC950.045.99999618
CTAGAGG4575300.045.090622
TAGAGGG4560650.045.0372623
CCTAGAG4590200.045.028431
AGAGGGG3495100.044.968814
GAGGGGG1619000.044.6575055
GAGGGGA999800.044.608225
AGGGATC69100.044.5687376
AGGGGAG510800.044.4150356
AGGGGGG707200.044.403146
AGAGGGA796300.044.2756464
GAGGGAT229900.044.259245
AGGGGGC447250.044.215546
GAGGGGT334150.044.0107735
AGGGGGT280450.043.9579246
GAGGGGC589350.043.377455
AGGGGAC233900.043.3351866
AGGGGAT149100.043.285046
AGGGATT72700.043.0577746
AGGGGGA205000.042.903416
Produced by FastQC (version 0.11.5)