FastQCFastQC Report
Fri 17 Jun 2016
SRR1527306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614194
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC235360.9003157378526613No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT122830.46985801359807267No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC98140.3754120772980123No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC92310.35311074847543833No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG88470.33842170856485787No Hit
GTTGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA85280.32621909468080795No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC81090.310191209986711No Hit
GTTGCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT77120.29500488487082444No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT73440.2809278882898515No Hit
GTTGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG72360.2767965958150007No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC70640.2702171300217199No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGA70350.26910780148680624No Hit
GTTGCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC67630.25870306488347844No Hit
GTTGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG58520.2238548478039503No Hit
GTTGCCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT52820.20205080418668242No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT51720.1978430062956307No Hit
GTTGCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA51530.19711620484172176No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA45210.17294049332222475No Hit
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT40120.15346986489908554No Hit
GTTGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA38040.14551330161418777No Hit
GTTGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA37190.14226182142564783No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG36820.14084647122593044No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC36660.14023442789632293No Hit
GTTGCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC35510.13583536646476888No Hit
GTTGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC35080.13419050001644867No Hit
GTTGCCGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCT34350.13139805232511437No Hit
GTTGCCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT33550.12833783567707677No Hit
GTTGCCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA33430.1278788031798711No Hit
GTTGCCGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT30610.11709153949553859No Hit
GTTGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA30550.11686202324693577No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT29550.11303675243688877No Hit
GTTGCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC28520.10909672350254036No Hit
GTTGCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC28030.10722234080561732No Hit
GTTGCCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG27160.10389435520087645No Hit
GTTGCCGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCA26870.10278502666596283No Hit
GTTGCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT26790.10247900500115906No Hit
GTTGCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG26700.10213473062825483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC207.858324E-444.00000442
TATTACG207.858324E-444.00000435
CGAATAG207.858324E-444.00000432
CGTAATA207.858324E-444.00000420
GTACGTA207.858324E-444.00000410
TATCACG254.4444023E-544.025
AATTTCG302.528961E-644.034
TTATCGC302.528961E-644.029
CGTTATG254.4444023E-544.039
ATATCGT254.4444023E-544.017
TAAGGTA302.528961E-644.033
ACCGTTA600.044.032
ATAACGA351.4470243E-744.030
ACGTAAC502.7284841E-1144.011
CGCAATA502.7284841E-1144.011
GACGAAT254.4444023E-544.044
TATCGCG254.4444023E-544.029
GCCGGAT21250.042.2399984
CGGGATA24300.041.8271646
TATCGGG850.041.41176211
Produced by FastQC (version 0.11.5)