##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2614194
Sequences flagged as poor quality	0
Sequence length	50
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.471382384015875	33.0	31.0	34.0	31.0	34.0
2	32.249867071839354	33.0	31.0	34.0	31.0	34.0
3	32.85415542993366	34.0	31.0	34.0	31.0	34.0
4	36.27456837556815	37.0	37.0	37.0	35.0	37.0
5	36.26708193806581	37.0	37.0	37.0	35.0	37.0
6	36.4191984221523	37.0	37.0	37.0	35.0	37.0
7	36.47240449637632	37.0	37.0	37.0	35.0	37.0
8	36.61098372959314	37.0	37.0	37.0	35.0	37.0
9	38.65118273548176	39.0	39.0	39.0	38.0	39.0
10	38.13795609660186	39.0	39.0	39.0	37.0	39.0
11	37.856260476460434	39.0	38.0	39.0	35.0	39.0
12	37.33973721919644	39.0	37.0	39.0	35.0	39.0
13	37.20362681576042	39.0	37.0	39.0	34.0	39.0
14	37.95942917778864	40.0	38.0	40.0	34.0	40.0
15	37.94785314326328	40.0	38.0	40.0	34.0	40.0
16	37.703590475687726	40.0	37.0	40.0	33.0	40.0
17	37.74360433846914	40.0	37.0	40.0	33.0	40.0
18	37.73261969081101	40.0	37.0	40.0	33.0	40.0
19	37.579181575659646	40.0	37.0	40.0	33.0	40.0
20	37.35266472189899	40.0	36.0	40.0	32.0	40.0
21	37.58259333469513	40.0	37.0	40.0	33.0	40.0
22	37.35961753412333	40.0	36.0	40.0	33.0	40.0
23	37.35128532924488	39.0	36.0	40.0	33.0	40.0
24	37.3485965463925	39.0	36.0	40.0	33.0	40.0
25	37.25779188537653	39.0	36.0	40.0	33.0	40.0
26	37.1063628024546	39.0	35.0	40.0	33.0	40.0
27	36.914914501372124	39.0	35.0	40.0	32.0	40.0
28	36.691128890969836	39.0	35.0	40.0	31.0	40.0
29	37.00150753922624	39.0	35.0	40.0	32.0	40.0
30	37.04664726489312	39.0	35.0	40.0	32.0	40.0
31	37.02024486323509	39.0	35.0	40.0	32.0	40.0
32	36.2292729613793	39.0	35.0	40.0	30.0	40.0
33	36.28820929127678	39.0	35.0	40.0	30.0	40.0
34	36.20668205955641	39.0	35.0	40.0	30.0	40.0
35	36.248033619540095	39.0	35.0	40.0	31.0	40.0
36	36.019557079543446	38.0	35.0	40.0	30.0	40.0
37	35.85505169088446	38.0	35.0	40.0	30.0	40.0
38	35.65843468388344	38.0	35.0	40.0	29.0	40.0
39	35.53246507336487	37.0	35.0	40.0	29.0	40.0
40	35.29276480628446	37.0	34.0	40.0	29.0	40.0
41	35.13378693394599	37.0	34.0	40.0	28.0	40.0
42	34.936787399863974	36.0	34.0	40.0	28.0	40.0
43	34.855107539838286	36.0	34.0	40.0	28.0	40.0
44	34.654098739420256	35.0	34.0	40.0	27.0	40.0
45	34.53408507555292	35.0	34.0	39.0	27.0	40.0
46	34.20860961351759	35.0	33.0	39.0	26.0	40.0
47	34.089549972190284	35.0	33.0	39.0	26.0	40.0
48	33.947959103264715	35.0	33.0	39.0	26.0	40.0
49	33.67221904724745	35.0	33.0	38.0	25.0	40.0
50	33.16079258081076	35.0	32.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	12.0
15	20.0
16	71.0
17	227.0
18	422.0
19	833.0
20	1662.0
21	2860.0
22	4515.0
23	6754.0
24	9519.0
25	13482.0
26	18285.0
27	24866.0
28	32869.0
29	41723.0
30	52629.0
31	64065.0
32	77835.0
33	106639.0
34	193782.0
35	241364.0
36	324619.0
37	499730.0
38	658450.0
39	236958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.6580567471274	0.538330361097914	0.3310771886095676	0.4725357031651055
8	98.17951536879053	1.2124195832443958	0.29554042278423104	0.3125246251808397
9	93.94700622830592	3.477668451538027	1.243442529513877	1.3318827906421635
10	54.71124178236199	29.473902854952616	6.675174068948212	9.13968129373719
11	36.90992328801917	20.40349721558538	18.97602090740014	23.71055858899531
12	29.958832435542277	16.356475456679956	27.9911131308541	25.69357897692367
13	27.968620538491024	15.35398673549094	28.300807055635502	28.376585670382536
14	25.98976969574561	16.831000300666286	32.85953529080091	24.31969471278719
15	29.341739748465496	15.385965999462933	29.178553695708885	26.09374055636269
16	34.28743237877526	16.384782460674305	24.037389727005724	25.290395433544717
17	31.27308072775012	17.48236741420109	22.368959610495626	28.87559224755317
18	32.23593964334705	18.448822084359463	21.13454472009346	28.180693552200026
19	34.741721540176435	19.17539402201979	21.078198481061467	25.004685956742307
20	33.60305317814974	21.387968911259073	21.246701660244035	23.762276250347146
21	34.14027421071275	18.08813729968013	25.004379935077502	22.767208554529617
22	33.164294616237356	16.86646056107542	24.096375402896648	25.87286941979057
23	30.952790802824886	20.34263715699753	22.302132129444104	26.402439910733484
24	31.225494358873135	19.214258773449867	21.89160406610986	27.668642801567138
25	28.260182679632806	18.03997714018164	23.93116195661072	29.76867822357484
26	27.8687044649326	17.26907031383287	24.0924353739623	30.76978984727224
27	27.938898184296956	17.8331447474824	22.026330104039715	32.20162696418093
28	29.09275287143953	22.29023553722486	20.577279268485814	28.039732322849797
29	30.856776505492707	19.367996407305654	22.238058843375814	27.53716824382582
30	30.047234443962463	19.798836658641246	22.175515665631547	27.978413231764744
31	34.26272112934235	19.414167425982924	20.554518907166035	25.768592537508695
32	35.304801403415354	18.542579471913715	22.10742584521271	24.045193279458218
33	34.202052334295004	18.44029173045306	23.257570019669544	24.100085915582394
34	28.95726177934767	21.789239819233003	26.455687680409333	22.797810721009995
35	29.046849621718966	25.46945636016302	23.547028261865798	21.936665756252214
36	31.474825510271998	24.19579419124977	19.909080963386803	24.42029933509143
37	30.519655389003265	23.773254777571978	20.718470014084648	24.988619819340112
38	32.68456740394936	22.741808756350906	19.93723495654875	24.636388883150985
39	31.85245624463984	20.53883529684484	19.531603239851364	28.077105218663956
40	29.212369089669703	21.28021103254005	21.98662379303143	27.52079608475882
41	27.592825934112003	21.551575743804783	21.657535745243084	29.19806257684013
42	26.70031374871184	18.75201304876379	24.000246347440168	30.547426855084204
43	26.33565068239006	18.37759554187639	24.888741998489785	30.398011777243774
44	28.872646789029428	18.50562735588866	23.74104599735138	28.880679857730527
45	27.542064590462683	20.649882908460505	23.062710724605747	28.74534177647107
46	26.465786395347855	21.38452616753003	23.06362878960016	29.086058647521952
47	27.127979025275096	20.339079655144186	25.30875673343294	27.224184586147775
48	29.311864383439023	20.182855595261866	24.686461678054496	25.818818343244608
49	28.652349443078823	19.365548233987226	23.28912850385243	28.692973819081523
50	25.547109357606974	20.75764078717953	24.660947121751484	29.034302733462013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	53.0
8	106.0
9	199.0
10	292.0
11	379.0
12	466.0
13	1026.0
14	1586.0
15	1994.5
16	2403.0
17	2495.0
18	2587.0
19	2366.5
20	2146.0
21	2799.5
22	3453.0
23	3836.0
24	4219.0
25	3870.0
26	3521.0
27	4484.5
28	5448.0
29	6686.5
30	7925.0
31	9402.0
32	10879.0
33	13241.0
34	15603.0
35	16879.5
36	18156.0
37	22748.0
38	27340.0
39	27205.0
40	27070.0
41	29714.0
42	32358.0
43	34315.0
44	36272.0
45	39256.5
46	42241.0
47	50551.5
48	58862.0
49	64004.5
50	69147.0
51	79057.5
52	88968.0
53	93550.5
54	98133.0
55	113719.5
56	129306.0
57	152006.5
58	174707.0
59	209164.0
60	243621.0
61	260163.0
62	276705.0
63	280260.5
64	283816.0
65	292459.0
66	301102.0
67	251973.5
68	202845.0
69	172065.0
70	141285.0
71	121382.0
72	101479.0
73	86330.0
74	71181.0
75	60721.0
76	50261.0
77	49661.5
78	49062.0
79	34242.0
80	19422.0
81	12741.0
82	6060.0
83	4310.5
84	2561.0
85	1577.5
86	594.0
87	478.5
88	363.0
89	426.0
90	489.0
91	284.0
92	79.0
93	60.5
94	42.0
95	37.5
96	33.0
97	16.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2614194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.689770190045742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.0531561120472	5.2331313120894976
2	10.697237647626705	1.6451859835710976
3	4.7203534047196	1.0889529869428112
4	2.6103878466047834	0.8029313058911663
5	1.4803324847789483	0.5691708306404749
6	0.9049199114330507	0.41751756955900227
7	0.6387771382912941	0.34384345772805835
8	0.4464786004059217	0.2746654265515841
9	0.3527756349416928	0.2441487205214179
>10	3.7847947362418033	7.542808752313608
>50	2.8938048936939245	16.478716446643936
>100	3.2106145675902336	44.665117389825085
>500	0.14414580907462612	7.439999646914765
>1k	0.05340654077225357	7.473891047051303
>5k	0.007777651568774791	4.351735522002461
>10k+	0.001037020209169972	1.428183601753735
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	23536	0.9003157378526613	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	12283	0.46985801359807267	No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	9814	0.3754120772980123	No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	9231	0.35311074847543833	No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	8847	0.33842170856485787	No Hit
GTTGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	8528	0.32621909468080795	No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	8109	0.310191209986711	No Hit
GTTGCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT	7712	0.29500488487082444	No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	7344	0.2809278882898515	No Hit
GTTGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	7236	0.2767965958150007	No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	7064	0.2702171300217199	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGA	7035	0.26910780148680624	No Hit
GTTGCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	6763	0.25870306488347844	No Hit
GTTGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	5852	0.2238548478039503	No Hit
GTTGCCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	5282	0.20205080418668242	No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	5172	0.1978430062956307	No Hit
GTTGCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	5153	0.19711620484172176	No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	4521	0.17294049332222475	No Hit
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	4012	0.15346986489908554	No Hit
GTTGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	3804	0.14551330161418777	No Hit
GTTGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3719	0.14226182142564783	No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	3682	0.14084647122593044	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	3666	0.14023442789632293	No Hit
GTTGCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	3551	0.13583536646476888	No Hit
GTTGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	3508	0.13419050001644867	No Hit
GTTGCCGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCT	3435	0.13139805232511437	No Hit
GTTGCCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	3355	0.12833783567707677	No Hit
GTTGCCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA	3343	0.1278788031798711	No Hit
GTTGCCGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	3061	0.11709153949553859	No Hit
GTTGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3055	0.11686202324693577	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	2955	0.11303675243688877	No Hit
GTTGCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	2852	0.10909672350254036	No Hit
GTTGCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2803	0.10722234080561732	No Hit
GTTGCCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2716	0.10389435520087645	No Hit
GTTGCCGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCA	2687	0.10278502666596283	No Hit
GTTGCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	2679	0.10247900500115906	No Hit
GTTGCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2670	0.10213473062825483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0013005920754159792	0.0	0.0	0.0	0.0
8	0.0021038989455258485	0.0	0.0	0.0	0.0
9	0.002486426026530548	0.0	0.0	0.0	0.0
10	0.006273444128477076	0.0	0.0	0.0	0.0
11	0.011016779932935351	0.0	0.0	0.0	0.0
12	0.01545409407258987	0.0	0.0	0.0	0.0
13	0.022989877568382455	0.0	0.0	0.0	0.0
14	0.038367466224771385	0.0	0.0	0.0	0.0
15	0.0462475240934682	0.0	0.0	0.0	0.0
16	0.05512215237277723	0.0	0.0	0.0	0.0
17	0.06250492503616793	0.0	0.0	0.0	0.0
18	0.06782205146213326	0.0	0.0	0.0	0.0
19	0.07776775556825545	0.0	0.0	0.0	0.0
20	0.0844619794858377	0.0	0.0	0.0	0.0
21	0.08997036945230538	0.0	0.0	0.0	0.0
22	0.09494322150536648	0.0	0.0	0.0	0.0
23	0.10289978479026422	0.0	0.0	0.0	0.0
24	0.11074158995086057	0.0	0.0	0.0	0.0
25	0.11678551783073483	0.0	0.0	0.0	0.0
26	0.12336498362401566	0.0	0.0	0.0	0.0
27	0.1340374891840468	0.0	0.0	0.0	0.0
28	0.17087484708479936	0.0	0.0	0.0	0.0
29	0.17856364141299383	0.0	0.0	0.0	0.0
30	0.19099577154564656	0.0	0.0	0.0	0.0
31	0.1996408835763528	0.0	0.0	0.0	0.0
32	0.20893629164476699	0.0	0.0	0.0	0.0
33	0.215286241189445	0.0	0.0	0.0	0.0
34	0.22844517277600668	0.0	0.0	0.0	0.0
35	0.27102043689182975	0.0	0.0	0.0	0.0
36	0.28119565724655476	0.0	0.0	0.0	0.0
37	0.28926697865575396	0.0	0.0	0.0	0.0
38	0.29714703652445074	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGAC	20	7.858324E-4	44.000004	42
TATTACG	20	7.858324E-4	44.000004	35
CGAATAG	20	7.858324E-4	44.000004	32
CGTAATA	20	7.858324E-4	44.000004	20
GTACGTA	20	7.858324E-4	44.000004	10
TATCACG	25	4.4444023E-5	44.0	25
AATTTCG	30	2.528961E-6	44.0	34
TTATCGC	30	2.528961E-6	44.0	29
CGTTATG	25	4.4444023E-5	44.0	39
ATATCGT	25	4.4444023E-5	44.0	17
TAAGGTA	30	2.528961E-6	44.0	33
ACCGTTA	60	0.0	44.0	32
ATAACGA	35	1.4470243E-7	44.0	30
ACGTAAC	50	2.7284841E-11	44.0	11
CGCAATA	50	2.7284841E-11	44.0	11
GACGAAT	25	4.4444023E-5	44.0	44
TATCGCG	25	4.4444023E-5	44.0	29
GCCGGAT	2125	0.0	42.239998	4
CGGGATA	2430	0.0	41.827164	6
TATCGGG	85	0.0	41.411762	11
>>END_MODULE