FastQCFastQC Report
Fri 17 Jun 2016
SRR1527305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799703
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC222540.7948700272850371No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT114070.40743607446932767No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC95390.3407147115247582No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA94910.33900024395444806No Hit
GTTGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA84210.30078190436628455No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC75550.2698500519519392No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT74330.2654924468774009No Hit
GTTGCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC73930.2640637239021425No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG69610.24863351576935125No Hit
GTTGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC69340.2476691277610518No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT68040.24302577809146184No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAG67330.24048979481037808No Hit
GTTGCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT62030.22155921538820367No Hit
GTTGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG59150.21127240996634283No Hit
GTTGCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG51420.18366233846947336No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG47900.17108957628719904No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47880.1710181401384361No Hit
GTTGCCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG41190.1471227483772386No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC36290.1296208919303226No Hit
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT35990.12854934969887877No Hit
GTTGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG35820.12794214243439392No Hit
GTTGCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA34980.1249418241863512No Hit
GTTGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG34210.12219153245897868No Hit
GTTGCCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT33090.11819110812825502No Hit
GTTGCCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG32940.11765533701253311No Hit
GTTGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC28970.10347526148309302No Hit
GTTGCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC28300.10108215049953514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA551.8189894E-1246.00000413
ACGTAAC551.8189894E-1246.00000411
AATTTCG253.418423E-546.034
CGTATAC405.6170393E-946.038
CGATTTA206.313015E-446.039
TACGGTA206.313015E-446.038
TATGCGA301.8623687E-646.037
CATTACG206.313015E-446.040
ATATCGC206.313015E-446.012
ATAACGA405.6170393E-946.030
ATACGCA750.046.014
GCCGGAT21550.043.651974
CGGGATA24300.043.4444436
GTTGCCG2949900.043.087091
TTGCCGG2907650.043.011542
TCCGATA750.042.9333331
CCGGGAC139500.042.8673865
TGCCGGG2756650.042.809463
GCCGGGA812950.042.5766644
ACGTTAT650.042.4615426
Produced by FastQC (version 0.11.5)