##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2799703
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40858798236813	33.0	31.0	34.0	31.0	34.0
2	32.081950478318596	31.0	31.0	34.0	30.0	34.0
3	32.78719314155823	34.0	31.0	34.0	31.0	34.0
4	36.20812350452887	37.0	37.0	37.0	35.0	37.0
5	36.279974697316106	37.0	37.0	37.0	35.0	37.0
6	36.45962696757478	37.0	37.0	37.0	35.0	37.0
7	36.47085565861808	37.0	37.0	37.0	35.0	37.0
8	36.573736928524205	37.0	37.0	37.0	35.0	37.0
9	38.617000088938006	39.0	39.0	39.0	38.0	39.0
10	38.053416023056734	39.0	38.0	39.0	37.0	39.0
11	37.81425851242078	39.0	38.0	39.0	35.0	39.0
12	37.3417055309081	39.0	37.0	39.0	35.0	39.0
13	37.250375486256935	39.0	37.0	39.0	35.0	39.0
14	38.03267668034788	40.0	38.0	40.0	34.0	40.0
15	37.923488670048215	40.0	38.0	40.0	34.0	40.0
16	32.77433606350388	39.0	34.0	40.0	3.0	40.0
17	34.38703498192487	39.0	34.0	40.0	17.0	40.0
18	36.41421750807139	39.0	34.0	40.0	30.0	40.0
19	37.251859215066744	39.0	36.0	40.0	32.0	40.0
20	37.56592217103029	39.0	37.0	40.0	33.0	40.0
21	37.796968106974205	40.0	37.0	40.0	33.0	40.0
22	37.782167608492756	40.0	37.0	40.0	34.0	40.0
23	37.694809056532065	40.0	37.0	40.0	33.0	40.0
24	37.62920959830382	40.0	36.0	40.0	33.0	40.0
25	37.55068019714948	40.0	36.0	40.0	33.0	40.0
26	37.446750958941	40.0	36.0	40.0	33.0	40.0
27	37.272995028401226	39.0	35.0	40.0	33.0	40.0
28	37.130518487139526	39.0	35.0	40.0	33.0	40.0
29	36.88006870728788	39.0	35.0	40.0	32.0	40.0
30	36.83331874845296	39.0	35.0	40.0	32.0	40.0
31	36.779479109034064	39.0	35.0	40.0	32.0	40.0
32	36.60585712127322	39.0	35.0	40.0	31.0	40.0
33	36.125481881470996	38.0	35.0	40.0	30.0	40.0
34	35.94588747449283	38.0	35.0	40.0	30.0	40.0
35	35.93301003713608	38.0	34.0	40.0	30.0	40.0
36	35.85665836697678	38.0	34.0	40.0	30.0	40.0
37	35.45212224296649	37.0	34.0	40.0	29.0	40.0
38	35.45029133447369	37.0	34.0	40.0	29.0	40.0
39	34.99693681794105	37.0	34.0	40.0	28.0	40.0
40	34.80436639172083	36.0	34.0	40.0	27.0	40.0
41	34.71865765761583	36.0	33.0	39.0	27.0	40.0
42	34.581229866167945	36.0	33.0	39.0	27.0	40.0
43	34.51095419764168	35.0	33.0	39.0	27.0	40.0
44	34.7900966638247	35.0	34.0	39.0	28.0	40.0
45	34.870280883365126	35.0	34.0	39.0	29.0	40.0
46	34.79200579490039	35.0	34.0	39.0	29.0	40.0
47	34.49339769254096	35.0	34.0	39.0	28.0	40.0
48	34.41027994755158	35.0	34.0	39.0	28.0	40.0
49	34.33442047245726	35.0	34.0	38.0	28.0	40.0
50	33.81163251959226	35.0	33.0	38.0	27.0	40.0
51	33.900543736246306	35.0	33.0	37.0	27.0	40.0
52	33.52459028689829	35.0	33.0	37.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	2.0
14	14.0
15	29.0
16	66.0
17	132.0
18	392.0
19	804.0
20	1535.0
21	2879.0
22	4633.0
23	7058.0
24	10274.0
25	14373.0
26	20283.0
27	26827.0
28	34329.0
29	43310.0
30	55354.0
31	71402.0
32	97453.0
33	145118.0
34	230568.0
35	305126.0
36	421008.0
37	569646.0
38	560713.0
39	176374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.67807406714212	0.5243413319198501	0.3310351133673822	0.4665494875706459
8	98.174306346066	1.2093068443331312	0.3013891116307694	0.3149976979701061
9	93.90163885240685	3.4729040901838517	1.232380720383555	1.3930763370257488
10	54.88182139319778	29.38704569734718	6.611451286082845	9.119681623372193
11	37.06521727483237	20.325691689439914	18.803994566566526	23.805096469161192
12	30.194809949483925	16.2381152572255	27.96714508646096	25.599929706829617
13	28.170809546584046	15.181503180873113	28.252246756173783	28.395440516369057
14	26.257392301969173	16.804961097659287	32.71704177193081	24.22060482844073
15	29.54931290926216	15.482570829834449	29.041401891557783	25.926714369345603
16	32.14215936476119	24.65247206578698	21.374160044833328	21.831208524618503
17	31.40665277709814	17.383486748415812	22.240644811253194	28.969215663232852
18	32.35814656054588	18.372948844931052	21.101452546930872	28.16745204759219
19	34.827265606387535	19.149924116950974	21.06201979281374	24.96079048384775
20	33.638960989790704	21.38223232964354	21.262612498540022	23.716194182025735
21	34.3329631750225	18.024911928158094	24.911320950829428	22.730803945989987
22	33.02557449843787	16.81717667909775	24.147597084405025	26.009651738059357
23	31.12083674589769	20.32708469434079	22.11756032693468	26.434518232826836
24	31.276103215233903	19.267508017814748	21.840459505883302	27.61592926106805
25	28.32336144226727	17.97083476354456	23.85660193241926	29.849201861768908
26	27.91906855834351	17.17478603980494	24.02186946258228	30.884275939269273
27	27.974181547114103	17.847035917738417	21.885642869975854	32.29313966517163
28	29.220206571911376	22.262182810105216	20.44141825043585	28.07619236754756
29	31.20870320887608	19.234290208639987	22.03569450045237	27.521312082031557
30	30.259316791816847	19.822888356372086	21.974973774003885	27.942821077807185
31	34.51866144373171	19.317656194246318	20.535106759538422	25.62857560248355
32	35.53598363826449	18.05077181401027	22.170744539688673	24.242500008036565
33	34.435402612348526	18.36037608274878	23.318366269565022	23.885855035337677
34	29.019542430036328	21.71519621902752	26.39472829796589	22.870533052970263
35	29.069261989575324	25.530529488306435	23.487491351761243	21.912717170357
36	31.43694170417362	24.161562851488174	19.965403473154115	24.436091971184087
37	30.86020195713617	23.784308549871184	20.529749048381206	24.825740444611448
38	32.67743042744177	22.754306438932986	19.847533827695298	24.72072930592995
39	32.131515378595516	20.48349414205721	19.48631694147558	27.898673537871694
40	29.253888716053094	21.29600889808669	21.871998565562134	27.578103820298082
41	27.549315052346625	21.5558936072862	21.628365580206186	29.266425760160992
42	26.64871952489246	18.712734886521893	24.037263952640693	30.601281635944954
43	26.360010329667112	18.265008824150275	24.942252803243772	30.43272804293884
44	28.90574464505699	18.3628406298811	23.789094771838297	28.94231995322361
45	27.381190076233086	20.640725105484403	22.976937196552637	29.001147621729878
46	26.162560814486397	21.348550185501818	23.16949333554309	29.319395664468693
47	27.43812468679713	20.285866036504586	25.19006480330235	27.08594447339593
48	29.511987521533534	20.16006697853308	24.63372007673671	25.694225423196677
49	28.822950148640768	19.209573301168017	23.32247384811889	28.645002702072325
50	25.536565842876907	20.92275502080042	24.464023505350387	29.076655630972287
51	25.74473078037206	21.584110886047554	24.84588543856259	27.825272895017793
52	27.083087027445412	20.036482441173227	24.894676328167666	27.9857542032137
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	65.0
8	130.0
9	205.5
10	281.0
11	360.5
12	440.0
13	1023.5
14	2044.5
15	2482.0
16	2567.5
17	2653.0
18	2489.0
19	2325.0
20	2957.0
21	3589.0
22	3778.5
23	3968.0
24	3949.0
25	3930.0
26	4135.0
27	4340.0
28	5867.5
29	7395.0
30	7849.5
31	8304.0
32	9921.0
33	11538.0
34	15233.0
35	18928.0
36	20919.0
37	22910.0
38	23841.5
39	26493.5
40	28214.0
41	29356.0
42	30498.0
43	35521.0
44	40544.0
45	43855.0
46	47166.0
47	53715.5
48	60265.0
49	68633.5
50	77002.0
51	80019.5
52	83037.0
53	95906.5
54	108776.0
55	122651.5
56	136527.0
57	160534.5
58	184542.0
59	218257.5
60	251973.0
61	269653.0
62	287333.0
63	293195.5
64	297753.5
65	296449.0
66	264479.5
67	232510.0
68	197844.0
69	163178.0
70	137494.5
71	111811.0
72	98778.5
73	85746.0
74	73575.0
75	61404.0
76	57531.5
77	53659.0
78	40597.0
79	27535.0
80	17811.0
81	8087.0
82	5578.0
83	3069.0
84	2049.0
85	1029.0
86	679.0
87	329.0
88	257.5
89	128.5
90	71.0
91	59.0
92	47.0
93	39.0
94	31.0
95	32.5
96	34.0
97	17.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2799703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.336671981052898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.97713423891508	5.833764143239407
2	10.303561550126599	1.7179482575998872
3	4.408303608808508	1.1025174353858478
4	2.34475973523746	0.7818997114822057
5	1.3373780583524306	0.5574641093570818
6	0.8255275462546199	0.41292914186689444
7	0.5366899352520319	0.31319455620100956
8	0.39783976301708884	0.26533276842346315
9	0.29706300677051223	0.2228865160635951
>10	3.6571784673346937	8.035961939151097
>50	2.808054568774441	17.263426921229946
>100	2.9205948913394972	43.41593490606454
>500	0.12592617592942323	7.026710066585881
>1k	0.05255186869495616	7.752026281175029
>5k	0.006445040500324811	3.9067633408071463
>10k+	9.915446923576632E-4	1.39123990536699
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	22254	0.7948700272850371	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	11407	0.40743607446932767	No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	9539	0.3407147115247582	No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9491	0.33900024395444806	No Hit
GTTGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	8421	0.30078190436628455	No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	7555	0.2698500519519392	No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7433	0.2654924468774009	No Hit
GTTGCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	7393	0.2640637239021425	No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6961	0.24863351576935125	No Hit
GTTGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6934	0.2476691277610518	No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6804	0.24302577809146184	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAG	6733	0.24048979481037808	No Hit
GTTGCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	6203	0.22155921538820367	No Hit
GTTGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5915	0.21127240996634283	No Hit
GTTGCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	5142	0.18366233846947336	No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	4790	0.17108957628719904	No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4788	0.1710181401384361	No Hit
GTTGCCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	4119	0.1471227483772386	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3629	0.1296208919303226	No Hit
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3599	0.12854934969887877	No Hit
GTTGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	3582	0.12794214243439392	No Hit
GTTGCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	3498	0.1249418241863512	No Hit
GTTGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	3421	0.12219153245897868	No Hit
GTTGCCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	3309	0.11819110812825502	No Hit
GTTGCCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG	3294	0.11765533701253311	No Hit
GTTGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2897	0.10347526148309302	No Hit
GTTGCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2830	0.10108215049953514	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.929518595365294E-4	0.0	0.0	0.0	0.0
8	0.0019287760165989036	0.0	0.0	0.0	0.0
9	0.0023573929091764375	0.0	0.0	0.0	0.0
10	0.0052148388596933316	0.0	0.0	0.0	0.0
11	0.009179545116035522	0.0	0.0	0.0	0.0
12	0.013179969446759175	0.0	0.0	0.0	0.0
13	0.02035930239743287	0.0	0.0	0.0	0.0
14	0.0353251755632651	0.0	0.0	0.0	0.0
15	0.04261166273708318	0.0	0.0	0.0	0.0
16	0.053005622382088384	0.0	0.0	0.0	0.0
17	0.06018495533276208	0.0	0.0	0.0	0.0
18	0.06693567139085825	0.0	0.0	0.0	0.0
19	0.07707960451519322	0.0	0.0	0.0	0.0
20	0.08440180976339276	0.0	0.0	0.0	0.0
21	0.0893309040280344	0.0	0.0	0.0	0.0
22	0.09433143444143896	0.0	0.0	0.0	0.0
23	0.10218941080536043	0.0	0.0	0.0	0.0
24	0.10951161605355997	0.0	0.0	0.0	0.0
25	0.1150836356570679	0.0	0.0	0.0	0.0
26	0.12308448431851521	0.0	0.0	0.0	0.0
27	0.13401421507924233	0.0	0.0	0.0	0.0
28	0.1712324485847249	0.0	0.0	0.0	0.0
29	0.17834034538663565	0.0	0.0	0.0	0.0
30	0.18937723037050716	0.0	0.0	0.0	0.0
31	0.19852105741216122	0.0	0.0	0.0	0.0
32	0.20684336874304168	0.0	0.0	0.0	0.0
33	0.21341549442923052	0.0	0.0	0.0	0.0
34	0.2283456495206813	0.0	0.0	0.0	0.0
35	0.2704929772908055	0.0	0.0	0.0	0.0
36	0.27970824048122245	0.0	0.0	0.0	0.0
37	0.28756621684514394	0.0	0.0	0.0	0.0
38	0.2950670124652508	0.0	0.0	0.0	0.0
39	0.304067967209379	0.0	0.0	0.0	0.0
40	0.31039006637489763	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATA	55	1.8189894E-12	46.000004	13
ACGTAAC	55	1.8189894E-12	46.000004	11
AATTTCG	25	3.418423E-5	46.0	34
CGTATAC	40	5.6170393E-9	46.0	38
CGATTTA	20	6.313015E-4	46.0	39
TACGGTA	20	6.313015E-4	46.0	38
TATGCGA	30	1.8623687E-6	46.0	37
CATTACG	20	6.313015E-4	46.0	40
ATATCGC	20	6.313015E-4	46.0	12
ATAACGA	40	5.6170393E-9	46.0	30
ATACGCA	75	0.0	46.0	14
GCCGGAT	2155	0.0	43.65197	4
CGGGATA	2430	0.0	43.444443	6
GTTGCCG	294990	0.0	43.08709	1
TTGCCGG	290765	0.0	43.01154	2
TCCGATA	75	0.0	42.93333	31
CCGGGAC	13950	0.0	42.867386	5
TGCCGGG	275665	0.0	42.80946	3
GCCGGGA	81295	0.0	42.576664	4
ACGTTAT	65	0.0	42.46154	26
>>END_MODULE