FastQCFastQC Report
Fri 17 Jun 2016
SRR1527304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714855
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC206310.7599300883472598No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT110760.4079775899633682No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC93700.3451381381326074No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA92120.33931830613421343No Hit
GTTGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA82440.30366262654911585No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC74860.27574216670871926No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT72840.2683016220019117No Hit
GTTGCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC72040.26535487162297805No Hit
GTTGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC69790.25706713618222704No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT69710.25677246114433366No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG67530.2487425663617394No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAG67520.24870573198200271No Hit
GTTGCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT59410.21883305001556252No Hit
GTTGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG57500.21179768348585834No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC48180.17746804157128096No Hit
GTTGCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG47280.17415294739498058No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG45490.16755959342211646No Hit
GTTGCCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG42720.15735647023505858No Hit
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT37050.13647137692436612No Hit
GTTGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG35310.1300621948501854No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC34020.1253105598641548No Hit
GTTGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG32560.11993274042260084No Hit
GTTGCCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT31670.11665448062603712No Hit
GTTGCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA31420.11573362113262034No Hit
GTTGCCGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG30850.1136340614876301No Hit
GTTGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC28340.10438863217372568No Hit
GTTGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT27390.10088936609874191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA206.312981E-446.013
ACGTTAC253.418395E-546.040
TTATACG206.312981E-446.044
AACGATA253.418395E-546.021
TTACGGT206.312981E-446.037
CGATATA253.418395E-546.023
TATCGAA206.312981E-446.035
CGGGATA25000.043.6086
GCCGGAT23450.043.0575684
GTTGCCG2881300.042.7567061
TTGCCGG2840450.042.7108732
TGCCGGG2687350.042.597093
GCCGGGA785550.042.4045564
CCGGGGG575300.042.2659455
CCGGGAT166150.042.2208865
CCGGGAC135900.042.1920555
ACGTAAC601.8189894E-1242.16666811
GCCGGGG1535200.042.130214
CGGGGGG255450.042.0653766
CGGGAGT91850.041.7680976
Produced by FastQC (version 0.11.5)