FastQCFastQC Report
Fri 17 Jun 2016
SRR1527300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2706137
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC342361.2651244190519548No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG197830.7310420721493406No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC143050.5286132963704351No Hit
AGGACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT138880.5132038769655787No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGA133070.4917341583223614No Hit
AGGACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT100540.3715259057468266No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC92220.3407809730253864No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT91020.3363466077290248No Hit
AGGACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC78150.288788039925547No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG74020.273526432697236No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT64060.23672120073743494No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT63520.23472573635407223No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA58150.21488195165285423No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC58120.21477109252044518No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG55890.20653056367803996No Hit
AGGACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG50110.18517170416723175No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT46270.17098173521887472No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA45990.16994704998305704No Hit
AGGACTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC45740.16902322387964838No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT43260.15985886893383447No Hit
AGGACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA39550.14614928955924994No Hit
AGGACTGGGCATATATCTCCTAACCTTTGACGACTCTGTATTTCATTTTT37490.1385369624671626No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA36360.13436126847975544No Hit
AGGACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA35040.12948346665375773No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC34570.12774667357934946No Hit
AGGACTGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGC33010.12198199869407943No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT32660.1206886421493073No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC32560.12031911170794383No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC31650.11695638469153631No Hit
AGGACTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC30750.11363061071926514No Hit
AGGACTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC30390.11230030113035666No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC29720.10982444717322147No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC27430.10136220006599815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC408.3200575E-944.00000414
CGTCTAG207.858374E-444.00000441
CCGTCTA207.858374E-444.00000429
CCGTAAG207.858374E-444.00000444
CGTACGT207.858374E-444.00000432
TCGTACG207.858374E-444.00000415
TTACGTC207.858374E-444.00000439
ACCGTAA207.858374E-444.00000443
CTCGTTA254.444445E-544.030
TCGTATT254.444445E-544.031
CGTACAC454.802132E-1044.021
GGATACG850.043.9999968
ACTGGGC335900.041.3801734
TGGGATA24700.041.3279346
CGTATAG800.041.25000425
GGTATAC750.041.06666610
TATGTCG750.041.06666640
TTATCGA700.040.85714331
AGGACTG2937150.040.3522451
CTGGGAT226700.040.283195
Produced by FastQC (version 0.11.5)