##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527300_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2706137
Sequences flagged as poor quality	0
Sequence length	50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.485145800083295	34.0	31.0	34.0	31.0	34.0
2	32.80753893834643	34.0	31.0	34.0	31.0	34.0
3	33.11461577887594	34.0	34.0	34.0	31.0	34.0
4	36.02410631834235	37.0	35.0	37.0	35.0	37.0
5	36.38078042612033	37.0	37.0	37.0	35.0	37.0
6	36.34550726737042	37.0	37.0	37.0	35.0	37.0
7	36.54270755693448	37.0	37.0	37.0	35.0	37.0
8	36.6626039258175	37.0	37.0	37.0	35.0	37.0
9	38.7388631839408	39.0	39.0	39.0	39.0	39.0
10	38.243382356473454	39.0	39.0	39.0	37.0	39.0
11	38.01716542806221	39.0	38.0	39.0	35.0	39.0
12	37.418048310192724	39.0	37.0	39.0	35.0	39.0
13	37.24077421061831	39.0	37.0	39.0	35.0	39.0
14	37.980277051753106	40.0	38.0	40.0	34.0	40.0
15	37.95753947416557	40.0	37.0	40.0	34.0	40.0
16	37.69012729215114	40.0	37.0	40.0	33.0	40.0
17	37.733301381267836	40.0	37.0	40.0	33.0	40.0
18	37.683535977668534	40.0	37.0	40.0	33.0	40.0
19	37.5331951043129	40.0	37.0	40.0	33.0	40.0
20	37.362912151158646	40.0	36.0	40.0	32.0	40.0
21	37.632472413628726	40.0	37.0	40.0	33.0	40.0
22	37.458382557867544	40.0	36.0	40.0	33.0	40.0
23	37.526247562484826	40.0	36.0	40.0	33.0	40.0
24	37.599474823336735	40.0	36.0	40.0	34.0	40.0
25	37.53533320744663	40.0	36.0	40.0	34.0	40.0
26	37.39843067812162	40.0	36.0	40.0	33.0	40.0
27	37.289980514659824	39.0	35.0	40.0	33.0	40.0
28	37.08253462407853	39.0	35.0	40.0	33.0	40.0
29	37.37866855964794	40.0	35.0	40.0	33.0	40.0
30	37.419689025352376	40.0	36.0	40.0	33.0	40.0
31	37.38975151664531	40.0	35.0	40.0	33.0	40.0
32	36.62741502000823	40.0	35.0	40.0	31.0	40.0
33	36.81304198567922	40.0	35.0	40.0	31.0	40.0
34	36.71274033797993	40.0	35.0	40.0	31.0	40.0
35	36.75818371353704	40.0	35.0	40.0	31.0	40.0
36	36.63957774495526	39.0	35.0	40.0	31.0	40.0
37	36.58219890567255	39.0	35.0	40.0	31.0	40.0
38	36.43064929824322	39.0	35.0	40.0	31.0	40.0
39	36.38623986886104	39.0	35.0	40.0	31.0	40.0
40	36.23193393386957	39.0	35.0	40.0	30.0	40.0
41	36.1046247843328	39.0	35.0	40.0	30.0	40.0
42	36.06558574085496	39.0	35.0	40.0	30.0	40.0
43	35.96252554841089	38.0	35.0	40.0	30.0	40.0
44	35.81214883060244	38.0	35.0	40.0	30.0	40.0
45	35.73579201644262	38.0	35.0	40.0	30.0	40.0
46	35.44094995929622	38.0	35.0	40.0	29.0	40.0
47	35.33064586161011	37.0	35.0	40.0	28.0	40.0
48	35.227076086687404	37.0	34.0	40.0	28.0	40.0
49	34.985551729273126	37.0	34.0	40.0	27.0	40.0
50	34.57087058046211	36.0	34.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	11.0
15	33.0
16	70.0
17	171.0
18	365.0
19	754.0
20	1348.0
21	2200.0
22	3744.0
23	5454.0
24	8139.0
25	11237.0
26	15744.0
27	21143.0
28	27832.0
29	36445.0
30	47327.0
31	59078.0
32	70544.0
33	96206.0
34	199076.0
35	220674.0
36	214648.0
37	388551.0
38	839901.0
39	435440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.52049729928677	0.355783908944743	0.08791129200036804	0.03580749976811965
8	99.27257932617603	0.5735112449960958	0.06614594900406003	0.08776347982382267
9	97.62879706385893	1.293282638683851	0.4654974969855554	0.6124228004716686
10	54.23505905281218	28.434406683770995	4.9305338199802895	12.400000443436529
11	35.51978336647406	20.99686749044856	20.021565796557972	23.46178334651941
12	31.78937356090989	14.667106654245519	28.724598939373724	24.818920845470867
13	26.48295337597468	18.359972166967157	27.051734631321327	28.105339825736834
14	28.678814117688795	16.025426650609337	31.255919415757592	24.039839815944276
15	32.94349103537626	16.823242873513056	27.853098346462136	22.380167744648553
16	34.48310266627299	19.578129266921813	24.025612893951784	21.913155172853408
17	33.53459192938125	19.29359082707195	21.566535618854477	25.60528162469232
18	33.43093864057881	19.133953676402932	25.771237745908653	21.663869937109613
19	33.341216649415756	21.24452679225036	23.29168848435981	22.122568073974083
20	33.92355967196043	23.18770261816013	21.51875533278618	21.36998237709325
21	34.44511493690083	20.003200133622208	24.54968096589345	21.002003963583515
22	31.829430660753687	18.44825298940889	25.652433709010296	24.069882640827128
23	32.17571763735539	20.77123220295203	23.53036819643647	23.522681963256108
24	32.57573434013134	20.341726970955275	22.51083370871467	24.57170498019871
25	27.989011642795614	18.814605468976627	22.031626632354534	31.16475625587323
26	27.62964328856965	19.126710879752206	25.50561926465659	27.73802656702155
27	26.129423602722255	18.069040850481702	24.15897643023986	31.642559116556185
28	25.778074059073873	24.61542043141201	21.99992092048555	27.606584589028564
29	31.946682669798314	21.19674650618206	21.508814963913505	25.34775586010612
30	29.656850336845476	20.465187091414812	24.45803741643531	25.41992515530441
31	34.263564631058955	18.901075592255676	22.417933755755897	24.417426020929465
32	35.88646842343902	18.39149311361546	23.599396482883165	22.122641980062355
33	35.853617167201804	18.108839279016546	24.00369973877893	22.033843815002715
34	29.14128146505517	20.943433388627405	27.587369006077665	22.327916140239758
35	26.772406570694685	27.29699198525426	25.969269109435334	19.96133233461573
36	29.7749153128611	27.902615425604836	21.088547992950836	21.233921268583224
37	31.17831802307126	27.115811209853753	19.876599004411087	21.8292717626639
38	31.59980444449043	26.02174243210894	19.693348858538943	22.685104264861682
39	30.576131215825363	23.209911397686074	19.56615648062164	26.647800905866927
40	28.151826755260355	23.214973964732756	20.688272618865934	27.944926661140958
41	24.50223325722238	24.341635327405818	20.875144163063435	30.280987252308368
42	24.99895607650315	21.539596849679082	22.699515952074858	30.761931121742915
43	25.91516985281972	19.2439259357527	25.135534527631087	29.705369683796494
44	26.755519029524372	20.359944821714496	23.287254119063448	29.597282029697684
45	28.015617834573785	20.335777530849324	22.1223833087534	29.52622132582349
46	25.91768265982099	22.269678142680878	24.50193763286929	27.310701564628843
47	27.452342582803457	20.213869438243517	25.520880871884906	26.812907107068117
48	27.755394497765636	20.39833903457216	26.21552419555994	25.630742272102264
49	29.411740795089088	19.63806710451097	24.609581850438467	26.340610249961475
50	25.866465740648014	20.42612772376269	25.28848317731142	28.41892335827787
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	9.0
6	18.0
7	11.5
8	5.0
9	45.0
10	85.0
11	302.5
12	520.0
13	812.0
14	1104.0
15	1793.0
16	2482.0
17	2622.5
18	2763.0
19	3143.0
20	3523.0
21	3863.0
22	4203.0
23	4445.0
24	4687.0
25	4799.0
26	4911.0
27	7381.5
28	9852.0
29	10457.0
30	11062.0
31	12645.5
32	14229.0
33	16854.5
34	19480.0
35	24146.0
36	28812.0
37	33128.0
38	37444.0
39	38066.0
40	38688.0
41	41626.0
42	44564.0
43	52742.0
44	60920.0
45	65717.5
46	70515.0
47	84491.5
48	98468.0
49	110084.5
50	121701.0
51	119232.5
52	116764.0
53	129853.0
54	142942.0
55	167815.5
56	192689.0
57	225357.0
58	258025.0
59	279015.0
60	300005.0
61	296218.0
62	292431.0
63	297689.5
64	302948.0
65	245596.5
66	188245.0
67	156294.5
68	124344.0
69	99055.0
70	73766.0
71	61868.5
72	49971.0
73	44579.0
74	39187.0
75	31800.5
76	24414.0
77	19052.5
78	13691.0
79	8774.0
80	3857.0
81	2921.5
82	1986.0
83	1222.5
84	459.0
85	379.0
86	299.0
87	175.5
88	52.0
89	35.5
90	19.0
91	12.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2706137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.263361831503742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.09445903237112	4.873313328411159
2	10.915259117193068	1.5856295290558673
3	4.845134500453916	1.0557589499729678
4	2.641817698609133	0.7675391135147454
5	1.4468817055461567	0.5254612677382495
6	0.8970989204801603	0.3909572434859285
7	0.6669653052131197	0.33910872385755525
8	0.4500774468028236	0.2615260278662628
9	0.3316820306577542	0.21682139415076673
>10	4.11487045027369	7.930146714730571
>50	3.1046636632726994	16.534290973867886
>100	3.2546755834855583	42.4292454374605
>500	0.16220370171768417	8.00595169314094
>1k	0.06572960461762366	8.381186763047747
>5k	0.005300774565937392	2.63834992822018
>10k+	0.0031804647395624348	4.064712911478695
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	34236	1.2651244190519548	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	19783	0.7310420721493406	No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	14305	0.5286132963704351	No Hit
AGGACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	13888	0.5132038769655787	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGA	13307	0.4917341583223614	No Hit
AGGACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	10054	0.3715259057468266	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	9222	0.3407809730253864	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	9102	0.3363466077290248	No Hit
AGGACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC	7815	0.288788039925547	No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	7402	0.273526432697236	No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	6406	0.23672120073743494	No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6352	0.23472573635407223	No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	5815	0.21488195165285423	No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	5812	0.21477109252044518	No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	5589	0.20653056367803996	No Hit
AGGACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG	5011	0.18517170416723175	No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	4627	0.17098173521887472	No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	4599	0.16994704998305704	No Hit
AGGACTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4574	0.16902322387964838	No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	4326	0.15985886893383447	No Hit
AGGACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA	3955	0.14614928955924994	No Hit
AGGACTGGGCATATATCTCCTAACCTTTGACGACTCTGTATTTCATTTTT	3749	0.1385369624671626	No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	3636	0.13436126847975544	No Hit
AGGACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	3504	0.12948346665375773	No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	3457	0.12774667357934946	No Hit
AGGACTGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGC	3301	0.12198199869407943	No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	3266	0.1206886421493073	No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	3256	0.12031911170794383	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC	3165	0.11695638469153631	No Hit
AGGACTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	3075	0.11363061071926514	No Hit
AGGACTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC	3039	0.11230030113035666	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	2972	0.10982444717322147	No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2743	0.10136220006599815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.760139268632742E-4	0.0	0.0	0.0	0.0
8	8.868730592723132E-4	0.0	0.0	0.0	0.0
9	0.0014781217654538555	0.0	0.0	0.0	0.0
10	0.011085913240903916	0.0	0.0	0.0	0.0
11	0.021211047334262826	0.0	0.0	0.0	0.0
12	0.02874946833807749	0.0	0.0	0.0	0.0
13	0.03669437282739196	0.0	0.0	0.0	0.0
14	0.05258418180602091	0.0	0.0	0.0	0.0
15	0.059974790633290184	0.0	0.0	0.0	0.0
16	0.06766102381365023	0.0	0.0	0.0	0.0
17	0.07279749694860238	0.0	0.0	0.0	0.0
18	0.0787838900986905	0.0	0.0	0.0	0.0
19	0.08661793545559593	0.0	0.0	0.0	0.0
20	0.09374987297391078	0.0	0.0	0.0	0.0
21	0.1001427496094987	0.0	0.0	0.0	0.0
22	0.1072377340836772	0.0	0.0	0.0	0.0
23	0.1149609203081736	0.0	0.0	0.0	0.0
24	0.12327535523885154	0.0	0.0	0.0	0.0
25	0.12966823187443946	0.0	0.0	0.0	0.0
26	0.1380565728933901	0.0	0.0	0.0	0.0
27	0.14607538347097726	0.0	0.0	0.0	0.0
28	0.18221546063632402	0.0	0.0	0.0	0.0
29	0.19211887646486486	0.0	0.0	0.0	0.0
30	0.20597626801599475	0.0	0.0	0.0	0.0
31	0.21432765599080905	0.0	0.0	0.0	0.0
32	0.22230951352425987	0.0	0.0	0.0	0.0
33	0.22733512752680296	0.0	0.0	0.0	0.0
34	0.2369059659581167	0.0	0.0	0.0	0.0
35	0.28073227630382347	0.0	0.0	0.0	0.0
36	0.2962156017969526	0.0	0.0	0.0	0.0
37	0.3054169097869029	0.0	0.0	0.0	0.0
38	0.314174781247217	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAC	40	8.3200575E-9	44.000004	14
CGTCTAG	20	7.858374E-4	44.000004	41
CCGTCTA	20	7.858374E-4	44.000004	29
CCGTAAG	20	7.858374E-4	44.000004	44
CGTACGT	20	7.858374E-4	44.000004	32
TCGTACG	20	7.858374E-4	44.000004	15
TTACGTC	20	7.858374E-4	44.000004	39
ACCGTAA	20	7.858374E-4	44.000004	43
CTCGTTA	25	4.444445E-5	44.0	30
TCGTATT	25	4.444445E-5	44.0	31
CGTACAC	45	4.802132E-10	44.0	21
GGATACG	85	0.0	43.999996	8
ACTGGGC	33590	0.0	41.380173	4
TGGGATA	2470	0.0	41.327934	6
CGTATAG	80	0.0	41.250004	25
GGTATAC	75	0.0	41.066666	10
TATGTCG	75	0.0	41.066666	40
TTATCGA	70	0.0	40.857143	31
AGGACTG	293715	0.0	40.352245	1
CTGGGAT	22670	0.0	40.28319	5
>>END_MODULE