FastQCFastQC Report
Fri 17 Jun 2016
SRR1527299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919457
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC320431.0975671160767226No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT180590.6185739334403624No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA149350.5115677333147911No Hit
AGGACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC125170.4287441123469193No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG119560.4095282102116935No Hit
AGGACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT93610.32064181798190555No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC86300.29560291519964155No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT83920.2874507142937882No Hit
AGGACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC72850.24953270419807522No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC70170.2403529149427445No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG62270.21329308840650846No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT59340.20325697552661331No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG58620.20079076348786776No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC55250.18924752102873926No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG53320.18263670264710186No Hit
AGGACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA48090.16472241242121394No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC44820.15352169941191118No Hit
AGGACTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT43150.14780145759982077No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT42990.14725341048009954No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT40750.13958075080400226No Hit
AGGACTGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC37640.12892808491442073No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG37250.12759222006010024No Hit
AGGACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA36200.1239956608369296No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC34500.11817266018989148No Hit
AGGACTGGGCATATATCTCCTAACCTTTGACGACTCTGTATTTCATTTTTGA34440.117967142519996No Hit
AGGACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA33310.11409655973696478No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG32950.11286345371759199No Hit
AGGACTGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTC31690.10854758264978728No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC29910.10245055844288853No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA29510.10108044064358543No Hit
AGGACTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC29490.10101193475362027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG405.6170393E-946.014
CGTATAG206.313061E-446.025
TAACGTT206.313061E-446.030
ATATGCG206.313061E-446.014
ACGATTT501.6370905E-1146.039
CTAACGT206.313061E-446.027
ATTTTCG301.862396E-646.010
CGTACTC253.418458E-546.013
GTATCGC206.313061E-446.018
TATACGC301.862396E-646.032
CGATCAA405.6170393E-946.010
TATAACG206.313061E-446.042
CGATAGC206.313061E-446.041
TAATGCG206.313061E-446.040
TAATCGA551.8189894E-1246.036
TGGGATA27200.043.716916
ACTGGGC369800.043.0643584
AGGACTG3164200.042.2551041
CTGGGAT240550.042.1180655
GACTGGG3084800.042.072973
Produced by FastQC (version 0.11.5)